Bowdeniella nasicola

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Bowdeniella

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2401 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q5PUR9|A0A1Q5PUR9_9ACTO Mutator family transposase (Fragment) OS=Bowdeniella nasicola OX=208480 GN=BSZ39_12780 PE=3 SV=1
MM1 pKa = 7.75IDD3 pKa = 3.43GFFVGWPSPPNAQTLIDD20 pKa = 4.17VMDD23 pKa = 4.43GSYY26 pKa = 10.73RR27 pKa = 11.84RR28 pKa = 11.84IWAVADD34 pKa = 4.02GKK36 pKa = 10.99VVGYY40 pKa = 10.29INAISDD46 pKa = 3.68GVLNAFIPWLEE57 pKa = 3.81VHH59 pKa = 7.24PDD61 pKa = 3.49YY62 pKa = 11.2QEE64 pKa = 3.7QGIGRR69 pKa = 11.84EE70 pKa = 3.91LVEE73 pKa = 4.38RR74 pKa = 11.84MVAEE78 pKa = 4.43LEE80 pKa = 4.06SMYY83 pKa = 10.77AIDD86 pKa = 5.36LMCDD90 pKa = 3.24EE91 pKa = 4.99EE92 pKa = 5.15LIPYY96 pKa = 8.45YY97 pKa = 10.54EE98 pKa = 4.37KK99 pKa = 11.0LGFMGSSEE107 pKa = 4.55LSVGAPIGQGCWGG120 pKa = 3.6

Molecular weight: 13.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q5Q0K1|A0A1Q5Q0K1_9ACTO Haloacid dehalogenase OS=Bowdeniella nasicola OX=208480 GN=BSZ39_10750 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILSSRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.86GRR40 pKa = 11.84AKK42 pKa = 10.68LSAA45 pKa = 3.92

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2401

0

2401

758322

26

1985

315.8

34.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.756 ± 0.058

0.743 ± 0.015

6.189 ± 0.047

5.959 ± 0.053

3.115 ± 0.033

8.333 ± 0.047

2.183 ± 0.027

5.161 ± 0.035

2.849 ± 0.038

9.954 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.094 ± 0.022

2.233 ± 0.025

5.295 ± 0.036

2.872 ± 0.026

6.821 ± 0.056

5.672 ± 0.03

6.118 ± 0.035

8.112 ± 0.041

1.38 ± 0.02

2.161 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski