Amino acid dipepetide frequency for Flavobacteria bacterium MS024-3C

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.49AlaAla: 7.49 ± 0.199
0.616AlaCys: 0.616 ± 0.042
3.49AlaAsp: 3.49 ± 0.111
3.872AlaGlu: 3.872 ± 0.101
4.153AlaPhe: 4.153 ± 0.097
5.063AlaGly: 5.063 ± 0.125
1.681AlaHis: 1.681 ± 0.057
6.002AlaIle: 6.002 ± 0.138
5.174AlaLys: 5.174 ± 0.13
9.024AlaLeu: 9.024 ± 0.152
2.031AlaMet: 2.031 ± 0.08
3.522AlaAsn: 3.522 ± 0.094
2.921AlaPro: 2.921 ± 0.084
3.761AlaGln: 3.761 ± 0.098
2.284AlaArg: 2.284 ± 0.08
5.259AlaSer: 5.259 ± 0.147
4.517AlaThr: 4.517 ± 0.14
5.448AlaVal: 5.448 ± 0.138
0.801AlaTrp: 0.801 ± 0.045
3.092AlaTyr: 3.092 ± 0.075
0.0AlaXaa: 0.0 ± 0.0
Cys
0.509CysAla: 0.509 ± 0.032
0.109CysCys: 0.109 ± 0.017
0.373CysAsp: 0.373 ± 0.031
0.43CysGlu: 0.43 ± 0.037
0.396CysPhe: 0.396 ± 0.031
0.648CysGly: 0.648 ± 0.037
0.166CysHis: 0.166 ± 0.02
0.552CysIle: 0.552 ± 0.033
0.328CysLys: 0.328 ± 0.029
0.65CysLeu: 0.65 ± 0.04
0.158CysMet: 0.158 ± 0.019
0.324CysAsn: 0.324 ± 0.028
0.317CysPro: 0.317 ± 0.03
0.237CysGln: 0.237 ± 0.025
0.151CysArg: 0.151 ± 0.017
0.503CysSer: 0.503 ± 0.037
0.418CysThr: 0.418 ± 0.039
0.479CysVal: 0.479 ± 0.032
0.051CysTrp: 0.051 ± 0.01
0.258CysTyr: 0.258 ± 0.026
0.0CysXaa: 0.0 ± 0.0
Asp
4.311AspAla: 4.311 ± 0.12
0.328AspCys: 0.328 ± 0.027
1.581AspAsp: 1.581 ± 0.127
2.397AspGlu: 2.397 ± 0.081
3.497AspPhe: 3.497 ± 0.091
3.567AspGly: 3.567 ± 0.169
1.272AspHis: 1.272 ± 0.061
3.906AspIle: 3.906 ± 0.097
3.311AspLys: 3.311 ± 0.09
5.698AspLeu: 5.698 ± 0.124
1.093AspMet: 1.093 ± 0.049
2.536AspAsn: 2.536 ± 0.124
2.425AspPro: 2.425 ± 0.072
2.408AspGln: 2.408 ± 0.071
1.941AspArg: 1.941 ± 0.061
2.913AspSer: 2.913 ± 0.097
2.798AspThr: 2.798 ± 0.139
2.97AspVal: 2.97 ± 0.087
0.714AspTrp: 0.714 ± 0.04
2.51AspTyr: 2.51 ± 0.088
0.0AspXaa: 0.0 ± 0.0
Glu
5.478GluAla: 5.478 ± 0.13
0.209GluCys: 0.209 ± 0.022
3.422GluAsp: 3.422 ± 0.094
4.025GluGlu: 4.025 ± 0.121
2.203GluPhe: 2.203 ± 0.075
3.936GluGly: 3.936 ± 0.1
0.938GluHis: 0.938 ± 0.047
4.816GluIle: 4.816 ± 0.111
5.289GluLys: 5.289 ± 0.142
4.997GluLeu: 4.997 ± 0.115
1.579GluMet: 1.579 ± 0.059
3.771GluAsn: 3.771 ± 0.111
1.4GluPro: 1.4 ± 0.069
2.116GluGln: 2.116 ± 0.071
2.348GluArg: 2.348 ± 0.093
3.2GluSer: 3.2 ± 0.087
3.588GluThr: 3.588 ± 0.096
4.624GluVal: 4.624 ± 0.097
0.59GluTrp: 0.59 ± 0.037
1.796GluTyr: 1.796 ± 0.068
0.0GluXaa: 0.0 ± 0.0
Phe
3.079PheAla: 3.079 ± 0.082
0.405PheCys: 0.405 ± 0.033
2.642PheAsp: 2.642 ± 0.082
3.26PheGlu: 3.26 ± 0.084
2.787PhePhe: 2.787 ± 0.099
3.769PheGly: 3.769 ± 0.096
0.859PheHis: 0.859 ± 0.043
3.52PheIle: 3.52 ± 0.1
3.703PheLys: 3.703 ± 0.096
4.888PheLeu: 4.888 ± 0.124
1.244PheMet: 1.244 ± 0.049
3.171PheAsn: 3.171 ± 0.108
1.837PhePro: 1.837 ± 0.059
1.494PheGln: 1.494 ± 0.059
1.602PheArg: 1.602 ± 0.056
4.136PheSer: 4.136 ± 0.106
2.821PheThr: 2.821 ± 0.099
3.149PheVal: 3.149 ± 0.089
0.599PheTrp: 0.599 ± 0.037
1.905PheTyr: 1.905 ± 0.074
0.0PheXaa: 0.0 ± 0.0
Gly
5.774GlyAla: 5.774 ± 0.14
0.586GlyCys: 0.586 ± 0.04
3.582GlyAsp: 3.582 ± 0.118
3.269GlyGlu: 3.269 ± 0.085
3.912GlyPhe: 3.912 ± 0.103
5.229GlyGly: 5.229 ± 0.147
1.374GlyHis: 1.374 ± 0.06
5.612GlyIle: 5.612 ± 0.111
4.432GlyLys: 4.432 ± 0.098
6.908GlyLeu: 6.908 ± 0.148
1.815GlyMet: 1.815 ± 0.067
3.273GlyAsn: 3.273 ± 0.106
1.903GlyPro: 1.903 ± 0.072
2.288GlyGln: 2.288 ± 0.076
2.212GlyArg: 2.212 ± 0.079
4.549GlySer: 4.549 ± 0.124
4.125GlyThr: 4.125 ± 0.158
5.365GlyVal: 5.365 ± 0.13
0.803GlyTrp: 0.803 ± 0.051
2.73GlyTyr: 2.73 ± 0.093
0.0GlyXaa: 0.0 ± 0.0
His
1.272HisAla: 1.272 ± 0.053
0.207HisCys: 0.207 ± 0.021
0.795HisAsp: 0.795 ± 0.048
1.059HisGlu: 1.059 ± 0.05
1.257HisPhe: 1.257 ± 0.058
1.289HisGly: 1.289 ± 0.062
0.52HisHis: 0.52 ± 0.038
1.421HisIle: 1.421 ± 0.064
1.272HisLys: 1.272 ± 0.056
2.216HisLeu: 2.216 ± 0.082
0.458HisMet: 0.458 ± 0.027
0.793HisAsn: 0.793 ± 0.042
1.076HisPro: 1.076 ± 0.046
0.769HisGln: 0.769 ± 0.037
0.733HisArg: 0.733 ± 0.044
1.217HisSer: 1.217 ± 0.053
0.955HisThr: 0.955 ± 0.047
0.989HisVal: 0.989 ± 0.05
0.298HisTrp: 0.298 ± 0.025
0.869HisTyr: 0.869 ± 0.043
0.0HisXaa: 0.0 ± 0.0
Ile
6.034IleAla: 6.034 ± 0.133
0.609IleCys: 0.609 ± 0.039
4.551IleAsp: 4.551 ± 0.097
4.718IleGlu: 4.718 ± 0.11
3.22IlePhe: 3.22 ± 0.108
5.303IleGly: 5.303 ± 0.115
1.398IleHis: 1.398 ± 0.059
4.594IleIle: 4.594 ± 0.118
4.839IleLys: 4.839 ± 0.11
6.48IleLeu: 6.48 ± 0.145
1.287IleMet: 1.287 ± 0.061
3.465IleAsn: 3.465 ± 0.106
3.151IlePro: 3.151 ± 0.084
2.621IleGln: 2.621 ± 0.079
2.476IleArg: 2.476 ± 0.07
5.316IleSer: 5.316 ± 0.11
4.238IleThr: 4.238 ± 0.178
4.445IleVal: 4.445 ± 0.121
0.663IleTrp: 0.663 ± 0.042
2.361IleTyr: 2.361 ± 0.072
0.0IleXaa: 0.0 ± 0.0
Lys
6.007LysAla: 6.007 ± 0.167
0.251LysCys: 0.251 ± 0.026
4.255LysAsp: 4.255 ± 0.104
5.593LysGlu: 5.593 ± 0.138
2.148LysPhe: 2.148 ± 0.073
4.83LysGly: 4.83 ± 0.105
1.195LysHis: 1.195 ± 0.046
5.007LysIle: 5.007 ± 0.128
6.181LysLys: 6.181 ± 0.15
5.267LysLeu: 5.267 ± 0.114
1.907LysMet: 1.907 ± 0.066
4.14LysAsn: 4.14 ± 0.118
2.344LysPro: 2.344 ± 0.076
2.448LysGln: 2.448 ± 0.077
2.661LysArg: 2.661 ± 0.078
4.087LysSer: 4.087 ± 0.085
4.417LysThr: 4.417 ± 0.094
4.462LysVal: 4.462 ± 0.101
0.665LysTrp: 0.665 ± 0.043
2.41LysTyr: 2.41 ± 0.076
0.0LysXaa: 0.0 ± 0.0
Leu
7.451LeuAla: 7.451 ± 0.144
0.752LeuCys: 0.752 ± 0.047
5.764LeuAsp: 5.764 ± 0.156
6.712LeuGlu: 6.712 ± 0.155
5.056LeuPhe: 5.056 ± 0.136
6.767LeuGly: 6.767 ± 0.142
1.707LeuHis: 1.707 ± 0.066
6.478LeuIle: 6.478 ± 0.14
7.556LeuLys: 7.556 ± 0.146
9.633LeuLeu: 9.633 ± 0.192
2.342LeuMet: 2.342 ± 0.074
5.073LeuAsn: 5.073 ± 0.121
3.938LeuPro: 3.938 ± 0.097
3.309LeuGln: 3.309 ± 0.093
3.249LeuArg: 3.249 ± 0.094
7.656LeuSer: 7.656 ± 0.134
4.762LeuThr: 4.762 ± 0.124
6.071LeuVal: 6.071 ± 0.113
1.055LeuTrp: 1.055 ± 0.048
2.964LeuTyr: 2.964 ± 0.092
0.0LeuXaa: 0.0 ± 0.0
Met
2.21MetAla: 2.21 ± 0.07
0.136MetCys: 0.136 ± 0.018
1.166MetAsp: 1.166 ± 0.053
1.551MetGlu: 1.551 ± 0.061
0.848MetPhe: 0.848 ± 0.038
1.975MetGly: 1.975 ± 0.067
0.482MetHis: 0.482 ± 0.027
1.432MetIle: 1.432 ± 0.054
1.847MetLys: 1.847 ± 0.059
1.994MetLeu: 1.994 ± 0.074
0.571MetMet: 0.571 ± 0.041
1.14MetAsn: 1.14 ± 0.05
1.042MetPro: 1.042 ± 0.047
0.793MetGln: 0.793 ± 0.039
0.942MetArg: 0.942 ± 0.044
1.658MetSer: 1.658 ± 0.063
1.183MetThr: 1.183 ± 0.051
1.653MetVal: 1.653 ± 0.059
0.168MetTrp: 0.168 ± 0.018
0.626MetTyr: 0.626 ± 0.035
0.0MetXaa: 0.0 ± 0.0
Asn
4.279AsnAla: 4.279 ± 0.101
0.381AsnCys: 0.381 ± 0.029
2.416AsnAsp: 2.416 ± 0.101
2.915AsnGlu: 2.915 ± 0.081
2.512AsnPhe: 2.512 ± 0.093
3.563AsnGly: 3.563 ± 0.113
0.916AsnHis: 0.916 ± 0.039
3.823AsnIle: 3.823 ± 0.112
3.437AsnLys: 3.437 ± 0.101
4.4AsnLeu: 4.4 ± 0.126
1.11AsnMet: 1.11 ± 0.046
2.977AsnAsn: 2.977 ± 0.109
2.704AsnPro: 2.704 ± 0.073
1.956AsnGln: 1.956 ± 0.082
1.952AsnArg: 1.952 ± 0.076
3.175AsnSer: 3.175 ± 0.108
3.663AsnThr: 3.663 ± 0.111
2.646AsnVal: 2.646 ± 0.083
0.684AsnTrp: 0.684 ± 0.045
2.105AsnTyr: 2.105 ± 0.073
0.0AsnXaa: 0.0 ± 0.0
Pro
2.442ProAla: 2.442 ± 0.063
0.183ProCys: 0.183 ± 0.021
1.975ProAsp: 1.975 ± 0.073
3.136ProGlu: 3.136 ± 0.112
2.101ProPhe: 2.101 ± 0.062
2.295ProGly: 2.295 ± 0.072
0.791ProHis: 0.791 ± 0.039
2.938ProIle: 2.938 ± 0.087
2.668ProLys: 2.668 ± 0.078
3.976ProLeu: 3.976 ± 0.104
0.957ProMet: 0.957 ± 0.049
2.201ProAsn: 2.201 ± 0.073
0.923ProPro: 0.923 ± 0.056
1.406ProGln: 1.406 ± 0.056
0.989ProArg: 0.989 ± 0.042
2.591ProSer: 2.591 ± 0.084
2.18ProThr: 2.18 ± 0.067
2.453ProVal: 2.453 ± 0.077
0.371ProTrp: 0.371 ± 0.031
1.504ProTyr: 1.504 ± 0.059
0.0ProXaa: 0.0 ± 0.0
Gln
2.638GlnAla: 2.638 ± 0.077
0.194GlnCys: 0.194 ± 0.024
2.041GlnAsp: 2.041 ± 0.07
2.651GlnGlu: 2.651 ± 0.084
1.558GlnPhe: 1.558 ± 0.069
2.418GlnGly: 2.418 ± 0.068
0.652GlnHis: 0.652 ± 0.037
2.649GlnIle: 2.649 ± 0.088
2.909GlnLys: 2.909 ± 0.085
3.908GlnLeu: 3.908 ± 0.106
1.048GlnMet: 1.048 ± 0.046
1.875GlnAsn: 1.875 ± 0.084
1.063GlnPro: 1.063 ± 0.049
1.443GlnGln: 1.443 ± 0.07
1.415GlnArg: 1.415 ± 0.056
2.267GlnSer: 2.267 ± 0.077
2.001GlnThr: 2.001 ± 0.093
2.09GlnVal: 2.09 ± 0.071
0.558GlnTrp: 0.558 ± 0.035
1.464GlnTyr: 1.464 ± 0.063
0.0GlnXaa: 0.0 ± 0.0
Arg
2.497ArgAla: 2.497 ± 0.082
0.183ArgCys: 0.183 ± 0.019
1.79ArgAsp: 1.79 ± 0.06
2.146ArgGlu: 2.146 ± 0.073
2.009ArgPhe: 2.009 ± 0.067
2.207ArgGly: 2.207 ± 0.067
0.68ArgHis: 0.68 ± 0.036
2.668ArgIle: 2.668 ± 0.078
2.303ArgLys: 2.303 ± 0.074
3.401ArgLeu: 3.401 ± 0.095
0.88ArgMet: 0.88 ± 0.047
1.745ArgAsn: 1.745 ± 0.067
1.24ArgPro: 1.24 ± 0.05
1.055ArgGln: 1.055 ± 0.048
1.287ArgArg: 1.287 ± 0.068
2.116ArgSer: 2.116 ± 0.07
1.852ArgThr: 1.852 ± 0.064
2.201ArgVal: 2.201 ± 0.068
0.377ArgTrp: 0.377 ± 0.026
1.483ArgTyr: 1.483 ± 0.062
0.0ArgXaa: 0.0 ± 0.0
Ser
4.696SerAla: 4.696 ± 0.114
0.599SerCys: 0.599 ± 0.048
3.529SerAsp: 3.529 ± 0.106
3.895SerGlu: 3.895 ± 0.087
3.942SerPhe: 3.942 ± 0.09
5.267SerGly: 5.267 ± 0.158
1.325SerHis: 1.325 ± 0.062
4.805SerIle: 4.805 ± 0.118
4.402SerLys: 4.402 ± 0.102
7.304SerLeu: 7.304 ± 0.15
1.451SerMet: 1.451 ± 0.06
3.222SerAsn: 3.222 ± 0.119
2.455SerPro: 2.455 ± 0.078
2.399SerGln: 2.399 ± 0.074
2.248SerArg: 2.248 ± 0.071
4.127SerSer: 4.127 ± 0.146
3.128SerThr: 3.128 ± 0.126
4.27SerVal: 4.27 ± 0.097
0.763SerTrp: 0.763 ± 0.041
2.657SerTyr: 2.657 ± 0.08
0.0SerXaa: 0.0 ± 0.0
Thr
5.154ThrAla: 5.154 ± 0.149
0.3ThrCys: 0.3 ± 0.038
3.232ThrAsp: 3.232 ± 0.14
2.896ThrGlu: 2.896 ± 0.086
2.725ThrPhe: 2.725 ± 0.084
3.729ThrGly: 3.729 ± 0.123
1.242ThrHis: 1.242 ± 0.062
4.253ThrIle: 4.253 ± 0.157
3.407ThrLys: 3.407 ± 0.096
5.783ThrLeu: 5.783 ± 0.117
0.901ThrMet: 0.901 ± 0.046
2.672ThrAsn: 2.672 ± 0.106
3.03ThrPro: 3.03 ± 0.093
2.254ThrGln: 2.254 ± 0.074
1.622ThrArg: 1.622 ± 0.071
3.543ThrSer: 3.543 ± 0.127
3.379ThrThr: 3.379 ± 0.133
3.682ThrVal: 3.682 ± 0.134
0.441ThrTrp: 0.441 ± 0.036
2.299ThrTyr: 2.299 ± 0.098
0.0ThrXaa: 0.0 ± 0.0
Val
5.531ValAla: 5.531 ± 0.138
0.59ValCys: 0.59 ± 0.045
3.009ValAsp: 3.009 ± 0.087
2.938ValGlu: 2.938 ± 0.096
3.697ValPhe: 3.697 ± 0.098
4.364ValGly: 4.364 ± 0.104
1.187ValHis: 1.187 ± 0.058
4.455ValIle: 4.455 ± 0.12
3.923ValLys: 3.923 ± 0.114
7.019ValLeu: 7.019 ± 0.131
1.479ValMet: 1.479 ± 0.066
3.158ValAsn: 3.158 ± 0.093
2.425ValPro: 2.425 ± 0.079
2.129ValGln: 2.129 ± 0.077
2.022ValArg: 2.022 ± 0.064
5.216ValSer: 5.216 ± 0.129
3.473ValThr: 3.473 ± 0.129
4.98ValVal: 4.98 ± 0.119
0.563ValTrp: 0.563 ± 0.034
2.465ValTyr: 2.465 ± 0.078
0.0ValXaa: 0.0 ± 0.0
Trp
0.727TrpAla: 0.727 ± 0.041
0.094TrpCys: 0.094 ± 0.014
0.607TrpAsp: 0.607 ± 0.039
0.699TrpGlu: 0.699 ± 0.041
0.528TrpPhe: 0.528 ± 0.033
0.776TrpGly: 0.776 ± 0.041
0.26TrpHis: 0.26 ± 0.025
0.588TrpIle: 0.588 ± 0.036
0.714TrpLys: 0.714 ± 0.037
1.016TrpLeu: 1.016 ± 0.045
0.33TrpMet: 0.33 ± 0.027
0.616TrpAsn: 0.616 ± 0.036
0.324TrpPro: 0.324 ± 0.031
0.454TrpGln: 0.454 ± 0.04
0.475TrpArg: 0.475 ± 0.035
0.727TrpSer: 0.727 ± 0.045
0.571TrpThr: 0.571 ± 0.033
0.701TrpVal: 0.701 ± 0.038
0.17TrpTrp: 0.17 ± 0.019
0.386TrpTyr: 0.386 ± 0.027
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.621TyrAla: 2.621 ± 0.087
0.324TyrCys: 0.324 ± 0.025
1.954TyrAsp: 1.954 ± 0.077
2.163TyrGlu: 2.163 ± 0.07
2.32TyrPhe: 2.32 ± 0.083
2.747TyrGly: 2.747 ± 0.088
0.82TyrHis: 0.82 ± 0.049
2.25TyrIle: 2.25 ± 0.071
2.582TyrLys: 2.582 ± 0.075
3.733TyrLeu: 3.733 ± 0.098
0.773TyrMet: 0.773 ± 0.041
1.941TyrAsn: 1.941 ± 0.073
1.562TyrPro: 1.562 ± 0.065
1.504TyrGln: 1.504 ± 0.068
1.511TyrArg: 1.511 ± 0.064
2.229TyrSer: 2.229 ± 0.076
2.478TyrThr: 2.478 ± 0.097
1.943TyrVal: 1.943 ± 0.062
0.428TyrTrp: 0.428 ± 0.034
1.457TyrTyr: 1.457 ± 0.066
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1382 proteins (469315 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski