Amino acid dipepetide frequency for Sphingomonas sp. Root241

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.606AlaAla: 19.606 ± 0.202	0.893AlaCys: 0.893 ± 0.031	7.224AlaAsp: 7.224 ± 0.083	7.871AlaGlu: 7.871 ± 0.125	4.35AlaPhe: 4.35 ± 0.072	12.146AlaGly: 12.146 ± 0.172	2.199AlaHis: 2.199 ± 0.046	6.609AlaIle: 6.609 ± 0.084	4.025AlaLys: 4.025 ± 0.07	14.02AlaLeu: 14.02 ± 0.162	3.579AlaMet: 3.579 ± 0.06	3.577AlaAsn: 3.577 ± 0.079	6.822AlaPro: 6.822 ± 0.098	4.499AlaGln: 4.499 ± 0.062	9.754AlaArg: 9.754 ± 0.112	6.71AlaSer: 6.71 ± 0.086	6.983AlaThr: 6.983 ± 0.099	8.556AlaVal: 8.556 ± 0.101	1.738AlaTrp: 1.738 ± 0.047	2.54AlaTyr: 2.54 ± 0.052	0.002AlaXaa: 0.002 ± 0.001
Cys	0.857CysAla: 0.857 ± 0.032	0.075CysCys: 0.075 ± 0.008	0.422CysAsp: 0.422 ± 0.018	0.363CysGlu: 0.363 ± 0.018	0.252CysPhe: 0.252 ± 0.015	0.748CysGly: 0.748 ± 0.027	0.154CysHis: 0.154 ± 0.012	0.308CysIle: 0.308 ± 0.014	0.157CysLys: 0.157 ± 0.011	0.582CysLeu: 0.582 ± 0.021	0.124CysMet: 0.124 ± 0.01	0.179CysAsn: 0.179 ± 0.013	0.361CysPro: 0.361 ± 0.017	0.149CysGln: 0.149 ± 0.012	0.493CysArg: 0.493 ± 0.021	0.356CysSer: 0.356 ± 0.017	0.389CysThr: 0.389 ± 0.02	0.492CysVal: 0.492 ± 0.02	0.102CysTrp: 0.102 ± 0.009	0.139CysTyr: 0.139 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.847AspAla: 7.847 ± 0.08	0.422AspCys: 0.422 ± 0.02	3.022AspAsp: 3.022 ± 0.058	3.099AspGlu: 3.099 ± 0.057	2.261AspPhe: 2.261 ± 0.048	5.423AspGly: 5.423 ± 0.097	1.118AspHis: 1.118 ± 0.036	2.695AspIle: 2.695 ± 0.051	1.656AspLys: 1.656 ± 0.041	5.543AspLeu: 5.543 ± 0.08	1.243AspMet: 1.243 ± 0.033	1.371AspAsn: 1.371 ± 0.045	3.925AspPro: 3.925 ± 0.064	1.73AspGln: 1.73 ± 0.04	4.623AspArg: 4.623 ± 0.07	2.446AspSer: 2.446 ± 0.043	2.701AspThr: 2.701 ± 0.065	3.962AspVal: 3.962 ± 0.06	1.171AspTrp: 1.171 ± 0.031	1.569AspTyr: 1.569 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	7.815GluAla: 7.815 ± 0.119	0.292GluCys: 0.292 ± 0.016	2.557GluAsp: 2.557 ± 0.053	2.855GluGlu: 2.855 ± 0.067	1.558GluPhe: 1.558 ± 0.04	4.628GluGly: 4.628 ± 0.077	1.098GluHis: 1.098 ± 0.032	2.995GluIle: 2.995 ± 0.054	1.914GluLys: 1.914 ± 0.045	5.041GluLeu: 5.041 ± 0.084	1.373GluMet: 1.373 ± 0.031	1.345GluAsn: 1.345 ± 0.035	2.617GluPro: 2.617 ± 0.055	1.955GluGln: 1.955 ± 0.045	4.842GluArg: 4.842 ± 0.078	2.343GluSer: 2.343 ± 0.049	3.236GluThr: 3.236 ± 0.059	3.609GluVal: 3.609 ± 0.062	0.799GluTrp: 0.799 ± 0.028	1.005GluTyr: 1.005 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	4.965PheAla: 4.965 ± 0.071	0.271PheCys: 0.271 ± 0.015	2.77PheAsp: 2.77 ± 0.052	2.145PheGlu: 2.145 ± 0.044	1.279PhePhe: 1.279 ± 0.042	3.767PheGly: 3.767 ± 0.051	0.766PheHis: 0.766 ± 0.03	1.238PheIle: 1.238 ± 0.031	0.922PheLys: 0.922 ± 0.024	3.213PheLeu: 3.213 ± 0.068	0.669PheMet: 0.669 ± 0.024	1.135PheAsn: 1.135 ± 0.037	1.504PhePro: 1.504 ± 0.034	0.977PheGln: 0.977 ± 0.029	2.308PheArg: 2.308 ± 0.05	1.941PheSer: 1.941 ± 0.046	2.165PheThr: 2.165 ± 0.047	2.742PheVal: 2.742 ± 0.055	0.554PheTrp: 0.554 ± 0.023	0.939PheTyr: 0.939 ± 0.032	0.0PheXaa: 0.0 ± 0.0
Gly	10.647GlyAla: 10.647 ± 0.18	0.762GlyCys: 0.762 ± 0.027	5.112GlyAsp: 5.112 ± 0.085	4.971GlyGlu: 4.971 ± 0.068	3.811GlyPhe: 3.811 ± 0.065	8.837GlyGly: 8.837 ± 0.217	1.744GlyHis: 1.744 ± 0.042	4.466GlyIle: 4.466 ± 0.091	3.391GlyLys: 3.391 ± 0.062	8.648GlyLeu: 8.648 ± 0.098	2.207GlyMet: 2.207 ± 0.05	2.764GlyAsn: 2.764 ± 0.106	3.799GlyPro: 3.799 ± 0.058	2.995GlyGln: 2.995 ± 0.055	6.382GlyArg: 6.382 ± 0.077	5.298GlySer: 5.298 ± 0.127	5.767GlyThr: 5.767 ± 0.25	6.578GlyVal: 6.578 ± 0.08	1.746GlyTrp: 1.746 ± 0.043	2.463GlyTyr: 2.463 ± 0.045	0.001GlyXaa: 0.001 ± 0.001
His	2.401HisAla: 2.401 ± 0.051	0.169HisCys: 0.169 ± 0.012	1.113HisAsp: 1.113 ± 0.031	0.908HisGlu: 0.908 ± 0.027	0.82HisPhe: 0.82 ± 0.028	1.835HisGly: 1.835 ± 0.043	0.495HisHis: 0.495 ± 0.022	0.833HisIle: 0.833 ± 0.026	0.447HisLys: 0.447 ± 0.022	1.739HisLeu: 1.739 ± 0.036	0.417HisMet: 0.417 ± 0.019	0.48HisAsn: 0.48 ± 0.019	1.192HisPro: 1.192 ± 0.031	0.523HisGln: 0.523 ± 0.02	1.404HisArg: 1.404 ± 0.036	0.86HisSer: 0.86 ± 0.026	0.648HisThr: 0.648 ± 0.021	1.413HisVal: 1.413 ± 0.03	0.365HisTrp: 0.365 ± 0.016	0.561HisTyr: 0.561 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	7.639IleAla: 7.639 ± 0.088	0.32IleCys: 0.32 ± 0.017	3.719IleAsp: 3.719 ± 0.061	3.499IleGlu: 3.499 ± 0.063	1.481IlePhe: 1.481 ± 0.038	5.085IleGly: 5.085 ± 0.075	0.796IleHis: 0.796 ± 0.032	1.562IleIle: 1.562 ± 0.043	1.122IleLys: 1.122 ± 0.031	3.936IleLeu: 3.936 ± 0.059	0.67IleMet: 0.67 ± 0.027	1.382IleAsn: 1.382 ± 0.048	2.155IlePro: 2.155 ± 0.042	1.216IleGln: 1.216 ± 0.032	3.209IleArg: 3.209 ± 0.049	2.397IleSer: 2.397 ± 0.047	2.599IleThr: 2.599 ± 0.061	4.023IleVal: 4.023 ± 0.063	0.591IleTrp: 0.591 ± 0.021	0.957IleTyr: 0.957 ± 0.028	0.0IleXaa: 0.0 ± 0.0
Lys	3.835LysAla: 3.835 ± 0.066	0.138LysCys: 0.138 ± 0.01	1.491LysAsp: 1.491 ± 0.038	1.148LysGlu: 1.148 ± 0.037	0.895LysPhe: 0.895 ± 0.031	2.59LysGly: 2.59 ± 0.054	0.532LysHis: 0.532 ± 0.02	1.374LysIle: 1.374 ± 0.043	0.994LysLys: 0.994 ± 0.037	3.306LysLeu: 3.306 ± 0.065	0.722LysMet: 0.722 ± 0.023	0.739LysAsn: 0.739 ± 0.028	2.11LysPro: 2.11 ± 0.054	0.866LysGln: 0.866 ± 0.029	2.132LysArg: 2.132 ± 0.043	1.481LysSer: 1.481 ± 0.041	1.614LysThr: 1.614 ± 0.039	2.092LysVal: 2.092 ± 0.049	0.389LysTrp: 0.389 ± 0.016	0.617LysTyr: 0.617 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	14.328LeuAla: 14.328 ± 0.149	0.693LeuCys: 0.693 ± 0.023	6.193LeuAsp: 6.193 ± 0.087	4.753LeuGlu: 4.753 ± 0.08	3.579LeuPhe: 3.579 ± 0.071	8.756LeuGly: 8.756 ± 0.11	1.755LeuHis: 1.755 ± 0.043	4.72LeuIle: 4.72 ± 0.077	2.907LeuLys: 2.907 ± 0.059	9.512LeuLeu: 9.512 ± 0.141	1.929LeuMet: 1.929 ± 0.046	2.556LeuAsn: 2.556 ± 0.067	5.746LeuPro: 5.746 ± 0.087	2.631LeuGln: 2.631 ± 0.054	6.885LeuArg: 6.885 ± 0.09	5.612LeuSer: 5.612 ± 0.072	5.633LeuThr: 5.633 ± 0.107	7.244LeuVal: 7.244 ± 0.087	1.301LeuTrp: 1.301 ± 0.037	2.014LeuTyr: 2.014 ± 0.041	0.0LeuXaa: 0.0 ± 0.0
Met	2.906MetAla: 2.906 ± 0.057	0.126MetCys: 0.126 ± 0.01	0.984MetAsp: 0.984 ± 0.028	0.922MetGlu: 0.922 ± 0.028	0.662MetPhe: 0.662 ± 0.025	1.742MetGly: 1.742 ± 0.042	0.426MetHis: 0.426 ± 0.018	1.253MetIle: 1.253 ± 0.034	0.809MetLys: 0.809 ± 0.028	2.53MetLeu: 2.53 ± 0.051	0.536MetMet: 0.536 ± 0.022	0.606MetAsn: 0.606 ± 0.021	1.406MetPro: 1.406 ± 0.034	0.717MetGln: 0.717 ± 0.026	1.778MetArg: 1.778 ± 0.039	1.24MetSer: 1.24 ± 0.031	1.52MetThr: 1.52 ± 0.035	1.516MetVal: 1.516 ± 0.033	0.226MetTrp: 0.226 ± 0.013	0.251MetTyr: 0.251 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	3.434AsnAla: 3.434 ± 0.077	0.234AsnCys: 0.234 ± 0.016	1.448AsnAsp: 1.448 ± 0.054	1.11AsnGlu: 1.11 ± 0.032	1.098AsnPhe: 1.098 ± 0.038	2.939AsnGly: 2.939 ± 0.106	0.466AsnHis: 0.466 ± 0.02	1.346AsnIle: 1.346 ± 0.034	0.639AsnLys: 0.639 ± 0.023	2.733AsnLeu: 2.733 ± 0.071	0.528AsnMet: 0.528 ± 0.02	0.89AsnAsn: 0.89 ± 0.044	1.894AsnPro: 1.894 ± 0.041	0.821AsnGln: 0.821 ± 0.03	1.857AsnArg: 1.857 ± 0.041	1.395AsnSer: 1.395 ± 0.056	1.326AsnThr: 1.326 ± 0.069	2.161AsnVal: 2.161 ± 0.058	0.498AsnTrp: 0.498 ± 0.019	0.835AsnTyr: 0.835 ± 0.035	0.0AsnXaa: 0.0 ± 0.0
Pro	7.46ProAla: 7.46 ± 0.098	0.292ProCys: 0.292 ± 0.015	3.612ProAsp: 3.612 ± 0.056	3.75ProGlu: 3.75 ± 0.066	1.972ProPhe: 1.972 ± 0.04	5.23ProGly: 5.23 ± 0.074	0.991ProHis: 0.991 ± 0.031	2.401ProIle: 2.401 ± 0.048	1.495ProLys: 1.495 ± 0.036	5.021ProLeu: 5.021 ± 0.078	1.123ProMet: 1.123 ± 0.033	1.444ProAsn: 1.444 ± 0.034	2.749ProPro: 2.749 ± 0.069	1.722ProGln: 1.722 ± 0.039	3.196ProArg: 3.196 ± 0.052	2.762ProSer: 2.762 ± 0.054	2.597ProThr: 2.597 ± 0.049	4.346ProVal: 4.346 ± 0.065	0.731ProTrp: 0.731 ± 0.027	1.094ProTyr: 1.094 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	4.072GlnAla: 4.072 ± 0.064	0.189GlnCys: 0.189 ± 0.013	1.368GlnAsp: 1.368 ± 0.035	1.265GlnGlu: 1.265 ± 0.037	1.134GlnPhe: 1.134 ± 0.031	2.643GlnGly: 2.643 ± 0.042	0.574GlnHis: 0.574 ± 0.021	1.616GlnIle: 1.616 ± 0.036	0.846GlnLys: 0.846 ± 0.026	3.249GlnLeu: 3.249 ± 0.051	0.8GlnMet: 0.8 ± 0.029	0.817GlnAsn: 0.817 ± 0.029	1.829GlnPro: 1.829 ± 0.045	1.139GlnGln: 1.139 ± 0.036	2.473GlnArg: 2.473 ± 0.05	1.68GlnSer: 1.68 ± 0.039	1.738GlnThr: 1.738 ± 0.045	2.342GlnVal: 2.342 ± 0.048	0.432GlnTrp: 0.432 ± 0.02	0.623GlnTyr: 0.623 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	8.847ArgAla: 8.847 ± 0.125	0.412ArgCys: 0.412 ± 0.019	4.206ArgAsp: 4.206 ± 0.061	4.006ArgGlu: 4.006 ± 0.063	3.188ArgPhe: 3.188 ± 0.056	5.384ArgGly: 5.384 ± 0.074	1.585ArgHis: 1.585 ± 0.036	4.102ArgIle: 4.102 ± 0.071	2.009ArgLys: 2.009 ± 0.041	7.934ArgLeu: 7.934 ± 0.11	1.876ArgMet: 1.876 ± 0.045	1.888ArgAsn: 1.888 ± 0.039	3.704ArgPro: 3.704 ± 0.055	2.323ArgGln: 2.323 ± 0.045	5.602ArgArg: 5.602 ± 0.085	3.617ArgSer: 3.617 ± 0.054	3.713ArgThr: 3.713 ± 0.057	5.07ArgVal: 5.07 ± 0.07	1.238ArgTrp: 1.238 ± 0.039	1.902ArgTyr: 1.902 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	6.348SerAla: 6.348 ± 0.08	0.325SerCys: 0.325 ± 0.021	3.037SerAsp: 3.037 ± 0.05	2.558SerGlu: 2.558 ± 0.055	2.298SerPhe: 2.298 ± 0.045	5.836SerGly: 5.836 ± 0.128	0.938SerHis: 0.938 ± 0.029	2.689SerIle: 2.689 ± 0.053	1.382SerLys: 1.382 ± 0.034	4.969SerLeu: 4.969 ± 0.064	1.093SerMet: 1.093 ± 0.027	1.503SerAsn: 1.503 ± 0.053	2.78SerPro: 2.78 ± 0.05	1.555SerGln: 1.555 ± 0.037	3.294SerArg: 3.294 ± 0.059	2.677SerSer: 2.677 ± 0.061	2.808SerThr: 2.808 ± 0.061	3.72SerVal: 3.72 ± 0.06	0.863SerTrp: 0.863 ± 0.026	1.428SerTyr: 1.428 ± 0.052	0.001SerXaa: 0.001 ± 0.001
Thr	6.773ThrAla: 6.773 ± 0.098	0.349ThrCys: 0.349 ± 0.017	2.927ThrAsp: 2.927 ± 0.051	2.41ThrGlu: 2.41 ± 0.042	1.873ThrPhe: 1.873 ± 0.04	6.075ThrGly: 6.075 ± 0.182	0.91ThrHis: 0.91 ± 0.026	3.192ThrIle: 3.192 ± 0.084	1.27ThrLys: 1.27 ± 0.032	6.186ThrLeu: 6.186 ± 0.14	1.081ThrMet: 1.081 ± 0.028	1.546ThrAsn: 1.546 ± 0.06	3.621ThrPro: 3.621 ± 0.063	1.511ThrGln: 1.511 ± 0.038	3.647ThrArg: 3.647 ± 0.061	2.869ThrSer: 2.869 ± 0.065	3.117ThrThr: 3.117 ± 0.098	4.36ThrVal: 4.36 ± 0.094	0.633ThrTrp: 0.633 ± 0.023	1.233ThrTyr: 1.233 ± 0.036	0.003ThrXaa: 0.003 ± 0.002
Val	9.644ValAla: 9.644 ± 0.116	0.432ValCys: 0.432 ± 0.02	4.274ValAsp: 4.274 ± 0.065	4.445ValGlu: 4.445 ± 0.068	2.256ValPhe: 2.256 ± 0.04	5.527ValGly: 5.527 ± 0.073	1.296ValHis: 1.296 ± 0.036	3.483ValIle: 3.483 ± 0.058	1.995ValLys: 1.995 ± 0.052	6.74ValLeu: 6.74 ± 0.096	1.46ValMet: 1.46 ± 0.037	2.236ValAsn: 2.236 ± 0.067	4.011ValPro: 4.011 ± 0.058	2.139ValGln: 2.139 ± 0.042	5.332ValArg: 5.332 ± 0.08	4.186ValSer: 4.186 ± 0.06	5.006ValThr: 5.006 ± 0.091	5.282ValVal: 5.282 ± 0.077	0.824ValTrp: 0.824 ± 0.027	1.427ValTyr: 1.427 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	1.469TrpAla: 1.469 ± 0.036	0.125TrpCys: 0.125 ± 0.009	0.752TrpAsp: 0.752 ± 0.025	0.655TrpGlu: 0.655 ± 0.025	0.603TrpPhe: 0.603 ± 0.024	1.016TrpGly: 1.016 ± 0.029	0.351TrpHis: 0.351 ± 0.017	0.693TrpIle: 0.693 ± 0.024	0.51TrpLys: 0.51 ± 0.024	1.768TrpLeu: 1.768 ± 0.044	0.352TrpMet: 0.352 ± 0.017	0.499TrpAsn: 0.499 ± 0.024	0.722TrpPro: 0.722 ± 0.024	0.624TrpGln: 0.624 ± 0.024	1.393TrpArg: 1.393 ± 0.04	0.939TrpSer: 0.939 ± 0.03	0.867TrpThr: 0.867 ± 0.028	0.865TrpVal: 0.865 ± 0.032	0.308TrpTrp: 0.308 ± 0.018	0.37TrpTyr: 0.37 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.812TyrAla: 2.812 ± 0.053	0.181TyrCys: 0.181 ± 0.011	1.598TyrAsp: 1.598 ± 0.055	1.144TyrGlu: 1.144 ± 0.03	0.899TyrPhe: 0.899 ± 0.034	2.185TyrGly: 2.185 ± 0.045	0.428TyrHis: 0.428 ± 0.018	0.785TyrIle: 0.785 ± 0.024	0.575TyrLys: 0.575 ± 0.022	2.076TyrLeu: 2.076 ± 0.047	0.373TyrMet: 0.373 ± 0.017	0.695TyrAsn: 0.695 ± 0.032	1.041TyrPro: 1.041 ± 0.029	0.739TyrGln: 0.739 ± 0.023	1.999TyrArg: 1.999 ± 0.045	1.289TyrSer: 1.289 ± 0.042	1.159TyrThr: 1.159 ± 0.045	1.619TyrVal: 1.619 ± 0.04	0.37TyrTrp: 0.37 ± 0.016	0.665TyrTyr: 0.665 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.001	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3715 proteins (1245473 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---