Hubei polero-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEP7|A0A1L3KEP7_9VIRU RNA-directed RNA polymerase OS=Hubei polero-like virus 2 OX=1923170 PE=4 SV=1
MM1 pKa = 7.36YY2 pKa = 9.26STVQTSSPDD11 pKa = 3.28LVGYY15 pKa = 8.63TEE17 pKa = 4.46TGHH20 pKa = 7.19LTLSWEE26 pKa = 4.21KK27 pKa = 10.12FQEE30 pKa = 4.09HH31 pKa = 6.18FKK33 pKa = 11.05VAVHH37 pKa = 6.0SLEE40 pKa = 4.04LDD42 pKa = 3.24AGIGIPYY49 pKa = 9.75IKK51 pKa = 10.06YY52 pKa = 9.79RR53 pKa = 11.84RR54 pKa = 11.84PTHH57 pKa = 6.25RR58 pKa = 11.84GWVEE62 pKa = 3.94DD63 pKa = 4.19PEE65 pKa = 4.58LLPVLAQMTFDD76 pKa = 4.44RR77 pKa = 11.84LQKK80 pKa = 9.49MSKK83 pKa = 9.91VFNNNLTPEE92 pKa = 4.12EE93 pKa = 4.24CVQLGLCDD101 pKa = 5.02PIRR104 pKa = 11.84VFIKK108 pKa = 10.73NEE110 pKa = 3.46PHH112 pKa = 6.19KK113 pKa = 10.44QSKK116 pKa = 10.16LDD118 pKa = 3.38EE119 pKa = 4.27GRR121 pKa = 11.84YY122 pKa = 8.96RR123 pKa = 11.84LIMSVSLVDD132 pKa = 3.45QLVARR137 pKa = 11.84ILFQRR142 pKa = 11.84QNKK145 pKa = 8.24MEE147 pKa = 4.34IEE149 pKa = 4.52LWRR152 pKa = 11.84SIPSKK157 pKa = 10.52PGFGLSTDD165 pKa = 3.45EE166 pKa = 4.08QTLEE170 pKa = 4.39FIKK173 pKa = 10.88LLSKK177 pKa = 10.19QANIEE182 pKa = 3.94AAEE185 pKa = 4.45LLTNWADD192 pKa = 3.58YY193 pKa = 10.31VVPTDD198 pKa = 3.9CSGFDD203 pKa = 3.27WSVSQWMLEE212 pKa = 3.9DD213 pKa = 3.35EE214 pKa = 4.43MEE216 pKa = 4.14VRR218 pKa = 11.84NRR220 pKa = 11.84LQSHH224 pKa = 5.95YY225 pKa = 10.62SGHH228 pKa = 5.29TEE230 pKa = 3.63KK231 pKa = 11.02QNFNLQQVWLHH242 pKa = 5.88CLGNSVLALSDD253 pKa = 3.4GRR255 pKa = 11.84LLAQEE260 pKa = 3.94VSGVQKK266 pKa = 10.55SGSYY270 pKa = 8.27NTSSTNSRR278 pKa = 11.84IRR280 pKa = 11.84VMAALHH286 pKa = 6.54CGASWAIAMGDD297 pKa = 3.76DD298 pKa = 4.56AIEE301 pKa = 4.67SNDD304 pKa = 3.71SNLEE308 pKa = 3.88LYY310 pKa = 10.02AQLGFKK316 pKa = 10.75VEE318 pKa = 4.12VSTKK322 pKa = 10.89LEE324 pKa = 4.12FCSHH328 pKa = 6.21IFEE331 pKa = 4.94KK332 pKa = 11.08LNLAIPINKK341 pKa = 9.56GKK343 pKa = 8.85MLYY346 pKa = 10.43KK347 pKa = 10.66LIFGYY352 pKa = 10.7DD353 pKa = 3.51PGCGNVEE360 pKa = 4.12VVTNYY365 pKa = 10.64LNALWSVLHH374 pKa = 6.06EE375 pKa = 4.37LRR377 pKa = 11.84YY378 pKa = 10.78DD379 pKa = 3.75PDD381 pKa = 3.58LVSRR385 pKa = 11.84LHH387 pKa = 6.07KK388 pKa = 10.26WLVPVV393 pKa = 4.26

Molecular weight: 44.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEI1|A0A1L3KEI1_9VIRU Uncharacterized protein OS=Hubei polero-like virus 2 OX=1923170 PE=4 SV=1
MM1 pKa = 6.74NTGGARR7 pKa = 11.84RR8 pKa = 11.84KK9 pKa = 9.61GRR11 pKa = 11.84NGKK14 pKa = 7.81RR15 pKa = 11.84TRR17 pKa = 11.84TGRR20 pKa = 11.84ARR22 pKa = 11.84RR23 pKa = 11.84NPVRR27 pKa = 11.84PIVMVAPTRR36 pKa = 11.84PTWRR40 pKa = 11.84NRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84PTRR47 pKa = 11.84SNRR50 pKa = 11.84GRR52 pKa = 11.84GQLRR56 pKa = 11.84NAGGTSNSEE65 pKa = 3.71TFVFNKK71 pKa = 10.35DD72 pKa = 3.42SLKK75 pKa = 10.93GNSKK79 pKa = 8.85GTITFGPSLSEE90 pKa = 4.12SVALSGGVLKK100 pKa = 10.54AYY102 pKa = 10.29HH103 pKa = 6.77EE104 pKa = 4.24YY105 pKa = 10.75KK106 pKa = 8.49ITKK109 pKa = 8.89VNVRR113 pKa = 11.84FFSEE117 pKa = 4.29SASTAEE123 pKa = 3.84GSIAYY128 pKa = 9.14EE129 pKa = 3.89VDD131 pKa = 3.31PHH133 pKa = 7.13CKK135 pKa = 9.06LTEE138 pKa = 4.42LGSTLRR144 pKa = 11.84KK145 pKa = 8.51FTITKK150 pKa = 10.07AGQATFGASKK160 pKa = 10.57INGLEE165 pKa = 3.83WHH167 pKa = 7.31DD168 pKa = 4.0SSEE171 pKa = 4.33DD172 pKa = 3.39QFKK175 pKa = 10.28IHH177 pKa = 6.23YY178 pKa = 8.62KK179 pKa = 10.77GNGSTTTAGYY189 pKa = 8.81FQIRR193 pKa = 11.84YY194 pKa = 7.38WVSLHH199 pKa = 6.02NPKK202 pKa = 10.6

Molecular weight: 22.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1847

202

726

369.4

41.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.793 ± 0.414

1.678 ± 0.454

4.602 ± 0.796

6.172 ± 0.383

3.844 ± 0.343

6.497 ± 0.633

2.382 ± 0.154

6.064 ± 0.821

5.793 ± 0.662

9.258 ± 0.974

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.949 ± 0.23

5.143 ± 0.235

5.468 ± 0.592

3.952 ± 0.471

5.522 ± 1.126

8.988 ± 0.427

6.605 ± 1.318

5.468 ± 0.592

1.462 ± 0.232

3.357 ± 0.458
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski