Amino acid dipepetide frequency for Rhodococcus phage Harlequin

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.298AlaAla: 10.298 ± 1.262
0.71AlaCys: 0.71 ± 0.254
5.255AlaAsp: 5.255 ± 0.732
9.019AlaGlu: 9.019 ± 0.825
3.125AlaPhe: 3.125 ± 0.423
7.315AlaGly: 7.315 ± 0.835
1.207AlaHis: 1.207 ± 0.332
4.474AlaIle: 4.474 ± 0.819
3.551AlaLys: 3.551 ± 0.471
8.806AlaLeu: 8.806 ± 0.954
2.131AlaMet: 2.131 ± 0.295
3.196AlaAsn: 3.196 ± 0.511
4.261AlaPro: 4.261 ± 0.599
3.267AlaGln: 3.267 ± 0.547
6.037AlaArg: 6.037 ± 0.598
5.184AlaSer: 5.184 ± 0.509
4.687AlaThr: 4.687 ± 0.601
7.457AlaVal: 7.457 ± 0.739
1.562AlaTrp: 1.562 ± 0.362
2.699AlaTyr: 2.699 ± 0.541
0.0AlaXaa: 0.0 ± 0.0
Cys
0.355CysAla: 0.355 ± 0.149
0.0CysCys: 0.0 ± 0.0
0.426CysAsp: 0.426 ± 0.174
0.781CysGlu: 0.781 ± 0.258
0.142CysPhe: 0.142 ± 0.099
1.065CysGly: 1.065 ± 0.258
0.142CysHis: 0.142 ± 0.093
0.213CysIle: 0.213 ± 0.115
0.568CysLys: 0.568 ± 0.225
0.284CysLeu: 0.284 ± 0.131
0.071CysMet: 0.071 ± 0.067
0.284CysAsn: 0.284 ± 0.134
0.71CysPro: 0.71 ± 0.242
0.426CysGln: 0.426 ± 0.17
0.568CysArg: 0.568 ± 0.239
0.426CysSer: 0.426 ± 0.176
0.355CysThr: 0.355 ± 0.153
0.781CysVal: 0.781 ± 0.293
0.142CysTrp: 0.142 ± 0.096
0.426CysTyr: 0.426 ± 0.166
0.0CysXaa: 0.0 ± 0.0
Asp
5.61AspAla: 5.61 ± 0.619
0.639AspCys: 0.639 ± 0.22
2.983AspAsp: 2.983 ± 0.37
5.539AspGlu: 5.539 ± 0.762
2.06AspPhe: 2.06 ± 0.428
5.965AspGly: 5.965 ± 0.761
1.846AspHis: 1.846 ± 0.399
1.207AspIle: 1.207 ± 0.282
2.983AspLys: 2.983 ± 0.421
6.25AspLeu: 6.25 ± 0.719
2.131AspMet: 2.131 ± 0.402
2.202AspAsn: 2.202 ± 0.399
5.042AspPro: 5.042 ± 0.767
2.202AspGln: 2.202 ± 0.382
3.125AspArg: 3.125 ± 0.419
3.622AspSer: 3.622 ± 0.456
3.622AspThr: 3.622 ± 0.428
5.184AspVal: 5.184 ± 0.599
1.278AspTrp: 1.278 ± 0.354
2.628AspTyr: 2.628 ± 0.553
0.0AspXaa: 0.0 ± 0.0
Glu
7.812GluAla: 7.812 ± 0.807
0.568GluCys: 0.568 ± 0.184
5.184GluAsp: 5.184 ± 0.8
4.971GluGlu: 4.971 ± 0.697
2.557GluPhe: 2.557 ± 0.503
6.25GluGly: 6.25 ± 0.687
1.42GluHis: 1.42 ± 0.354
4.829GluIle: 4.829 ± 0.788
2.415GluLys: 2.415 ± 0.487
7.315GluLeu: 7.315 ± 0.814
1.349GluMet: 1.349 ± 0.295
2.841GluAsn: 2.841 ± 0.45
2.415GluPro: 2.415 ± 0.365
2.486GluGln: 2.486 ± 0.441
4.261GluArg: 4.261 ± 0.668
3.409GluSer: 3.409 ± 0.543
4.332GluThr: 4.332 ± 0.585
6.605GluVal: 6.605 ± 0.86
1.42GluTrp: 1.42 ± 0.295
1.917GluTyr: 1.917 ± 0.334
0.0GluXaa: 0.0 ± 0.0
Phe
3.409PheAla: 3.409 ± 0.511
0.213PheCys: 0.213 ± 0.123
2.841PheAsp: 2.841 ± 0.506
2.415PheGlu: 2.415 ± 0.47
0.639PhePhe: 0.639 ± 0.185
2.77PheGly: 2.77 ± 0.441
0.71PheHis: 0.71 ± 0.258
1.491PheIle: 1.491 ± 0.268
1.349PheLys: 1.349 ± 0.287
2.983PheLeu: 2.983 ± 0.538
0.639PheMet: 0.639 ± 0.18
1.207PheAsn: 1.207 ± 0.226
1.278PhePro: 1.278 ± 0.258
1.136PheGln: 1.136 ± 0.273
2.344PheArg: 2.344 ± 0.381
2.486PheSer: 2.486 ± 0.362
1.775PheThr: 1.775 ± 0.31
2.699PheVal: 2.699 ± 0.449
0.497PheTrp: 0.497 ± 0.155
1.065PheTyr: 1.065 ± 0.293
0.0PheXaa: 0.0 ± 0.0
Gly
6.534GlyAla: 6.534 ± 0.917
0.639GlyCys: 0.639 ± 0.252
6.676GlyAsp: 6.676 ± 0.769
5.184GlyGlu: 5.184 ± 0.612
4.545GlyPhe: 4.545 ± 0.65
6.25GlyGly: 6.25 ± 0.608
1.988GlyHis: 1.988 ± 0.348
4.119GlyIle: 4.119 ± 0.622
4.119GlyLys: 4.119 ± 0.406
5.61GlyLeu: 5.61 ± 0.799
2.273GlyMet: 2.273 ± 0.641
2.983GlyAsn: 2.983 ± 0.464
3.551GlyPro: 3.551 ± 0.398
3.125GlyGln: 3.125 ± 0.603
3.977GlyArg: 3.977 ± 0.674
6.037GlySer: 6.037 ± 1.076
5.539GlyThr: 5.539 ± 0.741
6.889GlyVal: 6.889 ± 0.664
2.202GlyTrp: 2.202 ± 0.475
2.983GlyTyr: 2.983 ± 0.545
0.0GlyXaa: 0.0 ± 0.0
His
1.562HisAla: 1.562 ± 0.346
0.142HisCys: 0.142 ± 0.106
1.562HisAsp: 1.562 ± 0.315
1.633HisGlu: 1.633 ± 0.356
0.71HisPhe: 0.71 ± 0.242
1.278HisGly: 1.278 ± 0.3
0.568HisHis: 0.568 ± 0.211
1.42HisIle: 1.42 ± 0.375
0.568HisLys: 0.568 ± 0.199
1.42HisLeu: 1.42 ± 0.374
0.639HisMet: 0.639 ± 0.272
0.71HisAsn: 0.71 ± 0.223
1.065HisPro: 1.065 ± 0.262
0.71HisGln: 0.71 ± 0.193
1.775HisArg: 1.775 ± 0.398
0.781HisSer: 0.781 ± 0.32
0.994HisThr: 0.994 ± 0.263
1.278HisVal: 1.278 ± 0.287
0.426HisTrp: 0.426 ± 0.196
0.781HisTyr: 0.781 ± 0.225
0.0HisXaa: 0.0 ± 0.0
Ile
5.184IleAla: 5.184 ± 0.732
0.355IleCys: 0.355 ± 0.186
3.551IleAsp: 3.551 ± 0.569
3.622IleGlu: 3.622 ± 0.529
0.923IlePhe: 0.923 ± 0.275
4.9IleGly: 4.9 ± 0.707
1.065IleHis: 1.065 ± 0.255
1.491IleIle: 1.491 ± 0.308
1.136IleLys: 1.136 ± 0.297
3.196IleLeu: 3.196 ± 0.585
0.639IleMet: 0.639 ± 0.191
1.42IleAsn: 1.42 ± 0.295
2.77IlePro: 2.77 ± 0.482
0.852IleGln: 0.852 ± 0.26
3.622IleArg: 3.622 ± 0.477
1.988IleSer: 1.988 ± 0.392
3.906IleThr: 3.906 ± 0.57
3.48IleVal: 3.48 ± 0.599
0.426IleTrp: 0.426 ± 0.208
1.491IleTyr: 1.491 ± 0.394
0.0IleXaa: 0.0 ± 0.0
Lys
3.906LysAla: 3.906 ± 0.539
0.142LysCys: 0.142 ± 0.089
2.344LysAsp: 2.344 ± 0.328
1.846LysGlu: 1.846 ± 0.331
1.207LysPhe: 1.207 ± 0.341
3.764LysGly: 3.764 ± 0.825
1.065LysHis: 1.065 ± 0.252
1.491LysIle: 1.491 ± 0.327
1.917LysLys: 1.917 ± 0.413
4.332LysLeu: 4.332 ± 0.627
0.781LysMet: 0.781 ± 0.311
1.349LysAsn: 1.349 ± 0.345
1.562LysPro: 1.562 ± 0.348
0.781LysGln: 0.781 ± 0.204
2.273LysArg: 2.273 ± 0.407
2.202LysSer: 2.202 ± 0.279
2.557LysThr: 2.557 ± 0.571
3.551LysVal: 3.551 ± 0.573
0.781LysTrp: 0.781 ± 0.208
0.994LysTyr: 0.994 ± 0.246
0.0LysXaa: 0.0 ± 0.0
Leu
9.445LeuAla: 9.445 ± 0.859
0.568LeuCys: 0.568 ± 0.202
5.823LeuAsp: 5.823 ± 0.649
6.108LeuGlu: 6.108 ± 0.883
2.415LeuPhe: 2.415 ± 0.349
6.676LeuGly: 6.676 ± 0.928
1.775LeuHis: 1.775 ± 0.411
3.054LeuIle: 3.054 ± 0.473
3.551LeuLys: 3.551 ± 0.548
5.113LeuLeu: 5.113 ± 0.612
1.775LeuMet: 1.775 ± 0.443
2.486LeuAsn: 2.486 ± 0.469
4.474LeuPro: 4.474 ± 0.576
2.841LeuGln: 2.841 ± 0.425
5.397LeuArg: 5.397 ± 0.778
5.326LeuSer: 5.326 ± 0.629
6.179LeuThr: 6.179 ± 0.698
6.321LeuVal: 6.321 ± 0.644
1.562LeuTrp: 1.562 ± 0.344
2.557LeuTyr: 2.557 ± 0.357
0.0LeuXaa: 0.0 ± 0.0
Met
1.917MetAla: 1.917 ± 0.407
0.0MetCys: 0.0 ± 0.0
1.207MetAsp: 1.207 ± 0.275
1.349MetGlu: 1.349 ± 0.312
0.852MetPhe: 0.852 ± 0.265
2.202MetGly: 2.202 ± 0.291
0.355MetHis: 0.355 ± 0.164
1.562MetIle: 1.562 ± 0.347
1.136MetLys: 1.136 ± 0.329
1.704MetLeu: 1.704 ± 0.269
0.355MetMet: 0.355 ± 0.16
0.852MetAsn: 0.852 ± 0.216
0.781MetPro: 0.781 ± 0.238
0.497MetGln: 0.497 ± 0.196
1.775MetArg: 1.775 ± 0.431
3.054MetSer: 3.054 ± 0.451
2.06MetThr: 2.06 ± 0.37
1.207MetVal: 1.207 ± 0.288
0.213MetTrp: 0.213 ± 0.12
0.568MetTyr: 0.568 ± 0.262
0.0MetXaa: 0.0 ± 0.0
Asn
3.267AsnAla: 3.267 ± 0.434
0.639AsnCys: 0.639 ± 0.277
2.202AsnAsp: 2.202 ± 0.371
2.06AsnGlu: 2.06 ± 0.353
1.065AsnPhe: 1.065 ± 0.31
3.906AsnGly: 3.906 ± 0.557
0.923AsnHis: 0.923 ± 0.262
1.349AsnIle: 1.349 ± 0.25
0.994AsnLys: 0.994 ± 0.232
3.125AsnLeu: 3.125 ± 0.43
1.065AsnMet: 1.065 ± 0.3
1.491AsnAsn: 1.491 ± 0.304
2.131AsnPro: 2.131 ± 0.365
0.852AsnGln: 0.852 ± 0.226
1.633AsnArg: 1.633 ± 0.385
2.202AsnSer: 2.202 ± 0.416
2.202AsnThr: 2.202 ± 0.428
2.486AsnVal: 2.486 ± 0.351
0.852AsnTrp: 0.852 ± 0.25
1.207AsnTyr: 1.207 ± 0.386
0.0AsnXaa: 0.0 ± 0.0
Pro
4.19ProAla: 4.19 ± 0.459
0.355ProCys: 0.355 ± 0.193
2.77ProAsp: 2.77 ± 0.377
3.409ProGlu: 3.409 ± 0.495
1.278ProPhe: 1.278 ± 0.213
4.403ProGly: 4.403 ± 0.721
0.71ProHis: 0.71 ± 0.231
2.841ProIle: 2.841 ± 0.495
1.42ProLys: 1.42 ± 0.314
3.693ProLeu: 3.693 ± 0.477
1.42ProMet: 1.42 ± 0.274
2.202ProAsn: 2.202 ± 0.424
1.278ProPro: 1.278 ± 0.311
1.491ProGln: 1.491 ± 0.262
2.77ProArg: 2.77 ± 0.57
2.273ProSer: 2.273 ± 0.33
3.764ProThr: 3.764 ± 0.497
4.119ProVal: 4.119 ± 0.596
1.136ProTrp: 1.136 ± 0.246
1.42ProTyr: 1.42 ± 0.268
0.0ProXaa: 0.0 ± 0.0
Gln
3.054GlnAla: 3.054 ± 0.436
0.142GlnCys: 0.142 ± 0.163
2.06GlnAsp: 2.06 ± 0.383
1.491GlnGlu: 1.491 ± 0.382
1.065GlnPhe: 1.065 ± 0.273
2.486GlnGly: 2.486 ± 0.483
0.639GlnHis: 0.639 ± 0.22
1.846GlnIle: 1.846 ± 0.375
0.923GlnLys: 0.923 ± 0.259
2.699GlnLeu: 2.699 ± 0.508
0.852GlnMet: 0.852 ± 0.229
0.71GlnAsn: 0.71 ± 0.179
1.775GlnPro: 1.775 ± 0.322
1.278GlnGln: 1.278 ± 0.344
2.912GlnArg: 2.912 ± 0.408
1.917GlnSer: 1.917 ± 0.305
1.775GlnThr: 1.775 ± 0.347
3.409GlnVal: 3.409 ± 0.47
0.639GlnTrp: 0.639 ± 0.212
0.852GlnTyr: 0.852 ± 0.246
0.0GlnXaa: 0.0 ± 0.0
Arg
5.184ArgAla: 5.184 ± 0.601
1.207ArgCys: 1.207 ± 0.311
4.119ArgAsp: 4.119 ± 0.502
4.971ArgGlu: 4.971 ± 0.722
2.486ArgPhe: 2.486 ± 0.437
3.764ArgGly: 3.764 ± 0.419
1.207ArgHis: 1.207 ± 0.423
2.557ArgIle: 2.557 ± 0.472
2.77ArgLys: 2.77 ± 0.368
5.326ArgLeu: 5.326 ± 0.579
0.994ArgMet: 0.994 ± 0.277
2.273ArgAsn: 2.273 ± 0.448
2.699ArgPro: 2.699 ± 0.491
2.486ArgGln: 2.486 ± 0.405
3.835ArgArg: 3.835 ± 0.519
3.409ArgSer: 3.409 ± 0.49
4.048ArgThr: 4.048 ± 0.605
4.403ArgVal: 4.403 ± 0.506
1.349ArgTrp: 1.349 ± 0.349
1.775ArgTyr: 1.775 ± 0.404
0.0ArgXaa: 0.0 ± 0.0
Ser
5.042SerAla: 5.042 ± 0.569
0.568SerCys: 0.568 ± 0.185
3.764SerAsp: 3.764 ± 0.374
4.261SerGlu: 4.261 ± 0.7
2.699SerPhe: 2.699 ± 0.501
6.037SerGly: 6.037 ± 0.785
1.136SerHis: 1.136 ± 0.274
2.202SerIle: 2.202 ± 0.365
2.273SerLys: 2.273 ± 0.516
5.113SerLeu: 5.113 ± 0.601
1.846SerMet: 1.846 ± 0.388
2.699SerAsn: 2.699 ± 0.424
2.344SerPro: 2.344 ± 0.428
1.775SerGln: 1.775 ± 0.303
3.622SerArg: 3.622 ± 0.458
3.551SerSer: 3.551 ± 0.537
3.196SerThr: 3.196 ± 0.385
4.545SerVal: 4.545 ± 0.677
1.207SerTrp: 1.207 ± 0.316
1.775SerTyr: 1.775 ± 0.346
0.0SerXaa: 0.0 ± 0.0
Thr
4.971ThrAla: 4.971 ± 0.562
0.355ThrCys: 0.355 ± 0.164
3.409ThrAsp: 3.409 ± 0.416
5.042ThrGlu: 5.042 ± 0.547
1.846ThrPhe: 1.846 ± 0.387
6.037ThrGly: 6.037 ± 0.834
1.065ThrHis: 1.065 ± 0.259
3.409ThrIle: 3.409 ± 0.446
2.628ThrLys: 2.628 ± 0.412
5.539ThrLeu: 5.539 ± 0.506
1.278ThrMet: 1.278 ± 0.313
1.562ThrAsn: 1.562 ± 0.405
3.764ThrPro: 3.764 ± 0.512
1.917ThrGln: 1.917 ± 0.34
3.338ThrArg: 3.338 ± 0.471
2.912ThrSer: 2.912 ± 0.491
3.693ThrThr: 3.693 ± 0.655
4.971ThrVal: 4.971 ± 0.554
1.136ThrTrp: 1.136 ± 0.215
2.557ThrTyr: 2.557 ± 0.42
0.0ThrXaa: 0.0 ± 0.0
Val
7.812ValAla: 7.812 ± 0.704
0.781ValCys: 0.781 ± 0.218
5.681ValAsp: 5.681 ± 0.586
6.605ValGlu: 6.605 ± 0.716
2.699ValPhe: 2.699 ± 0.452
5.894ValGly: 5.894 ± 0.676
1.065ValHis: 1.065 ± 0.254
3.764ValIle: 3.764 ± 0.462
3.125ValLys: 3.125 ± 0.489
7.173ValLeu: 7.173 ± 0.661
1.633ValMet: 1.633 ± 0.428
3.125ValAsn: 3.125 ± 0.348
3.764ValPro: 3.764 ± 0.505
2.344ValGln: 2.344 ± 0.43
4.403ValArg: 4.403 ± 0.662
5.255ValSer: 5.255 ± 0.586
4.19ValThr: 4.19 ± 0.648
6.179ValVal: 6.179 ± 0.771
1.349ValTrp: 1.349 ± 0.364
2.131ValTyr: 2.131 ± 0.421
0.0ValXaa: 0.0 ± 0.0
Trp
1.349TrpAla: 1.349 ± 0.259
0.0TrpCys: 0.0 ± 0.0
1.42TrpAsp: 1.42 ± 0.294
1.42TrpGlu: 1.42 ± 0.234
0.71TrpPhe: 0.71 ± 0.226
1.633TrpGly: 1.633 ± 0.396
0.639TrpHis: 0.639 ± 0.203
0.923TrpIle: 0.923 ± 0.251
0.852TrpLys: 0.852 ± 0.236
1.278TrpLeu: 1.278 ± 0.361
0.639TrpMet: 0.639 ± 0.193
0.923TrpAsn: 0.923 ± 0.291
0.639TrpPro: 0.639 ± 0.229
0.781TrpGln: 0.781 ± 0.225
0.497TrpArg: 0.497 ± 0.168
1.42TrpSer: 1.42 ± 0.353
1.207TrpThr: 1.207 ± 0.271
1.349TrpVal: 1.349 ± 0.266
0.355TrpTrp: 0.355 ± 0.194
0.71TrpTyr: 0.71 ± 0.254
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.338TyrAla: 3.338 ± 0.533
0.142TyrCys: 0.142 ± 0.111
2.841TyrAsp: 2.841 ± 0.424
2.628TyrGlu: 2.628 ± 0.465
0.923TyrPhe: 0.923 ± 0.262
2.628TyrGly: 2.628 ± 0.513
0.497TyrHis: 0.497 ± 0.166
1.775TyrIle: 1.775 ± 0.345
0.639TyrLys: 0.639 ± 0.238
2.486TyrLeu: 2.486 ± 0.513
1.065TyrMet: 1.065 ± 0.218
1.136TyrAsn: 1.136 ± 0.321
0.639TyrPro: 0.639 ± 0.194
1.278TyrGln: 1.278 ± 0.252
2.699TyrArg: 2.699 ± 0.489
2.202TyrSer: 2.202 ± 0.439
1.349TyrThr: 1.349 ± 0.321
2.06TyrVal: 2.06 ± 0.473
0.213TyrTrp: 0.213 ± 0.124
0.639TyrTyr: 0.639 ± 0.206
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (14082 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski