Seonamhaeicola aphaedonensis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Seonamhaeicola

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2965 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3D9H939|A0A3D9H939_9FLAO Phytoene desaturase OS=Seonamhaeicola aphaedonensis OX=1461338 GN=DFQ02_107137 PE=3 SV=1
MM1 pKa = 7.38KK2 pKa = 10.27LEE4 pKa = 4.57KK5 pKa = 10.06IGLFALCMVFCFLSCNKK22 pKa = 9.71NDD24 pKa = 3.74EE25 pKa = 4.5ASDD28 pKa = 3.65DD29 pKa = 4.24VIVVEE34 pKa = 4.37IRR36 pKa = 11.84DD37 pKa = 3.65RR38 pKa = 11.84YY39 pKa = 7.95EE40 pKa = 3.43QQIIDD45 pKa = 3.8NDD47 pKa = 4.05SLVEE51 pKa = 3.96FLEE54 pKa = 3.83THH56 pKa = 6.64YY57 pKa = 10.88YY58 pKa = 9.62NKK60 pKa = 10.48SAFEE64 pKa = 4.39GNSNPKK70 pKa = 8.53ITDD73 pKa = 3.53LQILSTQGLSISEE86 pKa = 4.26NADD89 pKa = 3.22SLLINAVGTSKK100 pKa = 9.53KK101 pKa = 8.93TVFADD106 pKa = 3.36ANYY109 pKa = 10.5EE110 pKa = 4.08YY111 pKa = 11.3YY112 pKa = 10.02ILNLNNGGGAEE123 pKa = 4.32SPTFADD129 pKa = 3.94NVLATYY135 pKa = 9.97EE136 pKa = 4.83GYY138 pKa = 8.38TLDD141 pKa = 5.1ADD143 pKa = 5.33LNNLDD148 pKa = 4.74DD149 pKa = 5.48AFDD152 pKa = 4.01SKK154 pKa = 11.66VNPDD158 pKa = 3.32TYY160 pKa = 11.45FDD162 pKa = 4.25LTTLIPGWRR171 pKa = 11.84RR172 pKa = 11.84VFPQFNVAAGYY183 pKa = 10.27VDD185 pKa = 4.7NGDD188 pKa = 3.63GTISFSNPGIGVMFLPSGLGYY209 pKa = 9.85YY210 pKa = 10.42QNAQSLIPAYY220 pKa = 10.57SPLIFKK226 pKa = 10.67FEE228 pKa = 5.06LIATTEE234 pKa = 3.96NDD236 pKa = 3.59HH237 pKa = 7.39DD238 pKa = 4.23NDD240 pKa = 5.23GIPSHH245 pKa = 7.4KK246 pKa = 10.21EE247 pKa = 3.6DD248 pKa = 5.17LNDD251 pKa = 5.52DD252 pKa = 3.52GDD254 pKa = 4.33FNINSAIGVIDD265 pKa = 4.9GDD267 pKa = 4.13DD268 pKa = 3.65TDD270 pKa = 4.67EE271 pKa = 6.0DD272 pKa = 4.88GIPNYY277 pKa = 10.04FDD279 pKa = 5.62PDD281 pKa = 4.0DD282 pKa = 5.68DD283 pKa = 5.88GDD285 pKa = 4.8GIPTANEE292 pKa = 4.84DD293 pKa = 3.25IDD295 pKa = 5.58GDD297 pKa = 4.05GDD299 pKa = 3.69PTNDD303 pKa = 2.9IGKK306 pKa = 9.68NGIAKK311 pKa = 10.23YY312 pKa = 10.29LDD314 pKa = 3.81PEE316 pKa = 4.35EE317 pKa = 4.67TEE319 pKa = 4.58SNN321 pKa = 3.47

Molecular weight: 35.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3D9H1K3|A0A3D9H1K3_9FLAO Uncharacterized protein OS=Seonamhaeicola aphaedonensis OX=1461338 GN=DFQ02_1382 PE=4 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.32KK6 pKa = 9.59RR7 pKa = 11.84KK8 pKa = 7.05RR9 pKa = 11.84HH10 pKa = 5.11KK11 pKa = 10.59VATHH15 pKa = 5.21KK16 pKa = 10.3RR17 pKa = 11.84KK18 pKa = 9.62KK19 pKa = 9.04RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84ANRR25 pKa = 11.84HH26 pKa = 4.77KK27 pKa = 10.58KK28 pKa = 9.8KK29 pKa = 10.51KK30 pKa = 10.04

Molecular weight: 3.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2965

0

2965

1052557

30

3606

355.0

40.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.03 ± 0.044

0.793 ± 0.015

5.67 ± 0.039

6.535 ± 0.046

5.283 ± 0.038

6.356 ± 0.04

1.858 ± 0.023

8.034 ± 0.045

7.988 ± 0.063

9.184 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.153 ± 0.023

6.448 ± 0.053

3.418 ± 0.026

3.229 ± 0.025

3.407 ± 0.032

6.459 ± 0.043

5.647 ± 0.042

6.199 ± 0.035

1.132 ± 0.022

4.177 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski