Tomato leaf curl Mali virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6RCU1|Q6RCU1_9GEMI Transcriptional activator protein OS=Tomato leaf curl Mali virus OX=260379 GN=C2 PE=3 SV=1
MM1 pKa = 7.35PRR3 pKa = 11.84AGRR6 pKa = 11.84FSIKK10 pKa = 10.18AKK12 pKa = 10.62NYY14 pKa = 8.86FLTYY18 pKa = 8.4PQCSLTKK25 pKa = 10.31KK26 pKa = 9.61EE27 pKa = 5.12ALSQLQNLQTPTNKK41 pKa = 9.87KK42 pKa = 9.42FIKK45 pKa = 9.65ICRR48 pKa = 11.84EE49 pKa = 3.6LHH51 pKa = 6.27EE52 pKa = 5.78DD53 pKa = 4.17GEE55 pKa = 4.71HH56 pKa = 6.33HH57 pKa = 6.76LHH59 pKa = 6.72VLIQFEE65 pKa = 4.49GKK67 pKa = 10.12YY68 pKa = 10.4NCTNQRR74 pKa = 11.84FFDD77 pKa = 4.07LVSPTRR83 pKa = 11.84SAHH86 pKa = 5.07FHH88 pKa = 6.74PNVQGAKK95 pKa = 9.76SSSDD99 pKa = 3.11VKK101 pKa = 11.24SYY103 pKa = 10.79IDD105 pKa = 3.7KK106 pKa = 11.33DD107 pKa = 3.49GDD109 pKa = 3.67TLEE112 pKa = 4.14WGEE115 pKa = 3.84FQIDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGCQTANDD132 pKa = 4.06AAAEE136 pKa = 3.94ALNAGSTEE144 pKa = 3.73AALAIIKK151 pKa = 9.62EE152 pKa = 4.4KK153 pKa = 10.84LPKK156 pKa = 10.24DD157 pKa = 3.94YY158 pKa = 10.78IFQYY162 pKa = 11.19HH163 pKa = 5.53NLKK166 pKa = 10.92SNLDD170 pKa = 4.04RR171 pKa = 11.84IFQEE175 pKa = 4.04PPEE178 pKa = 4.48VYY180 pKa = 10.32VSPFSSSSFTQVPDD194 pKa = 3.73EE195 pKa = 4.28LEE197 pKa = 3.7EE198 pKa = 4.09WVADD202 pKa = 4.42NIRR205 pKa = 11.84DD206 pKa = 3.59SAARR210 pKa = 11.84PWRR213 pKa = 11.84PKK215 pKa = 10.66SIVLEE220 pKa = 4.37GDD222 pKa = 2.97SRR224 pKa = 11.84TGKK227 pKa = 7.65TVWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.18LSPKK249 pKa = 10.08VYY251 pKa = 10.98SNNAWYY257 pKa = 10.49NVIDD261 pKa = 5.17DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.01LKK269 pKa = 10.72HH270 pKa = 6.2FKK272 pKa = 10.58EE273 pKa = 4.19LMGAQRR279 pKa = 11.84DD280 pKa = 3.84WQSNTKK286 pKa = 9.6YY287 pKa = 10.57GKK289 pKa = 9.12PVQIKK294 pKa = 10.35GGIPSIFLCNPGATSSYY311 pKa = 11.19KK312 pKa = 10.63EE313 pKa = 3.93FLDD316 pKa = 3.79EE317 pKa = 4.32EE318 pKa = 4.49KK319 pKa = 11.08NRR321 pKa = 11.84ALKK324 pKa = 10.33AWAVKK329 pKa = 10.13NATFVTLTEE338 pKa = 4.29PLFSNANQGSPQTSQEE354 pKa = 4.13EE355 pKa = 4.13TDD357 pKa = 3.48QAA359 pKa = 3.99

Molecular weight: 40.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6RCU4|Q6RCU4_9GEMI Protein V2 OS=Tomato leaf curl Mali virus OX=260379 GN=V1 PE=3 SV=1
MM1 pKa = 7.83LKK3 pKa = 10.19RR4 pKa = 11.84PADD7 pKa = 3.53IFISTPASKK16 pKa = 10.33VRR18 pKa = 11.84RR19 pKa = 11.84KK20 pKa = 10.58LNFDD24 pKa = 3.32SPYY27 pKa = 9.93SSRR30 pKa = 11.84AVVPTVRR37 pKa = 11.84GTSKK41 pKa = 10.4RR42 pKa = 11.84QLWTYY47 pKa = 10.16RR48 pKa = 11.84PMYY51 pKa = 9.85RR52 pKa = 11.84KK53 pKa = 9.24PKK55 pKa = 8.36FYY57 pKa = 11.19KK58 pKa = 9.28MYY60 pKa = 10.09RR61 pKa = 11.84SPDD64 pKa = 3.34VPRR67 pKa = 11.84GCEE70 pKa = 4.4GPCKK74 pKa = 9.46IQSYY78 pKa = 7.0EE79 pKa = 3.64QRR81 pKa = 11.84DD82 pKa = 4.07DD83 pKa = 3.72VKK85 pKa = 9.5HH86 pKa = 5.48TGIVRR91 pKa = 11.84CVSDD95 pKa = 3.21ITRR98 pKa = 11.84GPGLTHH104 pKa = 5.98RR105 pKa = 11.84TGKK108 pKa = 10.08RR109 pKa = 11.84FCIKK113 pKa = 10.47SMYY116 pKa = 10.01ILGKK120 pKa = 9.5IWMDD124 pKa = 3.4EE125 pKa = 4.2NIKK128 pKa = 8.76KK129 pKa = 8.68TNHH132 pKa = 5.49TNNVMFYY139 pKa = 10.01LVRR142 pKa = 11.84DD143 pKa = 3.56RR144 pKa = 11.84RR145 pKa = 11.84PYY147 pKa = 10.66GKK149 pKa = 10.17SPMDD153 pKa = 3.86FGQVFNMFDD162 pKa = 3.99NEE164 pKa = 4.11PSTATVKK171 pKa = 10.81NDD173 pKa = 2.84LRR175 pKa = 11.84DD176 pKa = 3.47RR177 pKa = 11.84YY178 pKa = 9.28QVLRR182 pKa = 11.84KK183 pKa = 8.77FHH185 pKa = 6.05ATVTGGPSGVKK196 pKa = 9.49EE197 pKa = 3.71QALVKK202 pKa = 10.32RR203 pKa = 11.84FYY205 pKa = 10.69RR206 pKa = 11.84LNSHH210 pKa = 6.04VVYY213 pKa = 10.69NHH215 pKa = 5.41QEE217 pKa = 3.59EE218 pKa = 4.62AKK220 pKa = 10.65YY221 pKa = 10.68EE222 pKa = 4.07NHH224 pKa = 6.48TEE226 pKa = 3.99NALLLYY232 pKa = 7.29MACTHH237 pKa = 7.07ASNPVYY243 pKa = 9.86ATLKK247 pKa = 9.47IRR249 pKa = 11.84IYY251 pKa = 10.65FYY253 pKa = 11.26DD254 pKa = 3.34AVTNN258 pKa = 4.16

Molecular weight: 30.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1087

85

359

181.2

20.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.336 ± 0.989

2.116 ± 0.415

4.876 ± 0.489

5.06 ± 0.629

3.864 ± 0.562

4.508 ± 0.521

4.048 ± 0.668

6.072 ± 1.578

6.348 ± 0.776

7.636 ± 0.666

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.748 ± 0.533

6.164 ± 1.012

6.072 ± 0.748

5.244 ± 0.913

6.44 ± 0.888

7.728 ± 1.025

5.98 ± 0.604

5.244 ± 0.717

1.38 ± 0.211

4.14 ± 0.668
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski