archaeon HR01

Taxonomy: cellular organisms; Archaea; unclassified Archaea

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1916 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5UZA5|A0A2H5UZA5_9ARCH Uncharacterized protein OS=archaeon HR01 OX=2035437 GN=HRbin01_01472 PE=4 SV=1
MM1 pKa = 6.98YY2 pKa = 9.63HH3 pKa = 6.72HH4 pKa = 7.45EE5 pKa = 4.14YY6 pKa = 11.01LRR8 pKa = 11.84MEE10 pKa = 4.19EE11 pKa = 4.04EE12 pKa = 4.24EE13 pKa = 4.07EE14 pKa = 4.07FEE16 pKa = 4.64EE17 pKa = 4.61EE18 pKa = 4.55FEE20 pKa = 4.37EE21 pKa = 4.6EE22 pKa = 4.12DD23 pKa = 3.74FEE25 pKa = 4.62EE26 pKa = 4.89EE27 pKa = 3.89EE28 pKa = 4.28FEE30 pKa = 4.26EE31 pKa = 4.59EE32 pKa = 4.22EE33 pKa = 4.27EE34 pKa = 4.63EE35 pKa = 4.45EE36 pKa = 4.31FF37 pKa = 5.14

Molecular weight: 4.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5UZ17|A0A2H5UZ17_9ARCH ABC2_membrane domain-containing protein OS=archaeon HR01 OX=2035437 GN=HRbin01_01379 PE=4 SV=1
MM1 pKa = 7.78TIRR4 pKa = 11.84GLEE7 pKa = 4.11WIALALALLLIAILVLRR24 pKa = 11.84RR25 pKa = 11.84VRR27 pKa = 11.84RR28 pKa = 11.84VRR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84SRR34 pKa = 11.84RR35 pKa = 11.84FRR37 pKa = 11.84IGG39 pKa = 2.82

Molecular weight: 4.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1916

0

1916

533512

29

2869

278.5

30.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.243 ± 0.058

0.876 ± 0.019

4.668 ± 0.039

6.962 ± 0.053

3.596 ± 0.036

8.152 ± 0.049

1.756 ± 0.02

6.505 ± 0.046

4.676 ± 0.046

10.614 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.533 ± 0.025

2.679 ± 0.029

4.666 ± 0.037

2.22 ± 0.029

7.175 ± 0.051

6.342 ± 0.041

4.942 ± 0.05

8.646 ± 0.051

1.169 ± 0.025

3.581 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski