Rhodococcus phage Gollum

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Rerduovirus; unclassified Rerduovirus

Average proteome isoelectric point is 5.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4Q4R2|A0A2Z4Q4R2_9CAUD Ribonucleoside-triphosphate reductase (thioredoxin) OS=Rhodococcus phage Gollum OX=2201450 GN=41 PE=3 SV=1
MM1 pKa = 7.64IEE3 pKa = 4.26SKK5 pKa = 10.99SLGEE9 pKa = 3.86VAGFEE14 pKa = 4.52IEE16 pKa = 4.77AGWIYY21 pKa = 11.35DD22 pKa = 3.59DD23 pKa = 5.01VGPDD27 pKa = 3.51FYY29 pKa = 11.57GDD31 pKa = 3.54EE32 pKa = 4.51VYY34 pKa = 11.07DD35 pKa = 4.0DD36 pKa = 3.87QTIEE40 pKa = 4.25AYY42 pKa = 9.81EE43 pKa = 3.88NNEE46 pKa = 3.39WHH48 pKa = 6.38FVVLIVEE55 pKa = 4.27ARR57 pKa = 11.84FNGHH61 pKa = 5.81VMGTAYY67 pKa = 10.87LGGVEE72 pKa = 4.51VGFFPGVAEE81 pKa = 4.48PLDD84 pKa = 4.03PLTNSDD90 pKa = 4.46YY91 pKa = 11.04LDD93 pKa = 4.2DD94 pKa = 4.97MIAEE98 pKa = 4.68AVNSARR104 pKa = 11.84TEE106 pKa = 3.99LQEE109 pKa = 4.41TITKK113 pKa = 9.99GLEE116 pKa = 3.77VLLL119 pKa = 4.65

Molecular weight: 13.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4Q4X5|A0A2Z4Q4X5_9CAUD Uncharacterized protein OS=Rhodococcus phage Gollum OX=2201450 GN=69 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.61NKK4 pKa = 10.22GLTQSEE10 pKa = 4.3IAKK13 pKa = 9.94QYY15 pKa = 10.71GVTRR19 pKa = 11.84QYY21 pKa = 11.32VSWIIKK27 pKa = 9.13TYY29 pKa = 10.87GGTKK33 pKa = 8.95TPRR36 pKa = 11.84QKK38 pKa = 10.16MLEE41 pKa = 4.08HH42 pKa = 6.5FPWEE46 pKa = 4.46VPVEE50 pKa = 4.16MNPQSPYY57 pKa = 10.62RR58 pKa = 11.84NMRR61 pKa = 11.84NHH63 pKa = 7.15AEE65 pKa = 3.94YY66 pKa = 9.09MATGGVGMDD75 pKa = 3.38KK76 pKa = 11.06GAGVRR81 pKa = 11.84SEE83 pKa = 4.45HH84 pKa = 6.33PRR86 pKa = 11.84HH87 pKa = 4.96TRR89 pKa = 11.84FRR91 pKa = 11.84QARR94 pKa = 11.84WVCAAPKK101 pKa = 10.04GRR103 pKa = 11.84GRR105 pKa = 11.84RR106 pKa = 11.84GSDD109 pKa = 3.36DD110 pKa = 3.5PSEE113 pKa = 4.05

Molecular weight: 12.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

14047

42

1115

212.8

23.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.468 ± 0.357

0.847 ± 0.121

6.599 ± 0.188

7.318 ± 0.495

3.374 ± 0.166

8.472 ± 0.393

1.958 ± 0.172

4.563 ± 0.176

3.937 ± 0.182

8.279 ± 0.314

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.356 ± 0.173

3.488 ± 0.202

4.691 ± 0.236

3.36 ± 0.17

6.122 ± 0.281

6.072 ± 0.231

6.144 ± 0.28

7.938 ± 0.259

1.887 ± 0.161

3.125 ± 0.231
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski