Porcine associated porprismacovirus 5

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Porprismacovirus

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A076VEN2|A0A076VEN2_9VIRU Cap protein OS=Porcine associated porprismacovirus 5 OX=2170121 PE=4 SV=1
MM1 pKa = 6.78ATNYY5 pKa = 7.69VTAKK9 pKa = 9.39YY10 pKa = 10.33QEE12 pKa = 4.47IYY14 pKa = 11.05DD15 pKa = 4.59LGTQAGKK22 pKa = 7.12TTILGIHH29 pKa = 6.11TPDD32 pKa = 3.53GGNVYY37 pKa = 11.15NMLSGFFDD45 pKa = 3.12QFRR48 pKa = 11.84KK49 pKa = 10.1YY50 pKa = 9.98RR51 pKa = 11.84YY52 pKa = 8.46KK53 pKa = 9.8GCSVSMVPAAQLPADD68 pKa = 4.06PLQVSFEE75 pKa = 4.28AGALTCDD82 pKa = 4.45PRR84 pKa = 11.84DD85 pKa = 3.67LLNPILFHH93 pKa = 6.36GCHH96 pKa = 5.99GEE98 pKa = 3.96EE99 pKa = 4.42LNEE102 pKa = 4.19ILDD105 pKa = 3.73SVYY108 pKa = 11.28YY109 pKa = 10.58KK110 pKa = 10.04GTSPSSVRR118 pKa = 11.84EE119 pKa = 4.05LQVNTQVSEE128 pKa = 3.91ASYY131 pKa = 10.76YY132 pKa = 10.88AALSDD137 pKa = 3.46PSFRR141 pKa = 11.84KK142 pKa = 9.93FGIQQGAKK150 pKa = 8.93VSLRR154 pKa = 11.84PMVHH158 pKa = 6.81PLAVTQPIEE167 pKa = 4.26HH168 pKa = 6.98SDD170 pKa = 3.01SKK172 pKa = 11.41YY173 pKa = 9.82YY174 pKa = 10.81YY175 pKa = 9.32SYY177 pKa = 10.8PSGGTTVTGSVDD189 pKa = 3.36GVEE192 pKa = 4.38SGDD195 pKa = 3.93LGMVQNGTNFTRR207 pKa = 11.84FGLQDD212 pKa = 3.13ACDD215 pKa = 3.63AVEE218 pKa = 4.1LHH220 pKa = 6.34VSGAGIPSGSVYY232 pKa = 10.59VSPTKK237 pKa = 10.41TIFSNGLKK245 pKa = 9.64PLSWLPTWHH254 pKa = 6.3FVPYY258 pKa = 10.24AGEE261 pKa = 3.99NMLTVPYY268 pKa = 9.78QNRR271 pKa = 11.84VSDD274 pKa = 3.59MSIEE278 pKa = 4.29TTRR281 pKa = 11.84LPLLYY286 pKa = 9.15MGLFILPPSYY296 pKa = 9.24TQEE299 pKa = 3.69MYY301 pKa = 10.28FRR303 pKa = 11.84LIITHH308 pKa = 5.6YY309 pKa = 11.04FEE311 pKa = 6.03FKK313 pKa = 10.81DD314 pKa = 4.68FSTCLTRR321 pKa = 11.84HH322 pKa = 5.99SVDD325 pKa = 3.2QLNPDD330 pKa = 3.21RR331 pKa = 11.84KK332 pKa = 10.39VYY334 pKa = 10.5TEE336 pKa = 4.8TIPEE340 pKa = 4.16PTSGSSASSLTVVDD354 pKa = 5.48GSAVQTSEE362 pKa = 3.93GVYY365 pKa = 10.53

Molecular weight: 40.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A076VEN2|A0A076VEN2_9VIRU Cap protein OS=Porcine associated porprismacovirus 5 OX=2170121 PE=4 SV=1
MM1 pKa = 6.82QAYY4 pKa = 9.2MMTIPRR10 pKa = 11.84TVSKK14 pKa = 10.35RR15 pKa = 11.84ALRR18 pKa = 11.84IMIDD22 pKa = 3.53VNDD25 pKa = 3.98CKK27 pKa = 10.84KK28 pKa = 10.0WIIGKK33 pKa = 9.24EE34 pKa = 3.97EE35 pKa = 3.89GKK37 pKa = 10.27NGYY40 pKa = 8.24KK41 pKa = 9.71HH42 pKa = 4.18WQIRR46 pKa = 11.84IEE48 pKa = 4.05TSNDD52 pKa = 3.05SFFEE56 pKa = 3.95WMQDD60 pKa = 3.65HH61 pKa = 7.09IPTAHH66 pKa = 7.41IEE68 pKa = 4.07RR69 pKa = 11.84TEE71 pKa = 4.27CGVDD75 pKa = 2.94ACRR78 pKa = 11.84YY79 pKa = 5.85EE80 pKa = 4.53AKK82 pKa = 10.15EE83 pKa = 4.03GQYY86 pKa = 11.57VMYY89 pKa = 10.37SDD91 pKa = 5.32RR92 pKa = 11.84PQNLMQRR99 pKa = 11.84YY100 pKa = 9.44GEE102 pKa = 3.85FRR104 pKa = 11.84PNQKK108 pKa = 9.86HH109 pKa = 6.04ALQALQQSNDD119 pKa = 3.33RR120 pKa = 11.84EE121 pKa = 4.4VVVWYY126 pKa = 10.26DD127 pKa = 3.15EE128 pKa = 4.19TGNVGKK134 pKa = 10.24SWFTGALWEE143 pKa = 4.29RR144 pKa = 11.84KK145 pKa = 8.78LAYY148 pKa = 7.69VTPPTIDD155 pKa = 3.16TVKK158 pKa = 11.11GLIQWIASCYY168 pKa = 9.96IEE170 pKa = 5.69DD171 pKa = 3.38GWRR174 pKa = 11.84PYY176 pKa = 10.39IIIDD180 pKa = 4.57IPRR183 pKa = 11.84SWKK186 pKa = 9.67WSEE189 pKa = 3.8QLYY192 pKa = 10.74SAIEE196 pKa = 4.22SIKK199 pKa = 11.0DD200 pKa = 3.09GLIYY204 pKa = 9.09DD205 pKa = 4.14TRR207 pKa = 11.84YY208 pKa = 9.36HH209 pKa = 7.23SSMMNIRR216 pKa = 11.84GVKK219 pKa = 9.02VLVMTNTMPKK229 pKa = 9.86LDD231 pKa = 4.6KK232 pKa = 10.62LSKK235 pKa = 10.59DD236 pKa = 2.97RR237 pKa = 11.84WCIRR241 pKa = 11.84TFF243 pKa = 3.22

Molecular weight: 28.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

608

243

365

304.0

34.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.934 ± 0.236

1.645 ± 0.24

5.428 ± 0.432

5.263 ± 0.528

3.454 ± 0.81

7.072 ± 1.0

2.303 ± 0.142

5.921 ± 1.818

4.77 ± 1.292

7.072 ± 1.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.289 ± 0.718

3.454 ± 0.145

5.428 ± 1.0

4.605 ± 0.193

4.77 ± 1.292

8.388 ± 1.524

7.237 ± 0.856

6.908 ± 0.904

2.138 ± 1.386

5.921 ± 0.332
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski