Vibrio phage Va_178/90_p41
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 90 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A513SP79|A0A513SP79_9CAUD UPF0547 domain-containing protein OS=Vibrio phage Va_178/90_p41 OX=2565518 PE=4 SV=1
MM1 pKa = 7.81 VPIRR5 pKa = 11.84 DD6 pKa = 3.47 VLLTHH11 pKa = 6.69 EE12 pKa = 5.1 LSNGAKK18 pKa = 10.26 RR19 pKa = 11.84 NGDD22 pKa = 2.67 NKK24 pKa = 9.93 MFHH27 pKa = 6.57 TSPKK31 pKa = 9.68 AIEE34 pKa = 4.0 IGTINKK40 pKa = 9.85 YY41 pKa = 10.16 GVAGDD46 pKa = 4.4 CLFFSDD52 pKa = 5.83 DD53 pKa = 3.73 IYY55 pKa = 11.26 IMTASRR61 pKa = 11.84 TCYY64 pKa = 10.29 VYY66 pKa = 10.58 EE67 pKa = 5.15 ADD69 pKa = 4.1 FDD71 pKa = 4.47 CVRR74 pKa = 11.84 ASQLYY79 pKa = 9.96 DD80 pKa = 3.54 DD81 pKa = 5.78 EE82 pKa = 6.77 IIADD86 pKa = 3.57 IARR89 pKa = 11.84 YY90 pKa = 9.43 FDD92 pKa = 3.66 VDD94 pKa = 3.16 EE95 pKa = 4.6 STAEE99 pKa = 4.03 SLLDD103 pKa = 3.35 GTQNEE108 pKa = 4.29 WCLDD112 pKa = 3.67 DD113 pKa = 5.49 FDD115 pKa = 6.54 CEE117 pKa = 5.18 GEE119 pKa = 4.17 NSWWLQGKK127 pKa = 9.96 RR128 pKa = 11.84 GEE130 pKa = 4.64 CAVKK134 pKa = 9.78 MGYY137 pKa = 9.76 DD138 pKa = 3.25 GCEE141 pKa = 4.25 DD142 pKa = 3.79 EE143 pKa = 6.04 DD144 pKa = 4.1 EE145 pKa = 4.34 QGTVYY150 pKa = 10.25 IVPMAGRR157 pKa = 11.84 EE158 pKa = 4.22 SEE160 pKa = 4.29 LKK162 pKa = 10.7 LVEE165 pKa = 4.43 KK166 pKa = 10.58 KK167 pKa = 10.61
Molecular weight: 18.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.086
IPC2_protein 4.177
IPC_protein 4.139
Toseland 3.948
ProMoST 4.279
Dawson 4.113
Bjellqvist 4.266
Wikipedia 4.012
Rodwell 3.973
Grimsley 3.859
Solomon 4.113
Lehninger 4.062
Nozaki 4.228
DTASelect 4.431
Thurlkill 3.973
EMBOSS 4.024
Sillero 4.253
Patrickios 2.969
IPC_peptide 4.113
IPC2_peptide 4.24
IPC2.peptide.svr19 4.181
Protein with the highest isoelectric point:
>tr|A0A513SP45|A0A513SP45_9CAUD Uncharacterized protein OS=Vibrio phage Va_178/90_p41 OX=2565518 PE=4 SV=1
MM1 pKa = 7.32 SCAIKK6 pKa = 9.8 TKK8 pKa = 10.24 NDD10 pKa = 3.68 NIHH13 pKa = 5.93 TPRR16 pKa = 11.84 DD17 pKa = 3.38 FRR19 pKa = 11.84 GPRR22 pKa = 11.84 NVFVNGNKK30 pKa = 9.64 IKK32 pKa = 10.48 FVVYY36 pKa = 10.76 ADD38 pKa = 3.63 TKK40 pKa = 10.11 KK41 pKa = 11.1 GIVKK45 pKa = 7.46 YY46 pKa = 9.88 HH47 pKa = 5.54 PHH49 pKa = 5.85 PVRR52 pKa = 11.84 RR53 pKa = 11.84 KK54 pKa = 7.87 RR55 pKa = 11.84 GSNVVYY61 pKa = 10.12 TRR63 pKa = 11.84 TLKK66 pKa = 11.14 GLVTVEE72 pKa = 5.25 FIRR75 pKa = 5.3
Molecular weight: 8.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 10.043
IPC_protein 10.833
Toseland 11.067
ProMoST 11.096
Dawson 11.14
Bjellqvist 10.847
Wikipedia 11.345
Rodwell 11.462
Grimsley 11.169
Solomon 11.286
Lehninger 11.257
Nozaki 11.038
DTASelect 10.847
Thurlkill 11.052
EMBOSS 11.477
Sillero 11.067
Patrickios 11.199
IPC_peptide 11.301
IPC2_peptide 9.765
IPC2.peptide.svr19 8.393
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
90
0
90
16804
52
1176
186.7
20.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.879 ± 0.375
1.47 ± 0.148
5.689 ± 0.258
6.427 ± 0.266
3.678 ± 0.184
6.873 ± 0.273
1.845 ± 0.191
6.576 ± 0.176
6.76 ± 0.369
7.855 ± 0.273
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.785 ± 0.139
5.04 ± 0.199
3.815 ± 0.237
4.065 ± 0.276
4.838 ± 0.227
7.79 ± 0.291
5.392 ± 0.267
6.308 ± 0.261
1.535 ± 0.111
3.38 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here