Nosocomiicoccus massiliensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Staphylococcaceae; Nosocomiicoccus

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1652 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2J6NGG5|A0A2J6NGG5_9STAP tRNA uridine(34) hydroxylase OS=Nosocomiicoccus massiliensis OX=1232430 GN=trhO PE=3 SV=1
MM1 pKa = 7.45SYY3 pKa = 10.76SVNVYY8 pKa = 10.19GRR10 pKa = 11.84DD11 pKa = 3.8VICASCVNAPSSKK24 pKa = 9.7DD25 pKa = 3.08TYY27 pKa = 10.86QWLEE31 pKa = 3.59ALFSKK36 pKa = 10.35KK37 pKa = 10.64YY38 pKa = 8.37PDD40 pKa = 3.32ADD42 pKa = 3.02IRR44 pKa = 11.84VNYY47 pKa = 9.79IDD49 pKa = 4.47IDD51 pKa = 3.94RR52 pKa = 11.84DD53 pKa = 3.96EE54 pKa = 4.88NFSDD58 pKa = 3.87YY59 pKa = 11.26DD60 pKa = 3.66EE61 pKa = 4.73EE62 pKa = 6.56LIEE65 pKa = 5.07QINNDD70 pKa = 3.49EE71 pKa = 4.26LFYY74 pKa = 10.82PLVTINDD81 pKa = 3.56EE82 pKa = 4.49VIQDD86 pKa = 4.05GYY88 pKa = 11.2VQLKK92 pKa = 10.21PITNWFEE99 pKa = 4.31KK100 pKa = 10.86NGVEE104 pKa = 4.53

Molecular weight: 12.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2J6NK70|A0A2J6NK70_9STAP Amidohydrolase family protein (Fragment) OS=Nosocomiicoccus massiliensis OX=1232430 GN=CJ229_00700 PE=4 SV=1
MM1 pKa = 8.03PKK3 pKa = 8.95RR4 pKa = 11.84TYY6 pKa = 10.38QPNKK10 pKa = 8.21RR11 pKa = 11.84KK12 pKa = 9.62RR13 pKa = 11.84SKK15 pKa = 9.83VHH17 pKa = 5.77GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTKK26 pKa = 10.19NGRR29 pKa = 11.84KK30 pKa = 8.49VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.05GRR40 pKa = 11.84KK41 pKa = 8.7VLSAA45 pKa = 4.05

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1652

0

1652

486671

30

1420

294.6

33.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.793 ± 0.055

0.488 ± 0.016

6.308 ± 0.061

8.02 ± 0.085

4.594 ± 0.055

6.195 ± 0.055

2.121 ± 0.027

8.456 ± 0.072

7.195 ± 0.047

9.432 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.701 ± 0.03

5.25 ± 0.051

3.017 ± 0.03

2.903 ± 0.03

3.952 ± 0.052

5.903 ± 0.042

5.744 ± 0.051

7.33 ± 0.052

0.621 ± 0.018

3.978 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski