Fusarium poae mitovirus 1
Average proteome isoelectric point is 8.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B4ZA49|A0A1B4ZA49_9VIRU RNA-dependent RNA polymerase OS=Fusarium poae mitovirus 1 OX=1848150 PE=4 SV=1
MM1 pKa = 7.56 LRR3 pKa = 11.84 NQIKK7 pKa = 10.06 IIKK10 pKa = 8.43 RR11 pKa = 11.84 LCSIFFPRR19 pKa = 11.84 QFQPQHH25 pKa = 5.43 FVEE28 pKa = 5.68 LYY30 pKa = 10.11 KK31 pKa = 10.85 LYY33 pKa = 10.82 NSLIKK38 pKa = 9.9 FHH40 pKa = 7.08 GYY42 pKa = 9.75 GGAIKK47 pKa = 9.98 YY48 pKa = 9.35 IKK50 pKa = 10.37 LMRR53 pKa = 11.84 LHH55 pKa = 6.41 CTRR58 pKa = 11.84 YY59 pKa = 9.61 ICGNPLKK66 pKa = 9.9 TNSNGIGLTKK76 pKa = 10.51 DD77 pKa = 2.9 GWPIKK82 pKa = 10.64 LLFLKK87 pKa = 10.68 KK88 pKa = 10.56 SVDD91 pKa = 3.62 EE92 pKa = 4.72 GNFSYY97 pKa = 11.35 VLTLLMFNRR106 pKa = 11.84 SIDD109 pKa = 3.86 LPKK112 pKa = 10.65 HH113 pKa = 4.34 EE114 pKa = 5.24 VKK116 pKa = 10.73 KK117 pKa = 10.88 KK118 pKa = 8.86 MKK120 pKa = 9.84 KK121 pKa = 10.32 LSFNSITQPSTCKK134 pKa = 9.22 YY135 pKa = 8.0 TIPTGFIKK143 pKa = 10.56 EE144 pKa = 3.89 FVRR147 pKa = 11.84 INNLKK152 pKa = 9.88 ISHH155 pKa = 6.94 ANMEE159 pKa = 4.68 LNPKK163 pKa = 10.17 DD164 pKa = 4.36 FYY166 pKa = 11.35 LSNKK170 pKa = 9.71 AGPQGKK176 pKa = 9.09 SVLTAHH182 pKa = 6.8 RR183 pKa = 11.84 NWVTFTDD190 pKa = 4.23 SFIQRR195 pKa = 11.84 LNKK198 pKa = 10.09 LAVGGYY204 pKa = 10.01 AVDD207 pKa = 3.26 WLRR210 pKa = 11.84 RR211 pKa = 11.84 SQKK214 pKa = 9.48 FWQDD218 pKa = 3.02 QIDD221 pKa = 4.12 NIKK224 pKa = 10.35 SSPYY228 pKa = 9.54 HH229 pKa = 6.93 AYY231 pKa = 9.54 QGKK234 pKa = 10.02 LSVVKK239 pKa = 10.62 DD240 pKa = 3.57 PEE242 pKa = 4.32 GKK244 pKa = 9.87 FRR246 pKa = 11.84 IIAIVDD252 pKa = 3.65 YY253 pKa = 8.85 YY254 pKa = 10.73 TQLFLKK260 pKa = 10.07 KK261 pKa = 10.16 LHH263 pKa = 6.63 LEE265 pKa = 3.76 QFRR268 pKa = 11.84 LIKK271 pKa = 10.88 NLDD274 pKa = 3.38 CDD276 pKa = 3.64 KK277 pKa = 11.06 TFTQDD282 pKa = 3.38 PTHH285 pKa = 6.37 HH286 pKa = 6.93 WDD288 pKa = 3.48 YY289 pKa = 11.55 SNKK292 pKa = 9.8 EE293 pKa = 4.43 SFWSLDD299 pKa = 3.3 LSSATDD305 pKa = 3.69 RR306 pKa = 11.84 FPRR309 pKa = 11.84 ILQFRR314 pKa = 11.84 LIGEE318 pKa = 4.33 MYY320 pKa = 10.27 NYY322 pKa = 10.86 DD323 pKa = 3.8 FAKK326 pKa = 10.15 VWNEE330 pKa = 3.57 HH331 pKa = 6.05 LGSLEE336 pKa = 3.63 FSTPDD341 pKa = 3.47 GEE343 pKa = 4.59 KK344 pKa = 9.43 IQYY347 pKa = 9.86 NAGQPMGTYY356 pKa = 10.06 SSWVSFTLAHH366 pKa = 6.42 HH367 pKa = 6.93 LVVHH371 pKa = 6.25 WCAKK375 pKa = 10.35 LEE377 pKa = 4.08 GVKK380 pKa = 10.93 DD381 pKa = 3.91 FDD383 pKa = 3.96 QYY385 pKa = 11.43 IILGDD390 pKa = 4.31 DD391 pKa = 3.29 IVIKK395 pKa = 10.4 HH396 pKa = 5.6 NKK398 pKa = 7.14 IAKK401 pKa = 9.2 RR402 pKa = 11.84 YY403 pKa = 7.5 IKK405 pKa = 10.27 IMEE408 pKa = 4.32 KK409 pKa = 10.62 LGVEE413 pKa = 4.29 LSLAKK418 pKa = 9.57 THH420 pKa = 5.81 VSEE423 pKa = 4.85 DD424 pKa = 3.44 TYY426 pKa = 11.63 EE427 pKa = 3.98 FAKK430 pKa = 10.37 RR431 pKa = 11.84 WFKK434 pKa = 11.36 NSIEE438 pKa = 4.23 VTGLPTRR445 pKa = 11.84 GIIHH449 pKa = 6.31 NFKK452 pKa = 10.66 SPNIVFTILYY462 pKa = 9.92 DD463 pKa = 4.05 FYY465 pKa = 11.21 KK466 pKa = 10.45 IKK468 pKa = 11.12 NNTYY472 pKa = 10.56 LSFNSLVDD480 pKa = 3.61 SMSRR484 pKa = 11.84 LYY486 pKa = 11.23 KK487 pKa = 10.57 NFYY490 pKa = 10.49 LLEE493 pKa = 4.21 KK494 pKa = 10.01 VRR496 pKa = 11.84 KK497 pKa = 7.77 NTYY500 pKa = 9.09 KK501 pKa = 10.82 KK502 pKa = 10.28 IFIRR506 pKa = 11.84 FNRR509 pKa = 11.84 KK510 pKa = 8.78 IYY512 pKa = 11.08 NNLLDD517 pKa = 4.58 FSTVLDD523 pKa = 4.19 FSFGYY528 pKa = 10.5 EE529 pKa = 4.06 DD530 pKa = 3.58 NQKK533 pKa = 10.65 LSEE536 pKa = 4.11 LFNRR540 pKa = 11.84 KK541 pKa = 9.22 LYY543 pKa = 10.81 LSEE546 pKa = 4.42 QYY548 pKa = 10.21 TIPATMKK555 pKa = 10.21 SLRR558 pKa = 11.84 TIIGIGLKK566 pKa = 10.54 DD567 pKa = 4.39 KK568 pKa = 10.67 IVEE571 pKa = 4.26 QIKK574 pKa = 8.47 TLDD577 pKa = 3.4 NLKK580 pKa = 10.39 SHH582 pKa = 6.63 FNKK585 pKa = 9.66 MDD587 pKa = 4.06 FKK589 pKa = 10.8 TDD591 pKa = 2.98 NPMRR595 pKa = 11.84 GAFIKK600 pKa = 10.68 LHH602 pKa = 5.75 LVWPLYY608 pKa = 10.42 NGVRR612 pKa = 11.84 NEE614 pKa = 3.73 IQDD617 pKa = 3.63 LYY619 pKa = 11.32 KK620 pKa = 10.7 SYY622 pKa = 11.22 NSKK625 pKa = 9.89 IDD627 pKa = 3.62 KK628 pKa = 10.75 NNMSIYY634 pKa = 9.79 EE635 pKa = 4.16 YY636 pKa = 10.14 VSEE639 pKa = 4.45 VRR641 pKa = 11.84 IIDD644 pKa = 3.25 IQGIYY649 pKa = 9.75 EE650 pKa = 4.13 KK651 pKa = 10.5 QRR653 pKa = 11.84 QKK655 pKa = 11.18 YY656 pKa = 7.53 ATLLTIGQTMNRR668 pKa = 11.84 GFSQVNQYY676 pKa = 8.46 MKK678 pKa = 10.93 SNEE681 pKa = 4.08 NNPLMGPNLYY691 pKa = 10.47 FINILANAHH700 pKa = 6.85 FDD702 pKa = 3.73 MKK704 pKa = 10.5 WNSEE708 pKa = 3.8 KK709 pKa = 10.9 KK710 pKa = 10.35 KK711 pKa = 10.82 FNEE714 pKa = 3.96 GWNPASLIGNLTT726 pKa = 3.32
Molecular weight: 85.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.86
IPC2_protein 9.077
IPC_protein 8.931
Toseland 9.663
ProMoST 9.414
Dawson 9.926
Bjellqvist 9.604
Wikipedia 10.101
Rodwell 10.321
Grimsley 10.014
Solomon 9.94
Lehninger 9.897
Nozaki 9.692
DTASelect 9.589
Thurlkill 9.765
EMBOSS 10.087
Sillero 9.838
Patrickios 5.13
IPC_peptide 9.926
IPC2_peptide 8.112
IPC2.peptide.svr19 8.105
Protein with the highest isoelectric point:
>tr|A0A1B4ZA49|A0A1B4ZA49_9VIRU RNA-dependent RNA polymerase OS=Fusarium poae mitovirus 1 OX=1848150 PE=4 SV=1
MM1 pKa = 7.56 LRR3 pKa = 11.84 NQIKK7 pKa = 10.06 IIKK10 pKa = 8.43 RR11 pKa = 11.84 LCSIFFPRR19 pKa = 11.84 QFQPQHH25 pKa = 5.43 FVEE28 pKa = 5.68 LYY30 pKa = 10.11 KK31 pKa = 10.85 LYY33 pKa = 10.82 NSLIKK38 pKa = 9.9 FHH40 pKa = 7.08 GYY42 pKa = 9.75 GGAIKK47 pKa = 9.98 YY48 pKa = 9.35 IKK50 pKa = 10.37 LMRR53 pKa = 11.84 LHH55 pKa = 6.41 CTRR58 pKa = 11.84 YY59 pKa = 9.61 ICGNPLKK66 pKa = 9.9 TNSNGIGLTKK76 pKa = 10.51 DD77 pKa = 2.9 GWPIKK82 pKa = 10.64 LLFLKK87 pKa = 10.68 KK88 pKa = 10.56 SVDD91 pKa = 3.62 EE92 pKa = 4.72 GNFSYY97 pKa = 11.35 VLTLLMFNRR106 pKa = 11.84 SIDD109 pKa = 3.86 LPKK112 pKa = 10.65 HH113 pKa = 4.34 EE114 pKa = 5.24 VKK116 pKa = 10.73 KK117 pKa = 10.88 KK118 pKa = 8.86 MKK120 pKa = 9.84 KK121 pKa = 10.32 LSFNSITQPSTCKK134 pKa = 9.22 YY135 pKa = 8.0 TIPTGFIKK143 pKa = 10.56 EE144 pKa = 3.89 FVRR147 pKa = 11.84 INNLKK152 pKa = 9.88 ISHH155 pKa = 6.94 ANMEE159 pKa = 4.68 LNPKK163 pKa = 10.17 DD164 pKa = 4.36 FYY166 pKa = 11.35 LSNKK170 pKa = 9.71 AGPQGKK176 pKa = 9.09 SVLTAHH182 pKa = 6.8 RR183 pKa = 11.84 NWVTFTDD190 pKa = 4.23 SFIQRR195 pKa = 11.84 LNKK198 pKa = 10.09 LAVGGYY204 pKa = 10.01 AVDD207 pKa = 3.26 WLRR210 pKa = 11.84 RR211 pKa = 11.84 SQKK214 pKa = 9.48 FWQDD218 pKa = 3.02 QIDD221 pKa = 4.12 NIKK224 pKa = 10.35 SSPYY228 pKa = 9.54 HH229 pKa = 6.93 AYY231 pKa = 9.54 QGKK234 pKa = 10.02 LSVVKK239 pKa = 10.62 DD240 pKa = 3.57 PEE242 pKa = 4.32 GKK244 pKa = 9.87 FRR246 pKa = 11.84 IIAIVDD252 pKa = 3.65 YY253 pKa = 8.85 YY254 pKa = 10.73 TQLFLKK260 pKa = 10.07 KK261 pKa = 10.16 LHH263 pKa = 6.63 LEE265 pKa = 3.76 QFRR268 pKa = 11.84 LIKK271 pKa = 10.88 NLDD274 pKa = 3.38 CDD276 pKa = 3.64 KK277 pKa = 11.06 TFTQDD282 pKa = 3.38 PTHH285 pKa = 6.37 HH286 pKa = 6.93 WDD288 pKa = 3.48 YY289 pKa = 11.55 SNKK292 pKa = 9.8 EE293 pKa = 4.43 SFWSLDD299 pKa = 3.3 LSSATDD305 pKa = 3.69 RR306 pKa = 11.84 FPRR309 pKa = 11.84 ILQFRR314 pKa = 11.84 LIGEE318 pKa = 4.33 MYY320 pKa = 10.27 NYY322 pKa = 10.86 DD323 pKa = 3.8 FAKK326 pKa = 10.15 VWNEE330 pKa = 3.57 HH331 pKa = 6.05 LGSLEE336 pKa = 3.63 FSTPDD341 pKa = 3.47 GEE343 pKa = 4.59 KK344 pKa = 9.43 IQYY347 pKa = 9.86 NAGQPMGTYY356 pKa = 10.06 SSWVSFTLAHH366 pKa = 6.42 HH367 pKa = 6.93 LVVHH371 pKa = 6.25 WCAKK375 pKa = 10.35 LEE377 pKa = 4.08 GVKK380 pKa = 10.93 DD381 pKa = 3.91 FDD383 pKa = 3.96 QYY385 pKa = 11.43 IILGDD390 pKa = 4.31 DD391 pKa = 3.29 IVIKK395 pKa = 10.4 HH396 pKa = 5.6 NKK398 pKa = 7.14 IAKK401 pKa = 9.2 RR402 pKa = 11.84 YY403 pKa = 7.5 IKK405 pKa = 10.27 IMEE408 pKa = 4.32 KK409 pKa = 10.62 LGVEE413 pKa = 4.29 LSLAKK418 pKa = 9.57 THH420 pKa = 5.81 VSEE423 pKa = 4.85 DD424 pKa = 3.44 TYY426 pKa = 11.63 EE427 pKa = 3.98 FAKK430 pKa = 10.37 RR431 pKa = 11.84 WFKK434 pKa = 11.36 NSIEE438 pKa = 4.23 VTGLPTRR445 pKa = 11.84 GIIHH449 pKa = 6.31 NFKK452 pKa = 10.66 SPNIVFTILYY462 pKa = 9.92 DD463 pKa = 4.05 FYY465 pKa = 11.21 KK466 pKa = 10.45 IKK468 pKa = 11.12 NNTYY472 pKa = 10.56 LSFNSLVDD480 pKa = 3.61 SMSRR484 pKa = 11.84 LYY486 pKa = 11.23 KK487 pKa = 10.57 NFYY490 pKa = 10.49 LLEE493 pKa = 4.21 KK494 pKa = 10.01 VRR496 pKa = 11.84 KK497 pKa = 7.77 NTYY500 pKa = 9.09 KK501 pKa = 10.82 KK502 pKa = 10.28 IFIRR506 pKa = 11.84 FNRR509 pKa = 11.84 KK510 pKa = 8.78 IYY512 pKa = 11.08 NNLLDD517 pKa = 4.58 FSTVLDD523 pKa = 4.19 FSFGYY528 pKa = 10.5 EE529 pKa = 4.06 DD530 pKa = 3.58 NQKK533 pKa = 10.65 LSEE536 pKa = 4.11 LFNRR540 pKa = 11.84 KK541 pKa = 9.22 LYY543 pKa = 10.81 LSEE546 pKa = 4.42 QYY548 pKa = 10.21 TIPATMKK555 pKa = 10.21 SLRR558 pKa = 11.84 TIIGIGLKK566 pKa = 10.54 DD567 pKa = 4.39 KK568 pKa = 10.67 IVEE571 pKa = 4.26 QIKK574 pKa = 8.47 TLDD577 pKa = 3.4 NLKK580 pKa = 10.39 SHH582 pKa = 6.63 FNKK585 pKa = 9.66 MDD587 pKa = 4.06 FKK589 pKa = 10.8 TDD591 pKa = 2.98 NPMRR595 pKa = 11.84 GAFIKK600 pKa = 10.68 LHH602 pKa = 5.75 LVWPLYY608 pKa = 10.42 NGVRR612 pKa = 11.84 NEE614 pKa = 3.73 IQDD617 pKa = 3.63 LYY619 pKa = 11.32 KK620 pKa = 10.7 SYY622 pKa = 11.22 NSKK625 pKa = 9.89 IDD627 pKa = 3.62 KK628 pKa = 10.75 NNMSIYY634 pKa = 9.79 EE635 pKa = 4.16 YY636 pKa = 10.14 VSEE639 pKa = 4.45 VRR641 pKa = 11.84 IIDD644 pKa = 3.25 IQGIYY649 pKa = 9.75 EE650 pKa = 4.13 KK651 pKa = 10.5 QRR653 pKa = 11.84 QKK655 pKa = 11.18 YY656 pKa = 7.53 ATLLTIGQTMNRR668 pKa = 11.84 GFSQVNQYY676 pKa = 8.46 MKK678 pKa = 10.93 SNEE681 pKa = 4.08 NNPLMGPNLYY691 pKa = 10.47 FINILANAHH700 pKa = 6.85 FDD702 pKa = 3.73 MKK704 pKa = 10.5 WNSEE708 pKa = 3.8 KK709 pKa = 10.9 KK710 pKa = 10.35 KK711 pKa = 10.82 FNEE714 pKa = 3.96 GWNPASLIGNLTT726 pKa = 3.32
Molecular weight: 85.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.86
IPC2_protein 9.077
IPC_protein 8.931
Toseland 9.663
ProMoST 9.414
Dawson 9.926
Bjellqvist 9.604
Wikipedia 10.101
Rodwell 10.321
Grimsley 10.014
Solomon 9.94
Lehninger 9.897
Nozaki 9.692
DTASelect 9.589
Thurlkill 9.765
EMBOSS 10.087
Sillero 9.838
Patrickios 5.13
IPC_peptide 9.926
IPC2_peptide 8.112
IPC2.peptide.svr19 8.105
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
726
726
726
726.0
85.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.03 ± 0.0
0.826 ± 0.0
4.959 ± 0.0
4.132 ± 0.0
6.336 ± 0.0
4.959 ± 0.0
2.755 ± 0.0
8.264 ± 0.0
10.331 ± 0.0
10.055 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.342 ± 0.0
7.576 ± 0.0
3.168 ± 0.0
3.857 ± 0.0
4.132 ± 0.0
6.749 ± 0.0
4.959 ± 0.0
4.27 ± 0.0
1.791 ± 0.0
5.51 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here