Brugia pahangi (Filarial nematode worm)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Spirurina; Spiruromorpha; Filarioidea; Onchocercidae; Brugia

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14545 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N4TC44|A0A0N4TC44_BRUPA Uncharacterized protein OS=Brugia pahangi OX=6280 GN=BPAG_LOCUS5744 PE=4 SV=1
MM1 pKa = 7.17RR2 pKa = 11.84QLYY5 pKa = 7.46VWCYY9 pKa = 10.42IDD11 pKa = 5.22IGLQGTEE18 pKa = 4.06EE19 pKa = 4.55SVSSAHH25 pKa = 6.16NGNGIEE31 pKa = 4.16EE32 pKa = 3.94QAEE35 pKa = 4.11YY36 pKa = 10.92DD37 pKa = 3.67EE38 pKa = 5.25KK39 pKa = 10.94RR40 pKa = 11.84AEE42 pKa = 4.17NGCGDD47 pKa = 5.58DD48 pKa = 5.29IDD50 pKa = 6.57DD51 pKa = 6.24DD52 pKa = 6.18GDD54 pKa = 5.06DD55 pKa = 4.58DD56 pKa = 5.85DD57 pKa = 5.13KK58 pKa = 12.05AHH60 pKa = 6.41LAIRR64 pKa = 11.84FSPQDD69 pKa = 3.14KK70 pKa = 10.19TILQNIYY77 pKa = 9.71QQMCEE82 pKa = 4.16CQEE85 pKa = 4.15LNPDD89 pKa = 3.79EE90 pKa = 6.04GDD92 pKa = 3.92DD93 pKa = 4.08FSDD96 pKa = 5.15DD97 pKa = 3.48FTMDD101 pKa = 3.53PDD103 pKa = 4.62GDD105 pKa = 3.73FSEE108 pKa = 4.53NKK110 pKa = 9.75SDD112 pKa = 4.25EE113 pKa = 4.31EE114 pKa = 3.81EE115 pKa = 4.67DD116 pKa = 3.93YY117 pKa = 11.38EE118 pKa = 5.13GNDD121 pKa = 4.53GEE123 pKa = 5.06GDD125 pKa = 3.6QNTLYY130 pKa = 10.83FQDD133 pKa = 3.07ISTNMRR139 pKa = 11.84RR140 pKa = 11.84HH141 pKa = 5.08EE142 pKa = 4.33TNGAYY147 pKa = 9.07MLRR150 pKa = 11.84IADD153 pKa = 4.03HH154 pKa = 6.82GDD156 pKa = 3.3AFGSEE161 pKa = 4.03QMDD164 pKa = 3.58EE165 pKa = 4.29GG166 pKa = 4.09

Molecular weight: 18.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N4TEV8|A0A0N4TEV8_BRUPA Uncharacterized protein OS=Brugia pahangi OX=6280 GN=BPAG_LOCUS4723 PE=4 SV=1
MM1 pKa = 6.37QAKK4 pKa = 8.35RR5 pKa = 11.84TGQGMQAKK13 pKa = 8.37RR14 pKa = 11.84TGQGMQAKK22 pKa = 8.37RR23 pKa = 11.84TGQGMQAKK31 pKa = 8.37RR32 pKa = 11.84TGQGMQAKK40 pKa = 8.37RR41 pKa = 11.84TGQGMQAKK49 pKa = 8.37RR50 pKa = 11.84TGQGMQAKK58 pKa = 8.37RR59 pKa = 11.84TGQGMQAKK67 pKa = 8.37RR68 pKa = 11.84TGQGMQQ74 pKa = 3.04

Molecular weight: 7.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14545

0

14545

5004100

24

7308

344.0

39.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.002 ± 0.018

2.269 ± 0.017

5.339 ± 0.02

6.447 ± 0.029

4.374 ± 0.017

4.976 ± 0.026

2.435 ± 0.011

6.754 ± 0.025

6.177 ± 0.024

9.367 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.551 ± 0.01

5.209 ± 0.019

4.166 ± 0.022

4.191 ± 0.02

5.639 ± 0.021

8.222 ± 0.027

5.616 ± 0.02

5.889 ± 0.017

1.102 ± 0.007

3.271 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski