Streptococcus satellite phage Javan360
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZQZ7|A0A4D5ZQZ7_9VIRU Glycerate kinase OS=Streptococcus satellite phage Javan360 OX=2558660 GN=JavanS360_0015 PE=4 SV=1
MM1 pKa = 7.6 EE2 pKa = 5.96 KK3 pKa = 10.91 NMTLDD8 pKa = 4.31 LDD10 pKa = 3.98 NMTQAEE16 pKa = 4.34 FDD18 pKa = 3.6 NRR20 pKa = 11.84 ITEE23 pKa = 4.12 IKK25 pKa = 10.3 DD26 pKa = 3.2 RR27 pKa = 11.84 NPNLFQFIIDD37 pKa = 3.88 FLDD40 pKa = 4.06 DD41 pKa = 3.54 KK42 pKa = 10.4 VTPEE46 pKa = 3.89 EE47 pKa = 4.34 VYY49 pKa = 11.05 DD50 pKa = 3.84 FLKK53 pKa = 10.18 MEE55 pKa = 4.85 RR56 pKa = 11.84 SYY58 pKa = 11.18 QVNYY62 pKa = 9.3 IKK64 pKa = 10.67 NYY66 pKa = 6.38 QARR69 pKa = 11.84 AA70 pKa = 3.37
Molecular weight: 8.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.582
IPC2_protein 4.533
IPC_protein 4.38
Toseland 4.202
ProMoST 4.52
Dawson 4.342
Bjellqvist 4.507
Wikipedia 4.253
Rodwell 4.215
Grimsley 4.113
Solomon 4.342
Lehninger 4.291
Nozaki 4.469
DTASelect 4.66
Thurlkill 4.228
EMBOSS 4.266
Sillero 4.495
Patrickios 4.024
IPC_peptide 4.342
IPC2_peptide 4.482
IPC2.peptide.svr19 4.453
Protein with the highest isoelectric point:
>tr|A0A4D5ZKJ4|A0A4D5ZKJ4_9VIRU Helicase loader OS=Streptococcus satellite phage Javan360 OX=2558660 GN=JavanS360_0012 PE=4 SV=1
MM1 pKa = 7.38 AVAEE5 pKa = 4.17 TSLVNKK11 pKa = 9.01 NHH13 pKa = 6.31 QIPRR17 pKa = 11.84 IINQKK22 pKa = 9.38 IAHH25 pKa = 5.97 QLSISTSKK33 pKa = 10.88 VIRR36 pKa = 11.84 KK37 pKa = 9.18 FNDD40 pKa = 2.87 FHH42 pKa = 6.95 FKK44 pKa = 10.89 LDD46 pKa = 4.1 FSHH49 pKa = 7.25 LPEE52 pKa = 5.34 IMSWDD57 pKa = 3.32 VYY59 pKa = 11.57 AFTKK63 pKa = 10.87 GKK65 pKa = 9.35 MSFIAQDD72 pKa = 3.67 FDD74 pKa = 3.74 NLSIIAVLEE83 pKa = 4.05 GRR85 pKa = 11.84 TQAVIRR91 pKa = 11.84 NHH93 pKa = 5.2 YY94 pKa = 9.53 LRR96 pKa = 11.84 YY97 pKa = 10.06 DD98 pKa = 3.48 RR99 pKa = 11.84 AVRR102 pKa = 11.84 CQVKK106 pKa = 10.1 IITMDD111 pKa = 3.33 MFSPYY116 pKa = 10.32 YY117 pKa = 10.52 DD118 pKa = 3.23 LAKK121 pKa = 10.15 QLRR124 pKa = 11.84 VQISRR129 pKa = 11.84 LRR131 pKa = 11.84 LKK133 pKa = 10.2 QSPRR137 pKa = 11.84 LFHH140 pKa = 6.93 SRR142 pKa = 11.84 VLKK145 pKa = 10.79 SFLIAFTLYY154 pKa = 10.86 NII156 pKa = 4.51
Molecular weight: 18.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.244
IPC2_protein 9.604
IPC_protein 9.853
Toseland 10.335
ProMoST 9.97
Dawson 10.482
Bjellqvist 10.145
Wikipedia 10.643
Rodwell 10.921
Grimsley 10.54
Solomon 10.526
Lehninger 10.496
Nozaki 10.306
DTASelect 10.145
Thurlkill 10.35
EMBOSS 10.716
Sillero 10.394
Patrickios 10.613
IPC_peptide 10.526
IPC2_peptide 8.829
IPC2.peptide.svr19 8.67
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
21
0
21
3871
53
415
184.3
21.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.373 ± 0.538
0.542 ± 0.116
5.89 ± 0.413
8.37 ± 0.532
4.702 ± 0.414
4.133 ± 0.368
1.421 ± 0.27
6.897 ± 0.479
10.256 ± 0.555
10.643 ± 0.518
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.17 ± 0.264
5.296 ± 0.296
1.963 ± 0.217
4.702 ± 0.61
4.779 ± 0.438
6.432 ± 0.392
6.097 ± 0.492
5.58 ± 0.479
0.723 ± 0.103
4.03 ± 0.37
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here