Bombus vosnesenskii

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; Apinae; Bombini;

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19375 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J3L2N8|A0A6J3L2N8_9HYME facilitated trehalose transporter Tret1-like isoform X1 OS=Bombus vosnesenskii OX=207650 GN=LOC117238603 PE=4 SV=1
MM1 pKa = 7.11VVLSNFLAPQEE12 pKa = 4.57GIRR15 pKa = 11.84HH16 pKa = 5.84EE17 pKa = 4.33EE18 pKa = 4.11LNTTVYY24 pKa = 10.75INDD27 pKa = 3.7RR28 pKa = 11.84EE29 pKa = 4.38VGKK32 pKa = 8.77GTLYY36 pKa = 9.3ITEE39 pKa = 4.71SLLSWVNYY47 pKa = 7.76DD48 pKa = 3.57TQQGFSLEE56 pKa = 4.16YY57 pKa = 10.02PHH59 pKa = 7.27ISLHH63 pKa = 6.32AISRR67 pKa = 11.84DD68 pKa = 3.61EE69 pKa = 3.96QAHH72 pKa = 6.04PRR74 pKa = 11.84QCLYY78 pKa = 10.88IMVDD82 pKa = 3.51AKK84 pKa = 11.27VDD86 pKa = 3.97LPDD89 pKa = 3.63VPLPPASDD97 pKa = 3.43SGSDD101 pKa = 4.18NEE103 pKa = 5.62FEE105 pKa = 5.5DD106 pKa = 4.37ADD108 pKa = 4.12TPITEE113 pKa = 4.27MRR115 pKa = 11.84FAPDD119 pKa = 3.05NTNNLEE125 pKa = 4.18TMFQAMNQCQALHH138 pKa = 7.44PDD140 pKa = 3.88PQDD143 pKa = 3.37SFSDD147 pKa = 3.74AEE149 pKa = 3.83EE150 pKa = 5.32DD151 pKa = 3.31IYY153 pKa = 11.51EE154 pKa = 4.54DD155 pKa = 4.26AEE157 pKa = 4.13EE158 pKa = 5.57DD159 pKa = 4.31DD160 pKa = 4.38FEE162 pKa = 5.13QYY164 pKa = 11.16DD165 pKa = 3.98VGAGDD170 pKa = 4.51APYY173 pKa = 10.34ILPTEE178 pKa = 4.35QIGTSHH184 pKa = 7.01NGTEE188 pKa = 4.35VDD190 pKa = 3.47DD191 pKa = 5.22AMDD194 pKa = 3.64IEE196 pKa = 4.94AGQFEE201 pKa = 4.76DD202 pKa = 5.49AEE204 pKa = 4.39EE205 pKa = 4.16DD206 pKa = 3.59LL207 pKa = 5.34

Molecular weight: 23.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J3KZR8|A0A6J3KZR8_9HYME NGFI-A-binding protein homolog isoform X1 OS=Bombus vosnesenskii OX=207650 GN=LOC117238230 PE=3 SV=1
MM1 pKa = 7.44SAHH4 pKa = 6.1KK5 pKa = 9.54TFIIKK10 pKa = 10.29RR11 pKa = 11.84KK12 pKa = 8.65LAKK15 pKa = 9.89KK16 pKa = 10.19LKK18 pKa = 8.64QNRR21 pKa = 11.84PIPQWVRR28 pKa = 11.84MRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.47RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.1WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.89LKK50 pKa = 10.55LL51 pKa = 3.39

Molecular weight: 6.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10990

8385

19375

14450676

34

19554

745.8

83.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.093 ± 0.018

1.856 ± 0.02

5.373 ± 0.012

7.111 ± 0.033

3.288 ± 0.013

5.474 ± 0.02

2.493 ± 0.009

5.586 ± 0.015

6.368 ± 0.025

8.841 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.214 ± 0.007

5.112 ± 0.017

5.233 ± 0.024

4.688 ± 0.021

5.618 ± 0.017

8.513 ± 0.022

6.195 ± 0.019

5.956 ± 0.015

1.037 ± 0.006

2.95 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski