Amino acid dipepetide frequency for Mediterranea sp. An20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.645AlaAla: 6.645 ± 0.103
1.297AlaCys: 1.297 ± 0.037
5.023AlaAsp: 5.023 ± 0.055
5.198AlaGlu: 5.198 ± 0.086
3.279AlaPhe: 3.279 ± 0.061
5.658AlaGly: 5.658 ± 0.077
1.403AlaHis: 1.403 ± 0.032
4.334AlaIle: 4.334 ± 0.074
3.567AlaLys: 3.567 ± 0.064
7.705AlaLeu: 7.705 ± 0.106
1.997AlaMet: 1.997 ± 0.039
2.8AlaAsn: 2.8 ± 0.052
2.467AlaPro: 2.467 ± 0.046
3.076AlaGln: 3.076 ± 0.052
4.141AlaArg: 4.141 ± 0.071
4.263AlaSer: 4.263 ± 0.073
3.881AlaThr: 3.881 ± 0.064
5.427AlaVal: 5.427 ± 0.081
0.972AlaTrp: 0.972 ± 0.036
3.164AlaTyr: 3.164 ± 0.059
0.0AlaXaa: 0.0 ± 0.0
Cys
0.937CysAla: 0.937 ± 0.03
0.265CysCys: 0.265 ± 0.016
0.684CysAsp: 0.684 ± 0.027
0.683CysGlu: 0.683 ± 0.024
0.629CysPhe: 0.629 ± 0.026
1.25CysGly: 1.25 ± 0.032
0.37CysHis: 0.37 ± 0.02
0.948CysIle: 0.948 ± 0.032
0.634CysLys: 0.634 ± 0.023
1.522CysLeu: 1.522 ± 0.038
0.359CysMet: 0.359 ± 0.018
0.542CysAsn: 0.542 ± 0.022
0.644CysPro: 0.644 ± 0.025
0.432CysGln: 0.432 ± 0.018
0.898CysArg: 0.898 ± 0.029
0.779CysSer: 0.779 ± 0.028
0.793CysThr: 0.793 ± 0.028
0.829CysVal: 0.829 ± 0.029
0.189CysTrp: 0.189 ± 0.014
0.598CysTyr: 0.598 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
4.178AspAla: 4.178 ± 0.061
0.737AspCys: 0.737 ± 0.026
2.85AspAsp: 2.85 ± 0.057
4.224AspGlu: 4.224 ± 0.066
3.032AspPhe: 3.032 ± 0.051
4.274AspGly: 4.274 ± 0.067
0.844AspHis: 0.844 ± 0.03
3.948AspIle: 3.948 ± 0.062
3.504AspLys: 3.504 ± 0.067
4.984AspLeu: 4.984 ± 0.055
1.679AspMet: 1.679 ± 0.033
2.589AspAsn: 2.589 ± 0.05
1.831AspPro: 1.831 ± 0.045
1.135AspGln: 1.135 ± 0.031
3.147AspArg: 3.147 ± 0.057
3.04AspSer: 3.04 ± 0.061
3.139AspThr: 3.139 ± 0.062
3.602AspVal: 3.602 ± 0.065
0.905AspTrp: 0.905 ± 0.031
3.096AspTyr: 3.096 ± 0.061
0.0AspXaa: 0.0 ± 0.0
Glu
5.468GluAla: 5.468 ± 0.071
0.718GluCys: 0.718 ± 0.026
3.426GluAsp: 3.426 ± 0.061
5.413GluGlu: 5.413 ± 0.1
2.263GluPhe: 2.263 ± 0.051
4.343GluGly: 4.343 ± 0.071
1.456GluHis: 1.456 ± 0.039
4.053GluIle: 4.053 ± 0.067
4.654GluLys: 4.654 ± 0.077
6.165GluLeu: 6.165 ± 0.073
2.181GluMet: 2.181 ± 0.042
3.097GluAsn: 3.097 ± 0.058
1.894GluPro: 1.894 ± 0.043
3.043GluGln: 3.043 ± 0.063
3.922GluArg: 3.922 ± 0.078
3.0GluSer: 3.0 ± 0.049
3.53GluThr: 3.53 ± 0.06
4.481GluVal: 4.481 ± 0.059
0.853GluTrp: 0.853 ± 0.03
2.69GluTyr: 2.69 ± 0.05
0.0GluXaa: 0.0 ± 0.0
Phe
3.112PheAla: 3.112 ± 0.06
0.804PheCys: 0.804 ± 0.025
2.737PheAsp: 2.737 ± 0.05
2.263PheGlu: 2.263 ± 0.045
2.199PhePhe: 2.199 ± 0.052
3.143PheGly: 3.143 ± 0.055
0.964PheHis: 0.964 ± 0.029
2.818PheIle: 2.818 ± 0.057
1.957PheLys: 1.957 ± 0.05
4.191PheLeu: 4.191 ± 0.073
1.101PheMet: 1.101 ± 0.035
1.989PheAsn: 1.989 ± 0.049
1.698PhePro: 1.698 ± 0.04
1.276PheGln: 1.276 ± 0.039
2.584PheArg: 2.584 ± 0.049
3.103PheSer: 3.103 ± 0.061
2.527PheThr: 2.527 ± 0.053
2.87PheVal: 2.87 ± 0.052
0.542PheTrp: 0.542 ± 0.019
2.001PheTyr: 2.001 ± 0.045
0.0PheXaa: 0.0 ± 0.0
Gly
4.416GlyAla: 4.416 ± 0.073
1.051GlyCys: 1.051 ± 0.033
3.49GlyAsp: 3.49 ± 0.061
4.26GlyGlu: 4.26 ± 0.063
3.079GlyPhe: 3.079 ± 0.058
4.895GlyGly: 4.895 ± 0.091
1.502GlyHis: 1.502 ± 0.039
5.161GlyIle: 5.161 ± 0.077
5.017GlyLys: 5.017 ± 0.076
6.062GlyLeu: 6.062 ± 0.083
2.218GlyMet: 2.218 ± 0.046
3.383GlyAsn: 3.383 ± 0.06
1.369GlyPro: 1.369 ± 0.043
2.458GlyGln: 2.458 ± 0.045
3.7GlyArg: 3.7 ± 0.059
3.78GlySer: 3.78 ± 0.072
4.181GlyThr: 4.181 ± 0.059
4.727GlyVal: 4.727 ± 0.078
1.033GlyTrp: 1.033 ± 0.031
3.306GlyTyr: 3.306 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
1.478HisAla: 1.478 ± 0.037
0.36HisCys: 0.36 ± 0.02
1.055HisAsp: 1.055 ± 0.033
1.136HisGlu: 1.136 ± 0.036
1.115HisPhe: 1.115 ± 0.034
1.377HisGly: 1.377 ± 0.04
0.505HisHis: 0.505 ± 0.023
1.588HisIle: 1.588 ± 0.039
0.983HisLys: 0.983 ± 0.033
2.05HisLeu: 2.05 ± 0.044
0.367HisMet: 0.367 ± 0.02
0.863HisAsn: 0.863 ± 0.028
1.313HisPro: 1.313 ± 0.038
0.648HisGln: 0.648 ± 0.021
1.271HisArg: 1.271 ± 0.037
1.115HisSer: 1.115 ± 0.027
1.262HisThr: 1.262 ± 0.035
1.215HisVal: 1.215 ± 0.031
0.276HisTrp: 0.276 ± 0.015
0.989HisTyr: 0.989 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
4.935IleAla: 4.935 ± 0.071
0.997IleCys: 0.997 ± 0.03
3.89IleAsp: 3.89 ± 0.065
3.995IleGlu: 3.995 ± 0.063
2.425IlePhe: 2.425 ± 0.052
4.414IleGly: 4.414 ± 0.056
1.386IleHis: 1.386 ± 0.039
3.686IleIle: 3.686 ± 0.059
3.068IleLys: 3.068 ± 0.065
5.339IleLeu: 5.339 ± 0.082
1.292IleMet: 1.292 ± 0.03
2.745IleAsn: 2.745 ± 0.047
2.968IlePro: 2.968 ± 0.049
1.956IleGln: 1.956 ± 0.042
3.854IleArg: 3.854 ± 0.065
3.822IleSer: 3.822 ± 0.063
3.411IleThr: 3.411 ± 0.057
4.098IleVal: 4.098 ± 0.067
0.637IleTrp: 0.637 ± 0.027
2.455IleTyr: 2.455 ± 0.047
0.0IleXaa: 0.0 ± 0.0
Lys
4.594LysAla: 4.594 ± 0.071
0.505LysCys: 0.505 ± 0.021
3.518LysAsp: 3.518 ± 0.067
4.976LysGlu: 4.976 ± 0.078
1.807LysPhe: 1.807 ± 0.04
3.875LysGly: 3.875 ± 0.066
1.146LysHis: 1.146 ± 0.027
3.187LysIle: 3.187 ± 0.054
3.947LysLys: 3.947 ± 0.069
4.722LysLeu: 4.722 ± 0.06
1.763LysMet: 1.763 ± 0.039
2.697LysAsn: 2.697 ± 0.047
2.055LysPro: 2.055 ± 0.046
2.457LysGln: 2.457 ± 0.049
3.223LysArg: 3.223 ± 0.062
2.773LysSer: 2.773 ± 0.05
3.037LysThr: 3.037 ± 0.057
3.51LysVal: 3.51 ± 0.062
0.622LysTrp: 0.622 ± 0.023
2.444LysTyr: 2.444 ± 0.052
0.0LysXaa: 0.0 ± 0.0
Leu
7.523LeuAla: 7.523 ± 0.1
1.617LeuCys: 1.617 ± 0.037
5.0LeuAsp: 5.0 ± 0.077
5.428LeuGlu: 5.428 ± 0.073
4.53LeuPhe: 4.53 ± 0.078
5.671LeuGly: 5.671 ± 0.084
2.203LeuHis: 2.203 ± 0.045
4.995LeuIle: 4.995 ± 0.081
5.789LeuLys: 5.789 ± 0.069
10.31LeuLeu: 10.31 ± 0.142
2.647LeuMet: 2.647 ± 0.05
4.356LeuAsn: 4.356 ± 0.069
4.696LeuPro: 4.696 ± 0.082
3.936LeuGln: 3.936 ± 0.073
5.192LeuArg: 5.192 ± 0.074
6.478LeuSer: 6.478 ± 0.09
5.661LeuThr: 5.661 ± 0.07
5.579LeuVal: 5.579 ± 0.073
1.167LeuTrp: 1.167 ± 0.034
3.919LeuTyr: 3.919 ± 0.072
0.0LeuXaa: 0.0 ± 0.0
Met
2.214MetAla: 2.214 ± 0.05
0.269MetCys: 0.269 ± 0.016
1.594MetAsp: 1.594 ± 0.037
1.988MetGlu: 1.988 ± 0.047
0.945MetPhe: 0.945 ± 0.031
1.828MetGly: 1.828 ± 0.042
0.479MetHis: 0.479 ± 0.019
1.263MetIle: 1.263 ± 0.038
2.252MetLys: 2.252 ± 0.042
2.631MetLeu: 2.631 ± 0.055
0.742MetMet: 0.742 ± 0.025
1.434MetAsn: 1.434 ± 0.038
1.318MetPro: 1.318 ± 0.037
1.247MetGln: 1.247 ± 0.035
1.509MetArg: 1.509 ± 0.044
1.463MetSer: 1.463 ± 0.04
1.48MetThr: 1.48 ± 0.036
1.536MetVal: 1.536 ± 0.037
0.244MetTrp: 0.244 ± 0.016
0.837MetTyr: 0.837 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
3.331AsnAla: 3.331 ± 0.061
0.48AsnCys: 0.48 ± 0.022
2.334AsnAsp: 2.334 ± 0.052
2.794AsnGlu: 2.794 ± 0.057
1.787AsnPhe: 1.787 ± 0.042
3.49AsnGly: 3.49 ± 0.061
0.866AsnHis: 0.866 ± 0.029
3.105AsnIle: 3.105 ± 0.06
2.323AsnLys: 2.323 ± 0.049
4.18AsnLeu: 4.18 ± 0.064
1.107AsnMet: 1.107 ± 0.034
2.035AsnAsn: 2.035 ± 0.055
2.358AsnPro: 2.358 ± 0.054
1.391AsnGln: 1.391 ± 0.036
2.679AsnArg: 2.679 ± 0.054
2.223AsnSer: 2.223 ± 0.045
2.222AsnThr: 2.222 ± 0.057
2.815AsnVal: 2.815 ± 0.052
0.595AsnTrp: 0.595 ± 0.026
2.005AsnTyr: 2.005 ± 0.053
0.0AsnXaa: 0.0 ± 0.0
Pro
3.275ProAla: 3.275 ± 0.064
0.467ProCys: 0.467 ± 0.025
2.959ProAsp: 2.959 ± 0.054
3.378ProGlu: 3.378 ± 0.059
1.833ProPhe: 1.833 ± 0.045
2.654ProGly: 2.654 ± 0.058
0.904ProHis: 0.904 ± 0.03
2.088ProIle: 2.088 ± 0.041
1.658ProLys: 1.658 ± 0.038
3.785ProLeu: 3.785 ± 0.064
1.009ProMet: 1.009 ± 0.034
1.506ProAsn: 1.506 ± 0.039
0.998ProPro: 0.998 ± 0.034
1.628ProGln: 1.628 ± 0.041
1.613ProArg: 1.613 ± 0.04
2.207ProSer: 2.207 ± 0.041
1.978ProThr: 1.978 ± 0.039
3.165ProVal: 3.165 ± 0.052
0.47ProTrp: 0.47 ± 0.02
1.799ProTyr: 1.799 ± 0.037
0.0ProXaa: 0.0 ± 0.0
Gln
3.073GlnAla: 3.073 ± 0.055
0.369GlnCys: 0.369 ± 0.02
1.756GlnAsp: 1.756 ± 0.042
2.771GlnGlu: 2.771 ± 0.055
1.283GlnPhe: 1.283 ± 0.035
2.413GlnGly: 2.413 ± 0.047
0.781GlnHis: 0.781 ± 0.026
2.208GlnIle: 2.208 ± 0.046
2.234GlnLys: 2.234 ± 0.045
3.422GlnLeu: 3.422 ± 0.062
1.177GlnMet: 1.177 ± 0.033
1.473GlnAsn: 1.473 ± 0.042
1.498GlnPro: 1.498 ± 0.038
1.829GlnGln: 1.829 ± 0.047
2.074GlnArg: 2.074 ± 0.041
1.943GlnSer: 1.943 ± 0.046
2.376GlnThr: 2.376 ± 0.045
2.444GlnVal: 2.444 ± 0.048
0.548GlnTrp: 0.548 ± 0.022
1.411GlnTyr: 1.411 ± 0.042
0.0GlnXaa: 0.0 ± 0.0
Arg
3.487ArgAla: 3.487 ± 0.063
0.661ArgCys: 0.661 ± 0.027
2.649ArgAsp: 2.649 ± 0.049
3.744ArgGlu: 3.744 ± 0.068
2.588ArgPhe: 2.588 ± 0.05
2.846ArgGly: 2.846 ± 0.055
1.455ArgHis: 1.455 ± 0.04
4.016ArgIle: 4.016 ± 0.066
3.675ArgLys: 3.675 ± 0.064
5.971ArgLeu: 5.971 ± 0.078
1.852ArgMet: 1.852 ± 0.042
2.625ArgAsn: 2.625 ± 0.054
2.082ArgPro: 2.082 ± 0.045
2.691ArgGln: 2.691 ± 0.06
3.657ArgArg: 3.657 ± 0.071
2.659ArgSer: 2.659 ± 0.055
2.972ArgThr: 2.972 ± 0.053
3.162ArgVal: 3.162 ± 0.058
0.79ArgTrp: 0.79 ± 0.026
2.548ArgTyr: 2.548 ± 0.052
0.0ArgXaa: 0.0 ± 0.0
Ser
4.111SerAla: 4.111 ± 0.068
0.819SerCys: 0.819 ± 0.028
3.159SerAsp: 3.159 ± 0.057
3.207SerGlu: 3.207 ± 0.052
3.02SerPhe: 3.02 ± 0.064
4.27SerGly: 4.27 ± 0.066
1.144SerHis: 1.144 ± 0.031
3.676SerIle: 3.676 ± 0.063
2.662SerLys: 2.662 ± 0.053
5.963SerLeu: 5.963 ± 0.078
1.4SerMet: 1.4 ± 0.035
2.19SerAsn: 2.19 ± 0.048
2.298SerPro: 2.298 ± 0.043
1.799SerGln: 1.799 ± 0.041
3.016SerArg: 3.016 ± 0.056
3.3SerSer: 3.3 ± 0.074
2.904SerThr: 2.904 ± 0.058
4.094SerVal: 4.094 ± 0.068
0.769SerTrp: 0.769 ± 0.028
2.527SerTyr: 2.527 ± 0.051
0.0SerXaa: 0.0 ± 0.0
Thr
4.39ThrAla: 4.39 ± 0.066
0.643ThrCys: 0.643 ± 0.025
3.755ThrAsp: 3.755 ± 0.059
3.503ThrGlu: 3.503 ± 0.059
2.499ThrPhe: 2.499 ± 0.05
4.472ThrGly: 4.472 ± 0.071
1.09ThrHis: 1.09 ± 0.034
3.114ThrIle: 3.114 ± 0.054
2.211ThrLys: 2.211 ± 0.053
5.923ThrLeu: 5.923 ± 0.079
1.16ThrMet: 1.16 ± 0.032
2.062ThrAsn: 2.062 ± 0.048
2.923ThrPro: 2.923 ± 0.049
1.778ThrGln: 1.778 ± 0.039
2.515ThrArg: 2.515 ± 0.049
2.966ThrSer: 2.966 ± 0.055
2.906ThrThr: 2.906 ± 0.052
4.177ThrVal: 4.177 ± 0.066
0.739ThrTrp: 0.739 ± 0.029
2.336ThrTyr: 2.336 ± 0.048
0.0ThrXaa: 0.0 ± 0.0
Val
5.063ValAla: 5.063 ± 0.078
1.201ValCys: 1.201 ± 0.036
3.602ValAsp: 3.602 ± 0.058
4.327ValGlu: 4.327 ± 0.064
2.927ValPhe: 2.927 ± 0.051
4.216ValGly: 4.216 ± 0.071
1.195ValHis: 1.195 ± 0.036
3.916ValIle: 3.916 ± 0.061
3.753ValLys: 3.753 ± 0.057
6.177ValLeu: 6.177 ± 0.081
1.719ValMet: 1.719 ± 0.042
2.905ValAsn: 2.905 ± 0.054
2.755ValPro: 2.755 ± 0.051
2.175ValGln: 2.175 ± 0.044
3.75ValArg: 3.75 ± 0.072
4.342ValSer: 4.342 ± 0.07
3.713ValThr: 3.713 ± 0.055
4.663ValVal: 4.663 ± 0.079
0.798ValTrp: 0.798 ± 0.025
2.585ValTyr: 2.585 ± 0.05
0.0ValXaa: 0.0 ± 0.0
Trp
0.8TrpAla: 0.8 ± 0.028
0.173TrpCys: 0.173 ± 0.013
0.69TrpAsp: 0.69 ± 0.024
0.811TrpGlu: 0.811 ± 0.025
0.583TrpPhe: 0.583 ± 0.024
1.013TrpGly: 1.013 ± 0.035
0.318TrpHis: 0.318 ± 0.017
0.755TrpIle: 0.755 ± 0.028
0.839TrpLys: 0.839 ± 0.03
1.417TrpLeu: 1.417 ± 0.036
0.465TrpMet: 0.465 ± 0.022
0.773TrpAsn: 0.773 ± 0.03
0.316TrpPro: 0.316 ± 0.02
0.647TrpGln: 0.647 ± 0.026
0.643TrpArg: 0.643 ± 0.025
0.647TrpSer: 0.647 ± 0.027
0.678TrpThr: 0.678 ± 0.025
0.686TrpVal: 0.686 ± 0.026
0.197TrpTrp: 0.197 ± 0.015
0.499TrpTyr: 0.499 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.268TyrAla: 3.268 ± 0.066
0.553TyrCys: 0.553 ± 0.023
2.569TyrAsp: 2.569 ± 0.051
2.551TyrGlu: 2.551 ± 0.051
2.056TyrPhe: 2.056 ± 0.045
2.986TyrGly: 2.986 ± 0.059
0.955TyrHis: 0.955 ± 0.032
2.511TyrIle: 2.511 ± 0.049
2.132TyrLys: 2.132 ± 0.047
4.177TyrLeu: 4.177 ± 0.069
1.037TyrMet: 1.037 ± 0.031
2.056TyrAsn: 2.056 ± 0.044
1.89TyrPro: 1.89 ± 0.044
1.477TyrGln: 1.477 ± 0.039
2.744TyrArg: 2.744 ± 0.05
2.427TyrSer: 2.427 ± 0.053
2.511TyrThr: 2.511 ± 0.055
2.703TyrVal: 2.703 ± 0.052
0.604TyrTrp: 0.604 ± 0.023
2.059TyrTyr: 2.059 ± 0.054
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3285 proteins (1098706 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski