Amino acid dipepetide frequency for Mycobacterium phage Niza

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.184AlaAla: 12.184 ± 1.306
0.746AlaCys: 0.746 ± 0.195
6.216AlaAsp: 6.216 ± 0.687
6.527AlaGlu: 6.527 ± 0.775
3.232AlaPhe: 3.232 ± 0.471
7.149AlaGly: 7.149 ± 0.581
1.741AlaHis: 1.741 ± 0.329
3.916AlaIle: 3.916 ± 0.543
4.289AlaLys: 4.289 ± 0.499
8.765AlaLeu: 8.765 ± 0.82
2.176AlaMet: 2.176 ± 0.37
2.549AlaAsn: 2.549 ± 0.334
5.532AlaPro: 5.532 ± 0.668
2.922AlaGln: 2.922 ± 0.521
6.092AlaArg: 6.092 ± 0.538
4.662AlaSer: 4.662 ± 0.547
5.905AlaThr: 5.905 ± 0.619
9.013AlaVal: 9.013 ± 0.839
1.678AlaTrp: 1.678 ± 0.318
2.797AlaTyr: 2.797 ± 0.453
0.0AlaXaa: 0.0 ± 0.0
Cys
0.746CysAla: 0.746 ± 0.243
0.124CysCys: 0.124 ± 0.087
0.746CysAsp: 0.746 ± 0.213
1.057CysGlu: 1.057 ± 0.344
0.186CysPhe: 0.186 ± 0.106
0.808CysGly: 0.808 ± 0.286
0.124CysHis: 0.124 ± 0.077
0.373CysIle: 0.373 ± 0.166
0.311CysLys: 0.311 ± 0.145
0.373CysLeu: 0.373 ± 0.174
0.124CysMet: 0.124 ± 0.08
0.497CysAsn: 0.497 ± 0.156
0.373CysPro: 0.373 ± 0.161
0.311CysGln: 0.311 ± 0.143
0.559CysArg: 0.559 ± 0.205
0.311CysSer: 0.311 ± 0.147
0.249CysThr: 0.249 ± 0.126
0.249CysVal: 0.249 ± 0.118
0.186CysTrp: 0.186 ± 0.11
0.249CysTyr: 0.249 ± 0.178
0.0CysXaa: 0.0 ± 0.0
Asp
5.968AspAla: 5.968 ± 0.714
0.622AspCys: 0.622 ± 0.181
4.911AspAsp: 4.911 ± 0.546
3.543AspGlu: 3.543 ± 0.416
2.3AspPhe: 2.3 ± 0.305
6.03AspGly: 6.03 ± 0.685
1.057AspHis: 1.057 ± 0.246
2.673AspIle: 2.673 ± 0.453
2.549AspLys: 2.549 ± 0.418
6.838AspLeu: 6.838 ± 0.672
1.181AspMet: 1.181 ± 0.234
1.865AspAsn: 1.865 ± 0.316
4.786AspPro: 4.786 ± 0.456
1.554AspGln: 1.554 ± 0.349
4.165AspArg: 4.165 ± 0.542
2.859AspSer: 2.859 ± 0.452
3.978AspThr: 3.978 ± 0.425
4.165AspVal: 4.165 ± 0.489
1.741AspTrp: 1.741 ± 0.353
2.176AspTyr: 2.176 ± 0.325
0.0AspXaa: 0.0 ± 0.0
Glu
6.092GluAla: 6.092 ± 0.777
0.373GluCys: 0.373 ± 0.23
4.165GluAsp: 4.165 ± 0.48
4.786GluGlu: 4.786 ± 0.505
2.051GluPhe: 2.051 ± 0.351
4.414GluGly: 4.414 ± 0.457
1.305GluHis: 1.305 ± 0.273
3.605GluIle: 3.605 ± 0.488
2.984GluLys: 2.984 ± 0.387
6.838GluLeu: 6.838 ± 0.601
1.492GluMet: 1.492 ± 0.245
1.554GluAsn: 1.554 ± 0.378
2.238GluPro: 2.238 ± 0.409
2.549GluGln: 2.549 ± 0.382
3.854GluArg: 3.854 ± 0.555
3.357GluSer: 3.357 ± 0.394
3.916GluThr: 3.916 ± 0.531
5.781GluVal: 5.781 ± 0.622
1.492GluTrp: 1.492 ± 0.308
2.797GluTyr: 2.797 ± 0.513
0.0GluXaa: 0.0 ± 0.0
Phe
2.238PheAla: 2.238 ± 0.334
0.311PheCys: 0.311 ± 0.153
2.673PheAsp: 2.673 ± 0.317
1.865PheGlu: 1.865 ± 0.328
0.622PhePhe: 0.622 ± 0.177
3.481PheGly: 3.481 ± 0.474
0.497PheHis: 0.497 ± 0.232
1.368PheIle: 1.368 ± 0.294
1.243PheLys: 1.243 ± 0.25
2.238PheLeu: 2.238 ± 0.428
0.622PheMet: 0.622 ± 0.197
1.057PheAsn: 1.057 ± 0.266
1.43PhePro: 1.43 ± 0.292
0.87PheGln: 0.87 ± 0.214
2.051PheArg: 2.051 ± 0.359
2.114PheSer: 2.114 ± 0.414
1.927PheThr: 1.927 ± 0.373
2.3PheVal: 2.3 ± 0.349
0.622PheTrp: 0.622 ± 0.186
0.932PheTyr: 0.932 ± 0.25
0.0PheXaa: 0.0 ± 0.0
Gly
7.522GlyAla: 7.522 ± 0.967
0.622GlyCys: 0.622 ± 0.186
5.905GlyAsp: 5.905 ± 0.572
4.351GlyGlu: 4.351 ± 0.455
2.797GlyPhe: 2.797 ± 0.411
8.578GlyGly: 8.578 ± 1.572
1.865GlyHis: 1.865 ± 0.336
4.662GlyIle: 4.662 ± 0.598
3.605GlyLys: 3.605 ± 0.436
7.522GlyLeu: 7.522 ± 0.757
1.927GlyMet: 1.927 ± 0.307
3.232GlyAsn: 3.232 ± 0.41
4.041GlyPro: 4.041 ± 0.563
2.3GlyGln: 2.3 ± 0.341
5.408GlyArg: 5.408 ± 0.547
6.092GlySer: 6.092 ± 0.819
4.911GlyThr: 4.911 ± 0.494
5.408GlyVal: 5.408 ± 0.607
2.673GlyTrp: 2.673 ± 0.392
2.984GlyTyr: 2.984 ± 0.412
0.0GlyXaa: 0.0 ± 0.0
His
1.741HisAla: 1.741 ± 0.405
0.186HisCys: 0.186 ± 0.163
1.368HisAsp: 1.368 ± 0.274
1.554HisGlu: 1.554 ± 0.324
0.559HisPhe: 0.559 ± 0.192
1.803HisGly: 1.803 ± 0.381
0.746HisHis: 0.746 ± 0.224
1.119HisIle: 1.119 ± 0.223
0.995HisLys: 0.995 ± 0.272
1.243HisLeu: 1.243 ± 0.294
0.124HisMet: 0.124 ± 0.083
0.249HisAsn: 0.249 ± 0.133
1.119HisPro: 1.119 ± 0.25
0.87HisGln: 0.87 ± 0.206
1.492HisArg: 1.492 ± 0.259
0.746HisSer: 0.746 ± 0.182
1.119HisThr: 1.119 ± 0.234
1.616HisVal: 1.616 ± 0.27
0.435HisTrp: 0.435 ± 0.129
0.684HisTyr: 0.684 ± 0.224
0.0HisXaa: 0.0 ± 0.0
Ile
6.465IleAla: 6.465 ± 0.756
0.373IleCys: 0.373 ± 0.153
3.17IleAsp: 3.17 ± 0.385
3.854IleGlu: 3.854 ± 0.463
0.932IlePhe: 0.932 ± 0.257
4.041IleGly: 4.041 ± 0.462
0.932IleHis: 0.932 ± 0.2
1.616IleIle: 1.616 ± 0.297
1.554IleLys: 1.554 ± 0.339
3.481IleLeu: 3.481 ± 0.454
0.808IleMet: 0.808 ± 0.203
1.927IleAsn: 1.927 ± 0.323
3.17IlePro: 3.17 ± 0.415
1.368IleGln: 1.368 ± 0.388
3.605IleArg: 3.605 ± 0.494
3.605IleSer: 3.605 ± 0.416
3.295IleThr: 3.295 ± 0.41
3.357IleVal: 3.357 ± 0.503
0.932IleTrp: 0.932 ± 0.201
1.616IleTyr: 1.616 ± 0.303
0.0IleXaa: 0.0 ± 0.0
Lys
3.916LysAla: 3.916 ± 0.513
0.435LysCys: 0.435 ± 0.207
2.673LysAsp: 2.673 ± 0.457
2.051LysGlu: 2.051 ± 0.41
1.43LysPhe: 1.43 ± 0.285
2.735LysGly: 2.735 ± 0.391
1.243LysHis: 1.243 ± 0.358
2.424LysIle: 2.424 ± 0.397
1.803LysLys: 1.803 ± 0.327
3.543LysLeu: 3.543 ± 0.445
0.932LysMet: 0.932 ± 0.25
1.678LysAsn: 1.678 ± 0.281
2.611LysPro: 2.611 ± 0.428
1.43LysGln: 1.43 ± 0.268
3.668LysArg: 3.668 ± 0.651
2.549LysSer: 2.549 ± 0.409
2.424LysThr: 2.424 ± 0.424
3.481LysVal: 3.481 ± 0.439
0.808LysTrp: 0.808 ± 0.215
1.181LysTyr: 1.181 ± 0.312
0.0LysXaa: 0.0 ± 0.0
Leu
8.454LeuAla: 8.454 ± 0.779
0.435LeuCys: 0.435 ± 0.204
5.595LeuAsp: 5.595 ± 0.508
5.408LeuGlu: 5.408 ± 0.567
2.176LeuPhe: 2.176 ± 0.401
7.397LeuGly: 7.397 ± 0.746
1.43LeuHis: 1.43 ± 0.293
4.786LeuIle: 4.786 ± 0.518
4.103LeuLys: 4.103 ± 0.585
5.284LeuLeu: 5.284 ± 0.534
1.741LeuMet: 1.741 ± 0.297
2.797LeuAsn: 2.797 ± 0.396
5.47LeuPro: 5.47 ± 0.626
2.549LeuGln: 2.549 ± 0.433
5.595LeuArg: 5.595 ± 0.521
5.905LeuSer: 5.905 ± 0.581
6.341LeuThr: 6.341 ± 0.493
4.6LeuVal: 4.6 ± 0.571
0.932LeuTrp: 0.932 ± 0.329
2.549LeuTyr: 2.549 ± 0.409
0.0LeuXaa: 0.0 ± 0.0
Met
2.549MetAla: 2.549 ± 0.365
0.0MetCys: 0.0 ± 0.0
1.119MetAsp: 1.119 ± 0.24
1.368MetGlu: 1.368 ± 0.299
0.622MetPhe: 0.622 ± 0.211
1.305MetGly: 1.305 ± 0.281
0.373MetHis: 0.373 ± 0.24
0.373MetIle: 0.373 ± 0.137
1.181MetLys: 1.181 ± 0.251
1.119MetLeu: 1.119 ± 0.283
0.124MetMet: 0.124 ± 0.084
1.119MetAsn: 1.119 ± 0.218
1.119MetPro: 1.119 ± 0.26
0.808MetGln: 0.808 ± 0.195
1.368MetArg: 1.368 ± 0.327
2.362MetSer: 2.362 ± 0.386
1.803MetThr: 1.803 ± 0.29
0.995MetVal: 0.995 ± 0.253
0.249MetTrp: 0.249 ± 0.108
0.373MetTyr: 0.373 ± 0.155
0.0MetXaa: 0.0 ± 0.0
Asn
3.357AsnAla: 3.357 ± 0.445
0.0AsnCys: 0.0 ± 0.0
1.989AsnAsp: 1.989 ± 0.36
1.989AsnGlu: 1.989 ± 0.398
0.87AsnPhe: 0.87 ± 0.238
3.295AsnGly: 3.295 ± 0.486
0.746AsnHis: 0.746 ± 0.211
1.616AsnIle: 1.616 ± 0.295
0.684AsnLys: 0.684 ± 0.211
2.486AsnLeu: 2.486 ± 0.352
0.559AsnMet: 0.559 ± 0.167
0.808AsnAsn: 0.808 ± 0.228
2.859AsnPro: 2.859 ± 0.348
1.181AsnGln: 1.181 ± 0.265
1.616AsnArg: 1.616 ± 0.345
1.554AsnSer: 1.554 ± 0.424
1.927AsnThr: 1.927 ± 0.325
2.3AsnVal: 2.3 ± 0.424
0.622AsnTrp: 0.622 ± 0.186
1.181AsnTyr: 1.181 ± 0.265
0.0AsnXaa: 0.0 ± 0.0
Pro
5.035ProAla: 5.035 ± 0.561
0.311ProCys: 0.311 ± 0.144
4.476ProAsp: 4.476 ± 0.491
4.289ProGlu: 4.289 ± 0.495
1.865ProPhe: 1.865 ± 0.358
5.657ProGly: 5.657 ± 0.557
0.808ProHis: 0.808 ± 0.218
2.362ProIle: 2.362 ± 0.382
2.3ProLys: 2.3 ± 0.35
4.351ProLeu: 4.351 ± 0.497
0.808ProMet: 0.808 ± 0.287
1.616ProAsn: 1.616 ± 0.308
2.922ProPro: 2.922 ± 0.425
1.492ProGln: 1.492 ± 0.347
2.797ProArg: 2.797 ± 0.479
3.73ProSer: 3.73 ± 0.489
4.414ProThr: 4.414 ± 0.547
3.73ProVal: 3.73 ± 0.444
0.808ProTrp: 0.808 ± 0.295
1.678ProTyr: 1.678 ± 0.381
0.0ProXaa: 0.0 ± 0.0
Gln
3.108GlnAla: 3.108 ± 0.435
0.062GlnCys: 0.062 ± 0.06
1.243GlnAsp: 1.243 ± 0.267
1.989GlnGlu: 1.989 ± 0.329
0.932GlnPhe: 0.932 ± 0.21
2.486GlnGly: 2.486 ± 0.376
0.622GlnHis: 0.622 ± 0.184
2.922GlnIle: 2.922 ± 0.524
1.181GlnLys: 1.181 ± 0.247
3.854GlnLeu: 3.854 ± 0.544
1.057GlnMet: 1.057 ± 0.245
0.435GlnAsn: 0.435 ± 0.155
2.176GlnPro: 2.176 ± 0.424
1.989GlnGln: 1.989 ± 0.433
1.678GlnArg: 1.678 ± 0.387
1.741GlnSer: 1.741 ± 0.276
1.616GlnThr: 1.616 ± 0.292
2.424GlnVal: 2.424 ± 0.309
0.559GlnTrp: 0.559 ± 0.173
0.497GlnTyr: 0.497 ± 0.162
0.0GlnXaa: 0.0 ± 0.0
Arg
5.346ArgAla: 5.346 ± 0.593
1.057ArgCys: 1.057 ± 0.298
2.984ArgAsp: 2.984 ± 0.482
5.035ArgGlu: 5.035 ± 0.667
1.803ArgPhe: 1.803 ± 0.393
5.284ArgGly: 5.284 ± 0.53
1.119ArgHis: 1.119 ± 0.267
3.17ArgIle: 3.17 ± 0.483
3.73ArgLys: 3.73 ± 0.51
5.843ArgLeu: 5.843 ± 0.66
1.989ArgMet: 1.989 ± 0.291
2.362ArgAsn: 2.362 ± 0.42
2.424ArgPro: 2.424 ± 0.372
1.927ArgGln: 1.927 ± 0.358
5.47ArgArg: 5.47 ± 0.788
3.481ArgSer: 3.481 ± 0.499
3.481ArgThr: 3.481 ± 0.494
5.284ArgVal: 5.284 ± 0.579
1.243ArgTrp: 1.243 ± 0.242
1.616ArgTyr: 1.616 ± 0.25
0.0ArgXaa: 0.0 ± 0.0
Ser
5.719SerAla: 5.719 ± 0.654
0.746SerCys: 0.746 ± 0.22
2.797SerAsp: 2.797 ± 0.39
4.227SerGlu: 4.227 ± 0.6
2.176SerPhe: 2.176 ± 0.431
6.403SerGly: 6.403 ± 0.703
1.43SerHis: 1.43 ± 0.292
2.797SerIle: 2.797 ± 0.375
2.424SerLys: 2.424 ± 0.383
4.973SerLeu: 4.973 ± 0.538
1.616SerMet: 1.616 ± 0.277
2.362SerAsn: 2.362 ± 0.346
3.108SerPro: 3.108 ± 0.471
1.989SerGln: 1.989 ± 0.321
2.611SerArg: 2.611 ± 0.329
2.984SerSer: 2.984 ± 0.589
2.922SerThr: 2.922 ± 0.445
3.916SerVal: 3.916 ± 0.43
1.368SerTrp: 1.368 ± 0.306
1.865SerTyr: 1.865 ± 0.356
0.0SerXaa: 0.0 ± 0.0
Thr
7.086ThrAla: 7.086 ± 0.882
0.311ThrCys: 0.311 ± 0.149
4.662ThrAsp: 4.662 ± 0.548
