Amino acid dipepetide frequency for Lactococcus phage 936 group phage Phi4.2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.015AlaAla: 1.015 ± 0.372
0.304AlaCys: 0.304 ± 0.19
3.044AlaAsp: 3.044 ± 0.615
4.567AlaGlu: 4.567 ± 0.741
4.364AlaPhe: 4.364 ± 0.764
4.871AlaGly: 4.871 ± 1.008
0.913AlaHis: 0.913 ± 0.319
5.581AlaIle: 5.581 ± 1.099
5.581AlaLys: 5.581 ± 0.884
6.292AlaLeu: 6.292 ± 1.125
2.233AlaMet: 2.233 ± 0.664
3.856AlaAsn: 3.856 ± 0.578
1.116AlaPro: 1.116 ± 0.372
2.131AlaGln: 2.131 ± 0.427
2.131AlaArg: 2.131 ± 0.424
2.943AlaSer: 2.943 ± 0.681
3.958AlaThr: 3.958 ± 0.83
4.364AlaVal: 4.364 ± 0.751
1.725AlaTrp: 1.725 ± 0.643
1.725AlaTyr: 1.725 ± 0.422
0.0AlaXaa: 0.0 ± 0.0
Cys
0.203CysAla: 0.203 ± 0.139
0.203CysCys: 0.203 ± 0.149
0.507CysAsp: 0.507 ± 0.259
0.609CysGlu: 0.609 ± 0.226
0.406CysPhe: 0.406 ± 0.245
0.609CysGly: 0.609 ± 0.266
0.203CysHis: 0.203 ± 0.162
0.203CysIle: 0.203 ± 0.162
1.218CysLys: 1.218 ± 0.409
0.406CysLeu: 0.406 ± 0.223
0.101CysMet: 0.101 ± 0.103
0.71CysAsn: 0.71 ± 0.256
0.203CysPro: 0.203 ± 0.126
0.203CysGln: 0.203 ± 0.135
0.71CysArg: 0.71 ± 0.267
0.304CysSer: 0.304 ± 0.174
0.203CysThr: 0.203 ± 0.13
0.71CysVal: 0.71 ± 0.255
0.203CysTrp: 0.203 ± 0.133
0.203CysTyr: 0.203 ± 0.166
0.0CysXaa: 0.0 ± 0.0
Asp
2.03AspAla: 2.03 ± 0.747
0.203AspCys: 0.203 ± 0.134
2.943AspAsp: 2.943 ± 0.659
3.653AspGlu: 3.653 ± 0.83
3.45AspPhe: 3.45 ± 0.468
3.958AspGly: 3.958 ± 0.76
1.218AspHis: 1.218 ± 0.339
3.552AspIle: 3.552 ± 0.51
4.465AspLys: 4.465 ± 0.614
6.19AspLeu: 6.19 ± 0.88
0.812AspMet: 0.812 ± 0.25
4.262AspAsn: 4.262 ± 0.688
1.218AspPro: 1.218 ± 0.375
1.218AspGln: 1.218 ± 0.413
1.421AspArg: 1.421 ± 0.415
3.247AspSer: 3.247 ± 0.519
3.45AspThr: 3.45 ± 0.672
2.943AspVal: 2.943 ± 0.774
0.812AspTrp: 0.812 ± 0.28
2.334AspTyr: 2.334 ± 0.368
0.0AspXaa: 0.0 ± 0.0
Glu
4.059GluAla: 4.059 ± 0.677
0.507GluCys: 0.507 ± 0.307
3.044GluAsp: 3.044 ± 0.511
3.45GluGlu: 3.45 ± 0.648
3.044GluPhe: 3.044 ± 0.449
2.436GluGly: 2.436 ± 0.454
1.015GluHis: 1.015 ± 0.343
5.48GluIle: 5.48 ± 0.691
5.784GluLys: 5.784 ± 1.155
8.829GluLeu: 8.829 ± 1.292
2.639GluMet: 2.639 ± 0.49
5.683GluAsn: 5.683 ± 0.773
1.522GluPro: 1.522 ± 0.392
4.364GluGln: 4.364 ± 0.748
2.639GluArg: 2.639 ± 0.525
3.755GluSer: 3.755 ± 0.533
4.871GluThr: 4.871 ± 0.763
3.45GluVal: 3.45 ± 0.531
1.015GluTrp: 1.015 ± 0.301
3.552GluTyr: 3.552 ± 0.653
0.0GluXaa: 0.0 ± 0.0
Phe
2.334PheAla: 2.334 ± 0.513
0.406PheCys: 0.406 ± 0.215
3.146PheAsp: 3.146 ± 0.517
3.146PheGlu: 3.146 ± 0.626
2.233PhePhe: 2.233 ± 0.667
2.233PheGly: 2.233 ± 0.556
0.609PheHis: 0.609 ± 0.252
4.161PheIle: 4.161 ± 0.72
3.856PheLys: 3.856 ± 0.654
2.639PheLeu: 2.639 ± 0.495
0.812PheMet: 0.812 ± 0.284
2.943PheAsn: 2.943 ± 0.536
1.015PhePro: 1.015 ± 0.342
1.421PheGln: 1.421 ± 0.474
1.218PheArg: 1.218 ± 0.295
3.755PheSer: 3.755 ± 0.727
3.755PheThr: 3.755 ± 0.596
2.131PheVal: 2.131 ± 0.446
0.203PheTrp: 0.203 ± 0.133
1.319PheTyr: 1.319 ± 0.321
0.0PheXaa: 0.0 ± 0.0
Gly
4.161GlyAla: 4.161 ± 1.166
0.406GlyCys: 0.406 ± 0.208
2.436GlyAsp: 2.436 ± 0.478
4.465GlyGlu: 4.465 ± 0.647
2.131GlyPhe: 2.131 ± 0.466
3.45GlyGly: 3.45 ± 0.708
0.71GlyHis: 0.71 ± 0.246
4.364GlyIle: 4.364 ± 1.247
6.698GlyLys: 6.698 ± 0.792
6.089GlyLeu: 6.089 ± 0.72
1.421GlyMet: 1.421 ± 0.516
3.856GlyAsn: 3.856 ± 0.547
0.203GlyPro: 0.203 ± 0.146
2.233GlyGln: 2.233 ± 0.445
2.639GlyArg: 2.639 ± 0.4
5.074GlySer: 5.074 ± 0.825
3.958GlyThr: 3.958 ± 0.811
4.668GlyVal: 4.668 ± 0.791
1.015GlyTrp: 1.015 ± 0.317
2.639GlyTyr: 2.639 ± 0.583
0.0GlyXaa: 0.0 ± 0.0
His
0.71HisAla: 0.71 ± 0.262
0.507HisCys: 0.507 ± 0.292
0.406HisAsp: 0.406 ± 0.192
0.913HisGlu: 0.913 ± 0.254
0.406HisPhe: 0.406 ± 0.283
1.522HisGly: 1.522 ± 0.368
0.101HisHis: 0.101 ± 0.091
1.319HisIle: 1.319 ± 0.343
1.015HisLys: 1.015 ± 0.35
0.812HisLeu: 0.812 ± 0.264
0.0HisMet: 0.0 ± 0.0
1.624HisAsn: 1.624 ± 0.515
0.304HisPro: 0.304 ± 0.157
0.304HisGln: 0.304 ± 0.183
0.71HisArg: 0.71 ± 0.294
0.101HisSer: 0.101 ± 0.093
0.913HisThr: 0.913 ± 0.296
0.609HisVal: 0.609 ± 0.257
0.101HisTrp: 0.101 ± 0.093
0.507HisTyr: 0.507 ± 0.202
0.0HisXaa: 0.0 ± 0.0
Ile
4.973IleAla: 4.973 ± 0.64
0.203IleCys: 0.203 ± 0.155
3.856IleAsp: 3.856 ± 0.573
6.596IleGlu: 6.596 ± 0.895
2.03IlePhe: 2.03 ± 0.487
3.044IleGly: 3.044 ± 0.567
0.913IleHis: 0.913 ± 0.245
3.958IleIle: 3.958 ± 0.615
7.104IleLys: 7.104 ± 0.834
4.77IleLeu: 4.77 ± 0.972
2.537IleMet: 2.537 ± 0.543
5.886IleAsn: 5.886 ± 0.733
1.319IlePro: 1.319 ± 0.333
2.436IleGln: 2.436 ± 0.528
2.03IleArg: 2.03 ± 0.395
3.653IleSer: 3.653 ± 0.939
5.886IleThr: 5.886 ± 0.788
4.668IleVal: 4.668 ± 0.666
1.116IleTrp: 1.116 ± 0.288
3.146IleTyr: 3.146 ± 0.585
0.0IleXaa: 0.0 ± 0.0
Lys
5.48LysAla: 5.48 ± 0.982
0.71LysCys: 0.71 ± 0.32
4.567LysAsp: 4.567 ± 0.683
7.611LysGlu: 7.611 ± 1.183
2.537LysPhe: 2.537 ± 0.495
6.393LysGly: 6.393 ± 0.855
1.116LysHis: 1.116 ± 0.325
5.379LysIle: 5.379 ± 0.767
8.524LysLys: 8.524 ± 0.91
7.51LysLeu: 7.51 ± 0.975
3.044LysMet: 3.044 ± 0.397
5.683LysAsn: 5.683 ± 0.932
2.131LysPro: 2.131 ± 0.611
3.45LysGln: 3.45 ± 0.653
3.958LysArg: 3.958 ± 0.69
4.871LysSer: 4.871 ± 0.793
5.379LysThr: 5.379 ± 0.812
6.292LysVal: 6.292 ± 0.661
1.218LysTrp: 1.218 ± 0.325
3.856LysTyr: 3.856 ± 0.645
0.0LysXaa: 0.0 ± 0.0
Leu
5.074LeuAla: 5.074 ± 0.557
0.304LeuCys: 0.304 ± 0.174
4.465LeuAsp: 4.465 ± 0.668
5.074LeuGlu: 5.074 ± 0.735
2.943LeuPhe: 2.943 ± 0.693
5.379LeuGly: 5.379 ± 0.903
1.522LeuHis: 1.522 ± 0.443
6.393LeuIle: 6.393 ± 0.997
8.017LeuLys: 8.017 ± 0.757
6.292LeuLeu: 6.292 ± 1.257
1.319LeuMet: 1.319 ± 0.376
5.48LeuAsn: 5.48 ± 0.893
2.334LeuPro: 2.334 ± 0.495
3.45LeuGln: 3.45 ± 0.547
2.537LeuArg: 2.537 ± 0.473
5.379LeuSer: 5.379 ± 0.883
6.495LeuThr: 6.495 ± 0.722
6.19LeuVal: 6.19 ± 0.831
1.725LeuTrp: 1.725 ± 0.415
3.958LeuTyr: 3.958 ± 0.588
0.0LeuXaa: 0.0 ± 0.0
Met
2.639MetAla: 2.639 ± 0.439
0.101MetCys: 0.101 ± 0.118
0.406MetAsp: 0.406 ± 0.188
1.928MetGlu: 1.928 ± 0.417
0.71MetPhe: 0.71 ± 0.282
1.015MetGly: 1.015 ± 0.302
0.304MetHis: 0.304 ± 0.171
2.334MetIle: 2.334 ± 0.46
2.943MetLys: 2.943 ± 0.538
0.913MetLeu: 0.913 ± 0.32
0.507MetMet: 0.507 ± 0.243
2.233MetAsn: 2.233 ± 0.497
0.507MetPro: 0.507 ± 0.221
1.928MetGln: 1.928 ± 0.447
0.812MetArg: 0.812 ± 0.331
1.827MetSer: 1.827 ± 0.529
1.522MetThr: 1.522 ± 0.413
0.913MetVal: 0.913 ± 0.242
0.203MetTrp: 0.203 ± 0.132
1.116MetTyr: 1.116 ± 0.35
0.0MetXaa: 0.0 ± 0.0
Asn
4.567AsnAla: 4.567 ± 0.858
0.304AsnCys: 0.304 ± 0.187
4.465AsnAsp: 4.465 ± 0.619
4.465AsnGlu: 4.465 ± 0.806
2.537AsnPhe: 2.537 ± 0.494
6.698AsnGly: 6.698 ± 0.86
0.913AsnHis: 0.913 ± 0.298
3.958AsnIle: 3.958 ± 0.79
5.48AsnLys: 5.48 ± 0.999
6.292AsnLeu: 6.292 ± 0.613
1.421AsnMet: 1.421 ± 0.427
3.958AsnAsn: 3.958 ± 0.703
2.334AsnPro: 2.334 ± 0.42
2.436AsnGln: 2.436 ± 0.478
2.233AsnArg: 2.233 ± 0.451
4.871AsnSer: 4.871 ± 0.606
4.871AsnThr: 4.871 ± 0.862
5.176AsnVal: 5.176 ± 0.898
1.116AsnTrp: 1.116 ± 0.386
2.131AsnTyr: 2.131 ± 0.546
0.0AsnXaa: 0.0 ± 0.0
Pro
2.334ProAla: 2.334 ± 0.56
0.101ProCys: 0.101 ± 0.106
1.827ProAsp: 1.827 ± 0.585
1.827ProGlu: 1.827 ± 0.482
1.218ProPhe: 1.218 ± 0.345
0.406ProGly: 0.406 ± 0.243
0.0ProHis: 0.0 ± 0.0
1.725ProIle: 1.725 ± 0.341
1.624ProLys: 1.624 ± 0.509
1.928ProLeu: 1.928 ± 0.393
0.609ProMet: 0.609 ± 0.243
2.537ProAsn: 2.537 ± 0.69
0.203ProPro: 0.203 ± 0.14
0.507ProGln: 0.507 ± 0.197
0.304ProArg: 0.304 ± 0.171
0.71ProSer: 0.71 ± 0.277
2.841ProThr: 2.841 ± 0.48
1.827ProVal: 1.827 ± 0.495
0.0ProTrp: 0.0 ± 0.0
0.406ProTyr: 0.406 ± 0.155
0.0ProXaa: 0.0 ± 0.0
Gln
3.653GlnAla: 3.653 ± 0.619
0.304GlnCys: 0.304 ± 0.177
1.725GlnAsp: 1.725 ± 0.444
2.74GlnGlu: 2.74 ± 0.547
1.319GlnPhe: 1.319 ± 0.347
3.044GlnGly: 3.044 ± 0.624
0.203GlnHis: 0.203 ± 0.147
1.319GlnIle: 1.319 ± 0.375
3.552GlnLys: 3.552 ± 0.659
3.755GlnLeu: 3.755 ± 0.489
0.913GlnMet: 0.913 ± 0.252
2.131GlnAsn: 2.131 ± 0.384
1.116GlnPro: 1.116 ± 0.384
2.131GlnGln: 2.131 ± 0.505
1.725GlnArg: 1.725 ± 0.342
2.233GlnSer: 2.233 ± 0.392
2.74GlnThr: 2.74 ± 0.424
2.131GlnVal: 2.131 ± 0.449
0.812GlnTrp: 0.812 ± 0.214
1.218GlnTyr: 1.218 ± 0.312
0.0GlnXaa: 0.0 ± 0.0
Arg
2.74ArgAla: 2.74 ± 0.606
0.812ArgCys: 0.812 ± 0.32
1.522ArgAsp: 1.522 ± 0.432
2.334ArgGlu: 2.334 ± 0.479
1.319ArgPhe: 1.319 ± 0.388
1.928ArgGly: 1.928 ± 0.449
0.507ArgHis: 0.507 ± 0.215
2.233ArgIle: 2.233 ± 0.516
3.45ArgLys: 3.45 ± 0.716
3.247ArgLeu: 3.247 ± 0.627
0.71ArgMet: 0.71 ± 0.325
2.131ArgAsn: 2.131 ± 0.472
0.913ArgPro: 0.913 ± 0.264
1.421ArgGln: 1.421 ± 0.361
1.725ArgArg: 1.725 ± 0.416
1.928ArgSer: 1.928 ± 0.473
1.218ArgThr: 1.218 ± 0.347
2.03ArgVal: 2.03 ± 0.352
0.304ArgTrp: 0.304 ± 0.181
1.725ArgTyr: 1.725 ± 0.421
0.0ArgXaa: 0.0 ± 0.0
Ser
4.262SerAla: 4.262 ± 0.881
0.609SerCys: 0.609 ± 0.335
3.247SerAsp: 3.247 ± 0.597
4.465SerGlu: 4.465 ± 0.768
3.044SerPhe: 3.044 ± 0.696
4.871SerGly: 4.871 ± 0.993
0.71SerHis: 0.71 ± 0.252
4.973SerIle: 4.973 ± 0.659
5.48SerLys: 5.48 ± 0.856
4.668SerLeu: 4.668 ± 0.675
1.522SerMet: 1.522 ± 0.368
4.262SerAsn: 4.262 ± 0.643
1.522SerPro: 1.522 ± 0.38
2.131SerGln: 2.131 ± 0.503
2.436SerArg: 2.436 ± 0.361
4.161SerSer: 4.161 ± 0.861
2.03SerThr: 2.03 ± 0.479
4.567SerVal: 4.567 ± 0.73
1.015SerTrp: 1.015 ± 0.36
2.131SerTyr: 2.131 ± 0.471
0.0SerXaa: 0.0 ± 0.0
Thr
5.683ThrAla: 5.683 ± 0.696
0.406ThrCys: 0.406 ± 0.189
3.958ThrAsp: 3.958 ± 0.834
5.277ThrGlu: 5.277 ± 0.501
3.146ThrPhe: 3.146 ± 0.81
4.465ThrGly: 4.465 ± 0.553
0.304ThrHis: 0.304 ± 0.17
4.871ThrIle: 4.871 ± 0.713
5.176ThrLys: 5.176 ± 0.528
5.886ThrLeu: 5.886 ± 0.791
1.319ThrMet: 1.319 ± 0.327
5.074ThrAsn: 5.074 ± 0.695
1.928ThrPro: 1.928 ± 0.35
2.639ThrGln: 2.639 ± 0.503
1.522ThrArg: 1.522 ± 0.411
4.465ThrSer: 4.465 ± 0.945
4.77ThrThr: 4.77 ± 0.816
5.379ThrVal: 5.379 ± 0.705
1.015ThrTrp: 1.015 ± 0.342
2.131ThrTyr: 2.131 ± 0.555
0.0ThrXaa: 0.0 ± 0.0
Val
4.364ValAla: 4.364 ± 0.886
0.609ValCys: 0.609 ± 0.253
4.364ValAsp: 4.364 ± 0.543
4.77ValGlu: 4.77 ± 0.566
3.044ValPhe: 3.044 ± 0.517
3.146ValGly: 3.146 ± 0.445
0.609ValHis: 0.609 ± 0.238
4.567ValIle: 4.567 ± 0.658
6.19ValLys: 6.19 ± 0.685
3.349ValLeu: 3.349 ± 0.542
1.421ValMet: 1.421 ± 0.317
2.841ValAsn: 2.841 ± 0.548
1.827ValPro: 1.827 ± 0.467
2.74ValGln: 2.74 ± 0.59
2.131ValArg: 2.131 ± 0.597
5.784ValSer: 5.784 ± 0.888
5.683ValThr: 5.683 ± 0.869
3.349ValVal: 3.349 ± 0.654
0.812ValTrp: 0.812 ± 0.394
2.74ValTyr: 2.74 ± 0.471
0.0ValXaa: 0.0 ± 0.0
Trp
0.609TrpAla: 0.609 ± 0.244
0.304TrpCys: 0.304 ± 0.19
1.015TrpAsp: 1.015 ± 0.521
0.71TrpGlu: 0.71 ± 0.237
1.015TrpPhe: 1.015 ± 0.337
1.015TrpGly: 1.015 ± 0.295
0.203TrpHis: 0.203 ± 0.142
0.812TrpIle: 0.812 ± 0.371
0.71TrpLys: 0.71 ± 0.246
1.319TrpLeu: 1.319 ± 0.398
0.304TrpMet: 0.304 ± 0.156
1.827TrpAsn: 1.827 ± 0.501
0.0TrpPro: 0.0 ± 0.0
0.609TrpGln: 0.609 ± 0.264
0.507TrpArg: 0.507 ± 0.308
1.116TrpSer: 1.116 ± 0.285
1.218TrpThr: 1.218 ± 0.426
0.406TrpVal: 0.406 ± 0.211
0.101TrpTrp: 0.101 ± 0.093
1.116TrpTyr: 1.116 ± 0.391
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.827TyrAla: 1.827 ± 0.44
0.812TyrCys: 0.812 ± 0.363
2.436TyrAsp: 2.436 ± 0.622
2.537TyrGlu: 2.537 ± 0.493
2.436TyrPhe: 2.436 ± 0.431
2.131TyrGly: 2.131 ± 0.481
0.812TyrHis: 0.812 ± 0.289
2.943TyrIle: 2.943 ± 0.614
2.943TyrLys: 2.943 ± 0.725
3.146TyrLeu: 3.146 ± 0.613
1.116TyrMet: 1.116 ± 0.403
3.247TyrAsn: 3.247 ± 0.547
1.218TyrPro: 1.218 ± 0.395
1.015TyrGln: 1.015 ± 0.308
0.812TyrArg: 0.812 ± 0.265
1.928TyrSer: 1.928 ± 0.489
3.653TyrThr: 3.653 ± 0.82
2.537TyrVal: 2.537 ± 0.575
0.406TyrTrp: 0.406 ± 0.191
1.928TyrTyr: 1.928 ± 0.503
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (9855 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski