Torulaspora delbrueckii (strain ATCC 10662 / CBS 1146 / NBRC 0425 / NCYC 2629 / NRRL Y-866) (Yeast) (Candida colliculosa)
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4966 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G8ZTG6|G8ZTG6_TORDC UBC core domain-containing protein OS=Torulaspora delbrueckii (strain ATCC 10662 / CBS 1146 / NBRC 0425 / NCYC 2629 / NRRL Y-866) OX=1076872 GN=TDEL0D03260 PE=3 SV=1
MM1 pKa = 7.36 NPITVLFHH9 pKa = 6.81 LALAYY14 pKa = 9.72 TSFTGIDD21 pKa = 3.57 LADD24 pKa = 3.81 ASAVDD29 pKa = 3.89 VEE31 pKa = 4.77 VVTDD35 pKa = 3.7 QVSKK39 pKa = 9.98 VTIDD43 pKa = 3.65 LNQLMDD49 pKa = 3.56 QAGTNSEE56 pKa = 4.27 SSDD59 pKa = 3.13 ASAKK63 pKa = 9.0 VLSYY67 pKa = 10.46 IVNSDD72 pKa = 3.61 SPVNSVVEE80 pKa = 4.6 AYY82 pKa = 9.89 DD83 pKa = 3.52 AAIDD87 pKa = 4.14 AGDD90 pKa = 3.41 ISLATVYY97 pKa = 8.87 ATKK100 pKa = 10.86 LMGLIEE106 pKa = 4.49 SVDD109 pKa = 3.78 DD110 pKa = 4.92 LISDD114 pKa = 4.19 IYY116 pKa = 10.9 AAPDD120 pKa = 3.7 YY121 pKa = 10.43 YY122 pKa = 11.42 SEE124 pKa = 5.32 IIPKK128 pKa = 9.71 QLITGAASQLAKK140 pKa = 10.07 RR141 pKa = 11.84 DD142 pKa = 3.69 QVDD145 pKa = 3.23 NDD147 pKa = 3.23 ATRR150 pKa = 11.84 VLDD153 pKa = 3.89 ALLNIMGTNATAQSNQLDD171 pKa = 4.16 DD172 pKa = 3.88 GTTGLVRR179 pKa = 11.84 RR180 pKa = 11.84 DD181 pKa = 2.98 QCGNYY186 pKa = 10.01 NVDD189 pKa = 3.25 TSNVVALSVFNSMRR203 pKa = 11.84 GQIDD207 pKa = 3.61 GNMWYY212 pKa = 9.78 PNDD215 pKa = 3.54 PRR217 pKa = 11.84 NFCRR221 pKa = 11.84 WYY223 pKa = 9.92 EE224 pKa = 3.94 DD225 pKa = 3.54 LRR227 pKa = 11.84 GCISWSTDD235 pKa = 3.04 VGGMPGNALQSWAADD250 pKa = 3.5 MSNCIPDD257 pKa = 3.83 AHH259 pKa = 6.5 PKK261 pKa = 9.59 SLKK264 pKa = 10.34 SLFAFGVSQGSTYY277 pKa = 10.66 VCGSGRR283 pKa = 11.84 PDD285 pKa = 2.95 GCC287 pKa = 4.42
Molecular weight: 30.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.923
IPC_protein 3.961
Toseland 3.719
ProMoST 4.139
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.948
Rodwell 3.77
Grimsley 3.617
Solomon 3.961
Lehninger 3.923
Nozaki 4.075
DTASelect 4.406
Thurlkill 3.783
EMBOSS 3.948
Sillero 4.088
Patrickios 1.952
IPC_peptide 3.961
IPC2_peptide 4.05
IPC2.peptide.svr19 3.957
Protein with the highest isoelectric point:
>tr|G8ZZK0|G8ZZK0_TORDC Topoisomerase I damage affected protein 2 OS=Torulaspora delbrueckii (strain ATCC 10662 / CBS 1146 / NBRC 0425 / NCYC 2629 / NRRL Y-866) OX=1076872 GN=TDEL0H01850 PE=3 SV=1
MM1 pKa = 7.1 RR2 pKa = 11.84 AKK4 pKa = 9.09 WRR6 pKa = 11.84 KK7 pKa = 8.87 KK8 pKa = 8.73 RR9 pKa = 11.84 VRR11 pKa = 11.84 RR12 pKa = 11.84 LKK14 pKa = 9.71 RR15 pKa = 11.84 KK16 pKa = 8.27 RR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 9.19 VRR21 pKa = 11.84 ARR23 pKa = 11.84 SKK25 pKa = 10.98
Molecular weight: 3.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4966
0
4966
2412323
25
4909
485.8
54.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.124 ± 0.029
1.297 ± 0.012
5.973 ± 0.022
6.859 ± 0.032
4.299 ± 0.023
5.387 ± 0.026
2.148 ± 0.012
5.874 ± 0.023
6.889 ± 0.031
9.748 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.129 ± 0.013
5.066 ± 0.02
4.408 ± 0.026
4.182 ± 0.026
4.93 ± 0.021
8.647 ± 0.037
5.551 ± 0.029
6.12 ± 0.023
1.084 ± 0.01
3.282 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here