Hubei orthoptera virus 5

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Xinmoviridae; Anphevirus; Orthopteran anphevirus

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KNE4|A0A1L3KNE4_9MONO Uncharacterized protein OS=Hubei orthoptera virus 5 OX=1923013 PE=4 SV=1
MM1 pKa = 7.92DD2 pKa = 4.8TFQIDD7 pKa = 3.76PASDD11 pKa = 3.71TPCPHH16 pKa = 6.92RR17 pKa = 11.84AIVFQPAGGRR27 pKa = 11.84SVRR30 pKa = 11.84IYY32 pKa = 10.97ARR34 pKa = 11.84PPDD37 pKa = 3.66QGAAPSYY44 pKa = 10.14PIYY47 pKa = 10.55LCPNADD53 pKa = 3.82FLTLSVDD60 pKa = 3.03ALTIGGYY67 pKa = 9.99GDD69 pKa = 3.56HH70 pKa = 6.21VQYY73 pKa = 10.81DD74 pKa = 3.51IRR76 pKa = 11.84NQIEE80 pKa = 4.0PQDD83 pKa = 3.77WVADD87 pKa = 3.75SATTFTVAPSRR98 pKa = 11.84LPVWIGIDD106 pKa = 3.29HH107 pKa = 7.17AAAKK111 pKa = 10.11YY112 pKa = 9.55VVDD115 pKa = 5.51RR116 pKa = 11.84ISPVILSAPPEE127 pKa = 4.16SDD129 pKa = 4.72PRR131 pKa = 11.84LEE133 pKa = 4.47WIASYY138 pKa = 9.73WLNLLVIYY146 pKa = 9.62SPPLARR152 pKa = 11.84SIRR155 pKa = 11.84AWALAGEE162 pKa = 4.35NPLYY166 pKa = 10.15TLQEE170 pKa = 4.22VQEE173 pKa = 4.78KK174 pKa = 10.11YY175 pKa = 10.44PPAQGTVVTPIAPLHH190 pKa = 5.3QLIHH194 pKa = 6.55TYY196 pKa = 10.54FAMTLGFKK204 pKa = 9.99NVNSDD209 pKa = 4.68YY210 pKa = 11.09ISDD213 pKa = 3.88WAQKK217 pKa = 9.85RR218 pKa = 11.84ANSMLAGLGLDD229 pKa = 3.7KK230 pKa = 11.13ASASAAIAGNFPTAAVTSTFFQSHH254 pKa = 5.61PTLLRR259 pKa = 11.84FIIDD263 pKa = 4.03FVTANLTEE271 pKa = 4.68CEE273 pKa = 4.19DD274 pKa = 3.78PGIQKK279 pKa = 10.22LCSIVDD285 pKa = 4.11MILNKK290 pKa = 10.62AGMATIEE297 pKa = 4.17SAYY300 pKa = 10.71NFAKK304 pKa = 10.49AASTAASFLPRR315 pKa = 11.84VAEE318 pKa = 3.79QSVALIEE325 pKa = 3.89IWEE328 pKa = 4.13AMMKK332 pKa = 10.72DD333 pKa = 3.55EE334 pKa = 5.81GEE336 pKa = 4.48VTQTAAGNVHH346 pKa = 6.5GLGAMFPYY354 pKa = 10.43ARR356 pKa = 11.84ALGLRR361 pKa = 11.84GVEE364 pKa = 3.9LLNVGKK370 pKa = 10.42YY371 pKa = 10.66ADD373 pKa = 4.06LAYY376 pKa = 10.46CAWFQRR382 pKa = 11.84ADD384 pKa = 3.6EE385 pKa = 4.42NPTYY389 pKa = 11.14RR390 pKa = 11.84NMAHH394 pKa = 6.38SQATDD399 pKa = 3.16QKK401 pKa = 9.72TLAAHH406 pKa = 6.08TKK408 pKa = 8.38VPLSIGYY415 pKa = 7.67TSLTEE420 pKa = 4.15TVKK423 pKa = 10.87KK424 pKa = 10.53FLDD427 pKa = 3.36EE428 pKa = 4.48RR429 pKa = 11.84GISEE433 pKa = 3.92AQYY436 pKa = 11.1TEE438 pKa = 4.07GCKK441 pKa = 9.52QAAKK445 pKa = 10.57NMEE448 pKa = 4.63LIKK451 pKa = 10.7PEE453 pKa = 3.85EE454 pKa = 3.87LRR456 pKa = 11.84SNFEE460 pKa = 3.99LLTRR464 pKa = 11.84RR465 pKa = 3.98

Molecular weight: 51.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KNA8|A0A1L3KNA8_9MONO Uncharacterized protein OS=Hubei orthoptera virus 5 OX=1923013 PE=4 SV=1
MM1 pKa = 7.5AGKK4 pKa = 9.67FVFGLTVSILVSFISGRR21 pKa = 11.84FVSKK25 pKa = 10.98HH26 pKa = 4.4EE27 pKa = 3.9VAIIADD33 pKa = 3.95EE34 pKa = 4.32FTTVTLGVKK43 pKa = 9.7ILMPSDD49 pKa = 3.63LVKK52 pKa = 10.81DD53 pKa = 3.86ILTSLPDD60 pKa = 3.55NAPPATHH67 pKa = 6.93EE68 pKa = 4.25GRR70 pKa = 11.84AKK72 pKa = 10.27LVKK75 pKa = 10.23LLSEE79 pKa = 4.58LYY81 pKa = 10.3DD82 pKa = 3.62RR83 pKa = 11.84SLRR86 pKa = 11.84RR87 pKa = 11.84EE88 pKa = 3.99MINVNKK94 pKa = 10.36AVTHH98 pKa = 6.42DD99 pKa = 4.11LSEE102 pKa = 4.39DD103 pKa = 3.59SYY105 pKa = 11.29MSNLTLHH112 pKa = 7.08ASCTYY117 pKa = 10.32FPDD120 pKa = 4.05SNSSGDD126 pKa = 3.61CSNISMGSDD135 pKa = 3.07VRR137 pKa = 11.84PPPKK141 pKa = 9.22TDD143 pKa = 3.05HH144 pKa = 6.96LEE146 pKa = 4.15LYY148 pKa = 10.26LPRR151 pKa = 11.84EE152 pKa = 4.17LRR154 pKa = 11.84STRR157 pKa = 11.84RR158 pKa = 11.84KK159 pKa = 9.47RR160 pKa = 11.84SSDD163 pKa = 3.08VVSYY167 pKa = 10.89MNTLLNLRR175 pKa = 11.84SAPDD179 pKa = 3.55EE180 pKa = 4.31YY181 pKa = 11.38SCSTLIPCIYY191 pKa = 9.9HH192 pKa = 5.45LTIGQFTYY200 pKa = 10.65LYY202 pKa = 9.98IVPQGDD208 pKa = 3.59ASCPSICEE216 pKa = 4.17DD217 pKa = 3.46GVSIGVLVASTTDD230 pKa = 3.4PSYY233 pKa = 11.13SWRR236 pKa = 11.84FGNQILACGTRR247 pKa = 11.84RR248 pKa = 11.84RR249 pKa = 11.84NSLLCSIFPRR259 pKa = 11.84QHH261 pKa = 5.48TCTCEE266 pKa = 3.8VLIQANNNLITPRR279 pKa = 11.84TIRR282 pKa = 11.84RR283 pKa = 11.84GKK285 pKa = 10.27RR286 pKa = 11.84GVTSGLRR293 pKa = 11.84RR294 pKa = 11.84AFNWLSKK301 pKa = 8.85VLKK304 pKa = 9.49WGPKK308 pKa = 9.68KK309 pKa = 10.08ILHH312 pKa = 5.9SFKK315 pKa = 9.85KK316 pKa = 10.18HH317 pKa = 4.36PWRR320 pKa = 11.84TVGGTVGFASGVYY333 pKa = 9.27TIYY336 pKa = 11.0EE337 pKa = 4.12LVEE340 pKa = 4.49LKK342 pKa = 10.66LSPPVPSHH350 pKa = 5.95EE351 pKa = 4.53EE352 pKa = 3.43IVSIVEE358 pKa = 4.22KK359 pKa = 10.32MRR361 pKa = 11.84QTDD364 pKa = 3.4QALVNVSDD372 pKa = 3.9SLYY375 pKa = 9.24VGIGAVHH382 pKa = 7.71DD383 pKa = 4.36DD384 pKa = 4.24LLNFRR389 pKa = 11.84QDD391 pKa = 3.36MKK393 pKa = 11.5DD394 pKa = 3.06SFHH397 pKa = 7.29QLGQWSDD404 pKa = 3.62QVTVIHH410 pKa = 6.52TLLSLCVSRR419 pKa = 11.84GQSGLSGLLRR429 pKa = 11.84GEE431 pKa = 4.55LPQTVISQEE440 pKa = 4.04SLQLFISTLLGKK452 pKa = 9.65EE453 pKa = 3.99LSSAVAMMPLPIRR466 pKa = 11.84LVNADD471 pKa = 5.25FISNMVWVSVRR482 pKa = 11.84VPKK485 pKa = 10.69KK486 pKa = 9.45GTTLLGRR493 pKa = 11.84VYY495 pKa = 10.64RR496 pKa = 11.84LDD498 pKa = 3.68GEE500 pKa = 4.58TRR502 pKa = 11.84TVNTPNDD509 pKa = 2.79ICYY512 pKa = 7.28TTRR515 pKa = 11.84GDD517 pKa = 3.42QYY519 pKa = 11.21YY520 pKa = 9.47FAPTSPDD527 pKa = 2.89IKK529 pKa = 9.12PTPSHH534 pKa = 7.21RR535 pKa = 11.84ITLEE539 pKa = 3.63SCTKK543 pKa = 10.29SGGILICPYY552 pKa = 11.07GSLTDD557 pKa = 3.51VTKK560 pKa = 8.73TTEE563 pKa = 3.59HH564 pKa = 7.37DD565 pKa = 3.38ICFSRR570 pKa = 11.84YY571 pKa = 9.3SSPKK575 pKa = 8.88RR576 pKa = 11.84SRR578 pKa = 11.84RR579 pKa = 11.84DD580 pKa = 3.26VNKK583 pKa = 10.62DD584 pKa = 2.64IITEE588 pKa = 4.08VLRR591 pKa = 11.84KK592 pKa = 8.35TSTFTEE598 pKa = 4.22IPLPSDD604 pKa = 3.5TPPSTFHH611 pKa = 6.62QFTEE615 pKa = 4.68RR616 pKa = 11.84YY617 pKa = 8.32KK618 pKa = 10.75VAISSLQEE626 pKa = 3.85SNKK629 pKa = 9.97RR630 pKa = 11.84LEE632 pKa = 4.64DD633 pKa = 3.05AWQEE637 pKa = 3.95LLEE640 pKa = 4.28KK641 pKa = 10.6HH642 pKa = 6.44SSLNLWSRR650 pKa = 11.84LHH652 pKa = 6.28LAWTSVFSFLTLLLVVKK669 pKa = 9.66VGWDD673 pKa = 2.78ILTTRR678 pKa = 11.84RR679 pKa = 11.84FRR681 pKa = 11.84QGLKK685 pKa = 8.82VTPDD689 pKa = 2.76HH690 pKa = 6.87HH691 pKa = 6.37EE692 pKa = 4.03AVSLRR697 pKa = 11.84EE698 pKa = 4.07RR699 pKa = 11.84GCDD702 pKa = 3.46TII704 pKa = 5.3

Molecular weight: 78.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3383

288

1926

845.8

93.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.124 ± 1.544

2.01 ± 0.279

4.877 ± 0.29

4.73 ± 0.194

3.636 ± 0.183

6.592 ± 0.523

2.424 ± 0.207

5.616 ± 0.52

4.523 ± 0.309

11.558 ± 0.72

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.069 ± 0.181

3.163 ± 0.129

5.823 ± 0.487

3.458 ± 0.328

5.616 ± 0.481

8.75 ± 1.111

6.71 ± 0.554

6.562 ± 0.404

1.567 ± 0.202

3.192 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski