Salmonella phage VB_StyS_BS5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9LVZ7|A0A6B9LVZ7_9CAUD Neck whiskers protein OS=Salmonella phage VB_StyS_BS5 OX=2686071 PE=4 SV=1
MM1 pKa = 7.66ANMSYY6 pKa = 10.72CRR8 pKa = 11.84FRR10 pKa = 11.84NTEE13 pKa = 3.48QDD15 pKa = 3.71FNDD18 pKa = 3.9CVDD21 pKa = 4.39AIGNIEE27 pKa = 5.01SIDD30 pKa = 3.68EE31 pKa = 4.12LSNAEE36 pKa = 4.0RR37 pKa = 11.84KK38 pKa = 9.38SAEE41 pKa = 3.73RR42 pKa = 11.84LYY44 pKa = 11.18YY45 pKa = 10.32LANEE49 pKa = 4.16YY50 pKa = 9.7IAYY53 pKa = 9.95YY54 pKa = 9.75EE55 pKa = 3.92QLLEE59 pKa = 4.22EE60 pKa = 4.58SGEE63 pKa = 4.05

Molecular weight: 7.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9LQT2|A0A6B9LQT2_9CAUD Uncharacterized protein OS=Salmonella phage VB_StyS_BS5 OX=2686071 PE=4 SV=1
MM1 pKa = 7.72GSTNGPSRR9 pKa = 11.84SRR11 pKa = 11.84ATGNTKK17 pKa = 9.72TGGKK21 pKa = 7.78TGAVKK26 pKa = 10.54PNGSTRR32 pKa = 11.84SPSRR36 pKa = 11.84GKK38 pKa = 10.36KK39 pKa = 9.35

Molecular weight: 3.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

14390

31

1097

173.4

19.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.652 ± 0.709

1.598 ± 0.217

6.303 ± 0.312

6.407 ± 0.319

3.621 ± 0.207

7.387 ± 0.318

1.897 ± 0.246

6.852 ± 0.251

6.511 ± 0.392

7.06 ± 0.219

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.127 ± 0.207

4.587 ± 0.265

3.148 ± 0.214

3.815 ± 0.37

5.351 ± 0.232

6.449 ± 0.315

5.462 ± 0.364

6.539 ± 0.209

1.529 ± 0.156

3.704 ± 0.238
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski