Lactococcus phage GE1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Chertseyvirus; Lactococcus virus GE1

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N9BAV4|A0A0N9BAV4_9CAUD Uncharacterized protein OS=Lactococcus phage GE1 OX=1698369 PE=4 SV=1
MM1 pKa = 7.54TSPIKK6 pKa = 9.82LTPQEE11 pKa = 3.91MDD13 pKa = 3.45MLLKK17 pKa = 10.74DD18 pKa = 2.96IATQIEE24 pKa = 4.75YY25 pKa = 11.02VEE27 pKa = 5.61DD28 pKa = 3.88NLDD31 pKa = 4.16DD32 pKa = 4.16YY33 pKa = 11.37PVYY36 pKa = 10.4NTDD39 pKa = 3.15IQNLSDD45 pKa = 5.22PIRR48 pKa = 11.84EE49 pKa = 3.96ILEE52 pKa = 4.03KK53 pKa = 10.44GGYY56 pKa = 6.94MM57 pKa = 4.6

Molecular weight: 6.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N9BBD8|A0A0N9BBD8_9CAUD Uncharacterized protein OS=Lactococcus phage GE1 OX=1698369 PE=4 SV=1
MM1 pKa = 7.99KK2 pKa = 8.88YY3 pKa = 8.52TLYY6 pKa = 10.73RR7 pKa = 11.84RR8 pKa = 11.84FDD10 pKa = 3.23GTYY13 pKa = 7.89TLLRR17 pKa = 11.84TRR19 pKa = 11.84EE20 pKa = 3.75AWDD23 pKa = 3.45NVLTPIVRR31 pKa = 11.84SNSKK35 pKa = 10.02EE36 pKa = 3.96AILMITEE43 pKa = 4.05DD44 pKa = 3.14VRR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84LEE50 pKa = 3.66

Molecular weight: 6.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

7339

44

839

152.9

17.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.513 ± 0.631

0.559 ± 0.18

6.023 ± 0.264

7.194 ± 0.569

4.088 ± 0.293

6.908 ± 0.691

1.185 ± 0.164

6.677 ± 0.278

8.775 ± 0.441

7.957 ± 0.438

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.338 ± 0.219

5.668 ± 0.259

2.807 ± 0.301

3.733 ± 0.286

4.06 ± 0.375

6.363 ± 0.437

6.009 ± 0.343

6.758 ± 0.34

1.131 ± 0.145

4.251 ± 0.528
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski