Streptomyces aurantiacus JA 4570

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces aurantiacus group; Streptomyces aurantiacus group; Streptomyces aurantiacus

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7750 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S3ZUR5|S3ZUR5_9ACTN Uncharacterized protein OS=Streptomyces aurantiacus JA 4570 OX=1286094 GN=STRAU_0496 PE=4 SV=1
MM1 pKa = 7.24SADD4 pKa = 4.01VVWGGQWEE12 pKa = 4.47HH13 pKa = 6.53PVCGATGDD21 pKa = 4.2VVWDD25 pKa = 4.44DD26 pKa = 4.27EE27 pKa = 4.48DD28 pKa = 4.17TASSGHH34 pKa = 6.38DD35 pKa = 3.2CGGEE39 pKa = 4.32GEE41 pKa = 4.65VTWRR45 pKa = 11.84GAGAGVEE52 pKa = 4.08QFGDD56 pKa = 3.62DD57 pKa = 3.37TTTLSGHH64 pKa = 6.11DD65 pKa = 4.1CEE67 pKa = 4.59TAEE70 pKa = 5.52DD71 pKa = 3.79EE72 pKa = 4.67HH73 pKa = 8.88SDD75 pKa = 3.59GGVGEE80 pKa = 4.51VAAA83 pKa = 5.18

Molecular weight: 8.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S3ZD69|S3ZD69_9ACTN Putative HTH-type transcriptional activator TipA OS=Streptomyces aurantiacus JA 4570 OX=1286094 GN=STRAU_5856 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AVLASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.74GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7750

0

7750

2462931

30

7458

317.8

33.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.823 ± 0.04

0.808 ± 0.009

6.114 ± 0.026

5.657 ± 0.029

2.717 ± 0.016

9.735 ± 0.033

2.355 ± 0.018

2.963 ± 0.019

2.331 ± 0.026

10.221 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.731 ± 0.013

1.677 ± 0.014

6.164 ± 0.025

2.718 ± 0.019

8.213 ± 0.034

4.881 ± 0.018

5.924 ± 0.023

8.425 ± 0.03

1.494 ± 0.013

2.049 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski