Streptococcus satellite phage Javan417
Average proteome isoelectric point is 7.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZNC1|A0A4D5ZNC1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan417 OX=2558692 GN=JavanS417_0004 PE=4 SV=1
MM1 pKa = 7.48 SIVSLRR7 pKa = 11.84 LNKK10 pKa = 10.21 NEE12 pKa = 3.77 EE13 pKa = 4.33 EE14 pKa = 4.2 LFKK17 pKa = 10.9 SYY19 pKa = 10.64 SIHH22 pKa = 5.72 TGKK25 pKa = 8.17 TLSEE29 pKa = 4.13 LFKK32 pKa = 10.45 TALAEE37 pKa = 4.15 QIEE40 pKa = 4.33 DD41 pKa = 3.44 QLDD44 pKa = 3.72 YY45 pKa = 10.39 EE46 pKa = 4.58 TGIKK50 pKa = 10.08 ALNEE54 pKa = 4.05 FKK56 pKa = 10.79 KK57 pKa = 10.9 NPVTHH62 pKa = 6.84 SIDD65 pKa = 4.45 DD66 pKa = 4.16 IIEE69 pKa = 4.08 EE70 pKa = 4.5 FEE72 pKa = 4.02 NDD74 pKa = 3.17 LL75 pKa = 4.57
Molecular weight: 8.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.676
IPC2_protein 4.622
IPC_protein 4.469
Toseland 4.342
ProMoST 4.571
Dawson 4.406
Bjellqvist 4.558
Wikipedia 4.266
Rodwell 4.317
Grimsley 4.253
Solomon 4.406
Lehninger 4.355
Nozaki 4.52
DTASelect 4.622
Thurlkill 4.342
EMBOSS 4.279
Sillero 4.584
Patrickios 4.05
IPC_peptide 4.418
IPC2_peptide 4.584
IPC2.peptide.svr19 4.509
Protein with the highest isoelectric point:
>tr|A0A4D5ZN19|A0A4D5ZN19_9VIRU RelB/StbD replicon stabilization protein OS=Streptococcus satellite phage Javan417 OX=2558692 GN=JavanS417_0015 PE=4 SV=1
MM1 pKa = 6.72 MTTFTKK7 pKa = 10.39 EE8 pKa = 3.75 FQQQLLEE15 pKa = 4.21 QINGVLQEE23 pKa = 4.03 RR24 pKa = 11.84 VQIEE28 pKa = 3.91 NEE30 pKa = 3.8 RR31 pKa = 11.84 QKK33 pKa = 11.17 RR34 pKa = 11.84 KK35 pKa = 7.82 TKK37 pKa = 10.75 ANRR40 pKa = 11.84 KK41 pKa = 7.82 YY42 pKa = 10.61 GKK44 pKa = 10.02 KK45 pKa = 10.07 RR46 pKa = 11.84 GG47 pKa = 3.61
Molecular weight: 5.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.199
IPC2_protein 9.633
IPC_protein 9.838
Toseland 10.789
ProMoST 10.277
Dawson 10.847
Bjellqvist 10.438
Wikipedia 10.965
Rodwell 11.359
Grimsley 10.862
Solomon 10.921
Lehninger 10.906
Nozaki 10.745
DTASelect 10.438
Thurlkill 10.745
EMBOSS 11.155
Sillero 10.76
Patrickios 11.14
IPC_peptide 10.921
IPC2_peptide 8.843
IPC2.peptide.svr19 8.745
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
16
0
16
2084
39
387
130.3
15.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.806 ± 0.742
0.528 ± 0.19
5.662 ± 0.654
8.493 ± 0.96
3.503 ± 0.544
4.463 ± 0.447
1.488 ± 0.355
6.574 ± 0.619
11.132 ± 0.461
9.933 ± 0.861
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.399 ± 0.323
6.286 ± 0.558
2.159 ± 0.213
4.415 ± 0.463
4.894 ± 0.507
4.463 ± 0.573
6.43 ± 0.739
5.134 ± 0.626
1.2 ± 0.337
5.038 ± 0.379
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here