Amino acid dipepetide frequency for Micromonospora endophytica (Xie et al. 2001) Li et al. 2019

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.483AlaAla: 22.483 ± 0.176
0.941AlaCys: 0.941 ± 0.026
8.712AlaAsp: 8.712 ± 0.087
8.645AlaGlu: 8.645 ± 0.085
3.187AlaPhe: 3.187 ± 0.043
14.152AlaGly: 14.152 ± 0.101
2.572AlaHis: 2.572 ± 0.038
4.046AlaIle: 4.046 ± 0.05
2.167AlaLys: 2.167 ± 0.046
14.232AlaLeu: 14.232 ± 0.124
2.357AlaMet: 2.357 ± 0.037
2.253AlaAsn: 2.253 ± 0.043
6.797AlaPro: 6.797 ± 0.066
3.892AlaGln: 3.892 ± 0.052
10.303AlaArg: 10.303 ± 0.093
5.536AlaSer: 5.536 ± 0.061
8.196AlaThr: 8.196 ± 0.077
12.523AlaVal: 12.523 ± 0.092
1.91AlaTrp: 1.91 ± 0.036
2.83AlaTyr: 2.83 ± 0.04
0.0AlaXaa: 0.0 ± 0.0
Cys
1.001CysAla: 1.001 ± 0.023
0.097CysCys: 0.097 ± 0.007
0.476CysAsp: 0.476 ± 0.016
0.322CysGlu: 0.322 ± 0.014
0.227CysPhe: 0.227 ± 0.011
0.929CysGly: 0.929 ± 0.025
0.206CysHis: 0.206 ± 0.011
0.153CysIle: 0.153 ± 0.009
0.077CysLys: 0.077 ± 0.006
0.706CysLeu: 0.706 ± 0.019
0.101CysMet: 0.101 ± 0.006
0.14CysAsn: 0.14 ± 0.009
0.491CysPro: 0.491 ± 0.018
0.201CysGln: 0.201 ± 0.011
0.641CysArg: 0.641 ± 0.021
0.357CysSer: 0.357 ± 0.014
0.438CysThr: 0.438 ± 0.016
0.663CysVal: 0.663 ± 0.02
0.139CysTrp: 0.139 ± 0.009
0.164CysTyr: 0.164 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
7.341AspAla: 7.341 ± 0.06
0.43AspCys: 0.43 ± 0.017
3.692AspAsp: 3.692 ± 0.043
3.774AspGlu: 3.774 ± 0.052
1.502AspPhe: 1.502 ± 0.031
6.269AspGly: 6.269 ± 0.058
1.265AspHis: 1.265 ± 0.022
1.772AspIle: 1.772 ± 0.029
0.906AspLys: 0.906 ± 0.025
6.688AspLeu: 6.688 ± 0.065
0.708AspMet: 0.708 ± 0.02
1.04AspAsn: 1.04 ± 0.025
4.815AspPro: 4.815 ± 0.053
1.836AspGln: 1.836 ± 0.031
5.512AspArg: 5.512 ± 0.069
2.322AspSer: 2.322 ± 0.034
2.969AspThr: 2.969 ± 0.039
4.797AspVal: 4.797 ± 0.055
0.979AspTrp: 0.979 ± 0.022
1.162AspTyr: 1.162 ± 0.027
0.0AspXaa: 0.0 ± 0.0
Glu
6.411GluAla: 6.411 ± 0.071
0.346GluCys: 0.346 ± 0.014
1.951GluAsp: 1.951 ± 0.032
2.546GluGlu: 2.546 ± 0.041
1.484GluPhe: 1.484 ± 0.029
3.179GluGly: 3.179 ± 0.052
1.352GluHis: 1.352 ± 0.027
2.297GluIle: 2.297 ± 0.039
1.005GluLys: 1.005 ± 0.028
6.65GluLeu: 6.65 ± 0.067
0.841GluMet: 0.841 ± 0.021
0.898GluAsn: 0.898 ± 0.024
3.412GluPro: 3.412 ± 0.046
2.414GluGln: 2.414 ± 0.037
4.966GluArg: 4.966 ± 0.066
2.296GluSer: 2.296 ± 0.036
2.518GluThr: 2.518 ± 0.039
4.959GluVal: 4.959 ± 0.065
0.723GluTrp: 0.723 ± 0.023
1.087GluTyr: 1.087 ± 0.027
0.0GluXaa: 0.0 ± 0.0
Phe
3.724PheAla: 3.724 ± 0.047
0.241PheCys: 0.241 ± 0.012
1.965PheAsp: 1.965 ± 0.034
1.173PheGlu: 1.173 ± 0.027
0.844PhePhe: 0.844 ± 0.024
2.993PheGly: 2.993 ± 0.041
0.578PheHis: 0.578 ± 0.016
0.757PheIle: 0.757 ± 0.023
0.38PheLys: 0.38 ± 0.014
2.484PheLeu: 2.484 ± 0.046
0.318PheMet: 0.318 ± 0.013
0.582PheAsn: 0.582 ± 0.02
1.339PhePro: 1.339 ± 0.026
0.639PheGln: 0.639 ± 0.018
1.819PheArg: 1.819 ± 0.028
1.332PheSer: 1.332 ± 0.024
2.009PheThr: 2.009 ± 0.035
2.416PheVal: 2.416 ± 0.041
0.454PheTrp: 0.454 ± 0.018
0.593PheTyr: 0.593 ± 0.02
0.0PheXaa: 0.0 ± 0.0
Gly
10.414GlyAla: 10.414 ± 0.088
0.826GlyCys: 0.826 ± 0.025
5.119GlyAsp: 5.119 ± 0.055
4.704GlyGlu: 4.704 ± 0.061
2.72GlyPhe: 2.72 ± 0.039
8.666GlyGly: 8.666 ± 0.087
2.116GlyHis: 2.116 ± 0.039
3.256GlyIle: 3.256 ± 0.042
1.896GlyLys: 1.896 ± 0.038
9.533GlyLeu: 9.533 ± 0.085
1.82GlyMet: 1.82 ± 0.036
1.901GlyAsn: 1.901 ± 0.046
5.244GlyPro: 5.244 ± 0.063
2.959GlyGln: 2.959 ± 0.043
7.843GlyArg: 7.843 ± 0.075
4.797GlySer: 4.797 ± 0.048
5.972GlyThr: 5.972 ± 0.065
7.942GlyVal: 7.942 ± 0.065
1.918GlyTrp: 1.918 ± 0.032
2.439GlyTyr: 2.439 ± 0.041
0.0GlyXaa: 0.0 ± 0.0
His
2.459HisAla: 2.459 ± 0.042
0.177HisCys: 0.177 ± 0.011
1.361HisAsp: 1.361 ± 0.031
1.026HisGlu: 1.026 ± 0.026
0.557HisPhe: 0.557 ± 0.018
2.182HisGly: 2.182 ± 0.035
0.643HisHis: 0.643 ± 0.021
0.581HisIle: 0.581 ± 0.02
0.256HisLys: 0.256 ± 0.013
2.537HisLeu: 2.537 ± 0.041
0.267HisMet: 0.267 ± 0.012
0.353HisAsn: 0.353 ± 0.016
1.729HisPro: 1.729 ± 0.036
0.737HisGln: 0.737 ± 0.021
2.301HisArg: 2.301 ± 0.035
0.907HisSer: 0.907 ± 0.023
1.18HisThr: 1.18 ± 0.027
1.614HisVal: 1.614 ± 0.032
0.372HisTrp: 0.372 ± 0.014
0.439HisTyr: 0.439 ± 0.016
0.0HisXaa: 0.0 ± 0.0
Ile
4.93IleAla: 4.93 ± 0.054
0.325IleCys: 0.325 ± 0.013
2.474IleAsp: 2.474 ± 0.04
2.086IleGlu: 2.086 ± 0.038
0.929IlePhe: 0.929 ± 0.025
3.725IleGly: 3.725 ± 0.055
0.59IleHis: 0.59 ± 0.017
1.062IleIle: 1.062 ± 0.029
0.623IleLys: 0.623 ± 0.02
2.501IleLeu: 2.501 ± 0.044
0.453IleMet: 0.453 ± 0.013
0.799IleAsn: 0.799 ± 0.022
1.714IlePro: 1.714 ± 0.032
0.77IleGln: 0.77 ± 0.025
2.592IleArg: 2.592 ± 0.042
1.863IleSer: 1.863 ± 0.029
2.256IleThr: 2.256 ± 0.037
2.884IleVal: 2.884 ± 0.046
0.434IleTrp: 0.434 ± 0.016
0.639IleTyr: 0.639 ± 0.02
0.0IleXaa: 0.0 ± 0.0
Lys
2.03LysAla: 2.03 ± 0.043
0.099LysCys: 0.099 ± 0.008
0.776LysAsp: 0.776 ± 0.021
0.757LysGlu: 0.757 ± 0.024
0.402LysPhe: 0.402 ± 0.017
1.193LysGly: 1.193 ± 0.031
0.326LysHis: 0.326 ± 0.014
0.772LysIle: 0.772 ± 0.024
0.478LysLys: 0.478 ± 0.025
1.736LysLeu: 1.736 ± 0.032
0.287LysMet: 0.287 ± 0.013
0.348LysAsn: 0.348 ± 0.015
1.06LysPro: 1.06 ± 0.029
0.6LysGln: 0.6 ± 0.019
1.244LysArg: 1.244 ± 0.03
0.89LysSer: 0.89 ± 0.024
1.001LysThr: 1.001 ± 0.024
1.515LysVal: 1.515 ± 0.033
0.21LysTrp: 0.21 ± 0.012
0.339LysTyr: 0.339 ± 0.014
0.0LysXaa: 0.0 ± 0.0
Leu
16.678LeuAla: 16.678 ± 0.134
0.746LeuCys: 0.746 ± 0.021
7.015LeuAsp: 7.015 ± 0.064
3.795LeuGlu: 3.795 ± 0.052
2.56LeuPhe: 2.56 ± 0.04
8.99LeuGly: 8.99 ± 0.085
2.359LeuHis: 2.359 ± 0.042
3.514LeuIle: 3.514 ± 0.044
1.403LeuLys: 1.403 ± 0.03
12.143LeuLeu: 12.143 ± 0.115
1.373LeuMet: 1.373 ± 0.025
1.741LeuAsn: 1.741 ± 0.033
6.777LeuPro: 6.777 ± 0.076
1.89LeuGln: 1.89 ± 0.032
9.654LeuArg: 9.654 ± 0.088
5.004LeuSer: 5.004 ± 0.057
7.272LeuThr: 7.272 ± 0.063
10.053LeuVal: 10.053 ± 0.088
1.26LeuTrp: 1.26 ± 0.029
1.722LeuTyr: 1.722 ± 0.032
0.0LeuXaa: 0.0 ± 0.0
Met
2.044MetAla: 2.044 ± 0.038
0.116MetCys: 0.116 ± 0.008
0.676MetAsp: 0.676 ± 0.019
0.588MetGlu: 0.588 ± 0.017
0.446MetPhe: 0.446 ± 0.015
1.051MetGly: 1.051 ± 0.023
0.306MetHis: 0.306 ± 0.013
0.694MetIle: 0.694 ± 0.021
0.315MetLys: 0.315 ± 0.015
1.772MetLeu: 1.772 ± 0.031
0.268MetMet: 0.268 ± 0.012
0.357MetAsn: 0.357 ± 0.013
1.049MetPro: 1.049 ± 0.026
0.41MetGln: 0.41 ± 0.016
1.37MetArg: 1.37 ± 0.023
1.206MetSer: 1.206 ± 0.024
1.485MetThr: 1.485 ± 0.027
1.321MetVal: 1.321 ± 0.026
0.19MetTrp: 0.19 ± 0.011
0.276MetTyr: 0.276 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.237AsnAla: 2.237 ± 0.042
0.175AsnCys: 0.175 ± 0.009
1.009AsnAsp: 1.009 ± 0.027
0.824AsnGlu: 0.824 ± 0.023
0.554AsnPhe: 0.554 ± 0.018
1.98AsnGly: 1.98 ± 0.045
0.395AsnHis: 0.395 ± 0.017
0.645AsnIle: 0.645 ± 0.022
0.337AsnLys: 0.337 ± 0.014
1.934AsnLeu: 1.934 ± 0.038
0.293AsnMet: 0.293 ± 0.012
0.547AsnAsn: 0.547 ± 0.025
1.512AsnPro: 1.512 ± 0.036
0.609AsnGln: 0.609 ± 0.019
1.465AsnArg: 1.465 ± 0.028
0.977AsnSer: 0.977 ± 0.029
1.097AsnThr: 1.097 ± 0.028
1.464AsnVal: 1.464 ± 0.031
0.362AsnTrp: 0.362 ± 0.016
0.447AsnTyr: 0.447 ± 0.017
0.0AsnXaa: 0.0 ± 0.0
Pro
9.513ProAla: 9.513 ± 0.086
0.339ProCys: 0.339 ± 0.015
4.585ProAsp: 4.585 ± 0.052
3.86ProGlu: 3.86 ± 0.053
1.405ProPhe: 1.405 ± 0.03
6.34ProGly: 6.34 ± 0.068
1.268ProHis: 1.268 ± 0.029
1.713ProIle: 1.713 ± 0.03
0.947ProLys: 0.947 ± 0.028
5.307ProLeu: 5.307 ± 0.058
1.012ProMet: 1.012 ± 0.022
1.081ProAsn: 1.081 ± 0.028
4.201ProPro: 4.201 ± 0.075
1.795ProGln: 1.795 ± 0.032
3.951ProArg: 3.951 ± 0.048
3.008ProSer: 3.008 ± 0.047
4.097ProThr: 4.097 ± 0.063
5.79ProVal: 5.79 ± 0.071
0.946ProTrp: 0.946 ± 0.021
1.33ProTyr: 1.33 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
3.895GlnAla: 3.895 ± 0.05
0.15GlnCys: 0.15 ± 0.009
1.135GlnAsp: 1.135 ± 0.027
1.266GlnGlu: 1.266 ± 0.027
0.771GlnPhe: 0.771 ± 0.022
1.976GlnGly: 1.976 ± 0.036
0.697GlnHis: 0.697 ± 0.021
1.179GlnIle: 1.179 ± 0.026
0.397GlnLys: 0.397 ± 0.018
3.616GlnLeu: 3.616 ± 0.046
0.462GlnMet: 0.462 ± 0.014
0.478GlnAsn: 0.478 ± 0.018
2.006GlnPro: 2.006 ± 0.036
1.323GlnGln: 1.323 ± 0.029
2.974GlnArg: 2.974 ± 0.039
1.271GlnSer: 1.271 ± 0.028
1.471GlnThr: 1.471 ± 0.029
3.113GlnVal: 3.113 ± 0.042
0.562GlnTrp: 0.562 ± 0.02
0.572GlnTyr: 0.572 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
10.104ArgAla: 10.104 ± 0.095
0.66ArgCys: 0.66 ± 0.021
4.679ArgAsp: 4.679 ± 0.049
4.341ArgGlu: 4.341 ± 0.057
2.484ArgPhe: 2.484 ± 0.034
5.718ArgGly: 5.718 ± 0.064
2.294ArgHis: 2.294 ± 0.037
3.388ArgIle: 3.388 ± 0.043
1.317ArgLys: 1.317 ± 0.031
9.622ArgLeu: 9.622 ± 0.094
1.785ArgMet: 1.785 ± 0.032
1.519ArgAsn: 1.519 ± 0.029
5.396ArgPro: 5.396 ± 0.068
2.981ArgGln: 2.981 ± 0.042
8.82ArgArg: 8.82 ± 0.084
4.131ArgSer: 4.131 ± 0.051
4.982ArgThr: 4.982 ± 0.05
6.484ArgVal: 6.484 ± 0.062
1.715ArgTrp: 1.715 ± 0.029
2.044ArgTyr: 2.044 ± 0.033
0.0ArgXaa: 0.0 ± 0.0
Ser
6.421SerAla: 6.421 ± 0.065
0.38SerCys: 0.38 ± 0.016
2.426SerAsp: 2.426 ± 0.038
1.968SerGlu: 1.968 ± 0.034
1.442SerPhe: 1.442 ± 0.028
5.482SerGly: 5.482 ± 0.059
0.899SerHis: 0.899 ± 0.021
1.606SerIle: 1.606 ± 0.033
0.754SerLys: 0.754 ± 0.023
4.216SerLeu: 4.216 ± 0.048
0.98SerMet: 0.98 ± 0.021
0.931SerAsn: 0.931 ± 0.03
3.121SerPro: 3.121 ± 0.049
1.207SerGln: 1.207 ± 0.022
3.822SerArg: 3.822 ± 0.047
2.507SerSer: 2.507 ± 0.044
3.168SerThr: 3.168 ± 0.047
4.12SerVal: 4.12 ± 0.047
0.948SerTrp: 0.948 ± 0.023
1.299SerTyr: 1.299 ± 0.028
0.0SerXaa: 0.0 ± 0.0
Thr
8.767ThrAla: 8.767 ± 0.078
0.428ThrCys: 0.428 ± 0.017
3.795ThrAsp: 3.795 ± 0.052
3.188ThrGlu: 3.188 ± 0.047
1.69ThrPhe: 1.69 ± 0.031
6.701ThrGly: 6.701 ± 0.061
1.112ThrHis: 1.112 ± 0.02
2.137ThrIle: 2.137 ± 0.032
0.921ThrLys: 0.921 ± 0.024
5.934ThrLeu: 5.934 ± 0.054
1.023ThrMet: 1.023 ± 0.025
1.219ThrAsn: 1.219 ± 0.031
4.219ThrPro: 4.219 ± 0.055
1.374ThrGln: 1.374 ± 0.032
4.285ThrArg: 4.285 ± 0.053
3.056ThrSer: 3.056 ± 0.044
3.866ThrThr: 3.866 ± 0.059
6.437ThrVal: 6.437 ± 0.062
0.931ThrTrp: 0.931 ± 0.027
1.277ThrTyr: 1.277 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
12.686ValAla: 12.686 ± 0.099
0.719ValCys: 0.719 ± 0.017
5.758ValAsp: 5.758 ± 0.054
4.752ValGlu: 4.752 ± 0.053
2.3ValPhe: 2.3 ± 0.039
7.494ValGly: 7.494 ± 0.077
1.787ValHis: 1.787 ± 0.034
3.108ValIle: 3.108 ± 0.044
1.347ValLys: 1.347 ± 0.03
9.931ValLeu: 9.931 ± 0.088
1.161ValMet: 1.161 ± 0.025
1.854ValAsn: 1.854 ± 0.033
5.412ValPro: 5.412 ± 0.059
2.158ValGln: 2.158 ± 0.036
7.288ValArg: 7.288 ± 0.065
4.351ValSer: 4.351 ± 0.054
6.232ValThr: 6.232 ± 0.061
8.671ValVal: 8.671 ± 0.086
1.101ValTrp: 1.101 ± 0.026
1.453ValTyr: 1.453 ± 0.027
0.0ValXaa: 0.0 ± 0.0
Trp
1.679TrpAla: 1.679 ± 0.036
0.162TrpCys: 0.162 ± 0.01
0.724TrpAsp: 0.724 ± 0.023
0.643TrpGlu: 0.643 ± 0.018
0.506TrpPhe: 0.506 ± 0.017
0.967TrpGly: 0.967 ± 0.024
0.421TrpHis: 0.421 ± 0.013
0.539TrpIle: 0.539 ± 0.018
0.258TrpLys: 0.258 ± 0.01
2.043TrpLeu: 2.043 ± 0.036
0.259TrpMet: 0.259 ± 0.011
0.418TrpAsn: 0.418 ± 0.019
0.957TrpPro: 0.957 ± 0.023
0.738TrpGln: 0.738 ± 0.023
1.662TrpArg: 1.662 ± 0.027
1.023TrpSer: 1.023 ± 0.025
1.031TrpThr: 1.031 ± 0.027
1.092TrpVal: 1.092 ± 0.027
0.414TrpTrp: 0.414 ± 0.014
0.382TrpTyr: 0.382 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.918TyrAla: 2.918 ± 0.042
0.171TyrCys: 0.171 ± 0.009
1.449TyrAsp: 1.449 ± 0.032
1.075TyrGlu: 1.075 ± 0.026
0.633TyrPhe: 0.633 ± 0.017
2.099TyrGly: 2.099 ± 0.041
0.493TyrHis: 0.493 ± 0.017
0.433TyrIle: 0.433 ± 0.016
0.279TyrLys: 0.279 ± 0.013
2.365TyrLeu: 2.365 ± 0.038
0.172TyrMet: 0.172 ± 0.01
0.401TyrAsn: 0.401 ± 0.018
1.237TyrPro: 1.237 ± 0.029
0.785TyrGln: 0.785 ± 0.022
1.951TyrArg: 1.951 ± 0.035
0.863TyrSer: 0.863 ± 0.022
1.117TyrThr: 1.117 ± 0.025
1.706TyrVal: 1.706 ± 0.032
0.35TyrTrp: 0.35 ± 0.013
0.465TyrTyr: 0.465 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5896 proteins (1875034 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski