Isosphaera pallida (strain ATCC 43644 / DSM 9630 / IS1B)

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Isosphaerales; Isosphaeraceae; Isosphaera; Isosphaera pallida

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3721 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E8QXQ4|E8QXQ4_ISOPI Aspartate/glutamate/uridylate kinase OS=Isosphaera pallida (strain ATCC 43644 / DSM 9630 / IS1B) OX=575540 GN=Isop_3534 PE=4 SV=1
MM1 pKa = 6.73TTNNHH6 pKa = 6.7PIEE9 pKa = 4.75PDD11 pKa = 3.37GYY13 pKa = 11.17DD14 pKa = 2.86RR15 pKa = 11.84VAPGFACPSCGEE27 pKa = 4.33DD28 pKa = 5.53DD29 pKa = 3.65IDD31 pKa = 4.25SLVWIDD37 pKa = 4.25DD38 pKa = 3.74DD39 pKa = 5.25KK40 pKa = 11.8VRR42 pKa = 11.84CDD44 pKa = 3.23SCGTVYY50 pKa = 10.92DD51 pKa = 4.48PNQPDD56 pKa = 3.34AEE58 pKa = 4.21AA59 pKa = 4.81

Molecular weight: 6.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E8QXH3|E8QXH3_ISOPI DUF5664 domain-containing protein OS=Isosphaera pallida (strain ATCC 43644 / DSM 9630 / IS1B) OX=575540 GN=Isop_2447 PE=4 SV=1
MM1 pKa = 7.76LILKK5 pKa = 7.96EE6 pKa = 4.1IKK8 pKa = 9.78PRR10 pKa = 11.84APSCSPGEE18 pKa = 3.98RR19 pKa = 11.84KK20 pKa = 10.16VGGNRR25 pKa = 11.84RR26 pKa = 11.84PRR28 pKa = 11.84SWTICPVCLGIFPLPHH44 pKa = 6.15LRR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84FCSYY52 pKa = 9.16PCKK55 pKa = 10.05VTAQATGRR63 pKa = 11.84QTVRR67 pKa = 11.84RR68 pKa = 11.84TIAKK72 pKa = 9.96ARR74 pKa = 11.84TAQSLLRR81 pKa = 11.84YY82 pKa = 8.69HH83 pKa = 6.15VQAGNIVRR91 pKa = 11.84PDD93 pKa = 3.11ACEE96 pKa = 3.59EE97 pKa = 4.06CGATDD102 pKa = 3.54RR103 pKa = 11.84RR104 pKa = 11.84IEE106 pKa = 4.13AAHH109 pKa = 6.15FNYY112 pKa = 10.32DD113 pKa = 2.89EE114 pKa = 4.5PLRR117 pKa = 11.84VRR119 pKa = 11.84WLCVPCHH126 pKa = 5.95RR127 pKa = 11.84RR128 pKa = 11.84WDD130 pKa = 3.6KK131 pKa = 11.51SEE133 pKa = 4.3PKK135 pKa = 9.63HH136 pKa = 5.24ATVIIQRR143 pKa = 11.84RR144 pKa = 11.84EE145 pKa = 3.62KK146 pKa = 9.37FTGRR150 pKa = 11.84KK151 pKa = 8.77AEE153 pKa = 4.26RR154 pKa = 11.84GNGHH158 pKa = 6.66AA159 pKa = 5.26

Molecular weight: 18.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3721

0

3721

1549566

30

4765

416.4

45.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.884 ± 0.051

0.85 ± 0.013

5.81 ± 0.036

5.869 ± 0.036

3.341 ± 0.021

8.047 ± 0.054

2.186 ± 0.023

4.332 ± 0.028

2.428 ± 0.033

10.843 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.686 ± 0.018

2.783 ± 0.04

7.109 ± 0.044

3.42 ± 0.027

8.453 ± 0.043

5.647 ± 0.035

5.555 ± 0.033

7.285 ± 0.036

1.681 ± 0.024

1.793 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski