Novosphingobium sp. Fuku2-ISO-50

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium; unclassified Novosphingobium

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3834 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A117V1C9|A0A117V1C9_9SPHN Uncharacterized protein OS=Novosphingobium sp. Fuku2-ISO-50 OX=1739114 GN=AQZ50_16665 PE=3 SV=1
MM1 pKa = 7.7SYY3 pKa = 10.78AISLSGLQNAQTDD16 pKa = 3.84LSVIGNNIANANTTGFKK33 pKa = 10.24EE34 pKa = 4.67SNVQFANLVAASAYY48 pKa = 10.67SNPKK52 pKa = 9.53DD53 pKa = 3.31IQGIGSTVSAIDD65 pKa = 3.36QNFSQGPINQTGSALDD81 pKa = 3.7LAITGDD87 pKa = 3.72GFFTVTSPITTQTYY101 pKa = 6.14YY102 pKa = 10.46TRR104 pKa = 11.84NGNFSLDD111 pKa = 3.41STTAATSGNSYY122 pKa = 10.51IVDD125 pKa = 3.73SNGNRR130 pKa = 11.84LQALPTSATGTVTSTTPTDD149 pKa = 3.41ATVPATDD156 pKa = 3.47AAGSAFSGVTIAANGNISASYY177 pKa = 10.84ADD179 pKa = 3.91GSNTVIGKK187 pKa = 9.08VALAAFAAPEE197 pKa = 4.11GLKK200 pKa = 10.28QVGSADD206 pKa = 3.53YY207 pKa = 8.63TATGLSGTATFGAPNSGQYY226 pKa = 11.09GSLLSGSLEE235 pKa = 3.87ASNVDD240 pKa = 3.84LSSEE244 pKa = 4.15MVNLITAQQYY254 pKa = 8.12FQANAKK260 pKa = 10.49AIDD263 pKa = 3.75TNTTVSEE270 pKa = 4.52AIINLHH276 pKa = 5.47SS277 pKa = 3.61

Molecular weight: 27.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A102DF85|A0A102DF85_9SPHN Dihydroorotase OS=Novosphingobium sp. Fuku2-ISO-50 OX=1739114 GN=pyrC PE=3 SV=1
MM1 pKa = 7.81RR2 pKa = 11.84IEE4 pKa = 4.08RR5 pKa = 11.84MNGRR9 pKa = 11.84GLRR12 pKa = 11.84MTSATPLRR20 pKa = 11.84VVAASALLLAAGGCSTIRR38 pKa = 11.84DD39 pKa = 3.58HH40 pKa = 7.74RR41 pKa = 11.84GYY43 pKa = 10.89LVDD46 pKa = 3.93PALTQSVLPGVDD58 pKa = 2.85NRR60 pKa = 11.84LSVEE64 pKa = 4.01KK65 pKa = 10.92SLGQPTLKK73 pKa = 10.66SQFGQQSWYY82 pKa = 10.0YY83 pKa = 10.19VSLDD87 pKa = 3.29TVQRR91 pKa = 11.84PFTRR95 pKa = 11.84PRR97 pKa = 11.84IHH99 pKa = 6.83AGSILRR105 pKa = 11.84VSFDD109 pKa = 3.15TAGTVQSVSNDD120 pKa = 3.31GVEE123 pKa = 3.88HH124 pKa = 6.6AQRR127 pKa = 11.84IVPDD131 pKa = 3.38RR132 pKa = 11.84HH133 pKa = 5.21KK134 pKa = 9.82TPTLGRR140 pKa = 11.84DD141 pKa = 2.74RR142 pKa = 11.84SFFEE146 pKa = 5.36DD147 pKa = 3.31LFGNIGTVGAMPGAQGGQPGGGGGGRR173 pKa = 11.84GGGGTGPNGSS183 pKa = 3.72

Molecular weight: 19.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3834

0

3834

1291571

29

2696

336.9

36.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.93 ± 0.063

0.853 ± 0.013

5.942 ± 0.029

4.866 ± 0.033

3.528 ± 0.022

9.091 ± 0.042

2.315 ± 0.018

4.975 ± 0.027

2.574 ± 0.027

9.911 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.407 ± 0.018

2.499 ± 0.029

5.474 ± 0.032

3.07 ± 0.023

7.174 ± 0.041

5.0 ± 0.033

5.401 ± 0.04

7.273 ± 0.034

1.481 ± 0.016

2.237 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski