Hymenobacter sp. BRD67

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Hymenobacter; unclassified Hymenobacter

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3905 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D4BBL4|A0A7D4BBL4_9BACT Pyridoxal phosphate-dependent aminotransferase OS=Hymenobacter sp. BRD67 OX=2675877 GN=GKZ67_04695 PE=4 SV=1
MM1 pKa = 7.8LSTTDD6 pKa = 3.56YY7 pKa = 9.79TALPPAADD15 pKa = 3.91LQRR18 pKa = 11.84LCQALAALDD27 pKa = 3.99AVNSPDD33 pKa = 3.04EE34 pKa = 4.16EE35 pKa = 4.11YY36 pKa = 10.61RR37 pKa = 11.84YY38 pKa = 10.19FSYY41 pKa = 10.95NPEE44 pKa = 3.66WSEE47 pKa = 3.88NEE49 pKa = 3.87AAFEE53 pKa = 4.46LNDD56 pKa = 4.22GEE58 pKa = 4.75GDD60 pKa = 3.45QLLVLFRR67 pKa = 11.84PEE69 pKa = 3.93GCVMNGFLAGYY80 pKa = 7.41DD81 pKa = 3.74QPDD84 pKa = 3.62KK85 pKa = 11.44ALATHH90 pKa = 6.84GLPAAFEE97 pKa = 4.05EE98 pKa = 5.0FMFGEE103 pKa = 4.8PVASIGTTFCLWYY116 pKa = 9.47TPVQGWQGGAADD128 pKa = 4.33GSEE131 pKa = 4.3EE132 pKa = 4.72LLFMLDD138 pKa = 3.88NQPATYY144 pKa = 10.08AAWATEE150 pKa = 4.4YY151 pKa = 9.89YY152 pKa = 10.19TEE154 pKa = 4.19EE155 pKa = 4.12TDD157 pKa = 3.71KK158 pKa = 11.55KK159 pKa = 9.5PVSASAVAPVYY170 pKa = 9.98RR171 pKa = 11.84HH172 pKa = 5.72EE173 pKa = 4.24VLTRR177 pKa = 11.84ARR179 pKa = 11.84VLALVDD185 pKa = 4.41EE186 pKa = 5.09LEE188 pKa = 4.91DD189 pKa = 3.84WPQLAADD196 pKa = 4.88LQQIGYY202 pKa = 8.3PYY204 pKa = 10.71DD205 pKa = 3.55FEE207 pKa = 4.99GVV209 pKa = 3.28

Molecular weight: 23.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D3WQX6|A0A7D3WQX6_9BACT DUF4252 domain-containing protein OS=Hymenobacter sp. BRD67 OX=2675877 GN=GKZ67_00420 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 10.28LATVFLLSTIKK13 pKa = 10.69FLGGPLAGSKK23 pKa = 9.27MGLGFWPTLGLTVAGMMTSVFIISGVGRR51 pKa = 11.84TWVLHH56 pKa = 5.25RR57 pKa = 11.84QQQRR61 pKa = 11.84RR62 pKa = 11.84LRR64 pKa = 11.84RR65 pKa = 11.84VPVFSKK71 pKa = 10.48RR72 pKa = 11.84SRR74 pKa = 11.84NIIKK78 pKa = 9.39VFKK81 pKa = 9.58RR82 pKa = 11.84FGMGGIAFLTPVLLSPIGGTVIATLLGVPRR112 pKa = 11.84QRR114 pKa = 11.84ILLHH118 pKa = 5.58MLWSAVLWGGVLTLASTRR136 pKa = 11.84IGHH139 pKa = 5.87MLHH142 pKa = 6.73HH143 pKa = 7.27

Molecular weight: 15.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3905

0

3905

1139927

34

2524

291.9

31.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.323 ± 0.053

0.778 ± 0.012

4.658 ± 0.031

4.79 ± 0.049

3.882 ± 0.024

7.852 ± 0.049

2.11 ± 0.02

4.142 ± 0.038

3.684 ± 0.048

11.181 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.739 ± 0.021

3.614 ± 0.037

5.524 ± 0.034

4.722 ± 0.033

6.105 ± 0.044

5.738 ± 0.043

6.5 ± 0.063

6.926 ± 0.035

1.255 ± 0.017

3.477 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski