Cellulomonas sp. PSBB021
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3224 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A222XBX1|A0A222XBX1_9CELL Peptidase_M23 domain-containing protein OS=Cellulomonas sp. PSBB021 OX=2003551 GN=CBP52_10650 PE=4 SV=1
MM1 pKa = 7.46 LPSKK5 pKa = 9.78 SLRR8 pKa = 11.84 TAGLASGALAALLALSACSSSSSATDD34 pKa = 3.56 DD35 pKa = 3.39 ATTAAPAAGAYY46 pKa = 9.5 QSLADD51 pKa = 4.08 SSACVDD57 pKa = 4.0 LRR59 pKa = 11.84 EE60 pKa = 4.32 TNPDD64 pKa = 3.12 LVGTTKK70 pKa = 10.46 TNALNPHH77 pKa = 5.19 TPGYY81 pKa = 10.45 EE82 pKa = 4.11 VIDD85 pKa = 4.28 PEE87 pKa = 5.76 NPDD90 pKa = 3.17 QYY92 pKa = 11.23 IGFDD96 pKa = 3.3 IDD98 pKa = 3.26 LGEE101 pKa = 5.45 AIGACLGFEE110 pKa = 4.0 VDD112 pKa = 4.08 YY113 pKa = 11.1 IPVGFAEE120 pKa = 5.9 LIPTVASGQADD131 pKa = 3.87 WIVSNLYY138 pKa = 9.01 ATEE141 pKa = 3.8 EE142 pKa = 4.06 RR143 pKa = 11.84 AQGGVDD149 pKa = 4.49 FISYY153 pKa = 10.73 SKK155 pKa = 11.02 VFDD158 pKa = 5.15 GILVTTGNPKK168 pKa = 10.69 GITGIDD174 pKa = 3.32 TSVCGTTVALNKK186 pKa = 10.51 GYY188 pKa = 11.09 VEE190 pKa = 4.44 VPLVEE195 pKa = 4.82 AMGPEE200 pKa = 4.52 CEE202 pKa = 4.5 KK203 pKa = 11.07 AGLPAPTVSLFDD215 pKa = 3.8 SSADD219 pKa = 3.46 CVQAILAGRR228 pKa = 11.84 ADD230 pKa = 4.08 AYY232 pKa = 10.87 MNDD235 pKa = 2.91 INTVKK240 pKa = 10.58 GYY242 pKa = 9.95 IAEE245 pKa = 4.74 HH246 pKa = 6.8 PDD248 pKa = 3.49 EE249 pKa = 5.35 LDD251 pKa = 3.39 SAEE254 pKa = 4.23 TVMLDD259 pKa = 3.25 YY260 pKa = 11.0 EE261 pKa = 4.21 IGIGVLQGDD270 pKa = 3.91 HH271 pKa = 7.04 AFRR274 pKa = 11.84 DD275 pKa = 4.01 AVQAALLEE283 pKa = 4.33 IQNSGLQAEE292 pKa = 5.33 LATKK296 pKa = 9.69 WKK298 pKa = 10.36 LDD300 pKa = 3.73 EE301 pKa = 4.38 NAVAEE306 pKa = 4.16 PSILSVGG313 pKa = 3.5
Molecular weight: 32.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.723
IPC2_protein 3.897
IPC_protein 3.897
Toseland 3.694
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.719
Grimsley 3.592
Solomon 3.872
Lehninger 3.821
Nozaki 3.986
DTASelect 4.202
Thurlkill 3.732
EMBOSS 3.808
Sillero 4.012
Patrickios 1.24
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.892
Protein with the highest isoelectric point:
>tr|A0A222XBS4|A0A222XBS4_9CELL TetR family transcriptional regulator OS=Cellulomonas sp. PSBB021 OX=2003551 GN=CBP52_12300 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3224
0
3224
1072490
32
1984
332.7
35.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.477 ± 0.068
0.613 ± 0.01
6.584 ± 0.033
5.187 ± 0.041
2.447 ± 0.025
9.226 ± 0.041
2.162 ± 0.024
2.617 ± 0.034
1.478 ± 0.029
10.123 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.445 ± 0.018
1.458 ± 0.025
5.973 ± 0.036
2.693 ± 0.024
8.205 ± 0.061
4.996 ± 0.038
6.503 ± 0.051
10.39 ± 0.045
1.599 ± 0.019
1.824 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here