Streptococcus phage Javan340
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6AZ73|A0A4D6AZ73_9CAUD HTH cro/C1-type domain-containing protein OS=Streptococcus phage Javan340 OX=2548114 GN=Javan340_0038 PE=4 SV=1
MM1 pKa = 7.52 FGMSYY6 pKa = 10.34 QEE8 pKa = 3.12 IHH10 pKa = 6.81 LFVEE14 pKa = 5.07 FLKK17 pKa = 10.63 EE18 pKa = 3.59 QYY20 pKa = 10.55 GQGRR24 pKa = 11.84 PDD26 pKa = 3.8 YY27 pKa = 11.01 IEE29 pKa = 5.37 ALDD32 pKa = 5.77 DD33 pKa = 4.31 LDD35 pKa = 5.07 CLVKK39 pKa = 10.48 VSYY42 pKa = 10.8 RR43 pKa = 11.84 EE44 pKa = 4.02 AIEE47 pKa = 4.99 RR48 pKa = 11.84 FLEE51 pKa = 4.53 DD52 pKa = 3.25 EE53 pKa = 4.4 VV54 pKa = 3.76
Molecular weight: 6.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.179
IPC2_protein 4.291
IPC_protein 4.139
Toseland 3.973
ProMoST 4.266
Dawson 4.088
Bjellqvist 4.253
Wikipedia 3.973
Rodwell 3.973
Grimsley 3.897
Solomon 4.075
Lehninger 4.037
Nozaki 4.215
DTASelect 4.342
Thurlkill 3.999
EMBOSS 3.999
Sillero 4.253
Patrickios 3.083
IPC_peptide 4.088
IPC2_peptide 4.24
IPC2.peptide.svr19 4.161
Protein with the highest isoelectric point:
>tr|A0A4D6B3Y2|A0A4D6B3Y2_9CAUD Uncharacterized protein OS=Streptococcus phage Javan340 OX=2548114 GN=Javan340_0027 PE=4 SV=1
MM1 pKa = 7.02 YY2 pKa = 9.35 NKK4 pKa = 9.94 PVRR7 pKa = 11.84 QSLKK11 pKa = 7.46 TRR13 pKa = 11.84 KK14 pKa = 8.55 WYY16 pKa = 10.4 KK17 pKa = 10.33 FRR19 pKa = 11.84 DD20 pKa = 3.4 KK21 pKa = 11.28 VMRR24 pKa = 11.84 QHH26 pKa = 7.64 DD27 pKa = 4.43 YY28 pKa = 11.25 LCQEE32 pKa = 4.3 SLRR35 pKa = 11.84 YY36 pKa = 8.47 GQSVPAEE43 pKa = 4.42 MVHH46 pKa = 6.75 HH47 pKa = 7.39 IYY49 pKa = 10.05 PVSEE53 pKa = 4.23 YY54 pKa = 10.47 PEE56 pKa = 4.33 LEE58 pKa = 4.17 YY59 pKa = 11.24 VSWNCLPLTNRR70 pKa = 11.84 KK71 pKa = 9.47 HH72 pKa = 5.18 NTFHH76 pKa = 7.57 DD77 pKa = 3.98 RR78 pKa = 11.84 NNDD81 pKa = 3.69 KK82 pKa = 10.87 IIGNGIYY89 pKa = 8.6 WQKK92 pKa = 10.66 KK93 pKa = 6.74 RR94 pKa = 11.84 KK95 pKa = 9.71 KK96 pKa = 9.88 EE97 pKa = 3.65 FLNFFKK103 pKa = 11.09 NKK105 pKa = 9.53 NEE107 pKa = 4.03 KK108 pKa = 10.16
Molecular weight: 13.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.121
IPC2_protein 9.268
IPC_protein 9.209
Toseland 9.867
ProMoST 9.633
Dawson 10.101
Bjellqvist 9.78
Wikipedia 10.262
Rodwell 10.526
Grimsley 10.175
Solomon 10.116
Lehninger 10.087
Nozaki 9.882
DTASelect 9.765
Thurlkill 9.94
EMBOSS 10.277
Sillero 10.014
Patrickios 9.853
IPC_peptide 10.116
IPC2_peptide 8.404
IPC2.peptide.svr19 8.182
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
43
0
43
8673
40
1347
201.7
22.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.48 ± 0.588
0.657 ± 0.156
5.996 ± 0.365
8.728 ± 0.508
4.497 ± 0.342
6.226 ± 0.405
1.533 ± 0.209
7.137 ± 0.305
8.209 ± 0.491
8.74 ± 0.502
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.433 ± 0.227
5.408 ± 0.37
2.675 ± 0.216
4.059 ± 0.315
4.6 ± 0.407
5.938 ± 0.43
6.019 ± 0.432
5.961 ± 0.324
1.28 ± 0.15
3.424 ± 0.345
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here