Ajellomyces capsulatus (strain G186AR / H82 / ATCC MYA-2454 / RMSCC 2432) (Darling s disease fungus) (Histoplasma capsulatum)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Onygenales; Ajellomycetaceae; Histoplasma; Histoplasma capsulatum

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9214 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C0NLH3|C0NLH3_AJECG MFS transporter OS=Ajellomyces capsulatus (strain G186AR / H82 / ATCC MYA-2454 / RMSCC 2432) OX=447093 GN=HCBG_04353 PE=4 SV=1
MM1 pKa = 6.53VTYY4 pKa = 10.51ILYY7 pKa = 9.99GFRR10 pKa = 11.84WNRR13 pKa = 11.84AANPLAPGIRR23 pKa = 11.84AYY25 pKa = 8.82ITLCNILDD33 pKa = 3.83AAAEE37 pKa = 4.16YY38 pKa = 9.9LQHH41 pKa = 7.2PSTTTAVLNSFKK53 pKa = 10.99LIDD56 pKa = 4.09SNILTHH62 pKa = 7.2LPDD65 pKa = 4.53LEE67 pKa = 5.99LIEE70 pKa = 5.0QYY72 pKa = 11.14DD73 pKa = 4.17PEE75 pKa = 5.37DD76 pKa = 3.77LSADD80 pKa = 3.56AVSQPYY86 pKa = 10.3AYY88 pKa = 10.29VAAKK92 pKa = 9.27TMTMGANALSGAGLGLSLQDD112 pKa = 5.16ILQQDD117 pKa = 4.11PGLSTAGTDD126 pKa = 3.42VFKK129 pKa = 11.11KK130 pKa = 10.59LRR132 pKa = 11.84DD133 pKa = 3.5EE134 pKa = 4.59LAPDD138 pKa = 3.95SEE140 pKa = 4.57IGWFVVYY147 pKa = 10.82NGDD150 pKa = 3.53PEE152 pKa = 4.06RR153 pKa = 11.84SYY155 pKa = 11.92GSFYY159 pKa = 10.85GDD161 pKa = 3.2SAVEE165 pKa = 3.99SDD167 pKa = 3.55GG168 pKa = 4.2

Molecular weight: 18.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C0NPA9|C0NPA9_AJECG Uncharacterized protein OS=Ajellomyces capsulatus (strain G186AR / H82 / ATCC MYA-2454 / RMSCC 2432) OX=447093 GN=HCBG_04989 PE=4 SV=1
MM1 pKa = 7.29IAGFFFICWRR11 pKa = 11.84IGQLVTLIIPIGILSWFVDD30 pKa = 3.63GFVKK34 pKa = 10.62NNQLTPTYY42 pKa = 9.91ILVLFIVSVLGIFWALDD59 pKa = 3.12TLIRR63 pKa = 11.84HH64 pKa = 5.9SNAKK68 pKa = 9.99RR69 pKa = 11.84SAHH72 pKa = 5.27FVAFVDD78 pKa = 3.95LCFVGSFIAGVYY90 pKa = 6.8QLRR93 pKa = 11.84RR94 pKa = 11.84IANADD99 pKa = 3.37CGNFRR104 pKa = 11.84FDD106 pKa = 4.89PLTLSLGPFGFAGQSANNPLARR128 pKa = 11.84DD129 pKa = 3.84PTKK132 pKa = 10.66VCAMLKK138 pKa = 9.33TSFAFGIMNIGSFFITSILAVLMHH162 pKa = 5.97HH163 pKa = 6.36HH164 pKa = 6.59EE165 pKa = 4.29EE166 pKa = 4.32RR167 pKa = 11.84EE168 pKa = 4.17HH169 pKa = 6.45EE170 pKa = 4.41KK171 pKa = 10.69GSSRR175 pKa = 11.84RR176 pKa = 11.84RR177 pKa = 11.84GSHH180 pKa = 5.08SSHH183 pKa = 6.83RR184 pKa = 11.84GHH186 pKa = 6.32SGSRR190 pKa = 11.84HH191 pKa = 4.98RR192 pKa = 11.84RR193 pKa = 11.84SSSGRR198 pKa = 11.84QPAYY202 pKa = 10.44AVV204 pKa = 3.25

Molecular weight: 22.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9214

0

9214

4155681

39

5891

451.0

50.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.9 ± 0.021

1.265 ± 0.01

5.545 ± 0.017

6.129 ± 0.024

3.639 ± 0.017

6.754 ± 0.021

2.5 ± 0.011

5.105 ± 0.018

5.002 ± 0.024

8.864 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.087 ± 0.008

3.954 ± 0.014

6.296 ± 0.031

4.073 ± 0.019

6.446 ± 0.024

8.822 ± 0.033

5.857 ± 0.014

5.802 ± 0.017

1.328 ± 0.009

2.63 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski