Nonomuraea zeae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Streptosporangiaceae; Nonomuraea

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12011 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5S4HJQ2|A0A5S4HJQ2_9ACTN VOC family protein OS=Nonomuraea zeae OX=1642303 GN=ETD85_01335 PE=4 SV=1
MM1 pKa = 7.41KK2 pKa = 10.29NDD4 pKa = 3.53WLIGDD9 pKa = 5.21DD10 pKa = 4.23GTDD13 pKa = 3.49ASDD16 pKa = 4.02PDD18 pKa = 3.86ASAICHH24 pKa = 5.51VADD27 pKa = 5.05VVDD30 pKa = 5.3LDD32 pKa = 3.94PSVAEE37 pKa = 4.35TASLPCGYY45 pKa = 10.52AARR48 pKa = 11.84RR49 pKa = 11.84TSKK52 pKa = 10.61EE53 pKa = 4.03EE54 pKa = 3.42PWVIEE59 pKa = 3.4EE60 pKa = 4.35WYY62 pKa = 10.81YY63 pKa = 10.89EE64 pKa = 4.2EE65 pKa = 4.92EE66 pKa = 4.33VV67 pKa = 3.41

Molecular weight: 7.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5S4GXH4|A0A5S4GXH4_9ACTN Maleylpyruvate isomerase family mycothiol-dependent enzyme OS=Nonomuraea zeae OX=1642303 GN=ETD85_26705 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84HH37 pKa = 5.69KK38 pKa = 10.79GRR40 pKa = 11.84AKK42 pKa = 10.69LSAA45 pKa = 3.92

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12011

0

12011

3909505

27

4931

325.5

34.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.414 ± 0.033

0.773 ± 0.006

5.702 ± 0.018

5.538 ± 0.021

2.849 ± 0.013

9.466 ± 0.025

2.214 ± 0.013

3.614 ± 0.017

2.032 ± 0.017

10.714 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.857 ± 0.009

1.787 ± 0.014

5.98 ± 0.017

2.782 ± 0.013

8.035 ± 0.026

5.103 ± 0.015

5.815 ± 0.022

8.543 ± 0.024

1.593 ± 0.009

2.189 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski