Streptococcus phage Javan499

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 39 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AED4|A0A4D6AED4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan499 OX=2548213 GN=Javan499_0037 PE=4 SV=1
MM1 pKa = 6.71WTDD4 pKa = 3.72GRR6 pKa = 11.84IDD8 pKa = 3.66YY9 pKa = 10.43QGQKK13 pKa = 9.41VDD15 pKa = 4.19YY16 pKa = 8.62IAKK19 pKa = 9.96VSPQPSEE26 pKa = 3.82VGIDD30 pKa = 3.45LGCIFKK36 pKa = 10.81LDD38 pKa = 3.41IEE40 pKa = 4.58VAEE43 pKa = 4.24EE44 pKa = 4.3TIVSYY49 pKa = 11.21DD50 pKa = 3.89RR51 pKa = 11.84GWEE54 pKa = 4.01LYY56 pKa = 9.94PEE58 pKa = 4.3TEE60 pKa = 3.88EE61 pKa = 5.06RR62 pKa = 11.84EE63 pKa = 4.47AILEE67 pKa = 4.11AVLMVLTVV75 pKa = 3.15

Molecular weight: 8.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AGE6|A0A4D6AGE6_9CAUD Uncharacterized protein OS=Streptococcus phage Javan499 OX=2548213 GN=Javan499_0010 PE=4 SV=1
MM1 pKa = 7.72MYY3 pKa = 10.16TILTCTIMGLWVLIGLYY20 pKa = 9.77FGYY23 pKa = 8.1MTIRR27 pKa = 11.84DD28 pKa = 4.31DD29 pKa = 3.5IRR31 pKa = 11.84NEE33 pKa = 3.88VEE35 pKa = 5.19RR36 pKa = 11.84KK37 pKa = 9.58AKK39 pKa = 9.31QNKK42 pKa = 8.32EE43 pKa = 4.09KK44 pKa = 10.83LSQTPLSRR52 pKa = 11.84KK53 pKa = 9.46NKK55 pKa = 9.68

Molecular weight: 6.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

39

0

39

11178

38

1039

286.6

32.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.54 ± 0.528

0.814 ± 0.121

5.672 ± 0.304

7.363 ± 0.278

3.874 ± 0.183

7.032 ± 0.341

1.798 ± 0.169

6.227 ± 0.342

7.336 ± 0.314

8.83 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.433 ± 0.167

4.733 ± 0.206

3.185 ± 0.218

4.294 ± 0.225

4.536 ± 0.304

7.032 ± 0.411

6.352 ± 0.387

7.005 ± 0.25

1.405 ± 0.105

3.543 ± 0.279
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski