Poophage MBI-2016a

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P9FIV2|A0A2P9FIV2_9VIRU Putative replication protein OS=Poophage MBI-2016a OX=1926504 PE=4 SV=1
MM1 pKa = 8.11VEE3 pKa = 3.88VGLSILLQQVVNFVVNNLYY22 pKa = 10.9LIFLCLYY29 pKa = 10.23LPLNFKK35 pKa = 10.48SYY37 pKa = 11.27SIMLSNVISEE47 pKa = 4.72DD48 pKa = 3.5KK49 pKa = 10.53EE50 pKa = 4.11KK51 pKa = 11.29SSVSGSDD58 pKa = 3.43SIISVLISNYY68 pKa = 8.84PLSNGGYY75 pKa = 9.45LVSFGQDD82 pKa = 2.93QPDD85 pKa = 3.36GSFKK89 pKa = 11.01HH90 pKa = 5.96FDD92 pKa = 3.73PVSSLDD98 pKa = 3.66FEE100 pKa = 4.57ASKK103 pKa = 10.86LSKK106 pKa = 10.07FLKK109 pKa = 10.13FSSIFLPKK117 pKa = 9.88GCYY120 pKa = 9.43YY121 pKa = 10.56LPDD124 pKa = 3.6MEE126 pKa = 4.9LVGFISSLASGASIFEE142 pKa = 4.26IKK144 pKa = 10.32LLPASGQMQGLLLIRR159 pKa = 11.84VSEE162 pKa = 4.36DD163 pKa = 3.19SLFKK167 pKa = 11.11YY168 pKa = 9.18EE169 pKa = 5.12QEE171 pKa = 4.29EE172 pKa = 4.01EE173 pKa = 4.26DD174 pKa = 3.53

Molecular weight: 19.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P9FIU5|A0A2P9FIU5_9VIRU Putative capsid protein OS=Poophage MBI-2016a OX=1926504 PE=4 SV=1
MM1 pKa = 7.46SSDD4 pKa = 3.19YY5 pKa = 10.63FQRR8 pKa = 11.84FEE10 pKa = 3.75LAYY13 pKa = 10.5APFFVRR19 pKa = 11.84KK20 pKa = 9.27RR21 pKa = 11.84VGKK24 pKa = 9.63RR25 pKa = 11.84FIVISRR31 pKa = 11.84FRR33 pKa = 11.84THH35 pKa = 6.53QRR37 pKa = 11.84ALDD40 pKa = 3.64YY41 pKa = 11.37LRR43 pKa = 11.84FLSVKK48 pKa = 10.37YY49 pKa = 9.94PGIYY53 pKa = 9.87FDD55 pKa = 4.2IKK57 pKa = 10.79DD58 pKa = 3.47VSFSYY63 pKa = 10.92LDD65 pKa = 3.59KK66 pKa = 11.07KK67 pKa = 11.27SSLL70 pKa = 3.71

Molecular weight: 8.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1828

58

647

261.1

29.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.783 ± 1.814

0.711 ± 0.37

6.182 ± 0.839

4.431 ± 0.766

6.182 ± 0.937

6.565 ± 0.561

1.204 ± 0.202

4.267 ± 0.503

4.759 ± 0.706

8.972 ± 0.984

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.243 ± 0.238

4.595 ± 0.702

4.759 ± 0.989

3.72 ± 0.509

6.346 ± 1.06

10.23 ± 0.984

3.884 ± 0.826

7.057 ± 0.713

1.532 ± 0.394

5.58 ± 0.63
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski