Pacific flying fox faeces associated gemycircularvirus-4

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; Cyclovirus; unclassified Cyclovirus

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140CTM2|A0A140CTM2_9CIRC RepA OS=Pacific flying fox faeces associated gemycircularvirus-4 OX=1795996 PE=3 SV=1
MM1 pKa = 7.47TFLFSARR8 pKa = 11.84YY9 pKa = 9.34VLLTYY14 pKa = 7.24PQCGTLDD21 pKa = 3.23GWAVSDD27 pKa = 4.02HH28 pKa = 6.52LSALGAEE35 pKa = 4.64CIVGRR40 pKa = 11.84EE41 pKa = 4.0NHH43 pKa = 6.3SDD45 pKa = 3.41GGTHH49 pKa = 6.32LHH51 pKa = 6.56AFVDD55 pKa = 4.89FGRR58 pKa = 11.84KK59 pKa = 7.96KK60 pKa = 9.87QSRR63 pKa = 11.84RR64 pKa = 11.84SDD66 pKa = 3.46FFDD69 pKa = 3.73VGGHH73 pKa = 6.35HH74 pKa = 7.14PNIAPSRR81 pKa = 11.84GRR83 pKa = 11.84PEE85 pKa = 3.88RR86 pKa = 11.84GYY88 pKa = 11.11DD89 pKa = 3.48YY90 pKa = 10.71AIKK93 pKa = 10.87DD94 pKa = 3.59GDD96 pKa = 4.02VVAGGLARR104 pKa = 11.84PGGGGLPEE112 pKa = 4.55IANKK116 pKa = 9.29WSEE119 pKa = 4.1IVSAEE124 pKa = 3.84SRR126 pKa = 11.84EE127 pKa = 4.02QFFDD131 pKa = 4.51LLRR134 pKa = 11.84QLDD137 pKa = 4.12PKK139 pKa = 10.46TLVTRR144 pKa = 11.84WTEE147 pKa = 3.7LNKK150 pKa = 10.8YY151 pKa = 10.14ADD153 pKa = 3.7AAYY156 pKa = 10.25APTPEE161 pKa = 5.03PYY163 pKa = 10.25VGSDD167 pKa = 2.91GKK169 pKa = 10.54QFEE172 pKa = 4.79LGMVPEE178 pKa = 4.41LARR181 pKa = 11.84WGEE184 pKa = 3.83QLVANDD190 pKa = 4.12PVEE193 pKa = 4.89GKK195 pKa = 10.38CSPLPGPPRR204 pKa = 11.84GGHH207 pKa = 5.86GGFPGGGPLPLPLALRR223 pKa = 11.84DD224 pKa = 3.5SRR226 pKa = 11.84RR227 pKa = 11.84AMLIVV232 pKa = 3.38

Molecular weight: 25.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140CTM1|A0A140CTM1_9CIRC Replication-associated protein OS=Pacific flying fox faeces associated gemycircularvirus-4 OX=1795996 PE=3 SV=1
MM1 pKa = 7.54PLKK4 pKa = 10.21RR5 pKa = 11.84YY6 pKa = 9.5KK7 pKa = 10.66GRR9 pKa = 11.84NSVSYY14 pKa = 10.39SRR16 pKa = 11.84RR17 pKa = 11.84SKK19 pKa = 10.55SRR21 pKa = 11.84KK22 pKa = 8.63PSPKK26 pKa = 8.98KK27 pKa = 10.03KK28 pKa = 10.27SYY30 pKa = 11.04AKK32 pKa = 10.06RR33 pKa = 11.84KK34 pKa = 7.3TMTRR38 pKa = 11.84KK39 pKa = 9.49RR40 pKa = 11.84VVNIASTKK48 pKa = 10.81KK49 pKa = 8.32MDD51 pKa = 3.44TMSTVSIRR59 pKa = 11.84PDD61 pKa = 2.89GSAFRR66 pKa = 11.84EE67 pKa = 4.11PFVMNGDD74 pKa = 3.53TTYY77 pKa = 10.44IIPWVATWRR86 pKa = 11.84NFQTSSTNTVKK97 pKa = 10.64GLKK100 pKa = 9.84ADD102 pKa = 3.88PTRR105 pKa = 11.84SATSCYY111 pKa = 9.37MRR113 pKa = 11.84GLKK116 pKa = 9.62EE117 pKa = 4.03QIQLATNDD125 pKa = 3.86GQCWQWRR132 pKa = 11.84RR133 pKa = 11.84ICFTMKK139 pKa = 10.71GDD141 pKa = 5.11DD142 pKa = 3.39IYY144 pKa = 11.68AAAGPSFRR152 pKa = 11.84VAFQNSGGYY161 pKa = 8.76QRR163 pKa = 11.84LFSDD167 pKa = 3.95WNNTKK172 pKa = 10.63GGTTPPINFLVDD184 pKa = 4.3PIFKK188 pKa = 8.96GTAGTDD194 pKa = 2.77WDD196 pKa = 4.68DD197 pKa = 4.15PFVAPLDD204 pKa = 3.89PYY206 pKa = 10.85RR207 pKa = 11.84IGVKK211 pKa = 9.67YY212 pKa = 10.89DD213 pKa = 2.84KK214 pKa = 10.43TRR216 pKa = 11.84YY217 pKa = 8.9IRR219 pKa = 11.84SGNNFGMLKK228 pKa = 9.2MVKK231 pKa = 9.52LWHH234 pKa = 6.71PMNKK238 pKa = 8.34TLIYY242 pKa = 10.77NDD244 pKa = 4.48DD245 pKa = 3.89EE246 pKa = 5.3NGGVTDD252 pKa = 4.56AAATSVRR259 pKa = 11.84THH261 pKa = 5.79GMGDD265 pKa = 3.71YY266 pKa = 10.82YY267 pKa = 11.11VIDD270 pKa = 3.93MFRR273 pKa = 11.84AATGAVAGSQLNIRR287 pKa = 11.84SSGG290 pKa = 3.33

Molecular weight: 32.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

858

232

336

286.0

31.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.809 ± 0.348

1.166 ± 0.05

6.41 ± 0.078

3.963 ± 1.084

4.429 ± 0.162

10.606 ± 0.956

1.981 ± 0.476

3.263 ± 0.595

5.128 ± 1.033

7.809 ± 1.496

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.214 ± 0.723

3.613 ± 0.7

6.41 ± 0.684

2.681 ± 0.032

7.459 ± 0.189

6.41 ± 0.566

5.828 ± 1.152

6.177 ± 0.333

2.681 ± 0.446

3.963 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski