Cacao swollen shoot Ghana R virus
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H4U983|A0A2H4U983_9VIRU ORF2 protein OS=Cacao swollen shoot Ghana R virus OX=2056886 PE=4 SV=1
MM1 pKa = 7.1 FRR3 pKa = 11.84 LGEE6 pKa = 4.14 GMAGSSKK13 pKa = 8.2 QTIGEE18 pKa = 4.28 KK19 pKa = 10.21 FRR21 pKa = 11.84 SPGGNQFNLAKK32 pKa = 9.9 EE33 pKa = 4.17 YY34 pKa = 11.04 KK35 pKa = 9.91 DD36 pKa = 3.77 LCDD39 pKa = 3.63 DD40 pKa = 3.53 EE41 pKa = 5.95 RR42 pKa = 11.84 RR43 pKa = 11.84 ITAEE47 pKa = 4.62 GIPLPLLQAYY57 pKa = 6.95 QHH59 pKa = 5.93 EE60 pKa = 4.37 RR61 pKa = 11.84 ALCEE65 pKa = 4.0 EE66 pKa = 4.23 KK67 pKa = 11.09 AEE69 pKa = 4.07 NLLLEE74 pKa = 4.54 AMEE77 pKa = 3.83 EE78 pKa = 4.1 LIRR81 pKa = 11.84 IKK83 pKa = 9.74 EE84 pKa = 4.02 AKK86 pKa = 8.34 RR87 pKa = 11.84 AYY89 pKa = 9.36 VASHH93 pKa = 5.78 ATKK96 pKa = 10.57 DD97 pKa = 3.91 NYY99 pKa = 10.33 WGDD102 pKa = 3.93 CLPTVKK108 pKa = 10.48 DD109 pKa = 3.53 AEE111 pKa = 4.37 DD112 pKa = 3.5 RR113 pKa = 11.84 TKK115 pKa = 10.89 DD116 pKa = 3.14 VCNALRR122 pKa = 11.84 QVVEE126 pKa = 4.04 NSKK129 pKa = 10.9 DD130 pKa = 3.18 RR131 pKa = 11.84 YY132 pKa = 9.92
Molecular weight: 15.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.331
IPC2_protein 5.486
IPC_protein 5.372
Toseland 5.372
ProMoST 5.537
Dawson 5.397
Bjellqvist 5.499
Wikipedia 5.27
Rodwell 5.334
Grimsley 5.308
Solomon 5.397
Lehninger 5.359
Nozaki 5.537
DTASelect 5.652
Thurlkill 5.41
EMBOSS 5.347
Sillero 5.614
Patrickios 4.24
IPC_peptide 5.41
IPC2_peptide 5.614
IPC2.peptide.svr19 5.69
Protein with the highest isoelectric point:
>tr|A0A2H4U988|A0A2H4U988_9VIRU ORF1 protein OS=Cacao swollen shoot Ghana R virus OX=2056886 PE=4 SV=1
MM1 pKa = 7.22 GWLMSGNSYY10 pKa = 10.81 QEE12 pKa = 3.93 AFRR15 pKa = 11.84 NAAAYY20 pKa = 9.37 KK21 pKa = 8.49 PTGFLKK27 pKa = 10.7 SEE29 pKa = 3.99 EE30 pKa = 4.25 AGSGRR35 pKa = 11.84 EE36 pKa = 3.87 QLLSCISQHH45 pKa = 6.14 NLIIQLLVDD54 pKa = 3.95 LHH56 pKa = 7.13 DD57 pKa = 4.52 KK58 pKa = 10.79 VDD60 pKa = 3.71 NLSAEE65 pKa = 4.31 LKK67 pKa = 10.27 VLRR70 pKa = 11.84 KK71 pKa = 9.73 EE72 pKa = 3.84 KK73 pKa = 10.25 TKK75 pKa = 10.76 AQNPEE80 pKa = 4.23 EE81 pKa = 4.19 IEE83 pKa = 4.13 GLISQLKK90 pKa = 9.3 GLSIGPKK97 pKa = 9.41 EE98 pKa = 4.07 KK99 pKa = 10.92 VPEE102 pKa = 4.2 KK103 pKa = 10.58 KK104 pKa = 10.65 GNLYY108 pKa = 10.63 AFKK111 pKa = 10.35 DD112 pKa = 3.7 PYY114 pKa = 11.12 VILEE118 pKa = 4.03 EE119 pKa = 4.33 VKK121 pKa = 10.75 SKK123 pKa = 10.85 KK124 pKa = 10.31
Molecular weight: 13.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.781
IPC2_protein 7.819
IPC_protein 7.966
Toseland 8.609
ProMoST 8.639
Dawson 8.77
Bjellqvist 8.595
Wikipedia 8.814
Rodwell 8.916
Grimsley 8.448
Solomon 9.077
Lehninger 9.063
Nozaki 8.785
DTASelect 8.565
Thurlkill 8.712
EMBOSS 8.99
Sillero 8.873
Patrickios 4.902
IPC_peptide 9.063
IPC2_peptide 7.322
IPC2.peptide.svr19 7.427
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2227
124
1833
556.8
63.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.658 ± 0.905
1.706 ± 0.426
5.209 ± 0.812
8.487 ± 1.624
3.233 ± 0.486
5.972 ± 0.98
2.066 ± 0.179
7.185 ± 0.948
7.05 ± 1.766
7.634 ± 2.638
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.425 ± 0.481
4.535 ± 1.041
4.535 ± 0.9
6.286 ± 0.673
6.421 ± 0.98
6.691 ± 0.584
5.029 ± 1.004
4.894 ± 0.437
1.482 ± 0.371
3.502 ± 0.43
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here