Enterococcus phage vB_EfaP_IME195

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Rountreeviridae; Sarlesvirinae; Copernicusvirus; Enterococcus virus IME195

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S2MY45|A0A0S2MY45_9CAUD DNA packaging protein OS=Enterococcus phage vB_EfaP_IME195 OX=1747288 PE=4 SV=1
MM1 pKa = 7.88PSYY4 pKa = 9.55KK5 pKa = 10.19TYY7 pKa = 8.3TTEE10 pKa = 3.54QYY12 pKa = 10.69QAFLSQPFGYY22 pKa = 10.5DD23 pKa = 3.0FGVSDD28 pKa = 3.83EE29 pKa = 5.35TIAQWFMIQPGARR42 pKa = 11.84PVINSYY48 pKa = 10.97GVTKK52 pKa = 10.88ANLLSDD58 pKa = 4.88YY59 pKa = 10.56IPKK62 pKa = 10.35LKK64 pKa = 10.61QEE66 pKa = 4.42FGGSLVFLMTTVSEE80 pKa = 4.46GGGAGNWVNHH90 pKa = 5.17YY91 pKa = 10.31MSDD94 pKa = 3.36TSDD97 pKa = 3.03NGMGCMIDD105 pKa = 4.01DD106 pKa = 3.51IAYY109 pKa = 9.67IKK111 pKa = 8.15TTFDD115 pKa = 2.69RR116 pKa = 11.84HH117 pKa = 5.38FPPAISAPEE126 pKa = 3.73VGGAYY131 pKa = 9.33TEE133 pKa = 4.56DD134 pKa = 3.83EE135 pKa = 4.7EE136 pKa = 4.86GLTMKK141 pKa = 10.33VYY143 pKa = 10.83NAVPDD148 pKa = 4.24GSIGSYY154 pKa = 9.63FIPSTMAGNAWIFGSQWCLANQGAAPPAVYY184 pKa = 10.15FGNPYY189 pKa = 10.29DD190 pKa = 3.84QLIDD194 pKa = 3.81IIKK197 pKa = 10.65SFGADD202 pKa = 3.34PFKK205 pKa = 10.91EE206 pKa = 4.26GSTAKK211 pKa = 10.24PNPDD215 pKa = 3.49TPKK218 pKa = 10.8GDD220 pKa = 3.97PNEE223 pKa = 4.66SGNKK227 pKa = 8.93PKK229 pKa = 10.43PKK231 pKa = 9.78PDD233 pKa = 3.01IQKK236 pKa = 10.85AIDD239 pKa = 4.27KK240 pKa = 10.73ILDD243 pKa = 4.43EE244 pKa = 4.75INKK247 pKa = 9.98ALDD250 pKa = 3.31NQTIQGSPLLSYY262 pKa = 11.17NDD264 pKa = 3.78DD265 pKa = 3.49VTIEE269 pKa = 4.01RR270 pKa = 11.84TFNNSYY276 pKa = 10.08KK277 pKa = 10.11ISYY280 pKa = 8.6TSSFKK285 pKa = 10.9KK286 pKa = 10.36KK287 pKa = 10.6LSDD290 pKa = 3.39MLNASDD296 pKa = 5.62LGLITDD302 pKa = 5.61DD303 pKa = 4.39GAQTNPPKK311 pKa = 10.21EE312 pKa = 4.25DD313 pKa = 3.93PKK315 pKa = 10.91PPSIDD320 pKa = 3.02AGKK323 pKa = 10.28EE324 pKa = 3.85SKK326 pKa = 9.13TMKK329 pKa = 10.34KK330 pKa = 9.98IYY332 pKa = 9.75DD333 pKa = 3.71WCNEE337 pKa = 3.98NQGQAFDD344 pKa = 3.54TDD346 pKa = 3.98GYY348 pKa = 11.55YY349 pKa = 10.79GAQCVDD355 pKa = 5.07LISWLNTKK363 pKa = 9.72VFGLGLDD370 pKa = 3.55TSGDD374 pKa = 3.6YY375 pKa = 11.17AKK377 pKa = 10.76NIWNNPVPAGWYY389 pKa = 9.22KK390 pKa = 11.3VNGNPNDD397 pKa = 3.97DD398 pKa = 3.77NASRR402 pKa = 11.84EE403 pKa = 3.99IWNTLPNGAIVWWTNSGAGHH423 pKa = 6.09VGVKK427 pKa = 10.45AGDD430 pKa = 3.55FAQTLQQNWTSHH442 pKa = 5.43GLGGPIVLADD452 pKa = 3.69CASWMVSSNSGFLGAWVTDD471 pKa = 3.66NN472 pKa = 4.3

Molecular weight: 51.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S2MY57|A0A0S2MY57_9CAUD Minor capsid protein OS=Enterococcus phage vB_EfaP_IME195 OX=1747288 PE=4 SV=1
MM1 pKa = 7.86KK2 pKa = 10.38SKK4 pKa = 10.88QKK6 pKa = 10.75LKK8 pKa = 11.28YY9 pKa = 9.85KK10 pKa = 9.76FTFNKK15 pKa = 9.52QLYY18 pKa = 10.25GDD20 pKa = 4.08IADD23 pKa = 4.04EE24 pKa = 4.46AQAKK28 pKa = 9.93LDD30 pKa = 3.59RR31 pKa = 11.84LHH33 pKa = 7.14FVTQQRR39 pKa = 11.84GDD41 pKa = 3.54RR42 pKa = 11.84FPKK45 pKa = 9.88MGAISLNEE53 pKa = 3.66AMNLVEE59 pKa = 4.62GTWSRR64 pKa = 11.84ALDD67 pKa = 3.4TLINYY72 pKa = 9.38FSMSLSEE79 pKa = 4.26VNQHH83 pKa = 6.06KK84 pKa = 10.09LASTKK89 pKa = 10.52RR90 pKa = 11.84FIGDD94 pKa = 4.47KK95 pKa = 9.71IAQNATSIPNLMLVVLEE112 pKa = 4.16YY113 pKa = 10.56AQKK116 pKa = 10.88FEE118 pKa = 4.42SQTKK122 pKa = 8.77KK123 pKa = 10.73DD124 pKa = 3.22KK125 pKa = 10.68NRR127 pKa = 11.84RR128 pKa = 11.84FSIIAYY134 pKa = 8.74QEE136 pKa = 4.53GYY138 pKa = 10.61NGG140 pKa = 3.43

Molecular weight: 16.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27

0

27

5841

52

782

216.3

24.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.102 ± 0.315

0.856 ± 0.139

6.215 ± 0.319

7.601 ± 0.66

4.708 ± 0.415

6.026 ± 0.554

1.763 ± 0.166

6.54 ± 0.179

8.047 ± 0.403

8.303 ± 0.439

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.739 ± 0.177

7.122 ± 0.415

3.475 ± 0.369

3.287 ± 0.219

3.356 ± 0.317

5.53 ± 0.249

6.865 ± 0.308

5.855 ± 0.25

1.335 ± 0.207

5.273 ± 0.295
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski