Amino acid dipepetide frequency for Escherichia phage vB_EcoM_WFbE185

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.87AlaAla: 4.87 ± 0.37
0.446AlaCys: 0.446 ± 0.087
3.922AlaAsp: 3.922 ± 0.265
5.131AlaGlu: 5.131 ± 0.317
2.212AlaPhe: 2.212 ± 0.193
4.127AlaGly: 4.127 ± 0.328
1.208AlaHis: 1.208 ± 0.146
5.0AlaIle: 5.0 ± 0.321
5.354AlaLys: 5.354 ± 0.341
5.893AlaLeu: 5.893 ± 0.327
1.413AlaMet: 1.413 ± 0.193
3.458AlaAsn: 3.458 ± 0.298
2.268AlaPro: 2.268 ± 0.204
2.417AlaGln: 2.417 ± 0.25
2.844AlaArg: 2.844 ± 0.226
4.926AlaSer: 4.926 ± 0.355
3.681AlaThr: 3.681 ± 0.471
4.22AlaVal: 4.22 ± 0.268
0.967AlaTrp: 0.967 ± 0.115
2.64AlaTyr: 2.64 ± 0.211
0.0AlaXaa: 0.0 ± 0.0
Cys
0.669CysAla: 0.669 ± 0.113
0.204CysCys: 0.204 ± 0.07
0.576CysAsp: 0.576 ± 0.095
0.781CysGlu: 0.781 ± 0.139
0.39CysPhe: 0.39 ± 0.089
0.706CysGly: 0.706 ± 0.12
0.297CysHis: 0.297 ± 0.08
0.651CysIle: 0.651 ± 0.111
0.744CysLys: 0.744 ± 0.121
0.595CysLeu: 0.595 ± 0.116
0.26CysMet: 0.26 ± 0.086
0.595CysAsn: 0.595 ± 0.1
0.613CysPro: 0.613 ± 0.122
0.353CysGln: 0.353 ± 0.073
0.688CysArg: 0.688 ± 0.126
0.837CysSer: 0.837 ± 0.13
0.558CysThr: 0.558 ± 0.092
0.595CysVal: 0.595 ± 0.1
0.149CysTrp: 0.149 ± 0.053
0.465CysTyr: 0.465 ± 0.1
0.0CysXaa: 0.0 ± 0.0
Asp
4.573AspAla: 4.573 ± 0.301
0.632AspCys: 0.632 ± 0.098
3.922AspAsp: 3.922 ± 0.304
4.629AspGlu: 4.629 ± 0.387
3.458AspPhe: 3.458 ± 0.256
5.186AspGly: 5.186 ± 0.326
0.892AspHis: 0.892 ± 0.125
4.908AspIle: 4.908 ± 0.325
4.015AspLys: 4.015 ± 0.32
4.833AspLeu: 4.833 ± 0.288
1.617AspMet: 1.617 ± 0.193
2.602AspAsn: 2.602 ± 0.191
2.082AspPro: 2.082 ± 0.25
1.524AspGln: 1.524 ± 0.145
2.231AspArg: 2.231 ± 0.214
3.755AspSer: 3.755 ± 0.245
3.253AspThr: 3.253 ± 0.282
4.629AspVal: 4.629 ± 0.305
1.19AspTrp: 1.19 ± 0.197
3.16AspTyr: 3.16 ± 0.249
0.0AspXaa: 0.0 ± 0.0
Glu
5.298GluAla: 5.298 ± 0.364
1.06GluCys: 1.06 ± 0.154
4.424GluAsp: 4.424 ± 0.305
4.963GluGlu: 4.963 ± 0.377
3.736GluPhe: 3.736 ± 0.277
3.736GluGly: 3.736 ± 0.277
1.134GluHis: 1.134 ± 0.158
6.06GluIle: 6.06 ± 0.408
4.815GluLys: 4.815 ± 0.381
6.469GluLeu: 6.469 ± 0.433
2.175GluMet: 2.175 ± 0.191
3.681GluAsn: 3.681 ± 0.239
1.859GluPro: 1.859 ± 0.175
2.547GluGln: 2.547 ± 0.216
2.974GluArg: 2.974 ± 0.204
4.127GluSer: 4.127 ± 0.346
4.313GluThr: 4.313 ± 0.286
5.0GluVal: 5.0 ± 0.358
0.818GluTrp: 0.818 ± 0.105
3.551GluTyr: 3.551 ± 0.29
0.0GluXaa: 0.0 ± 0.0
Phe
2.714PheAla: 2.714 ± 0.241
0.446PheCys: 0.446 ± 0.092
3.216PheAsp: 3.216 ± 0.238
3.718PheGlu: 3.718 ± 0.271
1.357PhePhe: 1.357 ± 0.149
2.677PheGly: 2.677 ± 0.201
0.706PheHis: 0.706 ± 0.129
3.067PheIle: 3.067 ± 0.215
4.127PheLys: 4.127 ± 0.333
2.454PheLeu: 2.454 ± 0.187
1.171PheMet: 1.171 ± 0.154
2.993PheAsn: 2.993 ± 0.222
1.06PhePro: 1.06 ± 0.143
1.301PheGln: 1.301 ± 0.2
1.859PheArg: 1.859 ± 0.22
2.881PheSer: 2.881 ± 0.221
2.64PheThr: 2.64 ± 0.232
2.918PheVal: 2.918 ± 0.259
0.576PheTrp: 0.576 ± 0.098
2.008PheTyr: 2.008 ± 0.192
0.0PheXaa: 0.0 ± 0.0
Gly
3.123GlyAla: 3.123 ± 0.31
0.576GlyCys: 0.576 ± 0.115
3.904GlyAsp: 3.904 ± 0.295
3.885GlyGlu: 3.885 ± 0.268
2.677GlyPhe: 2.677 ± 0.234
3.588GlyGly: 3.588 ± 0.284
1.041GlyHis: 1.041 ± 0.158
4.406GlyIle: 4.406 ± 0.35
4.406GlyLys: 4.406 ± 0.301
5.354GlyLeu: 5.354 ± 0.323
1.599GlyMet: 1.599 ± 0.194
3.272GlyAsn: 3.272 ± 0.378
1.822GlyPro: 1.822 ± 0.172
2.175GlyGln: 2.175 ± 0.228
2.881GlyArg: 2.881 ± 0.223
3.941GlySer: 3.941 ± 0.337
4.666GlyThr: 4.666 ± 0.465
3.922GlyVal: 3.922 ± 0.269
0.929GlyTrp: 0.929 ± 0.133
3.104GlyTyr: 3.104 ± 0.231
0.0GlyXaa: 0.0 ± 0.0
His
1.041HisAla: 1.041 ± 0.154
0.316HisCys: 0.316 ± 0.083
1.041HisAsp: 1.041 ± 0.135
1.115HisGlu: 1.115 ± 0.155
0.855HisPhe: 0.855 ± 0.13
1.041HisGly: 1.041 ± 0.161
0.372HisHis: 0.372 ± 0.08
1.376HisIle: 1.376 ± 0.176
1.19HisLys: 1.19 ± 0.145
1.245HisLeu: 1.245 ± 0.146
0.465HisMet: 0.465 ± 0.095
0.781HisAsn: 0.781 ± 0.141
0.874HisPro: 0.874 ± 0.142
0.595HisGln: 0.595 ± 0.115
0.874HisArg: 0.874 ± 0.149
1.115HisSer: 1.115 ± 0.194
0.911HisThr: 0.911 ± 0.177
1.041HisVal: 1.041 ± 0.122
0.26HisTrp: 0.26 ± 0.064
0.651HisTyr: 0.651 ± 0.11
0.0HisXaa: 0.0 ± 0.0
Ile
4.908IleAla: 4.908 ± 0.361
0.725IleCys: 0.725 ± 0.139
4.926IleAsp: 4.926 ± 0.37
5.261IleGlu: 5.261 ± 0.291
2.547IlePhe: 2.547 ± 0.209
4.294IleGly: 4.294 ± 0.307
1.06IleHis: 1.06 ± 0.144
4.777IleIle: 4.777 ± 0.323
6.952IleLys: 6.952 ± 0.447
4.257IleLeu: 4.257 ± 0.352
1.654IleMet: 1.654 ± 0.173
5.112IleAsn: 5.112 ± 0.326
2.658IlePro: 2.658 ± 0.201
2.602IleGln: 2.602 ± 0.22
3.29IleArg: 3.29 ± 0.302
4.592IleSer: 4.592 ± 0.321
4.796IleThr: 4.796 ± 0.341
4.461IleVal: 4.461 ± 0.308
0.558IleTrp: 0.558 ± 0.088
2.584IleTyr: 2.584 ± 0.219
0.0IleXaa: 0.0 ± 0.0
Lys
5.967LysAla: 5.967 ± 0.356
0.669LysCys: 0.669 ± 0.127
4.499LysAsp: 4.499 ± 0.309
5.893LysGlu: 5.893 ± 0.461
3.606LysPhe: 3.606 ± 0.244
4.666LysGly: 4.666 ± 0.252
1.561LysHis: 1.561 ± 0.173
5.186LysIle: 5.186 ± 0.355
4.777LysLys: 4.777 ± 0.338
6.357LysLeu: 6.357 ± 0.435
2.212LysMet: 2.212 ± 0.211
4.183LysAsn: 4.183 ± 0.328
2.435LysPro: 2.435 ± 0.219
2.268LysGln: 2.268 ± 0.226
3.142LysArg: 3.142 ± 0.266
4.313LysSer: 4.313 ± 0.275
4.499LysThr: 4.499 ± 0.3
4.889LysVal: 4.889 ± 0.342
1.283LysTrp: 1.283 ± 0.153
3.346LysTyr: 3.346 ± 0.218
0.0LysXaa: 0.0 ± 0.0
Leu
5.261LeuAla: 5.261 ± 0.338
0.911LeuCys: 0.911 ± 0.132
5.186LeuAsp: 5.186 ± 0.332
5.205LeuGlu: 5.205 ± 0.355
3.383LeuPhe: 3.383 ± 0.283
3.978LeuGly: 3.978 ± 0.281
1.097LeuHis: 1.097 ± 0.136
4.833LeuIle: 4.833 ± 0.284
5.744LeuLys: 5.744 ± 0.348
5.093LeuLeu: 5.093 ± 0.303
2.212LeuMet: 2.212 ± 0.194
4.889LeuAsn: 4.889 ± 0.301
3.104LeuPro: 3.104 ± 0.255
2.677LeuGln: 2.677 ± 0.231
3.588LeuArg: 3.588 ± 0.244
4.536LeuSer: 4.536 ± 0.289
4.573LeuThr: 4.573 ± 0.291
4.815LeuVal: 4.815 ± 0.34
0.688LeuTrp: 0.688 ± 0.126
3.179LeuTyr: 3.179 ± 0.228
0.0LeuXaa: 0.0 ± 0.0
Met
2.194MetAla: 2.194 ± 0.181
0.316MetCys: 0.316 ± 0.071
1.32MetAsp: 1.32 ± 0.15
1.84MetGlu: 1.84 ± 0.209
1.283MetPhe: 1.283 ± 0.178
1.301MetGly: 1.301 ± 0.176
0.297MetHis: 0.297 ± 0.074
1.692MetIle: 1.692 ± 0.178
2.417MetLys: 2.417 ± 0.252
2.212MetLeu: 2.212 ± 0.196
0.837MetMet: 0.837 ± 0.134
1.617MetAsn: 1.617 ± 0.173
0.781MetPro: 0.781 ± 0.128
0.706MetGln: 0.706 ± 0.115
1.06MetArg: 1.06 ± 0.143
1.989MetSer: 1.989 ± 0.18
1.692MetThr: 1.692 ± 0.173
1.431MetVal: 1.431 ± 0.162
0.223MetTrp: 0.223 ± 0.056
0.967MetTyr: 0.967 ± 0.132
0.0MetXaa: 0.0 ± 0.0
Asn
3.885AsnAla: 3.885 ± 0.298
0.409AsnCys: 0.409 ± 0.094
3.104AsnAsp: 3.104 ± 0.262
3.959AsnGlu: 3.959 ± 0.238
2.695AsnPhe: 2.695 ± 0.253
4.015AsnGly: 4.015 ± 0.389
1.041AsnHis: 1.041 ± 0.176
4.052AsnIle: 4.052 ± 0.247
4.071AsnLys: 4.071 ± 0.278
4.071AsnLeu: 4.071 ± 0.338
1.747AsnMet: 1.747 ± 0.152
3.458AsnAsn: 3.458 ± 0.285
2.584AsnPro: 2.584 ± 0.26
1.506AsnGln: 1.506 ± 0.184
2.138AsnArg: 2.138 ± 0.204
3.513AsnSer: 3.513 ± 0.26
2.993AsnThr: 2.993 ± 0.248
3.513AsnVal: 3.513 ± 0.274
0.576AsnTrp: 0.576 ± 0.103
2.063AsnTyr: 2.063 ± 0.164
0.0AsnXaa: 0.0 ± 0.0
Pro
2.063ProAla: 2.063 ± 0.214
0.39ProCys: 0.39 ± 0.082
2.231ProAsp: 2.231 ± 0.179
3.16ProGlu: 3.16 ± 0.289
1.636ProPhe: 1.636 ± 0.191
2.398ProGly: 2.398 ± 0.22
0.595ProHis: 0.595 ± 0.105
2.082ProIle: 2.082 ± 0.193
2.435ProLys: 2.435 ± 0.248
2.417ProLeu: 2.417 ± 0.241
0.725ProMet: 0.725 ± 0.126
2.045ProAsn: 2.045 ± 0.186
0.948ProPro: 0.948 ± 0.112
1.078ProGln: 1.078 ± 0.145
1.431ProArg: 1.431 ± 0.193
2.305ProSer: 2.305 ± 0.194
2.324ProThr: 2.324 ± 0.213
2.844ProVal: 2.844 ± 0.244
0.799ProTrp: 0.799 ± 0.125
1.357ProTyr: 1.357 ± 0.2
0.0ProXaa: 0.0 ± 0.0
Gln
2.417GlnAla: 2.417 ± 0.258
0.316GlnCys: 0.316 ± 0.079
1.673GlnAsp: 1.673 ± 0.215
2.379GlnGlu: 2.379 ± 0.232
1.561GlnPhe: 1.561 ± 0.192
1.915GlnGly: 1.915 ± 0.2
0.576GlnHis: 0.576 ± 0.12
2.881GlnIle: 2.881 ± 0.236
2.361GlnLys: 2.361 ± 0.193
2.658GlnLeu: 2.658 ± 0.245
0.985GlnMet: 0.985 ± 0.139
1.469GlnAsn: 1.469 ± 0.188
1.078GlnPro: 1.078 ± 0.123
1.134GlnGln: 1.134 ± 0.149
1.636GlnArg: 1.636 ± 0.157
1.357GlnSer: 1.357 ± 0.16
2.008GlnThr: 2.008 ± 0.224
2.454GlnVal: 2.454 ± 0.222
0.799GlnTrp: 0.799 ± 0.11
1.524GlnTyr: 1.524 ± 0.208
0.0GlnXaa: 0.0 ± 0.0
Arg
2.714ArgAla: 2.714 ± 0.248
0.52ArgCys: 0.52 ± 0.104
2.621ArgAsp: 2.621 ± 0.21
3.309ArgGlu: 3.309 ± 0.225
2.045ArgPhe: 2.045 ± 0.199
2.621ArgGly: 2.621 ± 0.249
0.651ArgHis: 0.651 ± 0.116
3.439ArgIle: 3.439 ± 0.25
3.588ArgLys: 3.588 ± 0.296
3.402ArgLeu: 3.402 ± 0.234
1.208ArgMet: 1.208 ± 0.167
2.045ArgAsn: 2.045 ± 0.162
1.357ArgPro: 1.357 ± 0.165
1.692ArgGln: 1.692 ± 0.201
2.045ArgArg: 2.045 ± 0.237
2.733ArgSer: 2.733 ± 0.196
2.361ArgThr: 2.361 ± 0.228
2.807ArgVal: 2.807 ± 0.243
0.502ArgTrp: 0.502 ± 0.128
1.673ArgTyr: 1.673 ± 0.21
0.0ArgXaa: 0.0 ± 0.0
Ser
3.402SerAla: 3.402 ± 0.28
0.632SerCys: 0.632 ± 0.109
3.941SerAsp: 3.941 ± 0.296
4.313SerGlu: 4.313 ± 0.316
2.807SerPhe: 2.807 ± 0.21
4.22SerGly: 4.22 ± 0.34
1.208SerHis: 1.208 ± 0.188
4.796SerIle: 4.796 ± 0.283
4.796SerLys: 4.796 ± 0.343
4.87SerLeu: 4.87 ± 0.277
1.524SerMet: 1.524 ± 0.155
2.993SerAsn: 2.993 ± 0.267
2.398SerPro: 2.398 ± 0.227
2.305SerGln: 2.305 ± 0.205
2.9SerArg: 2.9 ± 0.247
4.722SerSer: 4.722 ± 0.337
3.922SerThr: 3.922 ± 0.314
3.978SerVal: 3.978 ± 0.33
0.948SerTrp: 0.948 ± 0.152
2.807SerTyr: 2.807 ± 0.246
0.0SerXaa: 0.0 ± 0.0
Thr
3.959ThrAla: 3.959 ± 0.348
0.576ThrCys: 0.576 ± 0.125
4.09ThrAsp: 4.09 ± 0.33
4.108ThrGlu: 4.108 ± 0.237
2.435ThrPhe: 2.435 ± 0.237
4.183ThrGly: 4.183 ± 0.392
1.208ThrHis: 1.208 ± 0.158
4.201ThrIle: 4.201 ± 0.297
4.183ThrLys: 4.183 ± 0.292
4.257ThrLeu: 4.257 ± 0.337
1.171ThrMet: 1.171 ± 0.157
2.863ThrAsn: 2.863 ± 0.209
3.049ThrPro: 3.049 ± 0.304
1.859ThrGln: 1.859 ± 0.234
2.64ThrArg: 2.64 ± 0.282
3.476ThrSer: 3.476 ± 0.353
3.829ThrThr: 3.829 ± 0.354
4.722ThrVal: 4.722 ± 0.421
0.948ThrTrp: 0.948 ± 0.134
2.733ThrTyr: 2.733 ± 0.187
0.0ThrXaa: 0.0 ± 0.0
Val
4.183ValAla: 4.183 ± 0.295
0.892ValCys: 0.892 ± 0.128
4.703ValAsp: 4.703 ± 0.289
5.502ValGlu: 5.502 ± 0.358
2.64ValPhe: 2.64 ± 0.226
3.569ValGly: 3.569 ± 0.326
1.134ValHis: 1.134 ± 0.148
4.48ValIle: 4.48 ± 0.283
5.484ValLys: 5.484 ± 0.359
4.74ValLeu: 4.74 ± 0.298
1.58ValMet: 1.58 ± 0.196
3.755ValAsn: 3.755 ± 0.329
2.082ValPro: 2.082 ± 0.205
2.342ValGln: 2.342 ± 0.253
3.086ValArg: 3.086 ± 0.232
4.722ValSer: 4.722 ± 0.268
4.071ValThr: 4.071 ± 0.324
4.796ValVal: 4.796 ± 0.296
0.799ValTrp: 0.799 ± 0.107
2.714ValTyr: 2.714 ± 0.238
0.0ValXaa: 0.0 ± 0.0
Trp
0.892TrpAla: 0.892 ± 0.121
0.149TrpCys: 0.149 ± 0.056
0.855TrpAsp: 0.855 ± 0.129
0.781TrpGlu: 0.781 ± 0.112
0.725TrpPhe: 0.725 ± 0.113
0.539TrpGly: 0.539 ± 0.109
0.149TrpHis: 0.149 ± 0.054
1.078TrpIle: 1.078 ± 0.145
1.376TrpLys: 1.376 ± 0.164
0.874TrpLeu: 0.874 ± 0.121
0.446TrpMet: 0.446 ± 0.093
0.929TrpAsn: 0.929 ± 0.12
0.502TrpPro: 0.502 ± 0.092
0.52TrpGln: 0.52 ± 0.092
0.446TrpArg: 0.446 ± 0.094
0.874TrpSer: 0.874 ± 0.12
0.855TrpThr: 0.855 ± 0.107
0.967TrpVal: 0.967 ± 0.117
0.167TrpTrp: 0.167 ± 0.061
0.744TrpTyr: 0.744 ± 0.122
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.863TyrAla: 2.863 ± 0.219
0.539TyrCys: 0.539 ± 0.114
2.937TyrAsp: 2.937 ± 0.211
2.807TyrGlu: 2.807 ± 0.279
1.84TyrPhe: 1.84 ± 0.181
2.51TyrGly: 2.51 ± 0.203
0.929TyrHis: 0.929 ± 0.124
3.049TyrIle: 3.049 ± 0.244
2.937TyrLys: 2.937 ± 0.222
2.9TyrLeu: 2.9 ± 0.238
1.153TyrMet: 1.153 ± 0.156
2.658TyrAsn: 2.658 ± 0.225
1.71TyrPro: 1.71 ± 0.181
1.58TyrGln: 1.58 ± 0.171
1.58TyrArg: 1.58 ± 0.163
2.77TyrSer: 2.77 ± 0.208
2.547TyrThr: 2.547 ± 0.209
3.272TyrVal: 3.272 ± 0.253
0.688TyrTrp: 0.688 ± 0.122
1.692TyrTyr: 1.692 ± 0.196
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 277 proteins (53796 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski