Amino acid dipepetide frequency for Klebsiella phage KP32

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.466AlaAla: 8.466 ± 0.896
0.627AlaCys: 0.627 ± 0.239
6.193AlaAsp: 6.193 ± 0.771
4.86AlaGlu: 4.86 ± 0.662
3.136AlaPhe: 3.136 ± 0.499
7.29AlaGly: 7.29 ± 1.164
1.254AlaHis: 1.254 ± 0.249
4.233AlaIle: 4.233 ± 0.468
5.879AlaLys: 5.879 ± 0.731
7.604AlaLeu: 7.604 ± 1.045
2.9AlaMet: 2.9 ± 0.574
4.782AlaAsn: 4.782 ± 0.442
3.371AlaPro: 3.371 ± 0.519
3.527AlaGln: 3.527 ± 0.594
5.095AlaArg: 5.095 ± 0.725
5.017AlaSer: 5.017 ± 0.662
4.703AlaThr: 4.703 ± 0.529
5.801AlaVal: 5.801 ± 0.635
1.097AlaTrp: 1.097 ± 0.309
3.057AlaTyr: 3.057 ± 0.489
0.0AlaXaa: 0.0 ± 0.0
Cys
0.705CysAla: 0.705 ± 0.234
0.157CysCys: 0.157 ± 0.116
0.47CysAsp: 0.47 ± 0.267
0.549CysGlu: 0.549 ± 0.209
0.627CysPhe: 0.627 ± 0.228
0.705CysGly: 0.705 ± 0.187
0.157CysHis: 0.157 ± 0.093
0.627CysIle: 0.627 ± 0.247
0.47CysLys: 0.47 ± 0.161
0.941CysLeu: 0.941 ± 0.235
0.0CysMet: 0.0 ± 0.0
0.157CysAsn: 0.157 ± 0.093
0.47CysPro: 0.47 ± 0.152
0.47CysGln: 0.47 ± 0.242
0.784CysArg: 0.784 ± 0.26
0.627CysSer: 0.627 ± 0.306
0.47CysThr: 0.47 ± 0.274
0.941CysVal: 0.941 ± 0.27
0.235CysTrp: 0.235 ± 0.143
0.47CysTyr: 0.47 ± 0.244
0.0CysXaa: 0.0 ± 0.0
Asp
5.722AspAla: 5.722 ± 0.522
0.314AspCys: 0.314 ± 0.196
3.998AspAsp: 3.998 ± 0.559
3.527AspGlu: 3.527 ± 0.488
2.9AspPhe: 2.9 ± 0.441
6.271AspGly: 6.271 ± 0.475
0.941AspHis: 0.941 ± 0.283
3.057AspIle: 3.057 ± 0.469
4.155AspLys: 4.155 ± 0.715
3.841AspLeu: 3.841 ± 0.629
1.881AspMet: 1.881 ± 0.353
2.43AspAsn: 2.43 ± 0.428
2.9AspPro: 2.9 ± 0.476
2.195AspGln: 2.195 ± 0.406
2.979AspArg: 2.979 ± 0.446
3.606AspSer: 3.606 ± 0.459
3.919AspThr: 3.919 ± 0.484
4.155AspVal: 4.155 ± 0.616
1.019AspTrp: 1.019 ± 0.303
2.273AspTyr: 2.273 ± 0.39
0.0AspXaa: 0.0 ± 0.0
Glu
7.055GluAla: 7.055 ± 0.732
0.705GluCys: 0.705 ± 0.294
4.468GluAsp: 4.468 ± 0.573
4.86GluGlu: 4.86 ± 0.796
2.9GluPhe: 2.9 ± 0.474
5.33GluGly: 5.33 ± 0.649
1.254GluHis: 1.254 ± 0.411
2.352GluIle: 2.352 ± 0.324
3.214GluLys: 3.214 ± 0.514
5.722GluLeu: 5.722 ± 0.863
1.568GluMet: 1.568 ± 0.483
2.038GluAsn: 2.038 ± 0.336
2.038GluPro: 2.038 ± 0.499
3.292GluGln: 3.292 ± 0.721
3.841GluArg: 3.841 ± 0.656
4.233GluSer: 4.233 ± 0.537
2.822GluThr: 2.822 ± 0.47
4.547GluVal: 4.547 ± 0.555
0.549GluTrp: 0.549 ± 0.201
2.665GluTyr: 2.665 ± 0.415
0.0GluXaa: 0.0 ± 0.0
Phe
2.665PheAla: 2.665 ± 0.509
0.392PheCys: 0.392 ± 0.211
2.9PheAsp: 2.9 ± 0.437
1.646PheGlu: 1.646 ± 0.312
0.941PhePhe: 0.941 ± 0.288
3.136PheGly: 3.136 ± 0.552
1.019PheHis: 1.019 ± 0.289
1.803PheIle: 1.803 ± 0.487
2.508PheLys: 2.508 ± 0.393
2.665PheLeu: 2.665 ± 0.512
0.941PheMet: 0.941 ± 0.222
2.273PheAsn: 2.273 ± 0.391
1.411PhePro: 1.411 ± 0.346
1.411PheGln: 1.411 ± 0.292
1.881PheArg: 1.881 ± 0.451
2.665PheSer: 2.665 ± 0.411
3.057PheThr: 3.057 ± 0.446
2.508PheVal: 2.508 ± 0.46
0.392PheTrp: 0.392 ± 0.159
0.941PheTyr: 0.941 ± 0.233
0.0PheXaa: 0.0 ± 0.0
Gly
7.369GlyAla: 7.369 ± 1.027
0.705GlyCys: 0.705 ± 0.216
5.566GlyAsp: 5.566 ± 0.574
5.409GlyGlu: 5.409 ± 0.627
2.508GlyPhe: 2.508 ± 0.386
6.193GlyGly: 6.193 ± 0.872
1.097GlyHis: 1.097 ± 0.336
5.017GlyIle: 5.017 ± 0.697
5.644GlyLys: 5.644 ± 0.717
6.349GlyLeu: 6.349 ± 0.657
1.725GlyMet: 1.725 ± 0.357
3.606GlyAsn: 3.606 ± 0.57
1.646GlyPro: 1.646 ± 0.431
2.508GlyGln: 2.508 ± 0.453
4.311GlyArg: 4.311 ± 0.37
5.958GlySer: 5.958 ± 0.815
5.017GlyThr: 5.017 ± 0.68
4.782GlyVal: 4.782 ± 0.736
1.646GlyTrp: 1.646 ± 0.37
3.057GlyTyr: 3.057 ± 0.48
0.0GlyXaa: 0.0 ± 0.0
His
0.941HisAla: 0.941 ± 0.286
0.47HisCys: 0.47 ± 0.201
1.019HisAsp: 1.019 ± 0.272
1.333HisGlu: 1.333 ± 0.349
0.784HisPhe: 0.784 ± 0.228
1.489HisGly: 1.489 ± 0.383
0.392HisHis: 0.392 ± 0.18
1.019HisIle: 1.019 ± 0.277
1.176HisLys: 1.176 ± 0.294
1.254HisLeu: 1.254 ± 0.394
0.627HisMet: 0.627 ± 0.208
0.314HisAsn: 0.314 ± 0.153
0.705HisPro: 0.705 ± 0.188
0.47HisGln: 0.47 ± 0.181
0.705HisArg: 0.705 ± 0.177
0.862HisSer: 0.862 ± 0.237
0.705HisThr: 0.705 ± 0.194
1.881HisVal: 1.881 ± 0.358
0.157HisTrp: 0.157 ± 0.103
0.941HisTyr: 0.941 ± 0.192
0.0HisXaa: 0.0 ± 0.0
Ile
4.39IleAla: 4.39 ± 0.503
0.784IleCys: 0.784 ± 0.212
3.214IleAsp: 3.214 ± 0.471
3.214IleGlu: 3.214 ± 0.53
1.097IlePhe: 1.097 ± 0.303
4.155IleGly: 4.155 ± 0.64
0.705IleHis: 0.705 ± 0.278
2.352IleIle: 2.352 ± 0.492
3.527IleLys: 3.527 ± 0.495
4.155IleLeu: 4.155 ± 0.493
1.097IleMet: 1.097 ± 0.333
2.43IleAsn: 2.43 ± 0.607
2.665IlePro: 2.665 ± 0.378
1.881IleGln: 1.881 ± 0.419
4.076IleArg: 4.076 ± 0.57
2.665IleSer: 2.665 ± 0.453
2.195IleThr: 2.195 ± 0.362
2.979IleVal: 2.979 ± 0.466
0.627IleTrp: 0.627 ± 0.217
1.568IleTyr: 1.568 ± 0.374
0.0IleXaa: 0.0 ± 0.0
Lys
7.525LysAla: 7.525 ± 1.02
0.314LysCys: 0.314 ± 0.135
3.841LysAsp: 3.841 ± 0.589
4.86LysGlu: 4.86 ± 0.714
2.116LysPhe: 2.116 ± 0.396
6.428LysGly: 6.428 ± 1.001
1.646LysHis: 1.646 ± 0.383
2.508LysIle: 2.508 ± 0.425
3.527LysLys: 3.527 ± 0.822
5.879LysLeu: 5.879 ± 0.592
1.568LysMet: 1.568 ± 0.366
2.665LysAsn: 2.665 ± 0.32
2.43LysPro: 2.43 ± 0.52
2.352LysGln: 2.352 ± 0.401
3.527LysArg: 3.527 ± 0.645
3.292LysSer: 3.292 ± 0.545
2.744LysThr: 2.744 ± 0.395
5.722LysVal: 5.722 ± 0.567
0.705LysTrp: 0.705 ± 0.229
1.725LysTyr: 1.725 ± 0.381
0.0LysXaa: 0.0 ± 0.0
Leu
7.76LeuAla: 7.76 ± 1.127
0.705LeuCys: 0.705 ± 0.263
4.468LeuAsp: 4.468 ± 0.535
6.349LeuGlu: 6.349 ± 0.924
2.665LeuPhe: 2.665 ± 0.417
5.174LeuGly: 5.174 ± 0.56
1.176LeuHis: 1.176 ± 0.299
4.155LeuIle: 4.155 ± 0.457
5.958LeuLys: 5.958 ± 0.626
6.114LeuLeu: 6.114 ± 0.827
2.038LeuMet: 2.038 ± 0.316
3.998LeuAsn: 3.998 ± 0.497
3.684LeuPro: 3.684 ± 0.446
3.841LeuGln: 3.841 ± 0.567
5.33LeuArg: 5.33 ± 0.595
4.703LeuSer: 4.703 ± 0.521
4.625LeuThr: 4.625 ± 0.527
4.782LeuVal: 4.782 ± 0.508
1.333LeuTrp: 1.333 ± 0.442
2.43LeuTyr: 2.43 ± 0.502
0.0LeuXaa: 0.0 ± 0.0
Met
2.979MetAla: 2.979 ± 0.38
0.235MetCys: 0.235 ± 0.134
2.038MetAsp: 2.038 ± 0.421
1.176MetGlu: 1.176 ± 0.25
0.862MetPhe: 0.862 ± 0.242
1.803MetGly: 1.803 ± 0.339
0.392MetHis: 0.392 ± 0.208
0.862MetIle: 0.862 ± 0.257
1.333MetLys: 1.333 ± 0.26
2.979MetLeu: 2.979 ± 0.408
0.705MetMet: 0.705 ± 0.22
1.019MetAsn: 1.019 ± 0.25
0.705MetPro: 0.705 ± 0.205
1.881MetGln: 1.881 ± 0.429
1.176MetArg: 1.176 ± 0.337
1.333MetSer: 1.333 ± 0.318
2.116MetThr: 2.116 ± 0.449
1.803MetVal: 1.803 ± 0.377
0.0MetTrp: 0.0 ± 0.0
0.549MetTyr: 0.549 ± 0.166
0.0MetXaa: 0.0 ± 0.0
Asn
3.292AsnAla: 3.292 ± 0.591
0.549AsnCys: 0.549 ± 0.243
1.803AsnAsp: 1.803 ± 0.299
2.587AsnGlu: 2.587 ± 0.509
1.489AsnPhe: 1.489 ± 0.343
4.703AsnGly: 4.703 ± 0.72
0.157AsnHis: 0.157 ± 0.1
3.214AsnIle: 3.214 ± 0.621
2.116AsnLys: 2.116 ± 0.365
3.057AsnLeu: 3.057 ± 0.467
1.176AsnMet: 1.176 ± 0.299
1.646AsnAsn: 1.646 ± 0.286
2.273AsnPro: 2.273 ± 0.382
1.725AsnGln: 1.725 ± 0.341
2.195AsnArg: 2.195 ± 0.592
3.214AsnSer: 3.214 ± 0.518
2.744AsnThr: 2.744 ± 0.514
3.057AsnVal: 3.057 ± 0.468
0.549AsnTrp: 0.549 ± 0.268
2.038AsnTyr: 2.038 ± 0.413
0.0AsnXaa: 0.0 ± 0.0
Pro
3.527ProAla: 3.527 ± 0.498
0.705ProCys: 0.705 ± 0.254
2.195ProAsp: 2.195 ± 0.307
3.841ProGlu: 3.841 ± 0.643
1.333ProPhe: 1.333 ± 0.285
2.587ProGly: 2.587 ± 0.398
0.549ProHis: 0.549 ± 0.195
1.097ProIle: 1.097 ± 0.272
2.43ProLys: 2.43 ± 0.381
2.744ProLeu: 2.744 ± 0.392
0.941ProMet: 0.941 ± 0.304
2.508ProAsn: 2.508 ± 0.401
0.784ProPro: 0.784 ± 0.313
1.333ProGln: 1.333 ± 0.263
2.195ProArg: 2.195 ± 0.37
2.273ProSer: 2.273 ± 0.408
1.881ProThr: 1.881 ± 0.373
2.43ProVal: 2.43 ± 0.299
0.705ProTrp: 0.705 ± 0.198
1.568ProTyr: 1.568 ± 0.338
0.0ProXaa: 0.0 ± 0.0
Gln
3.763GlnAla: 3.763 ± 0.575
0.157GlnCys: 0.157 ± 0.144
2.508GlnAsp: 2.508 ± 0.241
3.292GlnGlu: 3.292 ± 0.418
1.881GlnPhe: 1.881 ± 0.32
2.979GlnGly: 2.979 ± 0.475
0.549GlnHis: 0.549 ± 0.225
1.568GlnIle: 1.568 ± 0.386
2.9GlnLys: 2.9 ± 0.528
3.919GlnLeu: 3.919 ± 0.47
1.254GlnMet: 1.254 ± 0.409
1.489GlnAsn: 1.489 ± 0.201
1.489GlnPro: 1.489 ± 0.219
3.136GlnGln: 3.136 ± 0.603
2.352GlnArg: 2.352 ± 0.601
2.352GlnSer: 2.352 ± 0.412
1.568GlnThr: 1.568 ± 0.364
2.9GlnVal: 2.9 ± 0.441
1.097GlnTrp: 1.097 ± 0.273
1.489GlnTyr: 1.489 ± 0.464
0.0GlnXaa: 0.0 ± 0.0
Arg
5.252ArgAla: 5.252 ± 0.703
0.705ArgCys: 0.705 ± 0.264
3.449ArgAsp: 3.449 ± 0.471
3.684ArgGlu: 3.684 ± 0.479
2.195ArgPhe: 2.195 ± 0.528
3.606ArgGly: 3.606 ± 0.54
0.862ArgHis: 0.862 ± 0.292
3.136ArgIle: 3.136 ± 0.461
4.155ArgLys: 4.155 ± 0.653
4.938ArgLeu: 4.938 ± 0.656
1.254ArgMet: 1.254 ± 0.225
2.744ArgAsn: 2.744 ± 0.375
2.038ArgPro: 2.038 ± 0.339
2.587ArgGln: 2.587 ± 0.4
3.214ArgArg: 3.214 ± 0.43
3.998ArgSer: 3.998 ± 0.594
3.371ArgThr: 3.371 ± 0.712
3.057ArgVal: 3.057 ± 0.518
1.019ArgTrp: 1.019 ± 0.299
1.097ArgTyr: 1.097 ± 0.202
0.0ArgXaa: 0.0 ± 0.0
Ser
4.39SerAla: 4.39 ± 0.556
0.549SerCys: 0.549 ± 0.286
4.39SerAsp: 4.39 ± 0.603
3.684SerGlu: 3.684 ± 0.421
3.371SerPhe: 3.371 ± 0.462
4.625SerGly: 4.625 ± 0.533
1.803SerHis: 1.803 ± 0.353
3.057SerIle: 3.057 ± 0.473
4.39SerLys: 4.39 ± 0.519
4.468SerLeu: 4.468 ± 0.638
1.411SerMet: 1.411 ± 0.319
1.881SerAsn: 1.881 ± 0.453
1.881SerPro: 1.881 ± 0.314
2.822SerGln: 2.822 ± 0.448
3.136SerArg: 3.136 ± 0.467
3.292SerSer: 3.292 ± 0.562
4.468SerThr: 4.468 ± 0.783
4.233SerVal: 4.233 ± 0.571
0.862SerTrp: 0.862 ± 0.21
2.822SerTyr: 2.822 ± 0.519
0.0SerXaa: 0.0 ± 0.0
Thr
5.33ThrAla: 5.33 ± 0.765
0.862ThrCys: 0.862 ± 0.261
3.136ThrAsp: 3.136 ± 0.467
3.371ThrGlu: 3.371 ± 0.495
2.195ThrPhe: 2.195 ± 0.398
5.252ThrGly: 5.252 ± 0.536
1.176ThrHis: 1.176 ± 0.224
3.841ThrIle: 3.841 ± 0.519
4.547ThrLys: 4.547 ± 0.589
5.017ThrLeu: 5.017 ± 0.568
1.489ThrMet: 1.489 ± 0.311
1.803ThrAsn: 1.803 ± 0.463
2.665ThrPro: 2.665 ± 0.338
2.352ThrGln: 2.352 ± 0.481
2.195ThrArg: 2.195 ± 0.394
3.763ThrSer: 3.763 ± 0.601
3.606ThrThr: 3.606 ± 0.841
3.606ThrVal: 3.606 ± 0.683
0.941ThrTrp: 0.941 ± 0.271
1.568ThrTyr: 1.568 ± 0.401
0.0ThrXaa: 0.0 ± 0.0
Val
4.938ValAla: 4.938 ± 0.634
0.392ValCys: 0.392 ± 0.141
3.057ValAsp: 3.057 ± 0.322
4.311ValGlu: 4.311 ± 0.517
2.352ValPhe: 2.352 ± 0.517
4.86ValGly: 4.86 ± 0.558
1.097ValHis: 1.097 ± 0.322
3.684ValIle: 3.684 ± 0.543
4.155ValLys: 4.155 ± 0.572
6.036ValLeu: 6.036 ± 0.756
1.646ValMet: 1.646 ± 0.322
3.371ValAsn: 3.371 ± 0.591
2.587ValPro: 2.587 ± 0.417
2.665ValGln: 2.665 ± 0.33
4.311ValArg: 4.311 ± 0.579
4.86ValSer: 4.86 ± 0.767
5.801ValThr: 5.801 ± 0.721
4.86ValVal: 4.86 ± 0.672
0.862ValTrp: 0.862 ± 0.318
2.273ValTyr: 2.273 ± 0.446
0.0ValXaa: 0.0 ± 0.0
Trp
0.627TrpAla: 0.627 ± 0.218
0.47TrpCys: 0.47 ± 0.203
0.784TrpAsp: 0.784 ± 0.265
1.097TrpGlu: 1.097 ± 0.25
0.627TrpPhe: 0.627 ± 0.194
0.627TrpGly: 0.627 ± 0.195
0.47TrpHis: 0.47 ± 0.212
0.784TrpIle: 0.784 ± 0.292
1.254TrpLys: 1.254 ± 0.348
1.333TrpLeu: 1.333 ± 0.362
0.314TrpMet: 0.314 ± 0.165
0.784TrpAsn: 0.784 ± 0.225
0.549TrpPro: 0.549 ± 0.285
0.47TrpGln: 0.47 ± 0.167
0.784TrpArg: 0.784 ± 0.249
1.411TrpSer: 1.411 ± 0.357
0.705TrpThr: 0.705 ± 0.23
1.254TrpVal: 1.254 ± 0.316
0.157TrpTrp: 0.157 ± 0.11
0.078TrpTyr: 0.078 ± 0.066
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.273TyrAla: 2.273 ± 0.427
0.235TyrCys: 0.235 ± 0.126
2.587TyrAsp: 2.587 ± 0.533
1.803TyrGlu: 1.803 ± 0.326
1.176TyrPhe: 1.176 ± 0.287
2.9TyrGly: 2.9 ± 0.431
0.549TyrHis: 0.549 ± 0.255
1.411TyrIle: 1.411 ± 0.357
2.038TyrLys: 2.038 ± 0.325
2.273TyrLeu: 2.273 ± 0.406
1.333TyrMet: 1.333 ± 0.319
1.646TyrAsn: 1.646 ± 0.393
1.333TyrPro: 1.333 ± 0.348
1.725TyrGln: 1.725 ± 0.479
2.273TyrArg: 2.273 ± 0.427
1.411TyrSer: 1.411 ± 0.379
2.195TyrThr: 2.195 ± 0.444
2.822TyrVal: 2.822 ± 0.509
0.627TyrTrp: 0.627 ± 0.251
0.862TyrTyr: 0.862 ± 0.33
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 44 proteins (12758 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski