White clover mosaic virus (strain M) (WCMV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Potexvirus; White clover mosaic virus

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P09502|CAPSD_WCMVM Coat protein OS=White clover mosaic virus (strain M) OX=12189 PE=3 SV=1
MM1 pKa = 8.19DD2 pKa = 4.49FTTLIIIGVYY12 pKa = 9.47LLVFIVYY19 pKa = 8.66FAKK22 pKa = 10.56INTSVCTISISGASIEE38 pKa = 5.5ISGCDD43 pKa = 3.33NPTLFEE49 pKa = 4.61ILPKK53 pKa = 10.34LRR55 pKa = 11.84PFNHH59 pKa = 6.28GLSLPSNN66 pKa = 4.05

Molecular weight: 7.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P09501|TGB3_WCMVM Movement protein TGBp3 OS=White clover mosaic virus (strain M) OX=12189 GN=ORF4 PE=3 SV=1
MM1 pKa = 7.91PLTPPPNPQKK11 pKa = 9.65TYY13 pKa = 10.77QIAILALGLVLLAFVLISDD32 pKa = 4.73HH33 pKa = 5.99SPKK36 pKa = 10.84VGDD39 pKa = 4.33HH40 pKa = 5.93LHH42 pKa = 6.12NLPFGGEE49 pKa = 4.17YY50 pKa = 10.27KK51 pKa = 10.78DD52 pKa = 3.51GTKK55 pKa = 10.23SIKK58 pKa = 10.17YY59 pKa = 6.91FQRR62 pKa = 11.84PNQHH66 pKa = 6.21SLSKK70 pKa = 9.83TLAKK74 pKa = 10.12SHH76 pKa = 5.86NTTIFLLILGLIVTLHH92 pKa = 5.92GLHH95 pKa = 6.15YY96 pKa = 10.35FNNNRR101 pKa = 11.84RR102 pKa = 11.84VSSSLHH108 pKa = 6.54CVLCQNKK115 pKa = 9.2HH116 pKa = 4.36

Molecular weight: 12.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1902

66

1294

380.4

42.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.256 ± 1.439

1.577 ± 0.282

5.31 ± 0.502

4.995 ± 1.068

5.1 ± 0.414

4.206 ± 0.563

3.575 ± 0.451

7.256 ± 0.794

6.467 ± 0.644

10.515 ± 1.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.682 ± 0.386

5.152 ± 0.838

6.046 ± 0.473

3.943 ± 0.541

3.575 ± 0.419

6.782 ± 0.773

7.676 ± 0.487

4.311 ± 0.258

1.157 ± 0.373

3.417 ± 0.357
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski