Bat polyomavirus 6d
Average proteome isoelectric point is 5.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D5ZYQ7|A0A0D5ZYQ7_9POLY Small t antigen OS=Bat polyomavirus 6d OX=1623686 PE=4 SV=1
MM1 pKa = 7.31 GALLAVLAEE10 pKa = 4.54 VIDD13 pKa = 4.36 LSAVTGISVEE23 pKa = 4.37 GILAGEE29 pKa = 4.48 AFATAEE35 pKa = 3.94 VLAAQIEE42 pKa = 4.75 NITLLEE48 pKa = 4.19 GLTAAEE54 pKa = 4.39 AVAEE58 pKa = 4.11 LGYY61 pKa = 9.24 TVDD64 pKa = 3.54 VFNALASVTEE74 pKa = 4.05 NFPEE78 pKa = 4.45 VFTLLAAGEE87 pKa = 4.27 VAANSSLVLSAAVAAATYY105 pKa = 8.63 PYY107 pKa = 10.09 SYY109 pKa = 9.28 THH111 pKa = 6.44 EE112 pKa = 4.19 VPIANLNNMALQVWQPEE129 pKa = 3.73 IDD131 pKa = 3.34 ILFPGISSLARR142 pKa = 11.84 FLNYY146 pKa = 9.33 IDD148 pKa = 3.62 PTNWAGDD155 pKa = 3.49 LFRR158 pKa = 11.84 AVGRR162 pKa = 11.84 YY163 pKa = 7.21 FWEE166 pKa = 4.06 TAQRR170 pKa = 11.84 SGRR173 pKa = 11.84 NLLEE177 pKa = 3.66 QEE179 pKa = 3.65 IRR181 pKa = 11.84 AVGQRR186 pKa = 11.84 AAGGFSEE193 pKa = 4.2 TLARR197 pKa = 11.84 YY198 pKa = 9.21 FEE200 pKa = 4.05 NARR203 pKa = 11.84 WAIRR207 pKa = 11.84 HH208 pKa = 5.4 YY209 pKa = 10.56 PADD212 pKa = 3.66 LYY214 pKa = 11.58 GRR216 pKa = 11.84 LQQYY220 pKa = 7.71 YY221 pKa = 10.04 TDD223 pKa = 5.11 LPPLNPAQLRR233 pKa = 11.84 EE234 pKa = 3.78 LSRR237 pKa = 11.84 RR238 pKa = 11.84 VGGPDD243 pKa = 3.42 QPYY246 pKa = 10.53 KK247 pKa = 10.94 LYY249 pKa = 10.67 DD250 pKa = 3.51 SHH252 pKa = 7.94 VKK254 pKa = 10.17 SAEE257 pKa = 4.05 FVTKK261 pKa = 10.51 SPPPGGAHH269 pKa = 6.18 QRR271 pKa = 11.84 TTPDD275 pKa = 2.65 WLLPLILGLYY285 pKa = 10.37 GDD287 pKa = 5.25 ISPSWADD294 pKa = 3.23 TLEE297 pKa = 4.06 EE298 pKa = 4.48 LEE300 pKa = 4.54 EE301 pKa = 4.54 EE302 pKa = 4.01 EE303 pKa = 5.96 DD304 pKa = 4.02 GPKK307 pKa = 9.87 KK308 pKa = 10.26 KK309 pKa = 10.08 KK310 pKa = 9.9 RR311 pKa = 11.84 ASQRR315 pKa = 11.84 SSRR318 pKa = 11.84 SAKK321 pKa = 9.27 TNNN324 pKa = 3.08
Molecular weight: 35.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.813
IPC2_protein 4.851
IPC_protein 4.762
Toseland 4.647
ProMoST 4.851
Dawson 4.698
Bjellqvist 4.851
Wikipedia 4.546
Rodwell 4.622
Grimsley 4.558
Solomon 4.698
Lehninger 4.647
Nozaki 4.813
DTASelect 4.914
Thurlkill 4.635
EMBOSS 4.571
Sillero 4.889
Patrickios 3.884
IPC_peptide 4.711
IPC2_peptide 4.876
IPC2.peptide.svr19 4.859
Protein with the highest isoelectric point:
>tr|A0A0D5ZYQ7|A0A0D5ZYQ7_9POLY Small t antigen OS=Bat polyomavirus 6d OX=1623686 PE=4 SV=1
MM1 pKa = 7.77 DD2 pKa = 5.3 HH3 pKa = 7.29 LLTRR7 pKa = 11.84 EE8 pKa = 3.66 EE9 pKa = 4.76 SIRR12 pKa = 11.84 LMQLLEE18 pKa = 4.24 LPMDD22 pKa = 3.65 EE23 pKa = 4.77 FGNFNAMRR31 pKa = 11.84 SQFHH35 pKa = 5.6 KK36 pKa = 10.25 QIKK39 pKa = 9.53 KK40 pKa = 6.86 MHH42 pKa = 6.94 PDD44 pKa = 2.82 KK45 pKa = 11.35 GGNPEE50 pKa = 3.83 QAKK53 pKa = 10.2 EE54 pKa = 4.03 LISLYY59 pKa = 10.69 KK60 pKa = 10.38 KK61 pKa = 10.66 LEE63 pKa = 4.13 EE64 pKa = 5.23 KK65 pKa = 10.38 ISPLQPDD72 pKa = 3.25 SDD74 pKa = 3.82 ATTTEE79 pKa = 3.71 QVYY82 pKa = 9.71 EE83 pKa = 3.87 QGQFFLYY90 pKa = 10.6 LKK92 pKa = 10.57 DD93 pKa = 3.18 WVSCNRR99 pKa = 11.84 GEE101 pKa = 4.54 SSCACLFCMLKK112 pKa = 10.7 ANHH115 pKa = 6.24 EE116 pKa = 4.02 KK117 pKa = 10.46 RR118 pKa = 11.84 KK119 pKa = 10.0 RR120 pKa = 11.84 EE121 pKa = 3.83 RR122 pKa = 11.84 KK123 pKa = 8.42 HH124 pKa = 6.19 NVWGHH129 pKa = 5.65 CFCFQCYY136 pKa = 7.7 ITWFGLEE143 pKa = 4.33 HH144 pKa = 7.39 SWMDD148 pKa = 3.13 WLSWRR153 pKa = 11.84 NVIANTPYY161 pKa = 10.73 GCLNII166 pKa = 4.6
Molecular weight: 19.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.439
IPC2_protein 6.453
IPC_protein 6.59
Toseland 6.561
ProMoST 7.044
Dawson 7.088
Bjellqvist 7.029
Wikipedia 7.059
Rodwell 7.088
Grimsley 6.649
Solomon 7.117
Lehninger 7.132
Nozaki 7.395
DTASelect 7.395
Thurlkill 7.424
EMBOSS 7.439
Sillero 7.541
Patrickios 4.037
IPC_peptide 7.132
IPC2_peptide 7.117
IPC2.peptide.svr19 6.962
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1518
166
661
379.5
42.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.588 ± 1.983
2.306 ± 0.886
5.468 ± 0.609
7.642 ± 0.388
4.875 ± 0.644
5.863 ± 0.977
1.845 ± 0.463
4.677 ± 0.175
6.522 ± 1.349
10.738 ± 0.498
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.174 ± 0.535
4.875 ± 0.33
5.138 ± 0.83
4.414 ± 0.456
4.611 ± 0.299
5.863 ± 0.225
6.258 ± 0.623
5.402 ± 0.707
1.383 ± 0.486
3.36 ± 0.317
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here