Pseudoxanthomonas suwonensis (strain 11-1)
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3068 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E6WVA4|E6WVA4_PSEUU Sulfate ABC transporter periplasmic sulfate-binding protein OS=Pseudoxanthomonas suwonensis (strain 11-1) OX=743721 GN=Psesu_2269 PE=4 SV=1
MM1 pKa = 7.76 KK2 pKa = 10.23 SFKK5 pKa = 10.38 LAASLAVLLSAAPFAAFAQEE25 pKa = 4.2 EE26 pKa = 4.36 EE27 pKa = 5.27 SPFSWEE33 pKa = 3.6 VTAVSDD39 pKa = 4.09 YY40 pKa = 9.8 VWRR43 pKa = 11.84 GVSQSDD49 pKa = 4.29 EE50 pKa = 4.43 EE51 pKa = 4.43 PTLQAGFTYY60 pKa = 9.83 TSPSGFYY67 pKa = 10.41 AGVWGSGVDD76 pKa = 4.54 FGPGDD81 pKa = 3.73 PNAEE85 pKa = 3.54 VDD87 pKa = 4.39 GFIGYY92 pKa = 7.65 NTDD95 pKa = 4.1 FSDD98 pKa = 3.87 SVNFDD103 pKa = 3.27 VMINRR108 pKa = 11.84 YY109 pKa = 6.56 TYY111 pKa = 10.49 PGASEE116 pKa = 5.27 LNFNEE121 pKa = 5.61 LITTTTFAEE130 pKa = 4.5 NYY132 pKa = 8.78 SLTVAYY138 pKa = 9.65 TNDD141 pKa = 3.38 FGGADD146 pKa = 3.19 AKK148 pKa = 10.73 AWYY151 pKa = 9.11 VAGGASFGLPNEE163 pKa = 4.29 FSLDD167 pKa = 3.65 LGVGRR172 pKa = 11.84 SLFDD176 pKa = 4.24 DD177 pKa = 5.53 DD178 pKa = 5.17 YY179 pKa = 12.02 SEE181 pKa = 6.46 DD182 pKa = 3.68 YY183 pKa = 10.65 TDD185 pKa = 3.12 WSVGLSRR192 pKa = 11.84 SWGLFSASLAYY203 pKa = 10.56 VGTDD207 pKa = 3.05 GNGRR211 pKa = 11.84 DD212 pKa = 4.07 LFGDD216 pKa = 3.87 LADD219 pKa = 4.38 DD220 pKa = 3.75 RR221 pKa = 11.84 VVLSLSIGQQ230 pKa = 3.56
Molecular weight: 24.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.605
IPC_protein 3.617
Toseland 3.389
ProMoST 3.795
Dawson 3.617
Bjellqvist 3.77
Wikipedia 3.567
Rodwell 3.439
Grimsley 3.3
Solomon 3.605
Lehninger 3.567
Nozaki 3.732
DTASelect 3.986
Thurlkill 3.452
EMBOSS 3.579
Sillero 3.745
Patrickios 0.693
IPC_peptide 3.592
IPC2_peptide 3.706
IPC2.peptide.svr19 3.675
Protein with the highest isoelectric point:
>tr|E6WPG4|E6WPG4_PSEUU Activator of Hsp90 ATPase 1 family protein OS=Pseudoxanthomonas suwonensis (strain 11-1) OX=743721 GN=Psesu_0347 PE=3 SV=1
MM1 pKa = 7.11 QAQSGNPRR9 pKa = 11.84 RR10 pKa = 11.84 SGPWASAVALLGGLAGLVFGRR31 pKa = 11.84 LVGSWLPEE39 pKa = 3.82 SPPWVGPGIVVGSLALLLVGTFLLPLFRR67 pKa = 11.84 RR68 pKa = 11.84 HH69 pKa = 5.62 RR70 pKa = 11.84 APRR73 pKa = 11.84 GRR75 pKa = 11.84 ARR77 pKa = 3.67
Molecular weight: 8.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.357
IPC2_protein 10.774
IPC_protein 12.384
Toseland 12.544
ProMoST 13.056
Dawson 12.544
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.047
Grimsley 12.588
Solomon 13.042
Lehninger 12.939
Nozaki 12.544
DTASelect 12.544
Thurlkill 12.544
EMBOSS 13.042
Sillero 12.544
Patrickios 11.798
IPC_peptide 13.042
IPC2_peptide 12.032
IPC2.peptide.svr19 9.193
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3068
0
3068
1012518
31
2310
330.0
35.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.395 ± 0.072
0.795 ± 0.015
5.682 ± 0.037
5.804 ± 0.046
3.231 ± 0.029
8.971 ± 0.035
2.185 ± 0.022
3.709 ± 0.036
2.355 ± 0.039
11.022 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.143 ± 0.021
2.244 ± 0.024
5.743 ± 0.038
3.707 ± 0.027
8.155 ± 0.045
4.836 ± 0.029
4.494 ± 0.028
7.679 ± 0.037
1.6 ± 0.023
2.248 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here