3.792ThrGlu: 3.792 ± 0.44
2.114ThrPhe: 2.114 ± 0.377
6.403ThrGly: 6.403 ± 0.596
1.119ThrHis: 1.119 ± 0.272
3.232ThrIle: 3.232 ± 0.622
2.797ThrLys: 2.797 ± 0.412
5.657ThrLeu: 5.657 ± 0.653
0.746ThrMet: 0.746 ± 0.188
1.554ThrAsn: 1.554 ± 0.285
3.543ThrPro: 3.543 ± 0.5
2.114ThrGln: 2.114 ± 0.394
3.481ThrArg: 3.481 ± 0.511
3.17ThrSer: 3.17 ± 0.53
4.103ThrThr: 4.103 ± 0.503
5.097ThrVal: 5.097 ± 0.585
1.305ThrTrp: 1.305 ± 0.276
1.803ThrTyr: 1.803 ± 0.355
0.0ThrXaa: 0.0 ± 0.0
Val
6.651ValAla: 6.651 ± 0.606
0.435ValCys: 0.435 ± 0.156
5.408ValAsp: 5.408 ± 0.459
4.786ValGlu: 4.786 ± 0.45
2.424ValPhe: 2.424 ± 0.424
4.414ValGly: 4.414 ± 0.749
1.616ValHis: 1.616 ± 0.27
3.854ValIle: 3.854 ± 0.444
3.295ValLys: 3.295 ± 0.495
5.035ValLeu: 5.035 ± 0.553
1.243ValMet: 1.243 ± 0.352
2.424ValAsn: 2.424 ± 0.361
4.289ValPro: 4.289 ± 0.54
2.424ValGln: 2.424 ± 0.401
5.035ValArg: 5.035 ± 0.622
4.724ValSer: 4.724 ± 0.508
5.843ValThr: 5.843 ± 0.652
5.222ValVal: 5.222 ± 0.588
1.305ValTrp: 1.305 ± 0.272
2.238ValTyr: 2.238 ± 0.411
0.0ValXaa: 0.0 ± 0.0
Trp
1.492TrpAla: 1.492 ± 0.325
0.249TrpCys: 0.249 ± 0.117
1.43TrpAsp: 1.43 ± 0.26
0.932TrpGlu: 0.932 ± 0.228
0.684TrpPhe: 0.684 ± 0.236
1.989TrpGly: 1.989 ± 0.347
0.311TrpHis: 0.311 ± 0.145
1.243TrpIle: 1.243 ± 0.21
0.435TrpLys: 0.435 ± 0.238
1.865TrpLeu: 1.865 ± 0.294
0.373TrpMet: 0.373 ± 0.169
0.373TrpAsn: 0.373 ± 0.142
0.684TrpPro: 0.684 ± 0.214
0.87TrpGln: 0.87 ± 0.212
1.368TrpArg: 1.368 ± 0.298
0.995TrpSer: 0.995 ± 0.269
1.803TrpThr: 1.803 ± 0.359
1.989TrpVal: 1.989 ± 0.301
0.497TrpTrp: 0.497 ± 0.195
0.435TrpTyr: 0.435 ± 0.178
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.424TyrAla: 2.424 ± 0.379
0.559TyrCys: 0.559 ± 0.207
1.43TyrAsp: 1.43 ± 0.3
2.238TyrGlu: 2.238 ± 0.338
0.684TyrPhe: 0.684 ± 0.22
2.735TyrGly: 2.735 ± 0.452
0.684TyrHis: 0.684 ± 0.193
1.989TyrIle: 1.989 ± 0.368
1.554TyrLys: 1.554 ± 0.333
2.3TyrLeu: 2.3 ± 0.36
0.622TyrMet: 0.622 ± 0.158
1.181TyrAsn: 1.181 ± 0.3
1.554TyrPro: 1.554 ± 0.278
1.057TyrGln: 1.057 ± 0.249
2.735TyrArg: 2.735 ± 0.42
1.492TyrSer: 1.492 ± 0.278
2.114TyrThr: 2.114 ± 0.383
1.741TyrVal: 1.741 ± 0.329
0.559TyrTrp: 0.559 ± 0.173
0.559TyrTyr: 0.559 ± 0.195
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 89 proteins (16088 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski