Fomitiporia mediterranea (strain MF3/22) (Grapevine white-rot fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Hymenochaetales; Hymenochaetaceae; Fomitiporia; Fomitiporia mediterranea

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 531 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7SG48|R7SG48_FOMME Uncharacterized protein (Fragment) OS=Fomitiporia mediterranea (strain MF3/22) OX=694068 GN=FOMMEDRAFT_24479 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84FLNTQIVVFACVLSAHH18 pKa = 6.48ALISRR23 pKa = 11.84AVPKK27 pKa = 10.51SVIDD31 pKa = 3.77SCPSAQEE38 pKa = 4.0EE39 pKa = 4.93SSTSILINGEE49 pKa = 4.12EE50 pKa = 4.53IIHH53 pKa = 6.32QSFTCPDD60 pKa = 3.3GKK62 pKa = 10.8FDD64 pKa = 6.19DD65 pKa = 5.55DD66 pKa = 3.56PTSRR70 pKa = 11.84RR71 pKa = 11.84DD72 pKa = 3.44YY73 pKa = 10.7NAGGGSLWKK82 pKa = 10.24RR83 pKa = 11.84SSLEE87 pKa = 3.63ARR89 pKa = 11.84DD90 pKa = 4.18TIDD93 pKa = 4.79CEE95 pKa = 4.82FPQLMPEE102 pKa = 4.33CQCGRR107 pKa = 11.84EE108 pKa = 3.97FSCICFEE115 pKa = 4.42GDD117 pKa = 3.46VAPEE121 pKa = 4.13EE122 pKa = 4.31FDD124 pKa = 3.61CTQLLNMVTSANEE137 pKa = 3.77DD138 pKa = 3.38AQTFTVAANGADD150 pKa = 3.89VISLRR155 pKa = 11.84TCALIFVNNQDD166 pKa = 3.83IAVDD170 pKa = 3.9YY171 pKa = 10.92CWDD174 pKa = 3.67NLVSQIVLSVQNLDD188 pKa = 3.3

Molecular weight: 20.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7SG29|R7SG29_FOMME Transcription initiation factor IIE subunit alpha OS=Fomitiporia mediterranea (strain MF3/22) OX=694068 GN=FOMMEDRAFT_115230 PE=3 SV=1
MM1 pKa = 7.74ASLAAQISLFNAPDD15 pKa = 4.19PNLGAPLTLAARR27 pKa = 11.84CHH29 pKa = 5.28GTASASTTCSTSIHH43 pKa = 5.47TRR45 pKa = 11.84MQFNLQGPSDD55 pKa = 4.08DD56 pKa = 3.65QSVVMKK62 pKa = 7.35TTSNGPRR69 pKa = 11.84THH71 pKa = 7.2PSSSVADD78 pKa = 3.56AVGKK82 pKa = 8.37RR83 pKa = 11.84TRR85 pKa = 11.84QRR87 pKa = 11.84QLIIHH92 pKa = 6.15YY93 pKa = 9.4KK94 pKa = 9.31YY95 pKa = 11.24SNLTRR100 pKa = 11.84SPPIVHH106 pKa = 6.51CMAARR111 pKa = 11.84SDD113 pKa = 3.01IRR115 pKa = 11.84TRR117 pKa = 11.84RR118 pKa = 11.84AVACAKK124 pKa = 9.48WRR126 pKa = 11.84WQTLVILRR134 pKa = 11.84WVMRR138 pKa = 11.84CACKK142 pKa = 10.58DD143 pKa = 3.16GSTTDD148 pKa = 3.33AVITVEE154 pKa = 4.45PLNALAFGWSVWIWSRR170 pKa = 11.84DD171 pKa = 3.5GPGNRR176 pKa = 11.84HH177 pKa = 5.58PP178 pKa = 4.61

Molecular weight: 19.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

531

0

531

161738

49

2033

304.6

33.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.012 ± 0.114

1.535 ± 0.054

5.636 ± 0.079

6.441 ± 0.138

3.732 ± 0.08

5.9 ± 0.101

2.836 ± 0.077

5.02 ± 0.102

4.73 ± 0.123

9.648 ± 0.149

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.003 ± 0.045

3.954 ± 0.062

5.448 ± 0.12

3.778 ± 0.074

6.459 ± 0.108

8.638 ± 0.141

6.046 ± 0.086

6.112 ± 0.107

1.357 ± 0.043

2.716 ± 0.052
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski