Amino acid dipepetide frequency for Escherichia phage vB_Eco_mar001J1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.737AlaAla: 6.737 ± 0.889
0.793AlaCys: 0.793 ± 0.234
3.435AlaAsp: 3.435 ± 0.574
4.822AlaGlu: 4.822 ± 0.799
3.303AlaPhe: 3.303 ± 0.604
4.888AlaGly: 4.888 ± 0.515
1.189AlaHis: 1.189 ± 0.274
5.746AlaIle: 5.746 ± 0.753
6.605AlaLys: 6.605 ± 0.76
6.011AlaLeu: 6.011 ± 0.706
2.708AlaMet: 2.708 ± 0.403
4.029AlaAsn: 4.029 ± 0.629
2.048AlaPro: 2.048 ± 0.336
3.236AlaGln: 3.236 ± 0.386
4.227AlaArg: 4.227 ± 0.529
5.086AlaSer: 5.086 ± 0.504
3.567AlaThr: 3.567 ± 0.475
5.68AlaVal: 5.68 ± 0.665
0.991AlaTrp: 0.991 ± 0.205
3.038AlaTyr: 3.038 ± 0.482
0.0AlaXaa: 0.0 ± 0.0
Cys
1.123CysAla: 1.123 ± 0.288
0.198CysCys: 0.198 ± 0.113
1.387CysAsp: 1.387 ± 0.316
0.991CysGlu: 0.991 ± 0.277
0.396CysPhe: 0.396 ± 0.155
1.321CysGly: 1.321 ± 0.367
0.198CysHis: 0.198 ± 0.151
0.462CysIle: 0.462 ± 0.182
0.859CysLys: 0.859 ± 0.293
0.793CysLeu: 0.793 ± 0.212
0.462CysMet: 0.462 ± 0.175
0.462CysAsn: 0.462 ± 0.197
0.462CysPro: 0.462 ± 0.152
0.198CysGln: 0.198 ± 0.157
1.189CysArg: 1.189 ± 0.358
0.594CysSer: 0.594 ± 0.192
0.661CysThr: 0.661 ± 0.167
1.123CysVal: 1.123 ± 0.258
0.396CysTrp: 0.396 ± 0.165
0.528CysTyr: 0.528 ± 0.165
0.0CysXaa: 0.0 ± 0.0
Asp
4.822AspAla: 4.822 ± 0.671
0.727AspCys: 0.727 ± 0.241
3.963AspAsp: 3.963 ± 0.556
4.029AspGlu: 4.029 ± 0.484
2.708AspPhe: 2.708 ± 0.403
7.332AspGly: 7.332 ± 0.861
0.925AspHis: 0.925 ± 0.231
3.435AspIle: 3.435 ± 0.459
4.161AspLys: 4.161 ± 0.514
4.095AspLeu: 4.095 ± 0.536
2.246AspMet: 2.246 ± 0.315
2.51AspAsn: 2.51 ± 0.465
2.378AspPro: 2.378 ± 0.35
1.651AspGln: 1.651 ± 0.284
2.84AspArg: 2.84 ± 0.47
3.831AspSer: 3.831 ± 0.453
3.369AspThr: 3.369 ± 0.513
4.756AspVal: 4.756 ± 0.561
1.189AspTrp: 1.189 ± 0.238
2.18AspTyr: 2.18 ± 0.36
0.0AspXaa: 0.0 ± 0.0
Glu
5.548GluAla: 5.548 ± 0.612
1.123GluCys: 1.123 ± 0.254
3.369GluAsp: 3.369 ± 0.458
4.557GluGlu: 4.557 ± 0.67
2.84GluPhe: 2.84 ± 0.512
3.236GluGly: 3.236 ± 0.544
0.991GluHis: 0.991 ± 0.265
5.416GluIle: 5.416 ± 0.654
4.491GluLys: 4.491 ± 0.573
4.425GluLeu: 4.425 ± 0.849
2.576GluMet: 2.576 ± 0.361
3.897GluAsn: 3.897 ± 0.431
1.717GluPro: 1.717 ± 0.331
3.038GluGln: 3.038 ± 0.527
3.369GluArg: 3.369 ± 0.455
4.161GluSer: 4.161 ± 0.395
3.435GluThr: 3.435 ± 0.449
4.624GluVal: 4.624 ± 0.628
0.793GluTrp: 0.793 ± 0.215
3.104GluTyr: 3.104 ± 0.467
0.0GluXaa: 0.0 ± 0.0
Phe
2.642PheAla: 2.642 ± 0.413
0.594PheCys: 0.594 ± 0.171
3.17PheAsp: 3.17 ± 0.578
2.114PheGlu: 2.114 ± 0.301
1.387PhePhe: 1.387 ± 0.365
3.633PheGly: 3.633 ± 0.463
0.727PheHis: 0.727 ± 0.212
2.312PheIle: 2.312 ± 0.364
3.17PheLys: 3.17 ± 0.417
2.312PheLeu: 2.312 ± 0.459
1.057PheMet: 1.057 ± 0.32
2.18PheAsn: 2.18 ± 0.356
1.255PhePro: 1.255 ± 0.287
1.585PheGln: 1.585 ± 0.274
2.048PheArg: 2.048 ± 0.281
2.18PheSer: 2.18 ± 0.364
2.642PheThr: 2.642 ± 0.503
2.708PheVal: 2.708 ± 0.37
0.991PheTrp: 0.991 ± 0.253
1.453PheTyr: 1.453 ± 0.379
0.0PheXaa: 0.0 ± 0.0
Gly
4.822GlyAla: 4.822 ± 0.694
1.453GlyCys: 1.453 ± 0.348
5.086GlyAsp: 5.086 ± 0.705
4.888GlyGlu: 4.888 ± 0.561
3.567GlyPhe: 3.567 ± 0.454
6.935GlyGly: 6.935 ± 1.04
1.387GlyHis: 1.387 ± 0.383
4.491GlyIle: 4.491 ± 0.631
6.473GlyLys: 6.473 ± 0.686
5.482GlyLeu: 5.482 ± 0.606
2.642GlyMet: 2.642 ± 0.437
3.303GlyAsn: 3.303 ± 0.365
0.396GlyPro: 0.396 ± 0.208
2.18GlyGln: 2.18 ± 0.29
3.567GlyArg: 3.567 ± 0.516
5.284GlySer: 5.284 ± 0.624
2.114GlyThr: 2.114 ± 0.404
5.878GlyVal: 5.878 ± 0.515
1.585GlyTrp: 1.585 ± 0.334
4.029GlyTyr: 4.029 ± 0.55
0.0GlyXaa: 0.0 ± 0.0
His
0.925HisAla: 0.925 ± 0.234
0.33HisCys: 0.33 ± 0.131
1.453HisAsp: 1.453 ± 0.271
1.321HisGlu: 1.321 ± 0.294
0.594HisPhe: 0.594 ± 0.165
1.783HisGly: 1.783 ± 0.436
0.528HisHis: 0.528 ± 0.256
0.991HisIle: 0.991 ± 0.291
1.123HisLys: 1.123 ± 0.313
0.991HisLeu: 0.991 ± 0.262
0.396HisMet: 0.396 ± 0.195
0.991HisAsn: 0.991 ± 0.249
0.727HisPro: 0.727 ± 0.208
0.594HisGln: 0.594 ± 0.206
1.189HisArg: 1.189 ± 0.311
0.991HisSer: 0.991 ± 0.293
0.727HisThr: 0.727 ± 0.222
1.783HisVal: 1.783 ± 0.34
0.264HisTrp: 0.264 ± 0.135
1.255HisTyr: 1.255 ± 0.335
0.0HisXaa: 0.0 ± 0.0
Ile
5.945IleAla: 5.945 ± 0.513
1.189IleCys: 1.189 ± 0.367
5.482IleAsp: 5.482 ± 0.61
4.095IleGlu: 4.095 ± 0.405
1.651IlePhe: 1.651 ± 0.234
3.765IleGly: 3.765 ± 0.419
1.453IleHis: 1.453 ± 0.336
3.567IleIle: 3.567 ± 0.433
5.086IleLys: 5.086 ± 0.688
3.369IleLeu: 3.369 ± 0.42
1.915IleMet: 1.915 ± 0.356
4.029IleAsn: 4.029 ± 0.629
2.114IlePro: 2.114 ± 0.354
2.18IleGln: 2.18 ± 0.482
3.303IleArg: 3.303 ± 0.443
4.161IleSer: 4.161 ± 0.531
4.227IleThr: 4.227 ± 0.364
4.227IleVal: 4.227 ± 0.525
0.991IleTrp: 0.991 ± 0.295
2.246IleTyr: 2.246 ± 0.442
0.0IleXaa: 0.0 ± 0.0
Lys
6.209LysAla: 6.209 ± 0.939
1.255LysCys: 1.255 ± 0.299
4.095LysAsp: 4.095 ± 0.449
6.011LysGlu: 6.011 ± 0.555
3.038LysPhe: 3.038 ± 0.461
4.293LysGly: 4.293 ± 0.437
1.189LysHis: 1.189 ± 0.292
4.425LysIle: 4.425 ± 0.563
5.416LysLys: 5.416 ± 0.947
5.35LysLeu: 5.35 ± 0.59
3.104LysMet: 3.104 ± 0.46
3.17LysAsn: 3.17 ± 0.431
3.104LysPro: 3.104 ± 0.501
3.501LysGln: 3.501 ± 0.573
4.491LysArg: 4.491 ± 0.591
4.029LysSer: 4.029 ± 0.547
4.359LysThr: 4.359 ± 0.469
4.293LysVal: 4.293 ± 0.553
1.123LysTrp: 1.123 ± 0.307
2.312LysTyr: 2.312 ± 0.374
0.0LysXaa: 0.0 ± 0.0
Leu
4.954LeuAla: 4.954 ± 0.602
0.462LeuCys: 0.462 ± 0.212
4.293LeuAsp: 4.293 ± 0.538
3.765LeuGlu: 3.765 ± 0.683
1.849LeuPhe: 1.849 ± 0.31
4.227LeuGly: 4.227 ± 0.73
1.123LeuHis: 1.123 ± 0.235
4.227LeuIle: 4.227 ± 0.548
5.02LeuLys: 5.02 ± 0.672
3.765LeuLeu: 3.765 ± 0.494
1.915LeuMet: 1.915 ± 0.368
3.567LeuAsn: 3.567 ± 0.578
2.642LeuPro: 2.642 ± 0.458
2.048LeuGln: 2.048 ± 0.313
3.831LeuArg: 3.831 ± 0.436
3.831LeuSer: 3.831 ± 0.446
4.095LeuThr: 4.095 ± 0.493
4.425LeuVal: 4.425 ± 0.459
0.793LeuTrp: 0.793 ± 0.219
2.708LeuTyr: 2.708 ± 0.394
0.0LeuXaa: 0.0 ± 0.0
Met
3.501MetAla: 3.501 ± 0.396
0.462MetCys: 0.462 ± 0.193
1.387MetAsp: 1.387 ± 0.274
1.519MetGlu: 1.519 ± 0.335
0.925MetPhe: 0.925 ± 0.258
1.321MetGly: 1.321 ± 0.294
0.727MetHis: 0.727 ± 0.233
3.038MetIle: 3.038 ± 0.47
2.576MetLys: 2.576 ± 0.454
1.982MetLeu: 1.982 ± 0.36
0.859MetMet: 0.859 ± 0.224
1.321MetAsn: 1.321 ± 0.339
0.793MetPro: 0.793 ± 0.186
1.255MetGln: 1.255 ± 0.261
1.783MetArg: 1.783 ± 0.283
1.519MetSer: 1.519 ± 0.36
1.915MetThr: 1.915 ± 0.34
2.048MetVal: 2.048 ± 0.304
0.33MetTrp: 0.33 ± 0.172
0.859MetTyr: 0.859 ± 0.22
0.0MetXaa: 0.0 ± 0.0
Asn
4.095AsnAla: 4.095 ± 0.566
0.264AsnCys: 0.264 ± 0.143
3.104AsnAsp: 3.104 ± 0.473
3.567AsnGlu: 3.567 ± 0.446
1.717AsnPhe: 1.717 ± 0.297
5.548AsnGly: 5.548 ± 0.814
1.519AsnHis: 1.519 ± 0.323
3.104AsnIle: 3.104 ± 0.49
3.831AsnLys: 3.831 ± 0.51
2.642AsnLeu: 2.642 ± 0.383
1.123AsnMet: 1.123 ± 0.258
2.18AsnAsn: 2.18 ± 0.32
1.717AsnPro: 1.717 ± 0.363
1.982AsnGln: 1.982 ± 0.318
1.783AsnArg: 1.783 ± 0.319
3.236AsnSer: 3.236 ± 0.486
1.189AsnThr: 1.189 ± 0.214
3.369AsnVal: 3.369 ± 0.407
0.925AsnTrp: 0.925 ± 0.175
1.585AsnTyr: 1.585 ± 0.294
0.0AsnXaa: 0.0 ± 0.0
Pro
2.048ProAla: 2.048 ± 0.359
0.528ProCys: 0.528 ± 0.217
2.312ProAsp: 2.312 ± 0.519
2.906ProGlu: 2.906 ± 0.49
1.255ProPhe: 1.255 ± 0.268
2.642ProGly: 2.642 ± 0.44
0.462ProHis: 0.462 ± 0.147
1.915ProIle: 1.915 ± 0.401
1.255ProLys: 1.255 ± 0.242
1.519ProLeu: 1.519 ± 0.309
0.727ProMet: 0.727 ± 0.218
1.585ProAsn: 1.585 ± 0.29
1.585ProPro: 1.585 ± 0.465
1.321ProGln: 1.321 ± 0.259
1.321ProArg: 1.321 ± 0.324
1.519ProSer: 1.519 ± 0.297
1.651ProThr: 1.651 ± 0.277
4.029ProVal: 4.029 ± 0.444
0.33ProTrp: 0.33 ± 0.15
1.651ProTyr: 1.651 ± 0.299
0.0ProXaa: 0.0 ± 0.0
Gln
3.038GlnAla: 3.038 ± 0.448
0.528GlnCys: 0.528 ± 0.19
2.048GlnAsp: 2.048 ± 0.266
2.774GlnGlu: 2.774 ± 0.585
1.519GlnPhe: 1.519 ± 0.337
2.576GlnGly: 2.576 ± 0.451
0.859GlnHis: 0.859 ± 0.223
2.576GlnIle: 2.576 ± 0.457
2.576GlnLys: 2.576 ± 0.5
2.642GlnLeu: 2.642 ± 0.41
0.991GlnMet: 0.991 ± 0.235
1.519GlnAsn: 1.519 ± 0.312
1.123GlnPro: 1.123 ± 0.322
3.303GlnGln: 3.303 ± 0.639
1.982GlnArg: 1.982 ± 0.292
2.114GlnSer: 2.114 ± 0.425
1.519GlnThr: 1.519 ± 0.302
2.972GlnVal: 2.972 ± 0.38
0.661GlnTrp: 0.661 ± 0.258
1.982GlnTyr: 1.982 ± 0.323
0.0GlnXaa: 0.0 ± 0.0
Arg
4.425ArgAla: 4.425 ± 0.578
0.925ArgCys: 0.925 ± 0.336
3.567ArgAsp: 3.567 ± 0.562
3.633ArgGlu: 3.633 ± 0.418
2.708ArgPhe: 2.708 ± 0.394
2.84ArgGly: 2.84 ± 0.407
0.991ArgHis: 0.991 ± 0.245
3.699ArgIle: 3.699 ± 0.454
4.293ArgLys: 4.293 ± 0.634
3.236ArgLeu: 3.236 ± 0.676
1.453ArgMet: 1.453 ± 0.263
1.915ArgAsn: 1.915 ± 0.419
1.585ArgPro: 1.585 ± 0.357
1.849ArgGln: 1.849 ± 0.351
3.633ArgArg: 3.633 ± 0.513
3.104ArgSer: 3.104 ± 0.426
1.585ArgThr: 1.585 ± 0.325
4.756ArgVal: 4.756 ± 0.532
0.594ArgTrp: 0.594 ± 0.196
2.048ArgTyr: 2.048 ± 0.368
0.0ArgXaa: 0.0 ± 0.0
Ser
4.491SerAla: 4.491 ± 0.553
0.462SerCys: 0.462 ± 0.162
4.161SerAsp: 4.161 ± 0.524
4.359SerGlu: 4.359 ± 0.528
2.972SerPhe: 2.972 ± 0.358
5.548SerGly: 5.548 ± 0.511
1.057SerHis: 1.057 ± 0.247
4.491SerIle: 4.491 ± 0.653
4.425SerLys: 4.425 ± 0.472
4.095SerLeu: 4.095 ± 0.402
0.991SerMet: 0.991 ± 0.213
2.708SerAsn: 2.708 ± 0.413
1.915SerPro: 1.915 ± 0.263
2.378SerGln: 2.378 ± 0.363
3.038SerArg: 3.038 ± 0.39
3.369SerSer: 3.369 ± 0.486
2.708SerThr: 2.708 ± 0.452
4.029SerVal: 4.029 ± 0.407
1.057SerTrp: 1.057 ± 0.327
1.915SerTyr: 1.915 ± 0.355
0.0SerXaa: 0.0 ± 0.0
Thr
4.425ThrAla: 4.425 ± 0.657
0.528ThrCys: 0.528 ± 0.214
2.708ThrAsp: 2.708 ± 0.402
2.378ThrGlu: 2.378 ± 0.435
2.246ThrPhe: 2.246 ± 0.351
5.878ThrGly: 5.878 ± 0.773
0.396ThrHis: 0.396 ± 0.183
3.303ThrIle: 3.303 ± 0.505
3.104ThrLys: 3.104 ± 0.514
3.104ThrLeu: 3.104 ± 0.402
1.189ThrMet: 1.189 ± 0.235
2.774ThrAsn: 2.774 ± 0.431
2.246ThrPro: 2.246 ± 0.428
2.18ThrGln: 2.18 ± 0.41
1.585ThrArg: 1.585 ± 0.333
2.576ThrSer: 2.576 ± 0.407
1.915ThrThr: 1.915 ± 0.278
3.963ThrVal: 3.963 ± 0.484
0.661ThrTrp: 0.661 ± 0.196
2.114ThrTyr: 2.114 ± 0.389
0.0ThrXaa: 0.0 ± 0.0
Val
4.425ValAla: 4.425 ± 0.623
1.123ValCys: 1.123 ± 0.23
4.954ValAsp: 4.954 ± 0.495
5.416ValGlu: 5.416 ± 0.707
3.435ValPhe: 3.435 ± 0.51
3.963ValGly: 3.963 ± 0.468
1.321ValHis: 1.321 ± 0.325
5.152ValIle: 5.152 ± 0.552
6.341ValLys: 6.341 ± 0.648
4.227ValLeu: 4.227 ± 0.46
1.915ValMet: 1.915 ± 0.294
3.897ValAsn: 3.897 ± 0.473
2.444ValPro: 2.444 ± 0.47
2.576ValGln: 2.576 ± 0.563
3.831ValArg: 3.831 ± 0.442
5.02ValSer: 5.02 ± 0.538
4.227ValThr: 4.227 ± 0.496
5.02ValVal: 5.02 ± 0.634
1.387ValTrp: 1.387 ± 0.304
1.982ValTyr: 1.982 ± 0.379
0.0ValXaa: 0.0 ± 0.0
Trp
0.859TrpAla: 0.859 ± 0.252
0.396TrpCys: 0.396 ± 0.158
0.594TrpAsp: 0.594 ± 0.16
0.859TrpGlu: 0.859 ± 0.238
0.661TrpPhe: 0.661 ± 0.231
1.189TrpGly: 1.189 ± 0.239
0.727TrpHis: 0.727 ± 0.225
0.859TrpIle: 0.859 ± 0.231
1.255TrpLys: 1.255 ± 0.258
1.585TrpLeu: 1.585 ± 0.256
0.396TrpMet: 0.396 ± 0.162
0.594TrpAsn: 0.594 ± 0.199
0.462TrpPro: 0.462 ± 0.179
0.396TrpGln: 0.396 ± 0.137
1.255TrpArg: 1.255 ± 0.29
1.255TrpSer: 1.255 ± 0.267
1.123TrpThr: 1.123 ± 0.264
0.594TrpVal: 0.594 ± 0.212
0.264TrpTrp: 0.264 ± 0.133
0.528TrpTyr: 0.528 ± 0.151
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.104TyrAla: 3.104 ± 0.443
0.462TyrCys: 0.462 ± 0.143
2.576TyrAsp: 2.576 ± 0.448
2.774TyrGlu: 2.774 ± 0.41
1.387TyrPhe: 1.387 ± 0.325
2.642TyrGly: 2.642 ± 0.407
1.189TyrHis: 1.189 ± 0.276
1.783TyrIle: 1.783 ± 0.312
2.708TyrLys: 2.708 ± 0.477
1.915TyrLeu: 1.915 ± 0.36
1.387TyrMet: 1.387 ± 0.267
1.849TyrAsn: 1.849 ± 0.297
1.849TyrPro: 1.849 ± 0.337
1.849TyrGln: 1.849 ± 0.291
2.576TyrArg: 2.576 ± 0.399
2.312TyrSer: 2.312 ± 0.355
2.246TyrThr: 2.246 ± 0.332
2.378TyrVal: 2.378 ± 0.338
0.528TyrTrp: 0.528 ± 0.166
1.453TyrTyr: 1.453 ± 0.285
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 79 proteins (15141 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski