Eoetvoesia caeni
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4508 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A366H7Z9|A0A366H7Z9_9BURK Thiosulfate dehydrogenase OS=Eoetvoesia caeni OX=645616 GN=DFR37_10787 PE=4 SV=1
MM1 pKa = 7.67 SDD3 pKa = 2.86 IPEE6 pKa = 4.4 NFDD9 pKa = 3.11 TDD11 pKa = 3.28 IATLVAALSCGDD23 pKa = 3.86 LDD25 pKa = 3.48 EE26 pKa = 6.7 HH27 pKa = 6.64 EE28 pKa = 4.37 IARR31 pKa = 11.84 IVGICRR37 pKa = 11.84 QALEE41 pKa = 4.22 NPAAVLAEE49 pKa = 4.13 NYY51 pKa = 8.05 GTEE54 pKa = 4.11 AAEE57 pKa = 4.18 VAQYY61 pKa = 11.1 DD62 pKa = 4.01 PQGVIAFIVFIEE74 pKa = 4.29 LEE76 pKa = 4.35 DD77 pKa = 3.84 YY78 pKa = 10.69 FAVADD83 pKa = 4.2 TVDD86 pKa = 3.83 EE87 pKa = 4.12 LHH89 pKa = 6.37 EE90 pKa = 4.08 QIIAAFEE97 pKa = 4.1 APQLPDD103 pKa = 3.43 YY104 pKa = 10.69 PYY106 pKa = 11.05 DD107 pKa = 4.37 DD108 pKa = 4.65 NDD110 pKa = 3.64 FEE112 pKa = 4.68 TVSDD116 pKa = 3.87 YY117 pKa = 10.56 FQWLDD122 pKa = 3.37 AQLLEE127 pKa = 3.93 HH128 pKa = 6.61 HH129 pKa = 6.4 AAYY132 pKa = 10.54 RR133 pKa = 11.84 LIHH136 pKa = 6.43 FGQSYY141 pKa = 9.67 TNDD144 pKa = 3.17 FQTVLVHH151 pKa = 7.61 RR152 pKa = 11.84 EE153 pKa = 4.13 GTDD156 pKa = 3.14 SLLALCRR163 pKa = 11.84 KK164 pKa = 9.66 LKK166 pKa = 11.03 LEE168 pKa = 4.62 AEE170 pKa = 4.41 YY171 pKa = 11.37 CEE173 pKa = 4.23
Molecular weight: 19.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.831
IPC2_protein 3.923
IPC_protein 3.897
Toseland 3.706
ProMoST 4.012
Dawson 3.872
Bjellqvist 4.062
Wikipedia 3.783
Rodwell 3.732
Grimsley 3.617
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.177
Thurlkill 3.745
EMBOSS 3.795
Sillero 4.012
Patrickios 0.451
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.928
Protein with the highest isoelectric point:
>tr|A0A366HHH1|A0A366HHH1_9BURK Xanthine dehydrogenase YagT iron-sulfur-binding subunit OS=Eoetvoesia caeni OX=645616 GN=DFR37_102393 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.43 RR14 pKa = 11.84 THH16 pKa = 5.88 GFLVRR21 pKa = 11.84 MKK23 pKa = 9.7 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVIKK32 pKa = 10.36 ARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.79 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4508
0
4508
1422334
27
3222
315.5
34.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.625 ± 0.043
0.943 ± 0.012
5.29 ± 0.033
5.335 ± 0.035
3.568 ± 0.023
8.012 ± 0.038
2.29 ± 0.017
5.116 ± 0.025
3.584 ± 0.029
10.607 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.533 ± 0.019
3.0 ± 0.02
5.048 ± 0.022
4.227 ± 0.023
6.455 ± 0.04
5.842 ± 0.029
5.209 ± 0.038
7.328 ± 0.033
1.441 ± 0.014
2.546 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here