Pseudomonas yangmingensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2926 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I4TAS2|A0A1I4TAS2_9PSED Uncharacterized protein OS=Pseudomonas yangmingensis OX=1720063 GN=SAMN05216217_11398 PE=4 SV=1
MM1 pKa = 7.66SSLPPLTLYY10 pKa = 8.37GTLACHH16 pKa = 7.02LCDD19 pKa = 4.5AALQVLQPLLADD31 pKa = 4.3GLQVQEE37 pKa = 4.13VDD39 pKa = 3.1ISEE42 pKa = 4.35DD43 pKa = 3.35AQLLEE48 pKa = 4.8RR49 pKa = 11.84YY50 pKa = 7.46QLRR53 pKa = 11.84IPVLRR58 pKa = 11.84RR59 pKa = 11.84MDD61 pKa = 3.39TGAEE65 pKa = 3.78LDD67 pKa = 4.17FPFDD71 pKa = 4.81LPALMDD77 pKa = 3.98WLADD81 pKa = 3.82CLQEE85 pKa = 4.54GAA87 pKa = 4.75

Molecular weight: 9.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4R188|A0A1I4R188_9PSED Diguanylate cyclase (GGDEF) domain-containing protein OS=Pseudomonas yangmingensis OX=1720063 GN=SAMN05216217_105159 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.37RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.25GRR39 pKa = 11.84QRR41 pKa = 11.84LSAA44 pKa = 4.04

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2926

0

2926

953707

25

3027

325.9

35.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.674 ± 0.049

1.057 ± 0.016

5.49 ± 0.029

5.963 ± 0.045

3.372 ± 0.029

7.792 ± 0.042

2.317 ± 0.022

4.769 ± 0.038

2.844 ± 0.043

12.477 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.411 ± 0.022

2.911 ± 0.029

4.788 ± 0.03

5.311 ± 0.052

7.176 ± 0.044

5.719 ± 0.029

4.389 ± 0.032

6.769 ± 0.042

1.463 ± 0.02

2.308 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski