Flavobacteriales bacterium ALC-1

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; unclassified Flavobacteriales

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3427 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8UHX2|A8UHX2_9FLAO Putative transporter permease protein OS=Flavobacteriales bacterium ALC-1 OX=391603 GN=FBALC1_04537 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 10.39RR3 pKa = 11.84IVYY6 pKa = 10.23LLLLLPFTISAQFSVSSTTPASNEE30 pKa = 3.58LDD32 pKa = 3.47VSVTSDD38 pKa = 2.47ISINFNQNVDD48 pKa = 3.34AATLTNANISITGNNGIVLGFSAPIVTGTNAIFNPDD84 pKa = 3.03RR85 pKa = 11.84DD86 pKa = 4.11FFPGEE91 pKa = 4.39IITITITTDD100 pKa = 3.02VEE102 pKa = 4.32NSSGTNLNKK111 pKa = 10.44AFTFQFTAEE120 pKa = 4.1VCLNSPGQFPLGEE133 pKa = 4.2NTISLATDD141 pKa = 3.93GAISVTIADD150 pKa = 4.84LDD152 pKa = 4.19DD153 pKa = 6.36DD154 pKa = 5.09GDD156 pKa = 4.55LDD158 pKa = 3.83ILSASSLDD166 pKa = 5.78DD167 pKa = 4.5RR168 pKa = 11.84IAWYY172 pKa = 10.68ANDD175 pKa = 3.79GTGKK179 pKa = 10.5FGVQQTITTSADD191 pKa = 3.07YY192 pKa = 10.97AHH194 pKa = 7.52RR195 pKa = 11.84VTTADD200 pKa = 3.93LDD202 pKa = 4.02GDD204 pKa = 3.95GDD206 pKa = 3.86IDD208 pKa = 4.28VISASSVDD216 pKa = 5.72DD217 pKa = 4.58RR218 pKa = 11.84IAWYY222 pKa = 10.31EE223 pKa = 3.61NDD225 pKa = 3.27GLGNFGPQQTITTAANLAYY244 pKa = 10.49NVIASDD250 pKa = 4.45LDD252 pKa = 3.28QDD254 pKa = 3.42GDD256 pKa = 3.63MDD258 pKa = 4.78VISASAFDD266 pKa = 5.68GLIAWYY272 pKa = 10.15EE273 pKa = 3.76NDD275 pKa = 3.21GLGNFGALQTITTAAPGARR294 pKa = 11.84SVYY297 pKa = 10.3IADD300 pKa = 4.69LDD302 pKa = 3.85NDD304 pKa = 3.66GDD306 pKa = 4.07IDD308 pKa = 4.13VLSASSDD315 pKa = 3.56DD316 pKa = 5.91DD317 pKa = 3.51RR318 pKa = 11.84VAWYY322 pKa = 10.17EE323 pKa = 3.71NDD325 pKa = 3.16GSGNFGAQQTITTLADD341 pKa = 3.21RR342 pKa = 11.84AFSVKK347 pKa = 10.41AADD350 pKa = 4.12LDD352 pKa = 4.11GDD354 pKa = 3.7GDD356 pKa = 3.9MDD358 pKa = 4.81VIAACSSTSTARR370 pKa = 11.84DD371 pKa = 3.23WRR373 pKa = 11.84ITWYY377 pKa = 10.23EE378 pKa = 3.82NLGSGTFGPQQTISINVFQARR399 pKa = 11.84SVYY402 pKa = 9.77TADD405 pKa = 4.59LDD407 pKa = 4.21GDD409 pKa = 3.87GDD411 pKa = 4.97LDD413 pKa = 3.86VLSASSRR420 pKa = 11.84DD421 pKa = 4.32DD422 pKa = 4.02RR423 pKa = 11.84IAWYY427 pKa = 9.79EE428 pKa = 3.75NDD430 pKa = 3.31GSGSFGAQQTVTTNADD446 pKa = 3.7GAWSIVAADD455 pKa = 4.4LDD457 pKa = 4.31GDD459 pKa = 3.94GDD461 pKa = 3.83MDD463 pKa = 6.35LISGDD468 pKa = 3.75YY469 pKa = 11.02NGDD472 pKa = 3.09RR473 pKa = 11.84VAWYY477 pKa = 9.54EE478 pKa = 4.0NAVHH482 pKa = 7.77DD483 pKa = 4.98DD484 pKa = 3.55RR485 pKa = 11.84TLATLGGTDD494 pKa = 4.56LVLSPPFDD502 pKa = 4.92PNVSSYY508 pKa = 9.53TAAVCNSVLSTTINATVTNSGDD530 pKa = 3.87TIGGTGLVNLNEE542 pKa = 4.5GTNMITVSVTDD553 pKa = 3.39QCGGTDD559 pKa = 3.65DD560 pKa = 3.94YY561 pKa = 10.87TVEE564 pKa = 4.16VTRR567 pKa = 11.84VPLITQQITWSGSVSTDD584 pKa = 2.42WNNPNNWTPNIVPLEE599 pKa = 4.1CLEE602 pKa = 4.37IIIPEE607 pKa = 4.32TTNQPLISPGVINIKK622 pKa = 10.2DD623 pKa = 3.66LNINSGSNLEE633 pKa = 4.25IPVGATLNVSDD644 pKa = 5.05DD645 pKa = 3.71LNMYY649 pKa = 10.15SEE651 pKa = 4.78SDD653 pKa = 3.64TYY655 pKa = 11.52SGIIVKK661 pKa = 10.24GVLSVSGKK669 pKa = 8.02TKK671 pKa = 7.73YY672 pKa = 9.97HH673 pKa = 7.36RR674 pKa = 11.84YY675 pKa = 8.04TNSQANGNDD684 pKa = 4.31LIAPPLSGQSWTSFLTNDD702 pKa = 3.27ANHH705 pKa = 6.52NEE707 pKa = 3.61GLIYY711 pKa = 10.84NNGANPVTTYY721 pKa = 11.29LFGPFEE727 pKa = 4.72KK728 pKa = 10.74GATDD732 pKa = 5.79DD733 pKa = 4.27YY734 pKa = 11.6ILYY737 pKa = 10.02NDD739 pKa = 4.09NSSEE743 pKa = 3.9FLISGKK749 pKa = 9.67GYY751 pKa = 10.22RR752 pKa = 11.84SATNTVNGDD761 pKa = 3.45ALIFTGSIVTGAVNTTIINEE781 pKa = 4.36TTGGFPEE788 pKa = 4.43WNLIGNPYY796 pKa = 8.23PTYY799 pKa = 10.44IDD801 pKa = 3.46VNTFLNHH808 pKa = 5.82VGSVSGVTNLSLLSDD823 pKa = 3.84STAAVYY829 pKa = 9.92GYY831 pKa = 10.69DD832 pKa = 4.41GDD834 pKa = 4.14DD835 pKa = 3.57TDD837 pKa = 4.16VSGSVWTYY845 pKa = 8.32TNLVEE850 pKa = 4.82GPTLIAPGQGFFVSSKK866 pKa = 9.05YY867 pKa = 10.58ASSNIEE873 pKa = 4.05FTPDD877 pKa = 3.19MQIKK881 pKa = 10.24GSSDD885 pKa = 3.68DD886 pKa = 4.18FIQGRR891 pKa = 11.84TINNTDD897 pKa = 3.87YY898 pKa = 11.17IKK900 pKa = 11.21LNAATSSKK908 pKa = 10.71SYY910 pKa = 8.74STSFYY915 pKa = 10.18FHH917 pKa = 7.08NNGSQGLDD925 pKa = 2.99KK926 pKa = 11.17GYY928 pKa = 10.66DD929 pKa = 3.16AAVFGGALPSFSLYY943 pKa = 10.49SHH945 pKa = 6.99LVAEE949 pKa = 4.6NEE951 pKa = 4.42GIPMVIQTLSSDD963 pKa = 4.0DD964 pKa = 3.57IGNVIVPLGLNVNNGEE980 pKa = 4.1QVTISLGNYY989 pKa = 7.69NIPDD993 pKa = 3.59NVSVYY998 pKa = 11.14LEE1000 pKa = 4.65DD1001 pKa = 3.72NVNNTFTRR1009 pKa = 11.84LDD1011 pKa = 3.39TTDD1014 pKa = 3.95YY1015 pKa = 11.26IFTTVTALNGTGRR1028 pKa = 11.84FYY1030 pKa = 11.54LHH1032 pKa = 6.64FTTEE1036 pKa = 4.36SLLSTGEE1043 pKa = 4.15HH1044 pKa = 5.93NFKK1047 pKa = 10.62EE1048 pKa = 4.18FNIYY1052 pKa = 9.44TNQLGRR1058 pKa = 11.84TIIIEE1063 pKa = 4.17GNLTNKK1069 pKa = 9.6VDD1071 pKa = 3.72VVVFDD1076 pKa = 3.35ILGRR1080 pKa = 11.84EE1081 pKa = 4.6VLRR1084 pKa = 11.84QDD1086 pKa = 5.03LNHH1089 pKa = 6.78LVNKK1093 pKa = 10.36NIINANTLGSGVYY1106 pKa = 10.36VITIEE1111 pKa = 4.75DD1112 pKa = 3.67GNRR1115 pKa = 11.84QYY1117 pKa = 11.0SQKK1120 pKa = 10.85LILKK1124 pKa = 9.44

Molecular weight: 120.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8UH16|A8UH16_9FLAO Transcriptional repressor of sporulation and degradative enzyme production OS=Flavobacteriales bacterium ALC-1 OX=391603 GN=FBALC1_13512 PE=4 SV=1
MM1 pKa = 7.3ARR3 pKa = 11.84IAGVDD8 pKa = 2.98IPKK11 pKa = 10.23NKK13 pKa = 9.96RR14 pKa = 11.84GVISLTYY21 pKa = 8.93IYY23 pKa = 10.94GVGRR27 pKa = 11.84SRR29 pKa = 11.84AKK31 pKa = 10.5EE32 pKa = 3.56ILATAKK38 pKa = 10.09VDD40 pKa = 3.51EE41 pKa = 4.52STKK44 pKa = 9.93VQDD47 pKa = 3.51WSDD50 pKa = 3.97DD51 pKa = 3.89EE52 pKa = 4.88IGAIRR57 pKa = 11.84EE58 pKa = 4.08AVGTFKK64 pKa = 10.66IEE66 pKa = 3.87GEE68 pKa = 4.25LRR70 pKa = 11.84SEE72 pKa = 3.86TQMNIKK78 pKa = 10.21RR79 pKa = 11.84LMDD82 pKa = 3.76IGCYY86 pKa = 9.34RR87 pKa = 11.84GIRR90 pKa = 11.84HH91 pKa = 6.13RR92 pKa = 11.84VGLPLRR98 pKa = 11.84GQRR101 pKa = 11.84TKK103 pKa = 11.3NNSRR107 pKa = 11.84TRR109 pKa = 11.84KK110 pKa = 9.17GRR112 pKa = 11.84RR113 pKa = 11.84KK114 pKa = 7.81TVANKK119 pKa = 10.49KK120 pKa = 10.06KK121 pKa = 8.78VTKK124 pKa = 10.51

Molecular weight: 14.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3427

0

3427

1172252

17

3247

342.1

38.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.138 ± 0.036

0.745 ± 0.017

5.977 ± 0.039

6.374 ± 0.038

5.215 ± 0.036

6.266 ± 0.053

1.694 ± 0.019

8.239 ± 0.043

7.817 ± 0.067

9.179 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.093 ± 0.02

6.612 ± 0.047

3.216 ± 0.027

3.185 ± 0.022

3.248 ± 0.029

6.748 ± 0.034

5.963 ± 0.059

6.1 ± 0.028

1.033 ± 0.016

4.16 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski