Marihabitans asiaticum
Average proteome isoelectric point is 5.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2675 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A560WEG0|A0A560WEG0_9MICO Transport permease protein OS=Marihabitans asiaticum OX=415218 GN=FB557_1604 PE=3 SV=1
MM1 pKa = 7.1 SHH3 pKa = 7.01 AGGPYY8 pKa = 10.29 GSDD11 pKa = 3.53 APPPPPPGQGAGGGYY26 pKa = 10.13 GGYY29 pKa = 9.13 PGPAGGPGEE38 pKa = 4.73 AGYY41 pKa = 10.51 GPPGDD46 pKa = 4.32 GGYY49 pKa = 10.52 GPPGGGYY56 pKa = 8.66 PPHH59 pKa = 6.97 VDD61 pKa = 3.79 GYY63 pKa = 11.46 GEE65 pKa = 4.04 QPPKK69 pKa = 10.51 KK70 pKa = 10.11 RR71 pKa = 11.84 NTLLIGALIGGALLLLLCCGGVVALGMMGSSGFDD105 pKa = 3.23 EE106 pKa = 5.53 APGEE110 pKa = 4.45 TTSATSEE117 pKa = 4.06 PSEE120 pKa = 4.48 SPTSSEE126 pKa = 4.1 TPTQSEE132 pKa = 5.06 TPTSSASPSEE142 pKa = 4.47 SEE144 pKa = 4.26 GSASEE149 pKa = 4.65 GEE151 pKa = 4.33 DD152 pKa = 3.75 LGFPDD157 pKa = 6.09 EE158 pKa = 4.16 FDD160 pKa = 2.6 GWQRR164 pKa = 11.84 SDD166 pKa = 3.48 QQASAPQGSQGAIYY180 pKa = 9.67 TKK182 pKa = 10.16 DD183 pKa = 3.19 DD184 pKa = 3.39 KK185 pKa = 11.6 AIVVVATDD193 pKa = 4.03 DD194 pKa = 4.1 APGAMDD200 pKa = 4.34 GFKK203 pKa = 10.14 MVWSDD208 pKa = 4.79 DD209 pKa = 3.52 KK210 pKa = 11.75 DD211 pKa = 3.68 VDD213 pKa = 3.92 GGVCGKK219 pKa = 9.89 IVSQTQCAATDD230 pKa = 3.86 DD231 pKa = 3.94 GTIFILNDD239 pKa = 3.15 TDD241 pKa = 4.73 GGDD244 pKa = 3.76 FEE246 pKa = 5.43 QVMGTLQAFLDD257 pKa = 3.94 ARR259 pKa = 4.09
Molecular weight: 25.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.69
IPC2_protein 3.719
IPC_protein 3.732
Toseland 3.516
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.668
Rodwell 3.554
Grimsley 3.414
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.088
Thurlkill 3.567
EMBOSS 3.681
Sillero 3.859
Patrickios 1.1
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.766
Protein with the highest isoelectric point:
>tr|A0A560WGI1|A0A560WGI1_9MICO CP family cyanate transporter-like MFS transporter OS=Marihabitans asiaticum OX=415218 GN=FB557_0329 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 SILANRR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.29 GRR40 pKa = 11.84 RR41 pKa = 11.84 KK42 pKa = 10.19 LSAA45 pKa = 3.66
Molecular weight: 5.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2675
0
2675
889342
29
3310
332.5
35.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.852 ± 0.065
0.7 ± 0.013
6.51 ± 0.042
6.351 ± 0.041
2.527 ± 0.029
9.396 ± 0.04
2.227 ± 0.024
3.6 ± 0.03
1.946 ± 0.037
10.117 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.918 ± 0.018
1.512 ± 0.02
5.553 ± 0.038
2.969 ± 0.023
7.755 ± 0.054
5.513 ± 0.034
5.959 ± 0.036
9.275 ± 0.046
1.49 ± 0.022
1.831 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here