Veillonella montpellierensis DNF00314
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1513 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A096AKJ3|A0A096AKJ3_9FIRM Uncharacterized protein OS=Veillonella montpellierensis DNF00314 OX=1401067 GN=HMPREF0872_04365 PE=4 SV=1
MM1 pKa = 7.92 CIQLDD6 pKa = 3.84 SFAIVQDD13 pKa = 3.39 GEE15 pKa = 4.32 NYY17 pKa = 9.21 YY18 pKa = 10.62 KK19 pKa = 10.42 EE20 pKa = 4.15 YY21 pKa = 10.58 SNEE24 pKa = 3.82 YY25 pKa = 8.28 TGEE28 pKa = 4.18 GEE30 pKa = 4.27 ILCEE34 pKa = 4.53 IPAIDD39 pKa = 5.2 DD40 pKa = 3.7 GVEE43 pKa = 3.85 ALDD46 pKa = 3.89 NLFKK50 pKa = 10.89 RR51 pKa = 11.84 LPSFITIEE59 pKa = 4.02 EE60 pKa = 4.22 MFDD63 pKa = 3.08 IAIKK67 pKa = 10.65 CKK69 pKa = 10.11 LATINDD75 pKa = 3.33 IKK77 pKa = 10.94 EE78 pKa = 4.04 WIVNGNCEE86 pKa = 3.74
Molecular weight: 9.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.788
IPC2_protein 3.986
IPC_protein 3.884
Toseland 3.706
ProMoST 3.986
Dawson 3.846
Bjellqvist 4.075
Wikipedia 3.745
Rodwell 3.719
Grimsley 3.63
Solomon 3.834
Lehninger 3.783
Nozaki 3.973
DTASelect 4.113
Thurlkill 3.745
EMBOSS 3.757
Sillero 3.999
Patrickios 0.846
IPC_peptide 3.834
IPC2_peptide 3.986
IPC2.peptide.svr19 3.941
Protein with the highest isoelectric point:
>tr|A0A096BVW9|A0A096BVW9_9FIRM Nitrate reductase OS=Veillonella montpellierensis DNF00314 OX=1401067 GN=HMPREF0872_06985 PE=4 SV=1
MM1 pKa = 7.43 NGLRR5 pKa = 11.84 KK6 pKa = 9.25 EE7 pKa = 3.89 RR8 pKa = 11.84 RR9 pKa = 11.84 ITKK12 pKa = 8.94 NSEE15 pKa = 3.27 YY16 pKa = 10.61 RR17 pKa = 11.84 LVYY20 pKa = 10.17 KK21 pKa = 10.44 HH22 pKa = 6.24 GNYY25 pKa = 9.68 EE26 pKa = 3.91 VGRR29 pKa = 11.84 LCVIYY34 pKa = 10.35 RR35 pKa = 11.84 MPVAKK40 pKa = 9.94 QATRR44 pKa = 11.84 IGFVTGKK51 pKa = 10.43 KK52 pKa = 9.68 VGCAVEE58 pKa = 4.14 RR59 pKa = 11.84 NRR61 pKa = 11.84 ARR63 pKa = 11.84 RR64 pKa = 11.84 LLKK67 pKa = 9.92 EE68 pKa = 4.02 VYY70 pKa = 9.6 RR71 pKa = 11.84 LHH73 pKa = 6.1 QHH75 pKa = 7.3 DD76 pKa = 3.78 IRR78 pKa = 11.84 EE79 pKa = 4.72 GYY81 pKa = 9.59 HH82 pKa = 6.34 IVMIGRR88 pKa = 11.84 AKK90 pKa = 10.88 LSTATYY96 pKa = 9.46 AQAEE100 pKa = 4.31 KK101 pKa = 10.6 EE102 pKa = 3.93 ILYY105 pKa = 10.79 LLGRR109 pKa = 11.84 SKK111 pKa = 11.14 LMKK114 pKa = 10.36 SS115 pKa = 3.3
Molecular weight: 13.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.317
IPC2_protein 9.897
IPC_protein 10.54
Toseland 10.57
ProMoST 10.262
Dawson 10.716
Bjellqvist 10.409
Wikipedia 10.906
Rodwell 11.052
Grimsley 10.774
Solomon 10.774
Lehninger 10.745
Nozaki 10.555
DTASelect 10.409
Thurlkill 10.584
EMBOSS 10.965
Sillero 10.628
Patrickios 10.745
IPC_peptide 10.774
IPC2_peptide 9.326
IPC2.peptide.svr19 8.47
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1513
0
1513
482565
37
3115
318.9
35.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.788 ± 0.067
1.168 ± 0.022
5.644 ± 0.057
6.466 ± 0.072
3.626 ± 0.045
7.177 ± 0.076
2.402 ± 0.038
8.042 ± 0.075
6.244 ± 0.068
9.019 ± 0.074
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.004 ± 0.034
4.258 ± 0.066
3.497 ± 0.037
3.47 ± 0.038
4.441 ± 0.054
5.727 ± 0.047
6.114 ± 0.059
7.327 ± 0.05
0.892 ± 0.021
3.695 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here